Ua tsaug rau koj tuaj xyuas Nature.com.Qhov browser version koj siv tau txwv CSS kev txhawb nqa.Rau qhov kev paub zoo tshaj plaws, peb xav kom koj siv qhov browser tshiab (lossis lov tes taw Compatibility Hom hauv Internet Explorer).Nyob rau lub sijhawm no, txhawm rau ua kom muaj kev txhawb nqa txuas ntxiv, peb yuav ua rau lub xaib tsis muaj qauv thiab JavaScript.
Lub hom phiaj ntawm txoj kev tshawb no yog los soj ntsuam cov canine retraction tus nqi rau ob qhov kev siv hluav taws xob qis qis qis (LLLT) irradiation regimens, nrog rau siab thiab qis zaus.Nees nkaum tus neeg mob tau muab faib ua ob pawg.Hauv pawg A, ib sab ntawm maxillary koov tau randomized kom tau txais LILT rau hnub 0, 3, 7, 14, thiab txhua 2 lub lis piam tom qab ntawd, thaum nyob hauv pawg B, ib sab tau txais LILT txhua 3 lub lis piam.Thaum lub sijhawm kawm 12-lub lim tiam, cov hniav txav tau raug kuaj xyuas txhua peb lub lis piam txij thaum pib ntawm canine retraction.Tsis tas li ntawd, cov qib ntawm interleukin-1β (IL-1β) hauv cov kua dej gingival sulcus tau soj ntsuam. Cov txiaj ntsig tau nthuav tawm qhov nce ntxiv ntawm cov canine retraction tus nqi ntawm lub laser sab ntawm pawg A thiab B, piv nrog cov tswj sab (p <0.05), tsis muaj qhov sib txawv tseem ceeb ntawm cov laser sab hauv ob pawg (p = 0.08–0.55). Cov txiaj ntsig tau nthuav tawm qhov nce ntxiv ntawm cov canine retraction tus nqi ntawm cov laser sab ntawm pawg A thiab B, piv nrog cov tswj sab (p <0.05), tsis muaj qhov sib txawv tseem ceeb ntawm cov laser sab hauv ob pawg (p = 0.08–0.55). Результаты выявили значительное увеличение скорости ретракции клыков на стороне лазера в груплькни A ой стороной (p < 0,05), без существенных различий между сторонами лазера в обеих группах (p = 0,58). Cov txiaj ntsig tau nthuav tawm qhov kev nce siab ntawm canine retraction ceev ntawm lub laser sab hauv pawg A thiab B piv rau sab tswj (p <0.05), tsis muaj qhov sib txawv ntawm cov laser sab hauv ob pawg (p = 0.08–0.55). ).结果显示，与对照组相比，A 组和B 组激光侧的犬齿回缩率显着增加 (p <0.05)着差异(p = 0.08-0.55).结果显示 , 与 对照组 , 组和 a 组和 b 组 激光侧 犬齿回 缩率 显牀 (p <0.05)间 显着 差异 (p = 0.08-0.55 . . . . . . . Результаты показали, что по сравнению с контрольной группой скорость ретракции кплыков на стороне ла стороне ла значительно выше (p < 0,05), а на стороне лазера не было существенной разницы между двумя групп5,5,8 (p = 0,0. Cov txiaj ntsig tau pom tias, piv nrog rau pawg tswj hwm, cov canine retraction tus nqi ntawm lub laser sab hauv pawg A thiab B tau nce siab dua (p <0.05), thiab tsis muaj qhov sib txawv ntawm ob pawg ntawm laser sab (p = 0.08-0.55). Tsis tas li ntawd, qib IL-1β tau nce siab dua ntawm cov laser sab ntawm ob pawg, piv nrog cov tswj sab (p <0.05). Tsis tas li ntawd, qib IL-1β tau nce siab dua ntawm cov laser sab ntawm ob pawg, piv nrog cov tswj sab (p <0.05). Кроме того, уровни IL-1β были значительно выше на стороне лазера в обеих группах по сравнению с контронер 5 Tsis tas li ntawd, qib IL-1β tau nce siab dua ntawm laser sab hauv ob pawg piv rau sab tswj (p <0.05).此外，与对照组相比，两组激光侧的IL-1β 水平显着升高 (p <0.05).此外，与对照组相比，两组激光侧的IL-1β 水平显着升高 (p <0.05). Кроме того, уровни ilоли золи золи полоно павнера по гокра правнениюй грольнтой (P <0 , 05). Tsis tas li ntawd, qib IL-1β tau nce siab ntawm lub laser sab hauv ob pawg piv rau pawg tswj hwm (p <0.05).Yog li, LILI muaj peev xwm ua kom cov hniav txav tau zoo, txawm tias nws tau siv ntau zaus lossis tsis tshua muaj, uas tau cuam tshuam nrog cov lus teb lom neeg ntau ntxiv, uas tau cuam tshuam txog qib ntawm IL-1β nce ntawm sab compressed.
Kev kho hniav mus sij hawm ntev (feem ntau nyob ib ncig ntawm 20-30 lub hlis 1) tau pom tias muaj kev cuam tshuam tsis zoo rau cov neeg mob ua raws, ntxiv rau cov kev pheej hmoo xws li hauv paus resorption2, caries3, enamel decalcification3 thiab cov teeb meem periodontal4,5.Yog li ntawd, ntau txoj hauv kev tau raug npaj los txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhawm rau txhuam hniav (OTM)Tsis tas li ntawd, cov txiaj ntsig ntawm kev sib txuas ob txoj hauv kev nrawm thiab cov txiaj ntsig ntawm kev rov ua cov txheej txheem kev nrawm ntawm qhov nrawm ntawm OTM6 tau tshawb xyuas.
Tsawg siv laser therapy (LLLT) tau yog ib qho ntawm cov txheej txheem uas tsis yog kev phais kom ceev OTM, tab sis tau muaj kev tsis sib haum xeeb hauv cov lus ceeb toom ntawm nws cov txiaj ntsig hauv cheeb tsam no, thaum cov txiaj ntsig zoo 7,8 thiab qhov tsis zoo tau raug sau tseg.Cov txiaj ntsig tsis sib haum no tuaj yeem piav qhia los ntawm qhov sib txawv ntawm cov ntawv thov laser siv nyob rau hauv txhua txoj kev tshawb fawb, suav nrog hom laser, hom kev thov, wavelength, radiation koob tshuaj, thiab lub sij hawm raug, txij li cov tsis muaj feem cuam tshuam ncaj qha rau cov txiaj ntsig ntawm laser daim ntawv thov 10.
Nyob rau hauv cov nqe lus ntawm daim ntawv thov txoj kev, ntau yam laser irradiation raws tu qauv tau raug tshaj tawm los pab cov hniav txav.Ib txoj kev siv dav dav suav nrog kev siv lub laser rau hnub 0, 3, 7, 14, 21 thiab 30, rov ua tib yam li txhua lub hlis, thiab cov txheej txheem no tau txais los ntawm ntau tus kws sau ntawv 11,12.Lwm tus tau siv lwm txoj kev tswj hwm uas zoo sib xws rau cov txheej txheem uas tau piav qhia dhau los thiab tseem yog ib txoj hauv kev siv dav dav, uas LILI tau siv rau hnub 0, 3, 7, 14, thiab tom qab ntawd txhua 15 hnub kom txog thaum kawg ntawm lub sijhawm kawm.13. Tsis tas li ntawd, ib txoj cai tau npaj tseg uas suav nrog txhua lub lim tiam ntawm kev siv lub laser siv tsawg thoob plaws lub sijhawm canine retraction.Txawm li cas los xij, qhov tsis zoo ntawm cov txheej txheem no yog qhov siab ntawm cov neeg mob cov lus pom zoo, uas tuaj yeem ua rau tsis yooj yim rau txhua tus.Yog li, cov txheej txheem uas yuav tsum tau siv cov neeg mob tsawg dua, piv txwv li, suav nrog LILI 8 zaug hauv ib hlis lossis 15, 16, 17, 18 txhua 3 lub lis piam.
Txij li thaum orthodontic rog paub tias yuav ua rau cov pob txha hloov kho, kev txhim kho ntawm cov kev hloov inflammatory yog qhov yuav tsum tau ua ua ntej rau cov txheej txheem no, ua rau misalignment ntawm cov hniav 19.Raws li ntau txoj kev tshawb fawb, ib txoj hauv kev los ntsuam xyuas cov xwm txheej lom neeg hauv lub cev ligament yog los ntsuas qib ntawm cytokines hauv cov kua dej gingival sulcus (GCF).Interleukin-1β (IL-1β) yog ib qho cytokine nquag heev hauv cov pob txha metabolism thiab suav hais tias yog ib qho ntawm cov cytokines muaj zog tshaj plaws hauv OTM cov ntaub so ntswg thaum ntxov.Txij li thaum muaj kev sib raug zoo ntawm IL-1β theem thiab kev ciaj sia, fusion thiab osteoclast ua kom, IL-1β tuaj yeem suav tias yog ib qho tseem ceeb rau kev suav cov qib ntawm cov hniav orthodontic, uas cuam tshuam rau kev ua haujlwm ntawm cov pob txha alveolar remodeling24.
Yog li, lub hom phiaj ntawm peb txoj kev tshawb fawb yog los soj ntsuam thiab sib piv cov txiaj ntsig ntawm NILT nrog cov kev siv ntau zaus, suav nrog kev siv ntau zaus ntawm hnub 0, 3, 7, 14, thiab tom qab ntawd txhua 2 lub lis piam piv nrog kev siv txhua 3 lub lis piam.Retraction tus nqi nyob rau hauv dev nyob rau hauv ib qho kev sim los txo qhov zaus ntawm tus neeg mob rov qab.Tsis tas li ntawd, IL-1β theem hauv GCF tau soj ntsuam siv ob txoj cai.Lub null hypothesis ntawm txoj kev tshawb fawb tam sim no yog tias tsis muaj qhov sib txawv ntawm qhov tshwm sim ntawm canine retraction nrog LILI siv ob txoj kev xeem.
Txoj kev tshawb no yog ib qho kev sim tshuaj ntsuam xyuas randomized nrog ob pawg sib npaug, txhua qhov kev sim LILI raws tu qauv.Txhua pab pawg tau txais kev sib cais lub qhov ncauj tsim, ib sab yog pawg tswj hwm thiab lwm qhov yog pab pawg kawm.
Txoj kev tshawb no suav nrog 20 tus neeg mob hnub nyoog 15 txog 20 xyoo uas xav tau kev kho mob tshem tawm thawj zaug premolars ntawm lub puab tsaig sab sauv, ua raws li kev tshem tawm ntawm cov canines.Cov piv txwv loj xam tau raws li qhov yuam kev alpha ntawm 5% thiab kawm lub zog ntawm 80%.Qhov kev suav no yog nyob ntawm qhov nruab nrab thiab tus qauv sib txawv ntawm canine retraction hauv kev tshawb fawb uas Doshi-Mehta thiab Bhad-Patil7 tau thov LILI hnub 0, 3, 7, 14 thiab txhua 2 lub lis piam tom qab ntawd (Arm A) thiab hauv Qamruddin kev tshawb fawb li al.Lwm qhov hauv 15 qhov kev tshawb fawb, LILI tau thov txhua 3 lub lis piam (paub B).Kev pom zoo rau kev ncaj ncees tau txais los ntawm Ethics Council ntawm Kws Kho Hniav, Alexandria University, Alexandria, Egypt (IRB: 00010556-IORG: 0008839).Tus lej ntawm Pawg Neeg Saib Xyuas Kev Ncaj Ncees yog 0111-01/2020.Pom zoo rau Lub Ib Hlis 21, 2020. Qhov kev sim no tau sau npe nrog ClinicalTrials.gov ua "Ob theem qis Laser Cov Txheej Txheem txhawm rau ntsuas qhov nrawm nrawm hauv dev."Tus lej sau npe yog NCT04926389.Hnub mus sib hais yog 06/15/2021 ntawm https://clinicaltrials.gov/ct2/show/NCT04926389.Kev tso npe ntawm cov neeg mob hauv txoj kev tshawb fawb tau pib thaum Lub Ob Hlis 5, 2020 thiab xaus rau lub Kaum Ib Hlis 28, 2021.
Cov neeg mob tau raug xaiv los ntawm lub tsev kho mob orthodontic ntawm Kws Kho Hniav ntawm Alexandria University.Cov kev kawm tau raug tshuaj xyuas thiab tshuaj xyuas raws li cov hauv qab no: kev noj qab haus huv, tsis muaj kab mob, tsis muaj kev kho hniav yav dhau los, kev tu lub qhov ncauj kom txaus, thiab cov ntaub so ntswg noj qab nyob zoo.Cov neeg mob koom nrog thiab lawv niam lawv txiv tau muab cov lus piav qhia tag nrho thiab nthuav dav ntawm cov txheej txheem kev kawm, thiab yog li ntawd, tau txais kev pom zoo los ntawm txhua qhov kev kawm.Tag nrho cov txheej txheem tshawb fawb tau ua raws li cov lus qhia thiab cov cai uas tau teev tseg hauv Kev Tshaj Tawm ntawm Helsinki.
Ua ntej pib canine retraction, 20 tus neeg mob tau raug xaiv thiab random muab rau pawg A lossis pawg B (10 hauv txhua pab pawg) rau kev siv laser siv tsawg.Randomization tau ua tiav siv cov txheej txheem randomization yooj yim nrog qhov sib faib ntawm 1: 1.Ib lub thawv tau npaj muaj nees nkaum daim ntawv tais, kaum ntawm cov ntawv sau nrog lo lus "Pab A" thiab lwm kaum nrog lo lus "Pab B".Txhua tus neeg koom nrog raug nug kom xaiv ib daim ntawv tais diav los ntawm ib lub thawv thiab muab rau ib qho ntawm ob pawg raws li.Cov txheej txheem tib yam tau rov ua dua nyob rau hauv txhua pab pawg, xaiv ib sab ntawm maxillary arch li "test" thiab sab nraud li "tswj" hauv kev sib cais-qhov ncauj.
Ntxiv nrog rau cov ntaub ntawv kho hniav ib txwm muaj (cov duab sab hauv thiab sab nrauv, cov duab hluav taws xob, thiab cov kev xav kho hniav), cov ntsiab lus npaj rau kev kho hniav kho hniav tau sau npe los ntawm kev sau lawv cov keeb kwm kho mob thiab kho hniav.Cov neeg mob kuj tau hais kom ua tiav qhov ncauj ntxuav thiab polishing ua raws li cov lus qhia hauv kev tu lub qhov ncauj (siv txhuam hniav, txhuam hniav thiab txhuam hniav).
Maxillary thiab mandibular fixation nrog ncaj hlau Roth khoom siv (Mini 2000; Ormco, USA) nrog 0.022 "\(\x) 0.028" slots tau kho nyob rau hauv tag nrho cov neeg mob recruited, qhov uas cov txheej txheem fixation yog standardized rau ob pawg thiab txiav txim los ntawm tib tus neeg teb xov tooj..Tom qab ntawd, tus neeg mob raug xa mus rau kev rho tawm ntawm maxillary thawj premolar kom tso cai rau lub sijhawm txaus rau lub qhov (socket) kho tom qab rho tawm ua ntej pib rho tawm kwv yees li 2 lub hlis tom qab rho tawm.Kev sib tw pib thiab kev sib tw ua tiav thaum 0.016 "x 0.022" stainless hlau xaim tuaj yeem nkag mus rau hauv txhua cov hniav maxillary.
Ua ntej pib canine retraction, lub Upper ob premolars thiab thawj molars tau ligated ua ke nrog ib tug 0.009-nti daim duab-yim hlau nyob rau hauv kev sim thiab tswj sab ntawm ob pawg.Tsis tas li ntawd, cov maxillary incisors tau khi ua ke tib yam li cov ntu tom qab los pab kom ruaj khov thiab tiv thaiv lawv qhov kev sib cais.
Canine retraction nyob rau hauv pawg A thiab B tau ua los ntawm nickel-titanium (NiTi) kaw coil springs (Ormco, USA), ob qho tib si ntawm kev sim thiab tswj sab, ncab ntawm cov hooks ntawm canine brackets thiab cov hooks ntawm cov kwj dej molar, nrog lub zog ntawm 150 g yog ntsuas dynamometer (Morelli, Brazil).
Lub diode laser (Wiser; Tus Kws Kho Mob Smile-Lambda Spa, Brendol, Ltalis) tau siv los ua lub tshuab hluav taws xob qis, emitting infrared hluav taws xob nrog lub wavelength ntawm 980 nm thiab lub zog tso zis ntawm 100 mW nyob rau hauv tas li hom.Lub dav hlau nthwv dej fiber ntau (AB 2799; Tus Kws Kho Mob Smile-Lambda Spa, Brendola, Ltalis) tau siv los faib 1 cm2 nqaj qhov chaw nrog lub tiaj tiaj sab saum toj, muab cov lus qhia fiber ntau raws maxillary koov nyob rau hauv nruab nrab peb ntawm maxilla.canine cag nyob rau sab sim (raws li cov chaw tsim khoom cov lus qhia, qhov tsawg kawg nkaus ntawm 1.5 cm thaum tsis pom tseeb) rau 8 vib nas this (Fig. 1).Tag nrho lub zog ceev thov rau ib ntu yog 8 J / cm2 (1 J / cm2 ib ob).Lub laser tsis siv yog qhia nyob rau hauv Table 1. Ceev faj ua ntej siv lub laser, thiab ob tus neeg mob thiab tus neeg teb xov tooj siv tsom iav los ntawm cov chaw tsim khoom, nyob ntawm seb lub wavelength siv.
Cov lus qhia fiber ntau tau tuav ntawm qhov deb ntawm 1.5 cm los ntawm lub hauv paus ntawm maxillary canine ntawm kev sim sab raws li cov chaw tsim khoom cov lus qhia.
Cov txheej txheem cais lub qhov ncauj tau siv nyob rau hauv ob pawg, thiab txhua tus neeg koom nrog tau txais LILI ntawm ib sab ntawm maxillary koov thiab sab nraud raws li kev tswj hwm.Hauv pawg A, cov kev kawm tau txais LILT rau hnub 0, 3, 7, 14 thiab tom qab ntawd txhua 2 lub lis piam, thaum nyob hauv pawg B nws tau siv txhua 3 lub lis piam ntawm kev sim rau tag nrho lub sijhawm kawm (12 lub lis piam) ntawm LILT.Lub laser beam kuj tau kho tas li ntawm kev tswj xyuas ntawm ob pawg, muab cov txiaj ntsig placebo ua ib feem ntawm cov txheej txheem ntawm cov neeg mob dig muag.Vim qhov xwm txheej ntawm kev cuam tshuam ntawm theem no, tus neeg teb xov tooj tsis tuaj yeem dag.
Ua ntej sau cov qauv, ob sab ntawm cov maxillary canines tau ntxuav nrog paj rwb swabs, cais nrog tus kheej txhawb nqa retractors, suction, thiab paj rwb yob, thiab maj mam cua qhuav rau 5 s.Cov qauv raug coj los ntawm qhov chaw nruab nrab ntawm cov maxillary canines siv cov qauv lim ntawv strips (Whatman, Maidstone, UK) thiab txiav rau hauv qhov ntau thiab tsawg ntawm 2 × 10 mm2.Maj mam ntxig txhua txoj hlua rau hauv qhov sib txawv kom txog thaum koj hnov ​​​​qhov tsis kam me ntsis, tom qab ntawd tso rau hauv qhov chaw rau 60 vib nas this thaum tswj kev sib khi kom zoo (Daim duab 2).Tom qab tshem tawm, cov strips tshiab tau muab tso rau txhua 1 min kom tau 4 strips ntawm txhua qhov chaw.Kev ntsuas kuj tau coj los ua kom tsis txhob muaj kev puas tsuaj rau cov gingival fissure.Pov tseg cov qauv uas muaj cov qaub ncaug lossis ntshav thiab khaws cov qauv tshiab.GCF cov qauv raug coj mus rau hauv lub hauv paus (ua ntej qhov pib ntawm canine retraction), los ntawm qhov distal canine fissures nyob rau hauv pawg A thiab B, ntawm kev sim thiab tswj sab, tshwj tsis yog rau hnub 7, 14, thiab 21.
Alginate impressions (Ca37; Cavex, Haarlem, Lub Netherlands) tau ua ua ntej canine retraction thiab tau rov ua dua txhua 3 lub lis piam nyob rau hauv 12-lub lim tiam kawm ntawm txhua txhua mus ntsib.Nyob rau txhua qhov kev mus ntsib, cov hlau thiab coil springs raug tshem tawm, qhov kev xav alginate raug coj mus, thiab cov lej raug muab pov tseg.Tom qab ntawd tus qauv hniav raug txiav thiab cim nrog tus neeg mob lub npe, tus lej thiab hnub tim.Cov qauv plaster tau raug kuaj xyuas (inEos X5 CAD/CAM kuaj kuaj; Dentsply Sirona, PA, USA) los tsim peb-dimensional (3D) cov duab ntawm tus qauv hniav.Cov kev ntsuas tsim nyog tau ua los ntawm AutoCAD version 2013 (AutoCAD; Autodesk, USA).Cov kws kho mob tsis paub txog qhov kev sim thiab kev tswj xyuas sab hauv lub sijhawm ntsuas kom tsis txhob muaj qhov tsis ncaj ncees lawm, thiab kev soj ntsuam kev ntseeg siab tau ua nrog kev ntsuas rov qab los ntawm tib tus neeg teb xov tooj ib lub lis piam tom qab kuaj xyuas qhov ntsuas qhov yuam kev.Qhov ntsuas ntsuas qhov yuam kev yog 6%.
Ntau qhov chaw pom tau pom nyob rau ntawm cov hniav cam, nrog rau qhov nruab nrab palatal suture, cov ntsiab lus nruab nrab ntawm sab laug thiab sab xis thib peb, thiab cusps ntawm sab laug thiab sab xis maxillary canines.Txoj kab ntsug khiav los ntawm cov ntsiab lus nruab nrab ntawm sab laug thiab sab xis thib peb folds thiab tubercles ntawm sab laug thiab sab xis maxillary canines mus rau qhov nruab nrab palatine suture.Kev ntsuas anterior-posterior raug coj los ntawm ob sab canine kab thiab kab thib peb los ntsuas canine retraction (Fig. 3, 4).
Nrhiav thaj chaw ntawm cov duab scanned ntawm cov qauv hniav los ntsuas canine retraction.(Ib tug).Nruab nrab palatal suture.(b, d).Tubercles ntawm sab laug thiab sab xis maxillary canines, feem.(c, e).Cov kab sib txuas mus rau sab hauv kawg ntawm peb sab laug thiab sab xis folds, feem.
Tom qab tshem tawm ntawm cov gingival crevice, pawg ntawm plaub lim ntawv strips sau rau hauv ib qho chaw tau muab tso rau hauv Eppendorf hlab (Capp, Denmark) uas muaj 100 µl ntawm phosphate-buffered saline.Eppendorf cov raj tau muab kaw thiab sau thiab cov qauv tau raug centrifuged tam sim ntawd ntawm 3000 rpm rau 10 min siv lub centrifuge (Hettich Universal 320R BC-HTX320; GMI, MN, USA) kom rov qab GCF cov qauv ntawm cov strips.Cov raj Eppendorf tau khaws cia ntawm -20 ° C kom txog thaum kuaj biochemical.Kev soj ntsuam ntawm qib IL-1β tau ua tiav siv qhov kev ntsuam xyuas enzyme-txuas immunosorbent (ELISA; Huab-Clone, Howe, USA).Qhov concentration ntawm IL-1β tau txiav txim los ntawm kev sib piv cov optical ceev (OD) ntawm cov qauv tau txais nrog tus qauv nkhaus thiab raws li xam qhov sib npaug ntawm linear regression ntawm tus qauv nkhaus.Thaum kawg, cov txiaj ntsig rau qib IL-1β tau nthuav tawm hauv pg / ml / 60 s25.Ib daim ntawv qhia txog kev kawm tsim muaj nyob hauv daim duab 5, uas qhia txog cov txheej txheem kawm.
Kev txheeb cais tau ua tiav siv IBM SPSS rau Windows version 23.0 (IBM; Armonk, NY, USA).Txhua qhov sib txawv ntawm qhov sib txawv tau ib txwm faib thiab txhais tau tias, tus qauv sib txawv (SD) thiab 95% kev ntseeg siab lub sijhawm (CI) tau suav thiab siv cov kev ntsuas parametric.Ntau qhov sib txawv (canine retraction thiab IL-1β theem) tau muab piv los ntawm ob pawg kev tshawb fawb siv cov qauv t-kev sim ywj pheej, thaum kev sib piv ntawm laser thiab tswj sab hauv txhua pab pawg tau ua nrog kev sib tw t-test.Dog retraction thiab IL-1β theem ntawm lub sijhawm sib txawv hauv txhua pab pawg tau muab piv rau nyias siv cov kev ntsuas rov ua dua ntawm qhov sib txawv ua raws los ntawm ntau qhov sib piv sib piv siv Bonferroni-kho qhov tseem ceeb theem. Qhov tseem ceeb tau teeb tsa ntawm tus nqi <0.05. Qhov tseem ceeb tau teeb tsa ntawm tus nqi <0.05. Значимость была установлена ​​при значении p <0,05. Qhov tseem ceeb tau teeb tsa ntawm tus nqi <0.05.显着性设定为p <0.05.显着性设定为p值 <0.05. Значимость была установлена ​​на уровне p <0,05. Qhov tseem ceeb tau teeb tsa ntawm p<0.05.
Thaum lub sijhawm kawm, tsis muaj ib qho kev kawm twg tau tso tawm thaum lub sijhawm ua ntej kev cuam tshuam lossis lub sijhawm tseem tshuav ntawm txoj kev kawm.Tag nrho 20 qhov kev xaiv thawj zaug tau ua tiav tag nrho 12 lub lis piam kawm (10 yam hauv ib pab).Tus neeg mob ntws rau tag nrho cov kev sim yog pom nyob rau hauv daim duab 6 siv CONSORT flowchart.Cov ntaub ntawv pej xeem rau cov kev kawm hauv Pawg A thiab B tau nthuav tawm hauv Table 2. Tsis muaj qhov tshwm sim ntawm kev tshwm sim hauv cov qauv kev kawm, uas tau ua txhua peb lub lis piam los ntsuas cov canine retraction.Tsis tas li ntawd, txhua qhov tau txais GCM cov qauv tau ua tib zoo ua tiav thiab tshuaj xyuas.
Tus nqi ntawm maxillary canine retraction ntawm lub sij hawm sib txawv cov ntsiab lus tau piav nyob rau hauv Table 3, hais txog ob pawg A thiab B. Nyob rau hauv pab pawg A, qhov loj tshaj qhov nruab nrab (± SD) mus los ntawm lub maxillary canine tau tshaj tawm nyob rau lub lim tiam thib 3 yog 1.18 (± 0.04) mm ntawm lub laser sab, thiab 0.804 qhov sib txawv ntawm mm stat (± 0) 0.001). Tus nqi ntawm maxillary canine retraction ntawm lub sij hawm sib txawv cov ntsiab lus tau piav nyob rau hauv Table 3, hais txog ob pawg A thiab B. Nyob rau hauv pab pawg A, qhov loj tshaj qhov nruab nrab (± SD) mus los ntawm lub maxillary canine tau tshaj tawm nyob rau lub lim tiam thib 3 yog 1.18 (± 0.04) mm ntawm lub laser sab, thiab 0.804 qhov sib txawv ntawm mm stat (± 0) 0.001). Величина ретракции верхнечелюстного клыка в разные моменты времени описана в таблице 3 для обеих г.В группе А наибольшее среднее расстояние (± SD), пройденное верхнечелюстным клыком на 3-й неделе, 1 ся4 те те 0 вм роне лазера и 0,85 (± 0,04) мм на стороне контроля, при этом разница между ними статистически значи0 ма (0,000). Tus nqi ntawm retraction ntawm maxillary canine ntawm lub sij hawm sib txawv cov ntsiab lus tau piav nyob rau hauv Table 3 rau ob pawg A thiab B. Nyob rau hauv pawg A, qhov ntev txhais tau tias nyob deb (± SD) mus los ntawm lub maxillary canine nyob rau lub lim tiam 3 yog 1.18 (± 0.04) mm ntawm lub laser sab thiab 0.85 (± 0.04) mm qhov sib txawv ntawm 0.85 (± 0.04) mm nyob rau hauv statically sib txawv ntawm lawv.Rau pawg A thiab B, qib ntawm maxillary canine retraction ntawm lub sijhawm sib txawv tau piav qhia hauv Table 3.在A 组中，上颌尖牙移动的最大平均距离(± SD) 在第3周报告为激光侧为1.18 (± 0.04) mm， 0.04 縧 ± 0.8 hli者之间的差异具有统计学意义 (p <0.001).在组中，上颌移动的最大距离距离距离在第 3周报告为激光侧 10姯 1 . 8 ± 1 .为 0.85 (± 0.04) mm ， 两 之间的 具有 具有 具有 具有 具有 具有 具有 具有 具有 具有 具有 具有 巅具有 具有 具有 具有 具有 具有 具有 具有 具有统计学意义（p < 0.001） . В группе А максимальное среднее расстояние (± SD) движения клыков верхней челюсти на 3-й неделе соротр 10 вим не лазера и 0,85 (± 0,04) мм на стороне контроля, разница между ними была Статистическая значимость,0 (p <1). Hauv pab pawg A, qhov siab tshaj plaws nyob deb (± SD) ntawm maxillary canine txav ntawm lub lim tiam 3 yog 1.18 (± 0.04) mm ntawm sab laser thiab 0.85 (± 0.04) mm ntawm kev tswj sab, qhov sib txawv ntawm lawv yog qhov tseem ceeb (p <0.001). Txawm li cas los xij, qhov nruab nrab ntawm cov hniav txav tau txo qis ntawm lub lim tiam 6 ntawm ob lub laser thiab tswj sab, tom qab ntawd maj mam nce ntxiv tom qab 9 thiab 12 lub lis piam, nrog rau cov hniav txav tau ntau dua ntawm lub laser sab hauv kev sib piv nrog rau sab tswj (p <0.001), txhua lub sijhawm cov ntsiab lus. Txawm li cas los xij, qhov nruab nrab ntawm cov hniav txav tau txo qis ntawm lub lim tiam 6 ntawm ob lub laser thiab tswj sab, tom qab ntawd maj mam nce ntxiv tom qab 9 thiab 12 lub lis piam, nrog rau cov hniav txav tau ntau dua ntawm lub laser sab hauv kev sib piv nrog rau sab tswj (p <0.001), txhua lub sijhawm cov ntsiab lus.Txawm li cas los xij, qhov nruab nrab ntawm cov hniav tshem tawm tau txo qis ntawm lub lim tiam 6 ntawm ob lub laser thiab tswj ob sab, thiab tom qab ntawd maj mam nce thaum lub lis piam 9 thiab 12, nrog rau cov hniav tshem tawm ntau dua ntawm lub laser sab.laser piv nrog rau pawg tswj.стороны (p < 0,001) во все моменты времени. sab (p <0.001) txhua lub sijhawm.然而，激光侧和对照侧的平均牙齿移动量在第6周下降，然后在第9周和第12 帧幐后比，激光侧的牙齿移动量明显更高侧（p <0.001），在所有时间点。然而，激光侧和 对照侧的 牙齿移动量在第 6周下降，然后 第第第 周渐增加，与对照相比，的移动量明显 更高侧（p <0.001），在所有时间点。 Однако среднее количество движений зубов на стороне лазера и контрольной стороне умень шилось на 6-й, увеличилось через 9 и 12 недель, а количество движений зубов на стороне лазера было значительно выськние стороной (p <0,001) во все моменты времени. Txawm li cas los xij, qhov nruab nrab ntawm cov hniav txav ntawm sab laser thiab sab tswj tau txo qis ntawm lub lim tiam 6 thiab tom qab ntawd maj mam nce tom qab 9 thiab 12 lub lis piam, thiab cov hniav txav ntawm lub laser sab yog ntau dua piv rau cov tswj sab (p<0.001) ntawm txhua lub sijhawm. Tag nrho cov nyiaj ntawm cov hniav txav (± SD) nyob rau lub sijhawm kawm 12-lub lim tiam tau nce siab dua ntawm lub laser sab nrog 4.45 (± 0.13) mm, piv rau ntawm sab tswj uas yog 3.16 (± 0.14) mm (p <0.001). Tag nrho cov nyiaj ntawm cov hniav txav (± SD) nyob rau lub sijhawm kawm 12-lub lim tiam tau nce siab dua ntawm lub laser sab nrog 4.45 (± 0.13) mm, piv rau ntawm sab tswj uas yog 3.16 (± 0.14) mm (p <0.001). Общая величина смещения зубов (± SD) за 12-недельный период исследования была значительно выше на стормоне , 5 ± 0 ла сравнению с контрольной стороной, которая составляла 3,16 (± 0,14) м м (p < 0,001). Tag nrho cov nyiaj ntawm cov hniav tshem tawm (± SD) nyob rau lub sijhawm kawm 12 lub lis piam tau nce siab dua ntawm lub laser sab, 4.45 (± 0.13) mm, piv rau sab tswj, uas yog 3.16 (± 0.14) mm (p < 0.001).在为期12 周的研究期间，激光侧的牙齿移动总量(± SD) 显着更高，为4.45 (± 0.13) mm, 踌建 ± 6 䅅.001).在为期12 周的研究期间，激光侧的牙齿的牙齿纻动总量(± SD) 显着更高，为4.45 (± 0.13姯煯 16) mm ) (± 0.16) (± 0.16) (± 0.16 (± 0.16) (± 0.16) (± 0.16) В течение 12-недельного периода исследования общее перемещение зубов (± SD) было значительно выше на лстороно выше на лсороно 45 (± 0,13) мм по сравнению с 3,16 (± 0,14) мм в контрольной группе (p < 0,001). Thaum lub sijhawm kawm 12 lub lis piam, tag nrho cov hniav txav (± SD) tau nce siab dua ntawm lub laser sab ntawm 4.45 (± 0.13) mm piv rau 3.16 (± 0.14) mm hauv pawg tswj (p <0.001).
Hauv Pawg B, cov qauv zoo sib xws rau qhov pom hauv Pawg A tau ua raws, nrog qhov muaj txiaj ntsig ntau dua ntawm cov hniav txav tau raug kaw nyob rau sab laser, piv rau sab tswj txhua lub sijhawm (p <0.001). Hauv Pawg B, cov qauv zoo sib xws rau qhov pom hauv Pawg A tau ua raws, nrog qhov muaj txiaj ntsig zoo dua ntawm cov hniav txav tau raug kaw rau ntawm lub laser sab, piv rau sab tswj txhua lub sijhawm cov ntsiab lus (p <0.001). В группе B наблюдалась аналогичная картина, продемонстрированная в группе A, со значительно более виния волеке в группе бов, зарегистрированными на стороне лазера, по сравнению с контрольной стороной во все моменты вре 00 (0,000) Pawg B tau pom cov qauv zoo sib xws rau Pab Pawg A, nrog cov hniav txav ntau dua qhov tseem ceeb tau sau tseg ntawm lub laser sab piv rau sab tswj txhua lub sijhawm cov ntsiab lus (p <0.001).在B 组中，遵循与A 组相似的模式，与所有时间点的对照侧相比，激光侧记录的牙齿瀻。 ). <0.00 Nws В группе B, по аналогии с группой A, зарегистрированные значения перемещения зубов были зночительно на раночительно на раночительно внению с контрольной стороной во все моменты времени (p <0,001). Hauv pab pawg B, zoo ib yam li pawg A, cov ntaub ntawv khaws tseg ntawm cov hniav txav tau ntau dua ntawm lub laser sab piv rau kev tswj xyuas txhua lub sijhawm cov ntsiab lus (p <0.001).Tom qab 3 lub lis piam, qhov siab tshaj plaws ntawm cov hniav txav (± SD) tau kaw nrog tus nqi ntawm 1.14 (± 0.04) hli ntawm lub laser sab thiab 0.87 (± 0.03) hli ntawm kev tswj sab.Kev mob hniav tom qab txo qis ntawm lub lim tiam 6 thiab tom qab ntawd maj mam nce. Tag nrho cov nqi ntawm canine retraction (± SD) nyob rau 12-lub lim tiam kawm ntawm lub laser thiab tswj sab, yog 4.35 (± 0.12) mm, thiab 3.10 (± 0.06) mm, raws li, thiab qhov sib txawv ntawm lawv yog statisticically tseem ceeb (p <0.001). Tag nrho cov nqi ntawm canine retraction (± SD) nyob rau 12-lub lim tiam kawm ntawm lub laser thiab tswj sab, yog 4.35 (± 0.12) mm, thiab 3.10 (± 0.06) mm, raws li, thiab qhov sib txawv ntawm lawv yog statisticically tseem ceeb (p <0.001).Tag nrho cov canine retraction (± SD) nyob rau 12-lub lim tiam kev kawm ntawm lub laser thiab tswj sab yog 4.35 (± 0.12) mm thiab 3.10 (± 0.06) mm, ntsig txog, thiab qhov sib txawv ntawm lawv yog statistical tseem ceeb.(р <0,001). (p <0.001).在为期12 周的研究期间，激光侧和对照侧的犬齿回缩总量(± SD) 分别为 4.35 (± 0.13) mm (6.12mm), 10 mm (± 0.12) hli间的差异具有统计学意义(p <0.001).在为期 12 周的研究，激光侧和 对照侧的回缩总量总量总量 (± sd) 分别 5 ± 1 . 3 位(± 0.06) hli, 之间的差异具有统计学意义 (p <0.001). В течение 12-недельного периода исследования общая (± SD) ретракция клыка на стороне лазера и контрольной 0,12) мм и 3,10 (± 0,06) мм соответственно, и разница была статистически значимой (p < 0,001). Thaum lub sijhawm kawm 12 lub lis piam, tag nrho (± SD) canine retraction ntawm laser sab thiab tswj sab yog 4.35 (± 0.12) mm thiab 3.10 (± 0.06) mm, feem, thiab qhov sib txawv yog qhov tseem ceeb (p <0.001). .Table 4 piav qhia txog kev sib piv ntawm qib ntawm canine retraction ntawm lub sijhawm sib txawv ntawm laser thiab tswj sab hauv txhua pab pawg kawm.
Txawm hais tias qhov degree ntawm canine retraction los ntawm lub laser yog siab dua nyob rau hauv pab pawg A ntau dua nyob rau hauv pawg B nyob rau hauv tag nrho cov sij hawm cov ntsiab lus, qhov sib txawv no tsis suav hais tias tseem ceeb statistical piv rau pawg B (p = 0.08-0.55).Hais txog qhov feem pua ​​​​nce (± SD) hauv canine retraction ua tiav nrog txhua txoj cai, cov txheej txheem siv hauv pawg A tau nce los ntawm 40.78 (± 4.81)%, thaum cov txheej txheem siv hauv pawg A nce 40 .22 (± 4.80)% hauv pawg B. laser daim ntawv thov raws tu qauv tau txais.Txawm li cas los xij, txawm hais tias qhov feem pua ​​​​ntawm pawg A tau siab dua me ntsis hauv pawg B, qhov sib txawv ntawm lawv tsis yog qhov tseem ceeb (p = 0.82).Tsis tas li ntawd, nws tau pom tias qhov kev txav ntawm cov hniav hauv ob pawg kuj zoo ib yam (Fig. 7).
Laser retraction ntawm lateral canine (mm) ntawm lub sij hawm sib txawv cov ntsiab lus nyob rau hauv ob pab pawg neeg kawm nyob rau hauv lub 12-lub lim tiam txoj kev kawm.
Table 5 piav qhia txog qib ntawm IL-1β hauv pawg A thiab B ntawm txhua lub sijhawm ntsuas cov ntsiab lus ntawm lub laser thiab tswj sab.Hauv pab pawg A, qhov sib txawv ntawm laser sab thiab tswj sab ntawm lub hauv paus tsis tseem ceeb rau IL-1β qhov tseem ceeb (p = 0.56). Qhov siab tshaj plaws ntawm IL-1β (± SD) tau kaw nyob rau hnub 7 ntawm ob lub laser thiab tswj sab, nrog rau qhov tseem ceeb ntawm 0.152 (± 0.004) pg / ml / 60 s, thiab 0.127 (± 0.004) pg / ml / 60 s, raws li, thiab qhov sib txawv ntawm lawv yog <10. Qhov siab tshaj plaws ntawm IL-1β (± SD) tau kaw nyob rau hnub 7 ntawm ob lub laser thiab tswj sab, nrog rau qhov muaj nuj nqis ntawm 0.152 (± 0.004) pg / ml / 60 s, thiab 0.127 (± 0.004) pg / ml / 60 s, raws li, thiab qhov sib txawv ntawm lawv qhov tseem ceeb yog 0.0p (stat).Qhov siab tshaj plaws ntawm IL-1β (± SD) tau sau tseg rau hnub 7 ntawm ob lub laser thiab tswj sab nrog cov nqi ntawm 0.152 (± 0.004) pg/mL/60 s thiab 0.127 (± 0.004) pg/mL./60 с соответственно, а разница между ними была статистически значимой (p <0,001). /60s, feem, thiab qhov sib txawv ntawm lawv yog qhov tseem ceeb (p <0.001).在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD), 值为0.152 (± 0.004) pg/ml/60 ± 60 s. 1 g. , 它们之间的差异具有统计学意义 (p <0.001).在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD), 值为0.152 (± 0.004) pg/ml/60 ± 10 s.2 g. 0.001).Hnub 7, qhov siab tshaj plaws ntawm IL-1β (± SD) tau sau tseg ntawm ob lub laser thiab tswj sab nrog qhov tseem ceeb ntawm 0.152 (± 0.004) pg / mL / 60 s thiab 0.127 (± 0.004) pg / mL./60 s.Разница между ними была статистически значимой (p < 0,001). Qhov sib txawv ntawm lawv yog qhov tseem ceeb (p <0.001). Ib qho kev txo qis hauv IL-1β qib tau tshaj tawm tom qab ntawd, hnub 14 thiab 21, ntawm ob lub laser thiab tswj sab, nrog rau qhov tseem ceeb ntawm lub laser sab tau nce siab dua li ntawm cov tswj sab (p <0.001). Ib qho kev txo qis hauv IL-1β qib tau tshaj tawm tom qab ntawd, nyob rau hnub 14 thiab 21, ntawm ob lub laser thiab tswj sab, nrog rau qhov tseem ceeb ntawm lub laser sab tau nce siab dua li ntawm cov tswj sab (p <0.001). После этого сообщалось о постепенном снижении уровней IL-1β ntawm 14 thiab 21 день как на стороне лаозера, такя и на ом значения на стороне лазера были значительно выше, чем на стороне контроля (p <0,001). Tom qab ntawd, maj mam txo qis hauv IL-1β qib tau tshaj tawm nyob rau hnub 14 thiab 21 ntawm ob lub laser thiab tswj sab, nrog rau qhov tseem ceeb ntawm lub laser sab yog qhov siab dua li cov nyob rau sab tswj (p<0.001). .此后，在第14天和第21天，激光和对照侧的IL-1β 水平逐渐下降，的激光侧的值显着姫。 .此后，在第14天和第21天，激光和对照侧的IL-1β 水平逐渐下降，的激光侧的值显睾高 После этого на 14-й и 21-й дни уровни ИЛ-1β постепенно снижались на стороне лазера и в контроле, прия зататроле а были значительно выше, чем на стороне контроля (p <0,001). Tom qab ntawd, nyob rau hnub 14 thiab 21st, cov theem ntawm IL-1β maj mam txo qis ntawm lub laser sab thiab hauv kev tswj, thaum qhov tseem ceeb ntawm lub laser sab tau nce siab dua ntawm sab tswj (p <0.001).
Hauv pawg B, cov qauv zoo sib xws tau pom nyob rau hauv pawg A nrog rau qib IL-1β, nrog qhov sib txawv me me pom ntawm lub hauv paus ntawm laser thiab tswj sab (p = 0.02). Tom qab 7 hnub, lub ncov ntawm IL-1β theem (± SD) tau mus txog ntawm ob sab, nrog 0.139 (± 0.004) pg / ml / 60 s ntawm lub laser sab, thiab 0.122 (± 0.003) pg / ml / 60 s ntawm kev tswj sab, nrog rau cov nqi siab dua ntawm laser sab suav tias yog <0. Tom qab 7 hnub, lub ncov ntawm IL-1β theem (± SD) tau mus txog ntawm ob sab, nrog 0.139 (± 0.004) pg/ml/60 s ntawm lub laser sab, thiab 0.122 (± 0.003) pg/ml/60 s ntawm kev tswj sab, nrog rau cov nqi < ntawm lub laser sab 0. p.Tom qab 7 hnub, lub ncov theem ntawm IL-1β (± tus qauv sib txawv) tau mus txog ntawm ob sab: 0.139 (± 0.004) pg / ml / 60 s ntawm lub laser sab thiab 0.122 (± 0.003) pg / ml / 60 s.ntawm контрольной стороне, при этом значения на стороне лазера считались статистически более высокими,00 (p <1). nyob rau sab tswj, thaum qhov tseem ceeb ntawm lub laser sab raug txiav txim siab ntau dua (p <0.001). 7 天后, 两侧达到IL-1β水平峰值 (± SD), 激光侧为0.139 (± 0.004) pg/ml/60 s, 激光侧为 0.1203 / ± 0.激光侧的值在统计上更高 (p <0.001). 7 天后 ， 两侧 达到 达到 il-1β 水平 （±），激光 2 侧为 为 0.139 (± 0.004） 0 pg/ml/6 03) pg/ml/60 s 在 侧激光 激光 激光 激光 激光 激光 ， 在 ， 光 ， 光 ， 光 ，统计上更高 (p <0.001).Tom qab 7 hnub, qhov siab tshaj plaws ntawm IL-1β (± SD) tau ua tiav ntawm ob sab: 0.139 (± 0.004) pg / ml / 60 s ntawm sab laser thiab 0.122 (± 0.003) pg / ml / 60 s ntawm sab tswj., лазер Значения на стороне были статистически выше (p <0,001). , laser Values ​​​​ntawm ib sab tau txheeb xyuas ntau dua (p <0.001).IL-1β theem tom qab tau txo qis ntawm ob sab ntawm hnub 14 thiab 21, thiab cov qib sau tseg ntawm lub laser sab tau nce siab dua piv rau cov tswj sab ntawm ob lub sijhawm cov ntsiab lus (p = 0.001-0.002).Kev sib piv ntawm IL-1β qib ntawm lub sijhawm sib txawv ntawm lub laser sab thiab kev tswj xyuas hauv txhua pab pawg tau piav qhia hauv Table 6.
Thaum sib piv IL-1β theem ntawm ob pawg kev tshawb fawb, qhov tsis sib txawv tsis tseem ceeb tau sau tseg ntawm lub laser sab ntawm lub hauv paus (p = 0.96). Hnub tim 7 thiab 14, qhov sib txawv tseem ceeb tau sau npe ntawm cov laser sab hauv ob pawg, nrog cov txiaj ntsig siab dua ntawm cov laser sab hauv pawg A (p <0.001). Hnub tim 7 thiab 14, qhov sib txawv tseem ceeb tau sau npe ntawm cov laser sab hauv ob pawg, nrog cov txiaj ntsig siab dua ntawm cov laser sab hauv pawg A (p <0.001). На 7-й и 14-й дни зарегистрированы статистически значимые различия между лазерными сторонами в опрохонами в опрохонами высокие значения принадлежат лазерным сторонам в группе А (р < 0,001). Hnub 7 thiab 14, muaj qhov sib txawv tseem ceeb ntawm cov laser sab hauv ob pawg, nrog cov txiaj ntsig siab dua ntawm cov laser sab hauv pawg A (p <0.001).在第7 天和第14 天，两组激光侧的差异有统计学意义，A 组激光侧的值较高 (p <0.001). A На 7 thiab 14 дни разница между двумя группами была статистически значимой на стороне лазера с боляки выстороне лезера с боляки высме лазера в группе А (p <0,001). Nyob rau hnub 7 thiab 14, qhov sib txawv ntawm ob pab pawg tau txheeb xyuas qhov tseem ceeb ntawm lub laser sab, nrog cov txiaj ntsig siab dua ntawm lub laser sab hauv pawg A (p <0.001).Tom qab 21 hnub, tsis muaj qhov sib txawv ntawm ob pawg (p = 0.26).Cov theem ntawm IL-1β nyob rau hauv ob pawg muaj tib lub cim, ncav cuag qhov siab tshaj plaws nyob rau hnub 7 thiab maj mam txo qis rau hnub tim 14 thiab 21 (Fig. 8).
Lub hom phiaj ntawm txoj kev tshawb no yog qhov tseem ceeb los ntsuas thiab sib piv cov txiaj ntsig ntawm LILR ntawm canine retraction siv cov txheej txheem suav nrog kev siv hluav taws xob ntau zaus nyob rau hnub 0, 3, 7, 14 thiab txhua 2 lub lis piam tom qab ntawd (Pawg A) nrog cov neeg mob tsis ntev los no.muaj kev rov qab tsawg dua piv rau cov txheej txheem uas tau ua rau laser raug ntawm 3 lub lis piam (piv txwv li B).Txawm hais tias nws yog cov txheej txheem dav dav dav dav 7,13,26 lossis 3 lub lis piam raws tu qauv 15,17,18, ob txoj cai tau piav qhia hauv cov ntaub ntawv.Raws li cov txiaj ntsig tau nthuav tawm hauv txoj kev tshawb fawb tam sim no, qhov kev xav tsis zoo tsis raug tso tseg, thiab los ntawm kev siv ob txoj cai kawm, tus lej sib npaug ntawm tus dev txav tau tiav.
Txoj kev tshawb nrhiav tam sim no yog kev soj ntsuam randomized tswj kev sim (RCT).RCTs raug suav hais tias yog tus qauv kub rau kev ntsuas qhov cuam tshuam ntawm kev cuam tshuam27.Ib txoj kev sib cais lub qhov ncauj kuj tau siv, qhov txiaj ntsig tseem ceeb ntawm qhov uas yog qhov sib txawv ntawm cov ntsiab lus raug tshem tawm, nrog rau txhua tus neeg mob ua lawv tus tswj hwm, yog li txo cov neeg tuaj koom xav tau.
Txhua yam kev kawm suav nrog hauv txoj kev kawm yuav tsum tau muab rho tawm ntawm maxillary thawj premolar tom qab los ntawm canine retraction raws li ib feem ntawm kev kho mob orthodontic.Txij li thaum rho tawm tuaj yeem hloov pauv tus nqi ntawm RTM los ntawm kev nce cov kev ua ntawm cov cim inflammatory, uas nyob rau hauv lem tuaj yeem npog cov nyhuv ntawm LILT thiab muab cov ntawv nyeem tsis raug ntawm IL-1β qib thaum siv laser, kev tshem tawm kev kho mob tau ua ua ntej kev kho mob, uas muab cov txiaj ntsig zoo.Kho lub qhov (socket) extraction tov muab sij hawm txaus thiab kov yeej cov teebmeem ntawm lub regional acceleration phenomena28.Qhov kev ceev faj no kuj tau raug coj los ntawm qee tus kws sau ntawv, 11 uas tau tshawb xyuas cov txiaj ntsig ntawm LILT ntawm OTM tus nqi thaum lub sijhawm thim rov qab hauv cov dev los ntawm kev ntsuas qib ntawm biomarkers xws li IL-1β thiab hloov pauv qhov kev loj hlob β1 (TGF-β1) hauv GCF.
Hom laser siv nyob rau hauv txoj kev tshawb no yog ib tug diode semiconductor laser siv ntawm 980 nm raws li cov chaw tsim tshuaj paus cov lus pom zoo rau kev pom biostimulation.Qhov no tuaj yeem piav qhia los ntawm qhov tseeb tias qhov ntev ntawm lub laser wavelength (650-1200 nm), qhov tob ntawm cov ntaub so ntswg nkag mus rau 29.Txawm li cas los xij, qhov kev pom zoo wavelength no tau siv nyob rau hauv ntau lwm cov kev tshawb fawb, ua rau muaj txiaj ntsig zoo nrawm ntawm 8.30 thiab qhov tsis zoo ntawm 14.
Lwm qhov tseem ceeb cuam tshuam rau kev ua tau zoo ntawm LILI kev kho mob thiab biostimulation yog koob tshuaj lossis lub zog ntom ntom.Thaum tshuaj xyuas cov ntaub ntawv, nws tau pom tias muaj qhov sib txawv loj hauv qhov ntau npaum li cas ntawm LILI lub zog kom nrawm rau GTM.Qee tus kws sau ntawv tau tshaj tawm cov txiaj ntsig zoo thaum siv lub zog qis los ntawm 0.7131, 532.33, 7.514 txog 8 J / cm234.35, thaum lwm tus kws tshawb fawb kuj tau tshaj tawm cov txiaj ntsig ntawm LILR ntawm GTM tus nqi ntawm lub zog siab dua, piv txwv li , 25 J / cm2.xwm 27.36.Hauv kev ua haujlwm tam sim no, ib koob ntawm lub zog qis laser ntawm 8 J / cm2 tau xa los ntawm ib qho kev cuam tshuam rau maxillary canine hauv paus rau 8 vib nas this siv lub tiaj tiaj sab saum toj los faib cov kab teeb ntawm 1 cm2.Muaj kev sib raug zoo ntawm cov nqaj loj thiab qhov tob tob ntawm lub laser, uas ua rau pom tseeb ntawm kev siv cov khoom siv sab saum toj hauv qhov kev kawm no29,37.Tib daim ntawv thov raws tu qauv nrog cov kab teeb loj loj yog ua nrog kev sib tw thiab kev sib tw 8 thiab canine retraction 38 .
IL-1β paub tias yog ib qho tseem ceeb ntawm cov cytokine thaum pib ntawm OTM thiab suav tias yog ib qho cim ntawm pob txha resorption.Yog li ntawd, IL-1β theem tau raug soj ntsuam los ntawm laser hauv ntau qhov kev tshawb fawb 11,39,40 hauv kev sim txiav txim siab lawv cov kev sib raug zoo.Hauv kev sim tam sim no, IL-1β theem hauv GCF tau soj ntsuam ntawm qhov kev sim thiab kev tswj xyuas ntawm txhua pab pawg los ntawm kev siv ob txoj kev sib txawv ntawm LILI hnub 0, 7, 14, thiab 21.
Nyob rau hauv txoj kev tshawb no, canine retraction los ntawm lub laser nyob rau hauv pawg A thiab B yog ho siab tshaj nyob rau hauv lub tswj pab pawg neeg ntawm txhua lub sij hawm cov ntsiab lus ntsuas, peaking ntawm lub lim tiam 3, poob rau ib lub lim tiam ntawm lub lim tiam 6, thiab ces maj mam nce mus rau lub lim tiam 12..Peak canine txav tau sau tseg nyob rau lub lim tiam 3 tuaj yeem piav qhia los ntawm cov txiaj ntsig ntawm thawj cov hniav tshem tawm, suav nrog: cov hauv paus hloov pauv hauv PDL, pob txha deformity vim flexure thiab creep, thiab compressive siab ntawm cov hniav vim qhov inclination ntawm lub conical socket Plane effect 41. Tsis tas li ntawd, nws tau pom tias tag nrho cov txheej txheem lom neeg ntawm cov pob txha tau nrawm dua.Qhov kev poob qis tom qab pom ntawm 3 thiab 6 lub lis piam, tej zaum vim yog lub sijhawm ncua uas tuaj yeem sib txawv ntawm 2 mus rau 10 lub lis piam, yog lub sijhawm ntawm PDL cuam tshuam uas resorbs thiab tshem tawm cov pob txha uas nyob ib sab ntawm thaj tsam crush, tso cai rau pob txha txav.cov hniav.Lwm qhov ua tau zoo rau qhov kev soj ntsuam no yog tias cov pa oxygenated fibers, collagen fibers, thiab kho pob txha alveolar nyob rau sab ntawm qhov nro kuj tseem tuaj yeem txo tus nqi ntawm cov hniav.Cov qauv zoo sib xws ntawm cov hniav txav tau pom nyob rau hauv ib qho kev kawm cleft45 piv cov teebmeem ntawm LILI thiab corticotomy ntawm canine retraction tus nqi, lawv tau sau tseg tias cov hniav txav tau zoo tshaj plaws ntawm lub lis piam 2 thiab 5, tom qab ntawd poob qis ntawm 2 thiab 5 lub lis piam.lub week.Qhov no tsis tau tshaj tawm ntawm sab laser ntawm lub lim tiam 7, tab sis tsis yog nyob rau sab corticotomy.
Cov lus tshaj tawm txhais tau tias feem pua ​​​​nce ntxiv ntawm qhov kev ncua deb ntawm kev txav ntawm maxillary canine los ntawm sab ntawm lub laser yog 40.78% hauv pab pawg A thiab 40.22% hauv pawg B. Qhov pom tseeb nce hauv cov hniav txav uas nrog kev siv lub laser tuaj yeem piav qhia ntawm qib cellular los ntawm kev nqus ntawm lub zog laser los ntawm photoreceptors nyob rau hauv cov saw hlau hauv electron-hloov daim nyias nyias.Cov nyhuv no ua rau lub sijhawm luv luv ntawm cov kab mob ua pa, uas ua rau oxidative phosphorylation thiab hloov pauv hauv lub xeev redox ntawm cellular mitochondria thiab cytoplasm.Nyob rau hauv lem, lub zog tsav ntawm lub cell yog nce los ntawm kev nce cov khoom ntawm ATP.Tsis tas li ntawd, muaj kev nce hauv lub peev xwm ntawm mitochondrial membrane, alkalization ntawm cytoplasm, thiab cov synthesis ntawm nucleic acids.Txij li thaum ATP paub tias yog lub zog txiaj ntawm cov hlwb, LILI pab txhawb kev ua haujlwm ntawm cov hlwb los ntawm kev tsim ib qho chaw zoo rau cov hniav txav46.Yog li, los ntawm peb cov txiaj ntsig, peb tuaj yeem txiav txim siab tias kev siv LILT ua ib qho ntxiv rau kev kho hniav tuaj yeem ua tiav OTM tsis hais seb nws siv ntau npaum li cas hauv pawg A (hnub 0, 3, 7, 14 thiab txhua hnub).tom qab 2 hnub) lub lis piam), lossis yog siv tsawg zaus hauv pawg B (txhua 3 lub lis piam), yog li ntawd, qhov kev xav tsis zoo tsis raug tso tseg.
Qhov cuam tshuam zoo ib yam ntawm ob qhov kev sim LILT cov txheej txheem qhia hauv qhov kev tshawb fawb no tuaj yeem yog vim muaj qhov pib ua kom lub cellular uas nce cellular activation nrog LILT raug tshwm sim thawj zaug, tab sis tom qab ntawd rov ua dua (raws li hauv pab pawg A), vim yog cov tshuaj lom neeg saturated yuav tsis ua rau muaj kev ua kom ntxiv.Yog li, peb tuaj yeem xav tias qhov cuam tshuam ntawm LLLT ntawm qib cellular tsis tuaj yeem suav nrog.Hais txog kev sib raug zoo ntawm qib quab yuam thiab cov hniav txav ceev, lub tswv yim ntawm biosaturation tau piav qhia yav dhau los.
Tom qab tshuaj xyuas cov ntaub ntawv uas twb muaj lawm, peb muab piv rau 1.4-fold (40-41%) nce hauv WTM tau txais hauv peb txoj kev tshawb fawb siv ob lub laser raws tu qauv nrog cov txiaj ntsig ntawm ntau lwm cov ntawv ceeb toom.Qee qhov kev tshawb fawb tau tshaj tawm cov txiaj ntsig zoo sib xws 11,30,48,49 thaum lwm tus tau tshaj tawm cov nqi qis dua me ntsis siv LILI7,18,32,40.Ntawm qhov tod tes, ntau qhov kev nrawm nrawm dua li cov uas tau tshaj tawm hauv kev sim tam sim no, xws li 1.65 × 17 mus rau ze li 2x OTM15, 34, 39, 50, uas tej zaum yuav cuam tshuam rau qee qhov ntawm lawv siv tus kheej-locking braces yam tsis muaj kev sib txhuam 15. Qhov sib txawv ntawm cov txiaj ntsig tau luam tawm nyob rau hauv cov ntaub ntawv siv hluav taws xob siv hluav taws xob, lub sij hawm sib txawv ntawm kev siv hluav taws xob, kev siv hluav taws xob sib txawv, yoj pos huab. ity, kev kho lub sijhawm, thiab lwm yam, uas ua rau kev sib piv ncaj qha ntawm cov kev tshawb fawb sib txawv nyuaj heev..Txawm li cas los xij, nws tau raug sau tseg tias lub zog qis dua (piv txwv li 2.5, 5 thiab 8 J / cm2) muab kev ua haujlwm nrawm dua piv rau cov zog siab dua, nws tsim nyog sau cia tias cov koob tshuaj siv hauv peb qhov kev sim yog 8 J / cm2.cm2 ua.
Kev txhais lus ntawm IL-1β theem nyob rau hauv qhov distal cleft (compression side) tom qab kev soj ntsuam ntawm cov GCF cov qauv tau pom ib qho kev nce ntxiv ntawm cov hauv paus ntsiab lus (piv txwv li ncov) hnub 7 tom qab los ntawm kev maj mam txo mus rau hauv paus.ntawm panels A thiab B, nyob rau sab laser thiab sab tswj.Qhov no tuaj yeem piav qhia los ntawm qhov tseeb tias thawj theem ntawm OTM feem ntau yog nrog los ntawm kev nce hauv kev ua haujlwm osteoclast.IL-1β kuj tseem suav tias yog tus cim tshawb pom ntxov tshaj plaws uas cuam tshuam nrog cov pob txha resorption, thiab IL-1β qhia tau hais tias yuav nce ntxiv nrog quab yuam thiab tom qab txo qis hauv ntau qhov kev tshawb fawb11,20,51.
Tsis tas li ntawd, IL-1β qib siab dua nyob rau sab laser piv rau pawg tswj hwm hauv ob pawg kev tshawb fawb ntawm txhua lub sijhawm ntsuas cov ntsiab lus tshwj tsis yog lub hauv paus, thiab muaj qhov sib txawv tseem ceeb ntawm lawv.Qhov no qhia tau hais tias tsis tshua muaj siv laser irradiation ua rau muaj kev txhim kho lom neeg teb nyob rau hauv cov ntaub so ntswg periodontal ntawm qhov kev sim sab nyob rau hauv daim ntawv ntawm stimulation ntawm osteoclast muaj nuj nqi ntawm compressed sab thaum orthodontic hniav txav.Cov nyhuv no ntawm LLLT ntawm IL-1β theem tau pom nyob rau hauv ntau yam kev tshawb fawb11,39,40.
Thaum muab piv rau qib laser-side IL-1β hauv ob pawg kev tshawb fawb, qib tau txheeb xyuas ntau dua hauv pab pawg A piv rau pawg B ntawm hnub 7 thiab 14. Qhov no tuaj yeem piav qhia los ntawm ntau qhov cuam tshuam rau laser irradiation hauv pab pawg A thaum lub sijhawm 21-hnub soj ntsuam, qhov twg irradiation tau nqa tawm hnub 0, 3, 4, 7, thiab 1 hnub nkaus xwb. IL-1β qib tau txheeb xyuas ntau dua ntawm lub laser sab hauv pawg A, qhov sib txawv ntawm cov ntaub ntawv no tsis tau pom zoo hauv kev kho mob hauv cov dev piv rau laser sab hauv pawg B, vim tias tsis muaj qhov tseem ceeb ntawm kev txheeb cais.Hauv pab pawg A thiab B, qhov qhia txog qhov sib txawv ntawm canine retraction ntawm laser sab tau ua rau tib tus nqi ntawm canine txav.Yog li ntawd, peb tuaj yeem hais tias qhov sib txawv ntawm kev txheeb cais tsis tas yuav piav qhia qhov tseem ceeb ntawm kev kho mob.
Kev siv laser siv tsawg, thaum siv nrog cov tsis siv hauv txoj kev tshawb no, tuaj yeem ua kom lub zog ntawm cov hniav orthodontic zoo li ntawm 1.4 zaug, txawm tias nws siv los ntawm qhov siab lossis qis zaus, coinciding nrog kev soj ntsuam tas li, tej zaum.tsim nyog rau cov neeg mob.
Ib qho kev nce hauv cov hniav txhuam hniav thaum lub sij hawm LILI tau nrog los ntawm kev nce qib ntawm interleukin-1β ntawm sab compressed, uas qhia tau hais tias kev siv LILI ua rau cov txheej txheem txhim kho pob txha.
Cov ntaub ntawv siv thiab / lossis tshuaj ntsuam hauv txoj kev tshawb fawb tam sim no muaj los ntawm cov kws sau ntawv raws li qhov kev thov tsim nyog.
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