Siyabonga ngokuvakashela i-Nature.com.Inguqulo yesiphequluli oyisebenzisayo inosekelo olulinganiselwe lwe-CSS.Ukuze uthole ulwazi olungcono kakhulu, sincoma ukuthi usebenzise isiphequluli esibuyekeziwe (noma ukhubaze i-Compatibility Mode ku-Internet Explorer).Okwamanje, ukuze siqinisekise ukwesekwa okuqhubekayo, sizonikeza isayithi ngaphandle kwezitayela ne-JavaScript.
Inhloso yalolu cwaningo bekuwukuhlola izinga lokuhoxiswa kwe-canine ngemithi emibili ye-laser therapy (LLLT) emibili ye-irradiation, okuhlanganisa amafrikhwensi aphezulu naphansi.Iziguli ezingamashumi amabili zazihlukaniswe ngokungahleliwe zaba amaqembu amabili.Eqenjini A, uhlangothi olulodwa lwe-maxillary arch lwahlelwa ngokungahleliwe ukuze lwamukele i-LILT ngezinsuku ezingu-0, 3, 7, 14, futhi njalo ngemva kwamaviki angu-2, kuyilapho kuqembu B, uhlangothi olulodwa lwathola i-LILT njalo emavikini angu-3.Phakathi nenkathi yokufunda yamasonto angu-12, ukuhamba kwamazinyo kwakubhekwa njalo ngemva kwamasonto amathathu kusukela ekuqaleni kokuhoxiswa kwe-canine.Ngaphezu kwalokho, amazinga e-interleukin-1β (IL-1β) ku-gingival sulcus fluid ahlolwe. Imiphumela yembula ukukhuphuka okuphawulekayo kwesilinganiso sokuhlehla kwe-canine ezinhlangothini ze-laser zamaqembu A no-B, uma kuqhathaniswa nezinhlangothi zokulawula (p <0.05), ngaphandle komehluko obalulekile obikiwe phakathi kwezinhlangothi ze-laser kuwo womabili amaqembu (p = 0.08-0.55). Imiphumela yembula ukwanda okuphawulekayo kwezinga lokuhlehlisa i-canine ezinhlangothini ze-laser zamaqembu A no-B, uma kuqhathaniswa nezinhlangothi zokulawula (p <0.05), ngaphandle kokwehluka okuphawulekayo okubikiwe phakathi kwezinhlangothi ze-laser kumaqembu womabili (p = 0.08-0.55). Результаты выявили значительное увеличение скорости ретракции клыков на стороне лазера в группах A and B по сравнению с контрольной стох0 различий между сторонами лазера в обеих группах (p = 0,08–0,55). Imiphumela yembule ukwanda okuphawulekayo kwejubane lokuhlehlisa i-canine ohlangothini lwe-laser kumaqembu A no-B uma kuqhathaniswa nohlangothi lokulawula (p <0.05), ngaphandle komehluko omkhulu phakathi kwezinhlangothi ze-laser kuwo womabili amaqembu (p = 0.08-0.55). ).结果显示，与对照组相比，A 组和B 组激光侧的犬齿回缩率显着增加（p <0.05光侧的犬齿回缩率显着增加（p <0.05显显漉伉， Ikhasi (p = 0.08-0.55).结果 显示 , 与 对照组 , 组和 a 组和 b 组 激光侧 犬齿 回 缩率 显着 （（组 激光侧 犬齿 回 缩率 显着 （（组激光侧 犬齿 回 缩率 显着（（组激光侧 犬齿回)间 显着 差异（p = 0.08-0.55。。。. Результаты показали, что по сравнению с контрольной группой скорость ретракции клыков на стороне лазера в группах А и В быльков на стороне лазера в группах А и В была зpто не лазера не было существенной разницы между двумя группами (p = 0,08-0,55). Imiphumela yabonisa ukuthi, uma kuqhathaniswa neqembu lokulawula, izinga lokuhoxiswa kwe-canine ohlangothini lwe-laser emaqenjini A no-B laliphakeme kakhulu (p <0.05), futhi kwakungekho umehluko omkhulu phakathi kwamaqembu amabili ohlangothini lwe-laser (p = 0.08-0 .55). Futhi, amazinga e-IL-1β ayephakeme kakhulu ezinhlangothini ze-laser zamaqembu womabili, uma kuqhathaniswa nezinhlangothi zokulawula (p <0.05). Futhi, amazinga e-IL-1β ayephakeme kakhulu ezinhlangothini ze-laser zamaqembu womabili, uma kuqhathaniswa nezinhlangothi zokulawula (p <0.05). Кроме того, уровни IL-1β были значительно выше на стороне лазера в обеих группах по сравнению с контрольной стороной (p <0,05). Ngaphezu kwalokho, amazinga e-IL-1β ayephakeme kakhulu ohlangothini lwe-laser kuwo womabili amaqembu uma kuqhathaniswa nohlangothi lokulawula (p <0.05).此外，与对照组相比，两组激光侧的IL-1β 水平显着升高（p <0.05）.此外，与对照组相比，两组激光侧的IL-1β 水平显着升高（p <0.05）. Кроме того, уровни IL-1β были значительно повышены на стороне лазера в обеих группах по сравнению с контрольной группой (p <0,00). Ngaphezu kwalokho, amazinga e-IL-1β aphakanyiswe kakhulu ohlangothini lwe-laser kuwo womabili amaqembu uma kuqhathaniswa neqembu lokulawula (p <0.05).Ngakho-ke, i-LILI yakwazi ukusheshisa ngokuphumelelayo ukunyakaza kwamazinyo, kungakhathaliseki ukuthi isetshenziswe kaningi noma ngokungajwayelekile, eyayihlotshaniswa nokusabela okwenyuka kwezinto eziphilayo, okwakubonakala emazingeni okwanda kwe-IL-1β ohlangothini olucindezelwe.
Ukwelashwa kwe-orthodontic yesikhathi eside (imvamisa ezungeze izinyanga ezingama-20-301) kutholakale ukuthi kunomthelela omubi ekuthotshelweni kwesiguli, ngaphezu kwezingozi ezifana ne-root resorption2, i-caries3, i-enamel decalcification3 kanye nezinkinga ze-periodontal4,5.Ngakho-ke, kuye kwahlongozwa izindlela eziningana okuhloswe ngazo ukusheshisa ukunyakaza kwamazinyo e-orthodontic (OTM), kuhlanganise nokunakekelwa kokuhlinzwa nokungahlinzeki.Ukwengeza, umphumela wokuhlanganisa izindlela ezimbili zokusheshisa kanye nomphumela wokuphinda inqubo efanayo yokusheshisa ngesivinini se-OTM6 yaphenywa.
I-Low intensity laser therapy (LLLT) kube enye yezindlela ezihlongozwayo ezingahlinzeki zokusheshisa i-OTM, kodwa kube nemiphumela engqubuzanayo emibikweni yokusebenza kwayo kahle kule ndawo, kuyilapho imiphumela emihle7,8 kanye ne-negative9 ibhaliwe.Le miphumela engqubuzanayo ingachazwa umehluko wemingcele yokusetshenziswa kwe-laser esetshenziswa ocwaningweni ngalunye, okuhlanganisa uhlobo lwe-laser, indlela yokusebenzisa, ubude begagasi, umthamo wemisebe, nesikhathi sokuchayeka, njengoba le mingcele ihlobene ngokuqondile nemiphumela yomtholampilo yokusetshenziswa kwe-laser 10.
Ngokuphathelene nezindlela zokufaka isicelo, kuye kwabikwa ukuthi izinqubo ezihlukahlukene ze-laser irradiation zisiza ukunyakaza kwamazinyo.Iphrothokholi eyodwa esetshenziswa kabanzi ihlanganisa ukusebenzisa i-laser ngezinsuku ezingu-0, 3, 7, 14, 21 kanye no-30, kuphinda ukulandelana okufanayo nyanga zonke, futhi le nqubo yomthetho yamukelwe ababhali abaningana11,12.Abanye basebenzise enye irejimeni esondele kakhulu kuhlobo oluchazwe ngaphambilini futhi engenye yezindlela ezisetshenziswa kakhulu, lapho i-LILI isetshenziswa ezinsukwini ezi-0, 3, 7, 14, bese kuba njalo ezinsukwini eziyi-15 kuze kube sekupheleni kwesikhathi socwaningo.13. Ngaphezu kwalokho, kuye kwahlongozwa iphrothokholi ehlanganisa ukusetshenziswa kwamasonto onke kwelaser enezinga eliphansi phakathi naso sonke isikhathi sokuhoxiswa kwezinja.Kodwa-ke, okubi okuyinhloko kwalezi zivumelwano ezivamile izinga eliphezulu lempendulo yesiguli, okungase kube nzima kuwo wonke umuntu.Ngakho-ke, izivumelwano ezidinga ukudluliselwa kweziguli ezimbalwa ziyasetshenziswa, isibonelo, kufaka phakathi i-LILI izikhathi ezingu-8 ngenyanga noma 15, 16, 17, 18 njalo emavikini angu-3.
Njengoba amandla e-orthodontic aziwa ukuthi adala ukuguqulwa kwamathambo, ukuthuthukiswa kwezinguquko zokuvuvukala kuyisidingo sale nqubo, okuholela ekulimazeni amazinyo19.Ngokwezifundo eziningana, enye indlela yokuhlola izenzakalo zebhayoloji ezingase zibe khona emthanjeni we-periodontal ukuhlola izinga lama-cytokines ku-gingival sulcus fluid (GCF).I-Interleukin-1β (IL-1β) iyi-cytokine esebenzayo kakhulu e-bone metabolism futhi ibhekwa njengenye ye-cytokines enamandla kakhulu ekuqaleni kwezicubu ze-periodontal ze-OTM.Njengoba kukhona ukuhlobana phakathi kwamazinga e-IL-1β kanye nokusinda, ukuhlanganiswa nokusebenza kwe-osteoclast, i-IL-1β ingabhekwa njengophawu olubalulekile lokubala izinga lokunyakaza kwamazinyo e-orthodontic, okuhlobene nokusebenza kahle kwe-alveolar bone remodeling24.
Ngakho-ke, inhloso yocwaningo lwethu kwakuwukuhlola nokuqhathanisa imiphumela ye-NILT nemithi evame ukusetshenziswa, okuhlanganisa imvamisa ephezulu yokusetshenziswa ezinsukwini ezingu-0, 3, 7, 14, bese kuba njalo emavikini angu-2 uma kuqhathaniswa nokusetshenziswa njalo emavikini angu-3.Izinga lokuhoxa ezinjeni ngomzamo wokunciphisa imvamisa yokukhumbula kwesiguli.Ngaphezu kwalokho, amazinga e-IL-1β ku-GCF ahlolwe kusetshenziswa izivumelwano ezimbili.I-null hypothesis yocwaningo lwamanje iwukuthi awukho umehluko ezenzakalweni zokuhoxiswa kwe-canine ne-LILI kusetshenziswa izivumelwano ezimbili zokuhlola.
Ucwaningo lwaluyisivivinyo somtholampilo esilawulwa ngokungahleliwe esinamaqembu amabili afanayo, ngalinye lihlola iphrothokholi ye-LILI.Iqembu ngalinye lamukela umklamo womlomo ohlukene, uhlangothi olulodwa yiqembu lokulawula kanti olunye yiqembu locwaningo.
Ucwaningo lwaluhlanganisa iziguli ze-20 ezineminyaka engu-15 kuya kwengu-20 ezazidinga ukususwa kokwelapha kwe-premolars yokuqala yomhlathi ongenhla, okulandelwa ukuhoxiswa kwama-canines.Izibalo zosayizi wesampula bezisekelwe ephutheni le-alpha elingu-5% namandla okufunda angu-80%.Lesi sibalo sisekelwe ekuphambukeni okujwayelekile nokujwayelekile kokuhoxiswa kwe-canine ezifundweni lapho u-Doshi-Mehta no-Bhad-Patil7 basebenzise i-LILI ngezinsuku ezingu-0, 3, 7, 14 kanye njalo emavikini angu-2 ngemva kwalokho (Ingalo A) kanye nasezifundweni ze-Qamruddin et al.abanye Ezifundweni eziyi-15, i-LILI yasetshenziswa njalo emavikini ama-3 (iqembu B).Ukugunyazwa kwezimiso zokuziphatha kwatholwa ku-Ethics Council of the Faculty of Dentistry, Alexandria University, Alexandria, Egypt (IRB: 00010556-IORG: 0008839).Inombolo yekomidi lezimiso zemibhalo yesandla ithi 0111-01/2020.Kugunyazwe ngoJanuwari 21, 2020. Ukuhlolwa kubhaliswe ne-ClinicalTrials.gov njengokuthi “Amaphrothokholi Amabili Eleveli Aphansi Okuhlola Isivinini Sokuhoxisa Ezinjeni.”Inombolo yokubhalisa yesilingo ithi NCT04926389.Usuku lokubhaliswa kwesilingo umhla ka-06/15/2021 kokuthi https://clinicaltrials.gov/ct2/show/NCT04926389.Ukubhaliswa kweziguli ocwaningweni kwaqala ngoFebhuwari 5, 2020 futhi kwaphela ngoNovemba 28, 2021.
Iziguli zaqashwa emtholampilo wamazinyo we-Faculty of Dentistry yase-Alexandria University.Izihloko zahlolwa futhi zahlolwa ngokuhambisana nalezi zindlela zokufaneleka ezilandelayo: impilo evamile, ukungabikho kwesifo esingapheli, akukho ukwelashwa kwangaphambili kwamazinyo, ukuhlanzeka komlomo okwanele, kanye nezicubu ze-periodontal ezinempilo.Iziguli ezibambe iqhaza kanye nabazali bazo banikezwe incazelo egcwele neningiliziwe yezinqubo zocwaningo, ngakho-ke, imvume enolwazi yatholwa esihlokweni ngasinye esifakiwe.Zonke izinqubo zocwaningo zenziwa ngokuhambisana neziqondiso ezifanele kanye nemithetho ebekwe kuSimemezelo sase-Helsinki.
Ngaphambi kokuqala ukuhoxiswa kwe-canine, iziguli ze-20 zikhethiwe futhi zabelwa ngokungahleliwe eqenjini A noma iqembu B (i-10 eqenjini ngalinye) ukuze uthole ukwelashwa kwe-laser okuphansi.Ukungahleliwe kwenziwe kusetshenziswa inqubo ye-randomization elula enesilinganiso sokusabalalisa esingu-1:1.Kwalungiselelwa ibhokisi elinamaphepha asongwe angamashumi amabili, ayishumi kuwo ayebhalwe amagama athi “Iqembu A” kanti amanye ayishumi anamagama athi “Iqembu B”.Umhlanganyeli ngamunye wacelwa ukuba akhethe iphepha eligoqiwe ebhokisini alinikeze elinye lamaqembu amabili ngendlela efanele.Inqubo efanayo yaphindwa futhi eqenjini ngalinye, kuqokwa uhlangothi olulodwa lwe-maxillary arch njengokuthi "ukuhlola" nolunye uhlangothi njengokuthi "ukulawula" ekwakhiweni komlomo ohlukanisiwe.
Ukwengeza kumarekhodi avamile we-orthodontic (izithombe ze-intraoral kanye ne-extraoral, ama-radiographs, nokuvela kwamazinyo), izifundo ezilungiselelwe ukwelashwa kwamazinyo okungaguquki zabhaliswa ngokuhlanganisa umlando wazo wezokwelapha nowamazinyo.Iziguli ziphinde zacelwa ukuba zihlanze umlomo futhi zipholishwe ngokuphelele kulandelwa iziyalezo zenhlanzeko efanele yomlomo (ukusetshenziswa kwesixubho, ifloss kanye namabhulashi aphakathi kwamazinyo).
Ukulungiswa kwe-maxillary kanye ne-mandibular enezintambo eziqondile zikagesi ze-Roth (i-Mini 2000; i-Ormco, e-USA) enezikhala ezingu-0.022″\(\x)0.028″ zalungiswa kuzo zonke iziguli ezibuthiwe, lapho inqubo yokulungisa yalungiswa kuwo womabili amaqembu futhi yanqunywa u-opharetha ofanayo..Kamuva, isiguli sathunyelwa ukuze kukhishwe i-premolar yokuqala ye-maxillary ukuze kuvunyelwe isikhathi esanele sokuthi isokhethi liphulukise ngemva kokukhipha ngaphambi kokuqala ukukhipha cishe izinyanga ezingu-2 ngemuva kokukhishwa.Ukuqondanisa bese kuqala futhi ukuqondanisa kuqedwe lapho intambo yensimbi engenasici engu-0.016″ x 0.022″ ingase ifakwe kancane kuwo wonke amazinyo amakhulu.
Ngaphambi kokuqala ukuhlehla kwe-canine, ama-premolars esibili aphezulu kanye ne-molars yokuqala ahlanganiswe ndawonye ngocingo lwe-0.009-inch figure-8 ezinhlangothini zokuhlola nokulawula zawo womabili amaqembu.Ukwengeza, ama-maxillary incisors ahlanganiswe ndawonye ngendlela efanayo nengxenye yangemuva ukusiza ukuzinza nokuvimbela ukuhlukana kwabo okungenzeka.
Ukuhlehla kweCanine ngamaqembu A no-B kwenziwa kusetshenziswa imithombo yekhoyili ye-nickel-titanium (NiTi) evaliwe (i-Ormco, e-USA), ezinhlangothini zokuhlola nokulawula, ezinwetshwe phakathi kwezingwegwe zamabakaki e-canine nezingwegwe ku-molar canal, ngamandla angu-150 g kulinganiswa i-dynamometer (Morelli, Brazil).
Ilaser diode (Wiser; Doctor Smile-Lambda Spa, Brendol, Italy) yasetshenziswa njengelaser enezinga eliphansi, ikhipha imisebe ye-infrared enobude begagasi obungu-980 nm namandla okukhipha angu-100 mW kumodi eqhubekayo.I-fiber wave wave (AB 2799; Doctor Smile-Lambda Spa, Brendola, Italy) yasetshenziswa ukuze kusatshalaliswe indawo ye-beam engu-1 cm2 enesihloko esiyisicaba esingaphezulu, imise ithiphu le-fiber eduze kwe-maxillary arch maphakathi nesithathu kwe-maxilla.impande ye-canine ohlangothini lokuhlola (ngokwemiyalo yomkhiqizi, ubuncane obungu-1.5 cm lapho ingagxilile) imizuzwana engu-8 (Fig. 1).Isamba samandla esisetshenziswa isiqephu ngasinye sasingu-8 J/cm2 (1 J/cm2 ngomzuzwana).Imingcele ye-laser esetshenzisiwe ikhonjiswe kuThebula 1. Ukuqapha kwathathwa ngaphambi kokusebenzisa i-laser, futhi isiguli kanye no-opharetha basebenzisa izibuko ezinikezwe umenzi, kuye ngobude begagasi obusetshenzisiwe.
I-fiber tip yayibanjwe ebangeni elingu-1.5 cm ukusuka empandeni ye-maxillary canine ohlangothini lokuhlola ngokusho kwemiyalelo yomkhiqizi.
Inqubo yokuhlukaniswa komlomo yasetshenziswa kuwo womabili amaqembu, futhi umhlanganyeli ngamunye wayehlelwe ngokungahleliwe ukuze athole i-LILI ngakolunye uhlangothi lwe-maxillary arch futhi ngakolunye uhlangothi njengezilawuli.Eqenjini A, izifundo zithole i-LILT ngezinsuku ezingu-0, 3, 7, 14 bese kuthi njalo emavikini angu-2, kuyilapho eqenjini B lalisetshenziswa njalo emavikini e-3 ohlangothini lokuhlola kuso sonke isikhathi sokufunda (amaviki angu-12) we-LILT.I-laser beam nayo yalungiswa ngokungenzi lutho ohlangothini lokulawula lwamaqembu womabili, ihlinzeka ngomphumela we-placebo njengengxenye yenqubo yokuphuphuthekisa iziguli ezibhalisiwe.Ngenxa yemvelo yokungenelela kulesi sigaba, u-opharetha akakwazi ukukhohliswa.
Ngaphambi kokuqoqwa kwe-specimen, izinhlangothi zombili zama-maxillary canines zazihlanzwa nge-cotton swabs, zihlukaniswe ngama-retractors azisekelayo, ama-suction, nama-cotton rolls, bese omiswa ngomoya omnene amasekhondi angu-5.Amasampula athathwe emifantwini ekude yama-maxillary canines kusetshenziswa amapheshana ajwayelekile okuhlunga amaphepha (Whatman, Maidstone, UK) futhi asikwa abe osayizi abajwayelekile be-2 × 10 mm2.Faka ngobumnene umugqa ngamunye esikhaleni kuze kube yilapho uzwa ukumelana kancane, bese uwushiya endaweni imizuzwana engama-60 ngenkathi ugcina ukuvalwa okufanele (Umfanekiso 2).Ngemuva kokususwa, amapheshana amasha afakwa njalo ngeminithi elingu-1 ukuze kutholwe imicu emi-4 endaweni ngayinye.Izinyathelo nazo zathathwa ukuze kugwenywe ukulimala komshini emfantwini we-gingival.Lahla amasampula angcoliswe amathe noma igazi bese uqoqa amasampula amasha.Amasampula e-GCF athathwe ekuqaleni (ngaphambi kokuqala kokuhoxiswa kwe-canine), kusukela emifantwini ye-distal canine emaqenjini A no-B, ezinhlangothini zokuhlola nokulawula, ngaphandle kwezinsuku ezingu-7, 14, no-21.
Imibono ye-Alginate (i-Ca37; i-Cavex, i-Haarlem, i-Netherlands) yenziwa ngaphambi kokuhoxiswa kwe-canine futhi yayiphindaphindiwe njalo emavikini e-3 phakathi nocwaningo lwe-12-isonto ekuvakasheleni ngakunye.Ekuvakasheni ngakunye, izintambo zocingo neziphethu zamakhoyili zazikhishwa, kuthathwe isithombe se-alginate, bese kubalwa.Umfuziselo wezinyo ube ususikwa futhi ubhalwe igama lesiguli, inombolo nosuku.Imodeli ye-plaster yabe isiskeniwa (isithwebuli selabhorethri ye-inEos X5 CAD/CAM; Dentsply Sirona, PA, USA) ukuze kwakhiwe isithombe sedijithali esinezinhlangothi ezintathu (3D) semodeli yamazinyo.Izilinganiso ezidingekayo zenziwe kusetshenziswa inguqulo ye-AutoCAD 2013 (AutoCAD; Autodesk, USA).Odokotela babengaqapheli izinhlangothi zokuhlola nokulawula ngesikhathi sokulinganisa ukuze kugwenywe ukuchema okungenangqondo, futhi ukuhlola ukwethembeka komphenyi we-intra-investigator kwenziwa ngezilinganiso eziphindaphindiwe ngumqhubi ofanayo ngesonto kamuva ukuhlola amaphutha okulinganisa.Iphutha elilinganiselwe lesilinganiso ngu-6%.
Kutholwe izimpawu zendawo ezimbalwa ekulingisini kwamazinyo, okuhlanganisa i-palatal suture emaphakathi, amaphuzu amaphakathi wokugoqa wesithathu kwesokunxele nakwesokudla, kanye nama-cusps ama-maxillary canines kwesokunxele nakwesokudla.Umugqa oqondile usuka emaphoyinti aphakathi kokugoqeka kwesithathu kwesokunxele nakwesokudla kanye nama-tubercles we-maxillary canines kwesokunxele nakwesokudla kuya kumthungo we-palatine ophakathi.Izilinganiso zangaphambili-ngemuva zathathwa phakathi komugqa we-canine wamazwe amabili kanye nomugqa wesithathu wokugoqa ukuze kuhlolwe ukuhoxiswa kwe-canine (Amakhiwane 3, 4).
Thola izimpawu zendawo ezithombeni eziskeniwe zamamodeli wamazinyo ukuze ulinganise ukuhoxiswa kwe-canine.(Eyodwa).I-palatal suture ephakathi.(b, d).Ama-Tubercles we-maxillary canines kwesokunxele nakwesokudla, ngokulandelana.(c, e).Imigqa ehambisana neziphetho zangaphakathi zokugoqa kwesithathu kwesokunxele nakwesokudla, ngokulandelana.
Ngemva kokususwa emfantwini we-gingival, amaqembu emicu yephepha yesihlungi eqoqwe endaweni eyodwa afakwa kumashubhu e-Eppendorf (Capp, Denmark) aqukethe u-100 µl we-saline evikelwe yi-phosphate.Amashubhu e-Eppendorf aye avalwa futhi alebula futhi amasampula ngokushesha afakwa centrifuged ku-3000 rpm imizuzu engu-10 kusetshenziswa i-centrifuge (Hettich Universal 320R BC-HTX320; GMI, MN, USA) ukuze kubuyiselwe amasampula e-GCF emicu.Amashubhu e-Eppendorf agcinwa ku -20 ° C kuze kube yilapho kuhlaziywa amakhemikhali e-biochemical.Ukuhlaziywa kwamazinga e-IL-1β kwenziwa kusetshenziswa i-enzyme-linked immunosorbent assay (ELISA; Cloud-Clone, Howe, USA).Ukugxila kwe-IL-1β kwanqunywa ngokuqhathanisa ukuminyana kokubona (OD) kwamasampuli atholiwe nejika elijwayelekile futhi ngokufanele kubalwe isibalo sokuhlehla komugqa kwejika elijwayelekile.Ekugcineni, imiphumela yamazinga e-IL-1β yethulwa ku-pg/ml/60 s25.I-flowchart yomklamo wocwaningo ikhonjisiwe kuMfanekiso 5, ofinyeza inqubo yocwaningo.
Ukuhlaziywa kwezibalo kwenziwa kusetshenziswa i-IBM SPSS yenguqulo ye-Windows 23.0 (IBM; Armonk, NY, USA).Zonke izinto eziguquguqukayo zobuningi bezivame ukusatshalaliswa futhi zisho ukuthini, ukuchezuka okujwayelekile (SD) kanye nesikhawu sokuzithemba esingu-95% (CI) kubalwe futhi kwasetshenziswa izivivinyo zepharamitha.Okuguquguqukayo okulinganiselwe (ukuhoxiswa kwe-canine kanye nezinga le-IL-1β) kuqhathaniswe phakathi kwamaqembu amabili ocwaningo esebenzisa isampula yokuhlola ye-t-test, kuyilapho ukuqhathanisa phakathi kwe-laser nezinhlangothi zokulawula eqenjini ngalinye kwenziwa kusetshenziswa ukuhlolwa kwe-t okubhangqiwe.Ukuhoxiswa kwenja kanye namazinga e-IL-1β ngezikhathi ezihlukene eqenjini ngalinye kwaqhathaniswa ngokuhlukana kusetshenziswa ukuhlaziya okuphindaphindiwe kwezilinganiso zokuhlukahluka okulandelwa ukuqhathanisa okuningi okubili kusetshenziswa amazinga okubaluleka alungisiwe e-Bonferroni. Ukubaluleka kusethwe kunani elingu-p <0.05. Ukubaluleka kusethwe kunani elingu-p <0.05. Значимость была установлена ​​при значении p <0,05. Ukubaluleka kusethwe kunani elingu-p <0.05.显着性设定为p 值< 0.05.显着性设定为p值< 0.05. Значимость была установлена ​​на уровне p <0,05. Ukubaluleka kusethwe ku-p<0.05.
Ngesikhathi socwaningo, asikho isifundo esiyekile phakathi nesikhathi sangaphambi kokungenelela noma phakathi nesalela socwaningo.Zonke izifundo ze-20 eziqashwe ekuqaleni zaqeda sonke isikhathi sokufunda samasonto e-12 (izifundo ze-10 ngeqembu ngalinye).Ukugeleza kwesiguli kuso sonke isilingo kuboniswa kuMfanekiso 6 kusetshenziswa i-flowchart ye-CONSORT.Idatha yezibalo zabantu ezifundweni ezibhaliswe kuQembu A no-B yethulwa kuThebula 2. Azikho izimo ze-prolapse kumamodeli ocwaningo, ayenziwa njalo emavikini amathathu ukukala ukuhoxiswa kwe-canine.Ngaphezu kwalokho, wonke amasampula e-GCM atholiwe acutshungulwa ngokucophelela futhi ahlaziywa.
Inani lokuhlehla kwe-maxillary canine ngezikhathi ezihlukene lichazwe kuThebula 3, ngokuphathelene womabili amaqembu A no-B. Eqenjini A, ibanga elikhulu kakhulu elinesilinganiso (± SD) elihanjwa i-maxillary canine libikwe evikini lesi-3 libe ngu-1.18 (± 0.04) mm ohlangothini lwe-laser, kanye no-0.85 (± mm 0.04 ohlangothini olubalulekile, umehluko obalulekile phakathi kwawo no-0.04 ohlangothini olubalulekile) 1). Inani lokuhlehla kwe-maxillary canine ngezikhathi ezihlukene lichazwe kuThebula 3, ngokuphathelene womabili amaqembu A no-B. Eqenjini A, ibanga elikhulu kakhulu elinesilinganiso (± SD) elihanjwa i-maxillary canine libikwe evikini lesi-3 libe ngu-1.18 (± 0.04) mm ohlangothini lwe-laser, kanye no-0.85 (± mm 0.04 ohlangothini olubalulekile, umehluko obalulekile phakathi kwawo no-0.04 ohlangothini olubalulekile) 1). Величина ретракции верхнечелюстного клыка в разные моменты времени описана в таблице 3 для обеих групп А и В.В группе А наибольшее среднее расстояние (± SD), пройденное верхнечелюстным клыком 3-й неделе, составляет 1,18 (± 0m0 8, ла ,04) мм на стороне контроля, при этом разница между ними статистически значима (p < 0,001). Inani lokuhlehla kwe-maxillary canine ngezikhathi ezihlukene lichazwa kuThebula 3 kuwo womabili amaqembu A no-B. Eqenjini A, ibanga elide kakhulu elinesilinganiso (± SD) elihanjwa i-maxillary canine ngeviki lesi-3 lingu-1.18 (± 0.04) mm ohlangothini lwe-laser kanye no-0.85 (± 0.04) mm phakathi kohlangothi olubalulekile (± 0.04) mm phakathi kohlangothi olubalulekile (0.0 p.Kumaqembu A no-B, izinga lokuhlehla kwe-maxillary canine ngezikhathi ezihlukene lichazwe kuThebula 3.在 A 组中，上颌尖牙移动的最大平均距离(± SD) 在第3 周报告為激光侧為1.18 (± 0.04) = ± 0.04) mm，诹间的差异具有统计学意义（p <0.001）。在 a 组 中 ， 上 颌 移动 的 最 大 距离 距离 距离 在 第 3 周 报告 為激光 侧 為 1.0 18 × 8 × 8 mm. ± 0.04 mm有 具有 具有 具有 具有 具有 具有 具有 具有统计学意义（p <0.001）. В группе А максимальное среднее расстояние (± SD) движения клыков верхней челюсти на 3-й неделе составило 1,18 (± 0,04) м0 , см 50, 5 (± 0,04) м0 м на стороне контроля, разница между ними была Статистическая значимость (p <0,001). Eqenjini A, ibanga lesilinganiso esiphezulu (± SD) sokunyakaza kwe-maxillary canine ngeviki lesi-3 lalingu-1.18 (± 0.04) mm ohlangothini lwe-laser kanye no-0.85 (± 0.04) mm ohlangothini lokulawula, umehluko phakathi kwawo wawubalulekile ngokwezibalo (p <0.001). Kodwa-ke, inani elilinganiselwe lokunyakaza kwamazinyo lehla ngeviki le-6 kuzo zombili i-laser nezinhlangothi zokulawula, bese landa kancane kancane ngemva kwamasonto angu-9 kanye ne-12, nenani lokunyakaza kwamazinyo liphakeme kakhulu ohlangothini lwe-laser uma kuqhathaniswa nohlangothi lokulawula (p <0.001), kuzo zonke izikhathi amaphuzu. Kodwa-ke, inani elilinganiselwe lokunyakaza kwamazinyo lehla ngeviki le-6 kuzo zombili i-laser nezinhlangothi zokulawula, bese landa kancane kancane ngemva kwamasonto angu-9 kanye ne-12, nenani lokunyakaza kwamazinyo liphakeme kakhulu ohlangothini lwe-laser uma kuqhathaniswa nohlangothi lokulawula (p <0.001), kuzo zonke izikhathi amaphuzu.Kodwa-ke, inani elilinganisiwe lokususwa kwamazinyo lehla ngeviki lesi-6 kuzo zombili i-laser nezinhlangothi zokulawula, bese lenyuka kancane kancane phakathi namaviki 9 kanye ne-12, nenani lokususwa kwezinyo libe phezulu kakhulu ohlangothini lwe-laser.i-laser uma iqhathaniswa neqembu lokulawula.стороны (p < 0,001) во все моменты времени. side (p <0.001) ngaso sonke isikhathi.然而，激光侧和对照侧的平均牙齿移动量在第6 周下降，然后在第9周和第12 周希帎帅民宿激光侧的牙齿移动量明显更高侧（p <0.001），在所有时间点.然而 , 激光 和 对照侧的 牙齿 移动量 在第 6 周 下降 , 然后第 第 第 第 第 第 1增加 , 与 对照 相比 , 的 移动量 明显 更 高侧 （p <0.001），在所有时间点. Однако среднее количество движений зубов на стороне лазера и контрольной стороне уменьшилось на 6-й неделе, а затем постепененно увели12зько 9 движений зубов на стороне лазера было значительно выше по сравнению с контрольной стороной (p <0,001) во все моменты времени. Kodwa-ke, inani elilinganiselwe lokunyakaza kwamazinyo ohlangothini lwe-laser kanye nohlangothi lokulawula lwehla ngeviki lesi-6 bese landa kancane kancane ngemva kwamasonto angu-9 no-12, futhi inani lokunyakaza kwamazinyo ohlangothini lwe-laser laliphakeme kakhulu uma liqhathaniswa nohlangothi lokulawula (p<0.001) kuwo wonke amaphuzu ngesikhathi. Inani eliphelele lokunyakaza kwamazinyo (± SD) esikhathini sokufunda samasonto angu-12 laliphezulu kakhulu ohlangothini lwe-laser ngo-4.45 (± 0.13) mm, uma kuqhathaniswa nalelo ohlangothini lokulawula olwalungu-3.16 (± 0.14) mm (p <0.001). Inani eliphelele lokunyakaza kwamazinyo (± SD) esikhathini sokufunda samasonto angu-12 laliphezulu kakhulu ohlangothini lwe-laser ngo-4.45 (± 0.13) mm, uma kuqhathaniswa nalelo ohlangothini lokulawula olwalungu-3.16 (± 0.14) mm (p <0.001). Ukushintshanisa okungcono kakhulu kwe- SD (± SD) ku-12-недельный период исследования была значительно выше на стороне лазера – 4,43 (± 0, сько, собоя) роной, которая составляла 3,16 (± 0,14) мм (p < 0,001). Inani eliphelele lokususwa kwamazinyo (± SD) esikhathini sokufunda samasonto angu-12 laliphezulu kakhulu ohlangothini lwe-laser, 4.45 (± 0.13) mm, uma kuqhathaniswa nohlangothi lokulawula, olwaluyi-3.16 (± 0.14) mm (p <0.001).在为期12 周的研究期间，激光侧的牙齿移动总量(± SD) 显着更高，為4.45 (± 0.13) mm，而对0 (4) mm，而对 0.1 mm (3p0 (1 mm) (3 p. 1).在 周 12 周周 研究研究, 激光侧激光侧 牙齿的 牙齿 牙齿牙齿 纻动 纻动纻动 (± sd) 显着 更 高, 为 4.45 (± 0.16 (± 0.16 (± 0.16) (± 0.16) (± 0.16) (± 0.16) В течение 12-недельного периода исследования общее перемещение зубов (± SD) было значительно выше на стороне лазера и соста, 415 3 соста, 4 5 ,16 (± 0,14) мм в контрольной группе (p < 0,001). Phakathi nenkathi yokufunda yamaviki angu-12, ukunyakaza kwezinyo okuphelele (± SD) kwakuphakeme kakhulu ohlangothini lwe-laser ku-4.45 (± 0.13) mm uma kuqhathaniswa ne-3.16 (± 0.14) mm eqenjini lokulawula (p <0.001).
KuQembu B, iphethini efanayo naleyo ekhonjiswe kuQembu A ilandelwe, ngamavelu aphezulu kakhulu okunyakaza kwamazinyo aqoshwa ohlangothini lwe-laser, uma kuqhathaniswa nohlangothi lokulawula kuzo zonke izikhathi (p <0.001). KuQembu B, iphethini efanayo naleyo ekhonjiswe kuQembu A ilandelwe, ngamavelu aphezulu kakhulu okunyakaza kwamazinyo aqoshwa ohlangothini lwe-laser, uma kuqhathaniswa nohlangothi lokulawula ngazo zonke izikhathi (p <0.001). В группе B наблюдалась аналогичная картина, продемонстрированная в группе A, со значительно более высокими значениями друппе лазера, по сравнению с контрольной стороной во все моменты времени (p <0,001). Iqembu B libonise iphethini efanayo neQembu A, elinamanani aphezulu kakhulu okunyakaza kwamazinyo aqoshwe ohlangothini lwe-laser uma kuqhathaniswa nohlangothi lokulawula ngazo zonke izikhathi (p <0.001).在B 组中，遵循与A 组相似的模式，与所有时间点的对照侧相比，激光侧记录的牙齿移动0 ）. <0.00 В группе B, по аналогии с группой A, зарегистрированные значения перемещения зубов были значительно выше на стороне лазения правда менты времени (p < 0,001). Eqenjini B, elifana neqembu A, amanani arekhodiwe okunyakaza kwamazinyo ayephezulu kakhulu ohlangothini lwe-laser uma kuqhathaniswa nohlangothi lokulawula ngazo zonke izikhathi (p <0.001).Ngemuva kwamaviki angu-3, ​​ukunyakaza kwamazinyo okuphezulu (± SD) kwarekhodwa ngenani elingu-1.14 (± 0.04) mm ohlangothini lwe-laser kanye no-0.87 (± 0.03) mm ohlangothini lokulawula.Ukuhamba kwamazinyo kamuva kwehla ngeviki lesi-6 futhi kancane kancane kwanda. Isamba senani lokuhlehla kwe-canine (± SD) esikhathini sokufunda samaviki angu-12 ku-laser nezinhlangothi zokulawula, lalingu-4.35 (± 0.12) mm, kanye no-3.10 (± 0.06) mm, ngokulandelana, futhi umehluko phakathi kwakho wawubalulekile ngokwezibalo (p <0.001). Isamba senani lokuhlehla kwe-canine (± SD) esikhathini sokufunda samaviki angu-12 ku-laser nezinhlangothi zokulawula, lalingu-4.35 (± 0.12) mm, kanye no-3.10 (± 0.06) mm, ngokulandelana, futhi umehluko phakathi kwakho wawubalulekile ngokwezibalo (p <0.001).Isamba sokuhlehla kwe-canine (± SD) esikhathini sokufunda samaviki angu-12 ku-laser nezinhlangothi zokulawula kube ngu-4.35 (± 0.12) mm no-3.10 (± 0.06) mm, ngokulandelana, futhi umehluko phakathi kwakho wawubalulekile ngokwezibalo .(р < 0,001). (p <0.001).在为期12 周的研究期间，激光侧和对照侧的犬齿回缩总量(± SD) 分别為4.35 (± 0.12) mm ± 3.10 (光) mm ± 3.10 (6.6)异具有统计学意义(p <0.001).在 為期 12 周 的 研究 ， 激光 侧 和 对照侧 的 回缩 总量 总量 总量 (± sd) 分和别 ± 3 0 6 .3 mm (± 3 0 4.3) (± 3 0 6) (3 mm 4). mm , 之间 的 差异 具有 统计学 意义 (p (p <0.001). В течение 12-недельного периода исследования общая (± SD) ретракция клыка на стороне лазера и контрольной стороне составила 4,35 (± 3, 10, мо), ± 3, 10 (± 10, мо) ответственно, и разница была статистически значимой (p <0,001). Phakathi nenkathi yokufunda yamaviki angu-12, isamba (± SD) ukuhoxiswa kwe-canine ohlangothini lwe-laser nohlangothi lokulawula kwaba ngu-4.35 (± 0.12) mm no-3.10 (± 0.06) mm, ngokulandelana, futhi umehluko wawubalulekile ngokwezibalo (p <0.001). .Ithebula lesi-4 lichaza ukuqhathaniswa kwezinga lokuhlehla kwe-canine ngezikhathi ezihlukene phakathi kwe-laser nohlangothi lokulawula eqenjini ngalinye locwaningo.
Nakuba izinga lokuhoxiswa kwe-canine nge-laser laliphezulu eqenjini A kunaseqenjini B ngaso sonke isikhathi amaphuzu, lo mehluko wawungabhekwa njengobalulekile ngokwezibalo uma uqhathaniswa neqembu B (p = 0.08-0.55).Ngokuphathelene nokwenyuka kwamaphesenti (± SD) ekuhoxisweni kwe-canine okuzuzwe ngephrothokholi ngayinye, umthetho olandelwayo osetshenziswe eqenjini A ukhuphuke ngo-40.78 (± 4.81)%, kuyilapho umthetho olandelwayo osetshenziswe eqenjini A ukhuphuke ngo-40 .22 (± 4.80) % eqenjini B. iphrothokholi yohlelo lokusebenza lwelaser yamukelwe.Nokho, nakuba leli phesenti lalingaphezulu kancane kuqembu A kuneqembu B, umehluko phakathi kwalo wawungabalulekile ngokwezibalo (p = 0.82).Ngaphezu kwalokho, kutholwe ukuthi uhlobo lokuhamba kwamazinyo kuwo womabili amaqembu luyafana (Fig. 7).
Ukuhlehla kwe-laser ye-lateral canine (mm) ngezikhathi ezihlukene kuwo womabili amaqembu ocwaningo phakathi nesikhathi sokufunda samaviki ayi-12.
Ithebula lesi-5 lichaza amazinga e-IL-1β emaqenjini A no-B kuwo wonke amaphoyinti esikhathi esilinganisiwe ku-laser nezinhlangothi zokulawula.Eqenjini A, umehluko phakathi kohlangothi lwe-laser nohlangothi lokulawula ekuqaleni ubungabalulekile kumanani we-IL-1β (p = 0.56). Izinga eliphakeme kakhulu le-IL-1β (± SD) larekhodwa ngosuku lwesi-7 kuzo zombili izinhlangothi ze-laser nezinhlangothi zokulawula, ngamavelu angu-0.152 (± 0.004) pg/ml/60 s, kanye no-0.127 (± 0.004) pg/ml/60 s, ngokulandelana, futhi umehluko obalulekile phakathi kwawo ngu-00p (obalulekile okungu-1p). Izinga eliphakeme kakhulu le-IL-1β (± SD) larekhodwa ngosuku lwesi-7 kuzo zombili izinhlangothi zelaser nezinhlangothi zokulawula, ngamanani angu-0.152 (± 0.004) pg/ml/60 s, kanye no-0.127 (± 0.004) pg/ml/60 s, ngokulandelana, futhi umehluko wawungu-10p obalulekile phakathi kwawo.Izinga eliphakeme kakhulu le-IL-1β (± SD) larekhodwa ngosuku lwesi-7 kuzo zombili izinhlangothi zelaser nezinhlangothi zokulawula ezinamanani angu-0.152 (± 0.004) pg/mL/60 s no-0.127 (± 0.004) pg/mL./60 с соответственно, а разница между ними была статистически значимой (p <0,001). /60 s, ngokulandelana, futhi umehluko phakathi kwawo wawubalulekile ngokwezibalo (p <0.001).在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD)，值為0.152 (± 0.004) pg/ml/60 s 和0.0g40ml (± 0.004) s 和0.0g40ml之间的差异具有统计学意义（p <0.001）.在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD)，值為0.152 (± 0.004) pg/ml/60 s 和0.0g120ml (± 0.004) s 和0.0g120ml ）.Ngosuku lwesi-7, amazinga aphakeme kakhulu e-IL-1β (± SD) aqoshwa kuzo zombili izinhlangothi ze-laser kanye nokulawula ngamanani angu-0.152 (± 0.004) pg/mL/60 s no-0.127 (± 0.004) pg/mL./60 imizuzwana.Разница между ними была статистически значимой (p <0,001). Umehluko phakathi kwabo ububalulekile ngokwezibalo (p <0.001). Ukwehla kancane kancane kumazinga e-IL-1β kuye kwabikwa ngemva kwalokho, ezinsukwini ezingu-14 no-21, kuzo zombili i-laser nezinhlangothi zokulawula, ngamavelu ohlangothini lwe-laser aphakeme kakhulu kunalawo asohlangothini lokulawula (p <0.001). Ukwehla kancane kancane kumazinga e-IL-1β kubikwe ngemuva kwalokho, ngezinsuku ze-14 no-21, kuzo zombili i-laser nezinhlangothi zokulawula, ngamavelu ohlangothini lwe-laser ephakeme kakhulu kunalawo asohlangothini lokulawula (p <0.001). I-Pосле этого сообщалось остепенном снижении уровней IL-1β ku-14 futhi 21 izinsuku как на стороне лазера, так и на стороне контроля, принито на земли тельно выше, чем на стороне контроля (p <0,001). Ngemuva kwalokho, ukwehla kancane kancane kumazinga e-IL-1β kwabikwa ezinsukwini ze-14 ne-21 kuzo zombili i-laser nezinhlangothi zokulawula, ngamavelu ohlangothini lwe-laser ephakeme kakhulu kunalawo asohlangothini lokulawula (p<0.001). .此后，在第14 天和第21 天，激光和对照侧的IL-1β 水平逐渐下降，激光侧的值显着高于对 <0 .此后，在第14 天和第21 天，激光和对照侧的IL-1β 水平逐渐下降，激光侧的值显着高于对的 I-ИЛ-1β iqala ngo-14 kuya ku-21 kuya ku-21 kuya ku-21 i-контроля (p <0,001). Ngemuva kwalokho, ngezinsuku ze-14 nezingu-21, amazinga e-IL-1β ehla kancane kancane ohlangothini lwe-laser nasekulawuleni, kuyilapho amanani ohlangothini lwe-laser ayephakeme kakhulu kunohlangothi lokulawula (p <0.001).
Eqenjini B, iphethini efanayo yabonwa eqenjini A ngokuphathelene namazinga e-IL-1β, ngokungafani okuncane okubonwa ekuqaleni phakathi kwe-laser nezinhlangothi zokulawula (p = 0.02). Ngemuva kwezinsuku ezingu-7, ukuphakama kwezinga le-IL-1β (± SD) kufinyelelwe kuzo zombili izinhlangothi, ngo-0.139 (± 0.004) pg/ml/60 s ohlangothini lwe-laser, kanye no-0.122 (± 0.003) pg/ml/60 s ohlangothini lokulawula, amanani acatshangelwe ngo-1.0. Ngemuva kwezinsuku ezingu-7, ukuphakama kwezinga le-IL-1β (± SD) kufinyelelwe kuzo zombili izinhlangothi, ngo-0.139 (± 0.004) pg/ml/60 s ohlangothini lwe-laser, no-0.122 (± 0.003) pg/ml/60 s ohlangothini lokulawula, ngamavelu acatshangelwa okuthi <1p 0.Ngemva kwezinsuku ezingu-7, izinga eliphakeme le-IL-1β (± ukuchezuka okujwayelekile) lafinyelelwa nhlangothi zombili: 0.139 (± 0.004) pg/ml/60 s ohlangothini lwe-laser kanye no-0.122 (± 0.003) pg/ml/60 s.на контрольной стороне, при этом значения на стороне лазера считались статистически более высокими (p <0,001). ohlangothini lokulawula, kuyilapho amanani ohlangothini lwe-laser ayebhekwa phezulu ngokwezibalo (p <0.001). 7天后，两侧达到IL-1β水平峰值（±SD），激光侧為0.139（±0.004）pg/ml/60 s，激光侧為0.1060p/s/ml/s)激光侧為0.100p/sg/s.g/s/s.制侧，激光侧的值在统计上更高（p <0.001). 7 天 后 , 两 侧 达到 达到 il-1β 水平 (（±） , 激光 侧 為 為 0.139 (± 0.004） pg/ml/06). 003） pg/ml/60 s 在侧 激光 激光 激光 激光 激光 激光 激光 激光 , , ,值在统计上更高 (p <0.001).Ngemuva kwezinsuku ezingu-7, amazinga aphezulu e-IL-1β (± SD) afinyelelwe ezinhlangothini zombili: 0.139 (± 0.004) pg/ml/60 s ohlangothini lwe-laser kanye no-0.122 (± 0.003) pg/ml/60 s ohlangothini lokulawula., лазер Значения на стороне были статистически выше (p < 0,001). , Amanani e-laser ohlangothini ngalunye ayephezulu ngokwezibalo (p <0.001).Amazinga e-IL-1β kamuva ancipha kancane kancane ezinhlangothini zombili ezinsukwini ze-14 ne-21, futhi amazinga aqoshwe ohlangothini lwe-laser ayephakeme kakhulu uma kuqhathaniswa nohlangothi lokulawula kuzo zombili amaphuzu wesikhathi (p = 0.001-0.002).Ukuqhathaniswa kwamazinga e-IL-1β kumaphoyinti esikhathi ahlukene phakathi kohlangothi lwe-laser nohlangothi lokulawula eqenjini ngalinye locwaningo kuchazwe kuThebula lesi-6.
Lapho kuqhathaniswa amazinga e-IL-1β phakathi kwamaqembu amabili ocwaningo, umehluko ongabalulekile uqoshwe ohlangothini lwe-laser ekuqaleni (p = 0.96). Ngosuku lwesi-7 nolwe-14, umehluko ophawulekayo ngokwezibalo ubhalisiwe phakathi kwezinhlangothi ze-laser kuwo womabili amaqembu, ngamavelu aphezulu angawasezinhlangothini ze-laser kuQembu A (p <0.001). Ngosuku lwesi-7 nolwe-14, umehluko ophawulekayo ngokwezibalo ubhalisiwe phakathi kwezinhlangothi ze-laser kuwo womabili amaqembu, ngamavelu aphezulu angawasezinhlangothini ze-laser kuQembu A (p <0.001). На 7-й и 14-й дни зарегистрированы статистически значимые различия между лазерными сторонами в обеих группах, причемысолезер болезев ным сторонам в группе А (р < 0,001). Ngosuku lwe-7 nolwe-14, kube nomehluko obalulekile ngokwezibalo phakathi kwezinhlangothi ze-laser kuwo womabili amaqembu, ngamavelu aphezulu okungezinhlangothi ze-laser eqenjini A (p <0.001).在第7 天和第14 天，两组激光侧的差异有统计学意义，A 组激光侧的值较高（p <0.001）. A На 7 and 14 дни разница между двумя группами была статистически значимой на стороне лазера с более высокими значениями на стопроне 10 . Ngosuku lwe-7 no-14, umehluko phakathi kwamaqembu amabili wawubaluleke ngokwezibalo ohlangothini lwe-laser, ngamavelu aphezulu ohlangothini lwe-laser eqenjini A (p <0.001).Ngemva kwezinsuku ezingu-21, kwakungekho umehluko omkhulu phakathi kwamaqembu amabili (p = 0.26).Amazinga we-IL-1β kuwo womabili amaqembu abe nomlingiswa ofanayo, afinyelela inani eliphakeme ngosuku lwe-7 futhi kancane kancane ehla ngezinsuku ze-14 ne-21 (Fig. 8).
Inhloso yalolu cwaningo ngokuyinhloko ukuhlola nokuqhathanisa umphumela we-LILR ekuhoxisweni kwe-canine usebenzisa iphrothokholi ehlanganisa i-high frequency laser irradiation ezinsukwini ezingu-0, 3, 7, 14 kanye njalo ngemva kwamaviki angu-2 ngemva kwalokho (Iqembu A) neziguli ezisanda kufakwa.kube nokukhumbula okumbalwa uma kuqhathaniswa nohlelo lapho ukuchayeka kwe-laser kwenziwa khona ngezikhathi zamaviki ama-3 (iqembu B).Kungakhathaliseki ukuthi iphrothokholi yefrikhwensi evamile7,13,26 noma iphrothokholi yamaviki angu-315,17,18, zombili lezi zivumelwano zichazwe ezincwadini.Ngokusekelwe emiphumeleni evezwe ocwaningweni lwamanje, i-null hypothesis ayizange yenqatshwe, futhi ngokusetshenziswa kwezivumelwano ezimbili ezifundwayo, izinombolo ezilinganayo zokunyakaza kwezinja zafinyelelwa.
Idizayini yocwaningo yamanje iyisivivinyo somtholampilo esilawulwa ngokungahleliwe (RCT).Ama-RCT athathwa njengezinga legolide lokuhlola imiphumela yokungenelela27.Inqubo yokuhlukaniswa komlomo nayo yasetshenziswa, inzuzo eyinhloko ewukuthi ukuhlukahluka phakathi kwezihloko kuyaqedwa, isiguli ngasinye sisebenza njengomlawuli waso, ngaleyo ndlela kunciphisa inani labahlanganyeli elidingekayo.
Zonke izifundo ezifakwe ocwaningweni zidinga ukukhishwa kwe-maxillary yokuqala ye-premolar elandelwa ukuhoxiswa kwe-canine njengengxenye yokwelashwa kwe-orthodontic.Njengoba ukukhishwa kungashintsha izinga le-RTM ngokwandisa umsebenzi wezimpawu zokuvuvukala, okungase kufihle umphumela we-LILT futhi kunikeze ukufundwa kwamanga kwamazinga e-IL-1β lapho usebenzisa i-laser, ukwelashwa kokukhipha kwenziwa ngaphambi kokwelashwa, okunikeze umphumela omuhle.Isixazululo sokukhipha isokhethi sokuphulukisa sinikeza isikhathi esanele futhi sinqoba imiphumela yezenzakalo zokusheshisa kwesifunda28.Lesi sinyathelo sokuqapha siphinde sathathwa abanye ababhali, i-11 ephenye umphumela we-LILT ngesilinganiso se-OTM ngesikhathi sokuhoxiswa ezinjeni ngokulinganisa amazinga we-biomarker afana ne-IL-1β nokuguqula ukukhula kwe-β1 (TGF-β1) ku-GCF.
Uhlobo lwe-laser olusetshenziswe kulolu cwaningo kwakuyi-laser ye-diode semiconductor esetshenziswa ku-980 nm ngokuya ngezincomo zomenzi ze-biostimulation efanele.Lokhu kungachazwa yiqiniso lokuthi uma ubude begagasi be-laser (650-1200 nm) buyisikhathi eside, izicubu zingena ngokujula29.Kodwa-ke, le wavelength enconyiwe isetshenziswe kwezinye izifundo ezimbalwa, ikhiqiza imiphumela emihle yokusheshisa ye-8.30 kanye nemiphumela emibi ye-14.
Esinye isici esibalulekile esinomthelela ekusebenzeni kokwelashwa kwe-LILI kanye ne-biostimulation umthamo noma ukuminyana kwamandla.Lapho kubuyekezwa izincwadi, kwatholakala ukuthi kunokuhlukahluka okukhulu kumthamo we-LILI energy ukusheshisa i-GTM.Abanye ababhali babika imiphumela emihle lapho besebenzisa ukuminyana kwamandla aphansi kusuka ku-0.7131, 532.33, 7.514 kuya ku-8 J/cm234.35, kuyilapho abanye abacwaningi babika umphumela we-LILR esilinganisweni se-GTM ngokuminyana kwamandla aphezulu, isibonelo, 25 J/cm2.cm27.36.Emsebenzini wamanje, umthamo wezinga eliphansi le-laser energy engu-8 J/cm2 ulethwe ukuchayeka okukodwa kumpande we-maxillary canine imizuzwana engu-8 kusetshenziswa ithiphu eliphezulu eliyisicaba ukuze kusatshalaliswe indawo ye-beam engu-1 cm2.Kukhona ukuhlobana okuqondile phakathi kosayizi we-beam nokujula kokungena kwe-laser, okubuye kuthethelele ukusetshenziswa kweziqeshana zezandla eziphezulu eziyisicaba kulolu cwaningo29,37.Iphrothokholi eyodwa yohlelo lokusebenza enosayizi omkhulu wendawo ye-beam yenziwa ngokuqondanisa nokuqondanisa 8 kanye nokuhlehla kwe-canine 38.
I-IL-1β iyaziwa njenge-cytokine ebalulekile ye-pro-inflammatory ekuqaleni kwe-OTM futhi ibhekwa njengophawu lwe-bone resorption.Ngakho-ke, amazinga e-IL-1β ahlolwe nge-laser ezifundweni eziningana11,39,40 emzamweni wokunquma ukuhlobana kwawo.Ocwaningweni lwamanje, amazinga e-IL-1β ku-GCF ahlolwe ezinhlangothini zokuhlola nokulawula zeqembu ngalinye ngokusebenzisa izinhlobo ezimbili ezihlukene ze-LILI ngezinsuku ezingu-0, 7, 14, no-21.
Esifundweni samanje, ukuhoxiswa kwe-canine nge-laser emaqenjini A no-B kwakuphakeme kakhulu kuneqembu lokulawula kuzo zonke izikhathi amaphuzu ahlolwe, ukuphakama kweviki 3, kwehla isonto elilodwa ngeviki le-6, bese kancane kancane kwanda kuze kube yisonto le-12..Ukunyakaza kwe-canine ephakeme okuphawulwe ngeviki le-3 kungachazwa ngomphumela wokufuduka kwamazinyo okuqala, okuhlanganisa: ukufuduka kwezimpande ku-PDL, ukukhubazeka kwamathambo ngenxa yokuguquguquka nokukhasa, nokucindezela kokucindezela kwezinyo ngenxa yokuthambekela kwe-conical socket Plane effect 41. Ngaphezu kwalokho, kutholakale ukuthi zonke izinqubo zezinto eziphilayo ezisebenzayo zihlala zihamba ngokushesha lapho i-deform ishintshile.Ukwehla okulandelayo okubonwe phakathi kwe-3 ne-6 amasonto, okungenzeka ngenxa yesikhathi sokulibaziseka esingahluka kusuka ku-2 kuya kumaviki angu-10, yisikhathi sokuphazamiseka kwe-PDL evuselela futhi isuse ithambo eliseduze nendawo yokuchoboza, okuvumela ukunyakaza kwamathambo.amazinyo.Esinye isici esinomthelela kulokhu kubheka kungase kube ukuthi imicu ye-oxygenated, i-collagen fibers, nokulungiswa kabusha kwamathambo e-alveolar ohlangothini lokungezwani kungase futhi kunciphise izinga lokunyakaza kwamazinyo.Amaphethini afanayo okunyakaza kwamazinyo atholakala esifundweni se-cleft45 ngokuqhathanisa imiphumela ye-LILI ne-corticotomy ngezinga lokuhlehliswa kwe-canine, baphawula ukuthi ukunyakaza kwamazinyo kwakukhulu kakhulu emasontweni 2 no-5, kulandelwa ukwehla okubukhali kumaviki angu-2 no-5.isonto th.Lokhu akuzange kubikwe ngasohlangothini lwe-laser ngeviki lesi-7, kodwa hhayi ngasohlangothini lwe-corticotomy.
Iphesenti elisho ukwanda kwephesenti ebangeni lokunyakaza kwe-maxillary canine ukusuka ohlangothini lwe-laser lalingu-40.78% eqenjini A kanye no-40.22% eqenjini B. Ukwanda okubonakalayo kokuhamba kwamazinyo okuhambisana nokusetshenziswa kwe-laser kungachazwa ezingeni lamaselula ngokumuncwa kwamandla e-laser ngama-photoreceptors ku-chainrial-transport membrane.Lo mphumela uholela ekusebenzeni kwesikhashana kweketanga lokuphefumula, okuholela ku-phosphorylation ye-oxidative kanye nezinguquko esimweni se-redox se-mitochondria yeselula ne-cytoplasm.Ngokulandelayo, amandla okushayela eseli ayanda ngokukhuphula ukuhlinzekwa kwe-ATP.Ngaphezu kwalokho, kukhona ukwanda kwamandla e-membrane ye-mitochondrial, i-alkalization ye-cytoplasm, nokuhlanganiswa kwama-nucleic acid.Njengoba i-ATP yaziwa njengohlobo lwemali yamandla amaseli, i-LILI inomthelela ekusebenzeni okuvamile kwamaseli ngokwakha indawo ekahle yokunyakaza kwamazinyo46.Ngakho-ke, kusukela emiphumeleni yethu, singaphetha ngokuthi ukusetshenziswa kwe-LILT njenge-adjunct ekwelapheni kwamathambo kungasheshisa ngempumelelo i-OTM kungakhathaliseki ukuthi isetshenziswa kaningi njengohlelo lweqembu A (ngezinsuku 0, 3, 7, 14 futhi nsuku zonke).ngemva kwezinsuku ezingu-2) amasonto), noma uma isetshenziswa izikhathi ezimbalwa eqenjini B (njalo emavikini angu-3), ngakho-ke, i-null hypothesis ayizange yenqatshwe.
Imiphumela efanayo yokusheshisa efanayo yezivumelwano ezimbili ezihloliwe ze-LILT ezibikwe kulolu cwaningo ingase ibe ngenxa yokuba khona komkhawulo wokwenza kusebenze amaselula lapho ukukhuphuka kokusebenza kwamaselula nokuchayeka kwe-LILT kwenzeka ekuqaleni, kodwa bese kuba ukuchayeka okuphindaphindiwe (njengakuqembu A), ngenxa yokusabela okugcwele kwebhayoloji ngeke kuholele ekuqhubekeni kusebenze.Ngakho-ke, singacabanga ukuthi imiphumela ye-LLLT ezingeni lamaselula ayikwazi ukunqwabelana.Mayelana nobudlelwano phakathi kwezinga lamandla kanye nesivinini sokunyakaza kwamazinyo, umqondo we-biosaturation uchazwe ngaphambilini.
Ngemva kokubuyekeza izincwadi ezikhona, siqhathanise ukwanda kwe-1.4-fold (40-41%) ku-WTM etholwe ocwaningweni lwethu kusetshenziswa izivumelwano ezimbili ze-laser nemiphumela yeminye imibiko eminingana.Ezinye izifundo zibike imiphumela efanayo11,30,48,49 kuyilapho ezinye zibike amanani aphansi kancane wokusheshisa asetshenziswe kusetshenziswa i-LILI7,18,32,40.Ngakolunye uhlangothi, amanani okusheshisa aphezulu kakhulu kunalawo abikwe ezivivinyweni zamanje, kusukela ku-1.65×17 kuya cishe ku-2x OTM15, 34, 39, 50, okungenzeka ahlobene namanye awo Sebenzisa ama-braces azimele ngaphandle kokungqubuzana 15. Lo mehluko emiphumeleni eshicilelwe ezincwadini kungase kube ngenxa yokukhishwa kwe-waves, amaphethini e-exposure yesikhathi sokwelashwa, amaphethini we-waveleng exposure, amaphethini we-waveleng we-laser, njll. ., okwenza ukuqhathanisa okuqondile phakathi kwezifundo ezahlukene kube nzima kakhulu..Kodwa-ke, kuye kwaphawulwa ukuthi ukuminyana kwamandla okuphansi (isb. 2.5, 5 kanye no-8 J/cm2) kunikeza ukusebenza kahle kokusheshisa uma kuqhathaniswa nokuminyana kwamandla okuphezulu, kubalulekile ukuqaphela ukuthi imithamo esetshenziswe ekuhloleni kwethu yayingu-8 J/cm2.cm2.
Ukuhunyushwa kwamazinga e-IL-1β ku-distal cleft (uhlangothi lokucindezela) ngemva kokuhlaziywa kwamasampula e-GCF atholiwe kubonise ukukhuphuka okuphawulekayo kwezibalo kusukela kwesisekelo (okungukuthi ukuphakama) ngosuku lwe-7 okulandelwa ukwehla kancane kancane kuya kwesisekelo.kumaphaneli A no-B, ohlangothini lwe-laser nasohlangothini lokulawula.Lokhu kungachazwa yiqiniso lokuthi isigaba sokuqala se-OTM sivame ukuhambisana nokwanda komsebenzi we-osteoclast.I-IL-1β iphinde ibhekwe njengophawu lokuqala olutholakalayo oluhlotshaniswa ne-bone resorption, futhi inkulumo ye-IL-1β kuye kwabikwa ukuthi yanda ngamandla futhi kamuva yehla ezifundweni eziningi11,20,51.
Ukwengeza, amazinga e-IL-1β ayephezulu ohlangothini lwe-laser uma kuqhathaniswa neqembu lokulawula kuwo womabili amaqembu ocwaningo kuwo wonke amaphuzu wesikhathi esilinganisiwe ngaphandle kwesisekelo sokuqala, futhi kwakukhona umehluko ophawulekayo phakathi kwabo.Lokhu kukhombisa ukuthi ukukhanya kwe-laser okunamandla aphansi kubangele ukusabela okuthuthukisiwe kwebhayoloji ezicutshini ze-periodontal ohlangothini lokuhlola ngendlela yokuvuselela ukusebenza kwe-osteoclast ohlangothini olucindezelwe ngesikhathi sokunyakaza kwamazinyo.Lo mphumela we-LLLT kumazinga e-IL-1β uboniswe ezifundweni ezihlukahlukene11,39,40.
Uma kuqhathaniswa namazinga e-laser-side IL-1β emaqenjini amabili ocwaningo, amazinga ayephakeme ngokwezibalo eqenjini A uma kuqhathaniswa neqembu B ezinsukwini ezingu-7 no-14. Lokhu kungachazwa ngenani elikhulu lokuchayeka kwe-laser irradiation eqenjini A phakathi nenkathi yokubuka kwezinsuku ezingu-21, lapho i-irradiation yenziwa khona ngezinsuku ze-0, 3, 7, futhi isibhamu se-1, no-1, no-1 kuphela, no-1, IL, no-14, no-1, IL kuphela Amazinga ka-β ayephezulu ngokwezibalo ohlangothini lwe-laser eqenjini A, lo mehluko wezibalo awuzange uboniswe ngokomtholampilo ngezinga lokuhlehla ezinjeni uma kuqhathaniswa nohlangothi lwe-laser eqenjini B, njengoba bekungekho ukubaluleka kwezibalo.Emaqenjini A no-B, umehluko obikiwe ekuhoxisweni kwe-canine phakathi kwezinhlangothi ze-laser empeleni kubangele inani elifanayo lokunyakaza kwe-canine.Ngakho-ke, singasho ukuthi umehluko wezibalo awuchazi ukubaluleka komtholampilo.
I-low-intensity laser therapy, lapho isetshenziswa nemingcele esetshenziswe kulolu cwaningo, ingasheshisa ngokuphumelelayo ukunyakaza kwamazinyo e-orthodontic cishe izikhathi ezingu-1.4, kungakhathaliseki ukuthi isetshenziswa ngemvamisa ephezulu noma ephansi, ehambisana nokulandelwa okuvamile, mhlawumbe.ifaneleka kakhulu iziguli.
Ukwanda kokuhamba kwamazinyo e-orthodontic ngesikhathi se-LILI kwakuhambisana nokwanda kwezinga le-interleukin-1β ohlangothini olucindezelwe, okubonisa ukuthi ukusetshenziswa kwe-LILI kubangela inqubo ethuthukisiwe yokuguqulwa kwamathambo.
Amasethi edatha asetshenzisiwe kanye/noma ahlaziywa ocwaningweni lwamanje ayatholakala kubabhali abafanele uma kunesicelo esifanele.
Skidmore, KJ, Brook, KJ, Thomson, WM & Harding, WJ Factors ezithonya isikhathi sokwelashwa ezigulini zamathambo. Skidmore, KJ, Brook, KJ, Thomson, WM & Harding, WJ Factors ezithonya isikhathi sokwelashwa ezigulini zamathambo.Skidmore, KJ, Brook, KJ, Thomson, WM and Harding, WJ Factors ezithinta isikhathi sokwelashwa ezigulini zamathambo. Skidmore, KJ, Brook, KJ, Thomson, WM & Harding, WJ 影响正畸患者治疗时间的因素. Skidmore, KJ, Brook, KJ, Thomson, WM & Harding, WJSkidmore, KJ, Brook, KJ, Thomson, WM and Harding, WJ Factors ezithinta isikhathi sokwelashwa seziguli zamathambo.Yebo.G. Isonto Lobu-Orthodox.I-Orthodontics.129, 230-238.https://doi.org/10.1016/j.ajodo.2005.10.003 (2006).
I-Kurol, J., Owman-Moll, P. & Lundgren, D. I-resorption yezimpande ehlobene nesikhathi ngemva kokusetshenziswa kwamandla aqhubekayo alawulwayo wama-orthodontic. I-Kurol, J., Owman-Moll, P. & Lundgren, D. I-resorption yezimpande ehlobene nesikhathi ngemva kokusetshenziswa kwamandla aqhubekayo alawulwayo wama-orthodontic.I-Kurol, J., Ouman-Moll, P., kanye ne-Lundgren, D. Ukufakwa kabusha kwempande okuhlobene nesikhathi ngemva kokusetshenziswa kwamandla alawulwayo wama-orthodontic. Kurol, J., Owman-Moll, P. & Lundgren, D. 施加受控连续正畸力后与时间相关的牙根吸收. Kurol, J., Owman-Moll, P. & Lundgren, D.I-Kurol J, Ouman-Moll P, kanye no-Lundgren D. Ukufakwa kabusha kwezimpande okuncike esikhathini ngemva kokusetshenziswa kwamandla alawulwayo avamile we-orthodontic.Yebo.G. Isonto Lobu-Orthodox.I-Orthodontics.110, 303–310.https://doi.org/10.1016/s0889-5406(96)80015-1 (1996).