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Injongo yolu phononongo yayikukuvavanya izinga lokurhoxiswa kwe-canine kwiirejimeni ezimbini ze-laser therapy (LLLT) ze-irradiation ezimbini, kubandakanywa i-frequencies ephezulu kunye nephantsi.Izigulane ezingamashumi amabini zahlulwa ngokungenamkhethe zibe ngamaqela amabini.Kwiqela le-A, elinye icala le-maxillary arch lalingenamkhethe ukufumana i-LILT ngeentsuku ze-0, 3, 7, 14, kunye neeveki zonke ze-2 emva koko, ngelixa iqela B, elinye icala lafumana i-LILT rhoqo kwiiveki ze-3.Ngethuba lokufunda kweeveki ze-12, ukuhamba kwamazinyo kwajongwa rhoqo kwiiveki ezintathu ukususela ekuqaleni kwe-canine retraction.Ukongezelela, amanqanaba e-interleukin-1β (IL-1β) kwi-gingival sulcus fluid ahlolwe. Iziphumo zibonise ukunyuka okuphawulekayo kwinqanaba lokurhoxiswa kwe-canine kumacala e-laser amaqela A kunye ne-B, xa kuthelekiswa namacala okulawula (p <0.05), kungekho nantlukwano ebalulekileyo echazwe phakathi kwamacala e-laser kumaqela omabini (p = 0.08-0.55). Iziphumo zibonise ukwanda okuphawulekayo kwinqanaba lokurhoxiswa kwe-canine kumacala e-laser amaqela A kunye ne-B, xa kuthelekiswa namacala okulawula (p <0.05), kungekho nantlukwano ebalulekileyo echazwe phakathi kwamacala e-laser kumaqela omabini (p = 0.08-0.55). Результаты выявили значительное увеличение скорости ретракции клыков на стороне лазера в группах A kunye B по сравнению с контрольной 5 стох0 различий между сторонами лазера в обеих группах (p = 0,08–0,55). Iziphumo zibonise ukwanda okukhulu kwesantya sokurhoxisa i-canine kwicala le-laser kumaqela A kunye ne-B xa kuthelekiswa necala lokulawula (p <0.05), kungekho mmahluko omkhulu phakathi kwamacala e-laser kumaqela omabini (p = 0.08-0.55). ).结果显示，与对照组相比，A 组和B 组激光侧的犬齿回缩率显着增加（p <0.05光侧的犬齿回缩率显着增加（p <0.05光显旉，着差异 (p = 0.08-0.55).结果 显示 , 与 对照组 , 组和 a 组和 b 组 激光侧 犬齿 回 缩率 显着 （（组 激光侧 犬齿 回 缩率 显着 （（组光间 显着 差异 （p = 0.08-0.55。。。. Результаты показали, что по сравнению с контрольной группой скорость ретракции клыков на стороне лазера в группах А и В быльков на стороне лазера в группах А и В быльков не лазера не было существенной разницы между двумя группами (p = 0,08-0,55). Iziphumo zibonise ukuba, xa kuthelekiswa neqela lokulawula, izinga lokurhoxiswa kwe-canine kwicala le-laser kumaqela A kunye ne-B laliphezulu kakhulu (p <0.05), kwaye kwakungekho nantlukwano enkulu phakathi kwamaqela amabini kwicala le-laser (p = 0.08-0 .55). Kwakhona, amanqanaba e-IL-1β ayephezulu kakhulu kumacala e-laser amaqela omabini, xa kuthelekiswa namacala okulawula (p <0.05). Kwakhona, amanqanaba e-IL-1β ayephezulu kakhulu kumacala e-laser amaqela omabini, xa kuthelekiswa namacala okulawula (p <0.05). Кроме того, уровни IL-1β были значительно выше на стороне лазера в обеих группах по сравнению с контрольной стороной (p <0,05). Ukongezelela, amanqanaba e-IL-1β ayephezulu kakhulu kwicala le-laser kumaqela omabini xa kuthelekiswa necala lokulawula (p <0.05).此外，与对照组相比，两组激光侧的IL-1β 水平显着升高（p <0.05）.此外，与对照组相比，两组激光侧的IL-1β 水平显着升高（p <0.05）. Кроме того, уровни IL-1β были значительно повышены на стороне лазера в обеих группах по сравнению с контрольной группой (p <0,00). Ukongezelela, amanqanaba e-IL-1β aphakanyiswe kakhulu kwicala le-laser kumaqela omabini xa kuthelekiswa neqela lokulawula (p <0.05).Ngaloo ndlela, i-LILI yakwazi ukukhawuleza ukukhawuleza ukuhamba kwamazinyo, nokuba isetyenziswe rhoqo okanye inqabile, eyayidibene nokunyuka kwempendulo yezinto eziphilayo, ebonakaliswe kumanqanaba okwanda kwe-IL-1β kwicala elixinyiweyo.
Unyango lwexesha elide lwe-orthodontic (ngokuqhelekileyo malunga ne-20-30 iinyanga1) lufunyenwe luchaphazela kakubi ukuthotyelwa kwesigulane, ngaphezu kweengozi ezifana neengcambu ze-resorption2, i-caries3, i-enamel decalcification3 kunye neengxaki ze-periodontal4,5.Ngoko ke, iindlela ezininzi ziye zacetywayo ezijoliswe ekukhawuleziseni i-orthodontic tooth movement (OTM), kubandakanywa ukunakekelwa kokuhlinzwa kunye nokungabikho kokuhlinzwa.Ukongezelela, umphumo wokudibanisa iindlela ezimbini zokukhawuleza kunye nomphumo wokuphindaphinda inkqubo efanayo yokukhawuleza kwisantya se-OTM6 yaphandwa.
I-Low intensity laser therapy (LLLT) ibe yenye yeendlela ezicetywayo ezingezizo utyando ukukhawulezisa i-OTM, kodwa kukho iziphumo eziphikisanayo kwiingxelo zokusebenza kwayo kule ndawo, ngelixa iziphumo ezi-positive7,8 kunye ne-negative9 zibhaliwe.Ezi ziphumo eziphikisanayo zingachazwa ngokungafani kwiiparamitha ze-laser ezisetyenziswa kwisifundo ngasinye, kubandakanywa uhlobo lwe-laser, indlela yokusetyenziswa, ubude be-wavelength, umthamo we-radiation, kunye nexesha lokuvezwa, ekubeni ezi parameters zihambelana ngqo neziphumo zeklinikhi ze-laser application 10.
Ngokweendlela zokusetyenziswa, iiprotocol ezahlukeneyo ze-laser irradiation ziye zabikwa ukuba ziququzelele ukuhamba kwamazinyo.Iprothokholi enye esetyenziswa ngokubanzi ibandakanya ukusetyenziswa kwelaser ngeentsuku ze-0, 3, 7, 14, 21 kunye ne-30, ukuphinda ulandelelwano olufanayo nyanga zonke, kwaye le protocol yamkelwe ngababhali abaliqela11,12.Abanye basebenzise enye irejimeni esondele kakhulu kwirejimeni echazwe ngaphambili kwaye ikwayenye yeendlela ezisetyenziswa ngokubanzi, apho i-LILI isetyenziswa ngeentsuku ze-0, 3, 7, 14, kwaye emva kweentsuku ezili-15 de kuphele ixesha lophononongo.13. Ukongezelela, kuye kwacetywa iprotocol ebandakanya ukusetyenziswa kweveki nganye kwelaser ephantsi kakhulu kwixesha lonke lokurhoxiswa kwe-canine.Nangona kunjalo, eyona nto ingalunganga kwezi prothokholi ziqhelekileyo lizinga eliphezulu lempendulo yesigulana, enokuthi iphazamise wonke umntu.Ke, iiprothokholi ezifuna ukuthunyelwa kwezigulane ezimbalwa zisetyenziswa, umzekelo, kubandakanya i-LILI 8 amaxesha ngenyanga okanye i-15, 16, 17, 18 rhoqo kwiiveki ezi-3.
Ekubeni amandla e-orthodontic ayaziwa ngokubangela ukuguqulwa kwamathambo, ukuphuhliswa kweenguqu ezivuthayo ziyimfuneko kule nkqubo, ekhokelela ekungalungisweni kakuhle kwamazinyo19.Ngokwezifundo ezininzi, enye indlela yokuvavanya iziganeko zebhayoloji ezinokuthi zibekho kwi-periodontal ligament kukuvavanya inqanaba le-cytokines kwi-gingival sulcus fluid (GCF).I-Interleukin-1β (IL-1β) i-cytokine esebenzayo kakhulu kwi-metabolism yamathambo kwaye ithathwa njengenye ye-cytokines enamandla kakhulu kwi-OTM yokuqala ye-periodontal tissue.Ekubeni kukho ukulungelelaniswa phakathi kwamanqanaba e-IL-1β kunye nokusinda, ukudibanisa kunye nokusebenza kwe-osteoclast, i-IL-1β inokuthathwa njengophawu olubalulekileyo lokubala i-degree of orthodontic tooth movement, ehambelana nokusebenza kakuhle kwe-alveolar bone remodeling24.
Ke ngoko, injongo yophononongo lwethu yayikukuvavanya kunye nokuthelekisa iziphumo ze-NILT kunye neerejimeni ezisetyenziswa ngokuqhelekileyo, kubandakanywa ukuphindaphinda okuphezulu kokusetyenziswa kwiintsuku ze-0, 3, 7, 14, kwaye emva kweeveki ze-2 xa kuthelekiswa nokusetyenziswa rhoqo kwiiveki ze-3.Izinga lokurhoxisa kwizinja kwinzame zokunciphisa ukuphindaphinda kwezigulane ezikhumbulayo.Ukongeza, amanqanaba e-IL-1β kwi-GCF ahlolwe kusetyenziswa iiprotocol ezimbini.I-null hypothesis yophononongo lwangoku kukuba akukho mmahluko kwisiganeko sokurhoxiswa kwe-canine kunye ne-LILI usebenzisa iiprothokholi ezimbini zokuvavanya.
Uphononongo lwalululingo lwekliniki olulawulwa ngokungenamkhethe kunye namaqela amabini ahambelanayo, ngalinye livavanya iprotocol ye-LILI.Iqela ngalinye lamkela uyilo lomlomo olwahlulahlulwe, icala elinye liqela lolawulo kwaye elinye liqela lokufunda.
Uphononongo lubandakanya izigulane ze-20 ezineminyaka eyi-15 ukuya kwi-20 iminyaka efuna ukususwa kwonyango lwe-premolars yokuqala yomhlathi ophezulu, elandelwa ukuhoxiswa kwee-canines.Ubalo lobungakanani besampulu lusekelwe kwimpazamo ye-alpha ye-5% kunye namandla okufunda angama-80%.Olu balo lusekwe kwintsingiselo kunye nokutenxa okusemgangathweni kwe-canine retraction kwizifundo apho u-Doshi-Mehta kunye ne-Bhad-Patil7 basebenzise i-LILI ngeentsuku ze-0, 3, 7, 14 kunye neeveki ezi-2 emva koko (iArm A) nakwizifundo ze-Qamruddin et al.abanye Kwizifundo ezili-15, i-LILI yayisetyenziswa rhoqo kwiiveki ezi-3 (iqela B).Imvume yokuziphatha yafunyanwa kwi-Ethics Council ye-Faculty of Dentistry, iYunivesithi yase-Alexandria, e-Alexandria, eYiputa (IRB: 00010556-IORG: 0008839).Inani lekomiti yemigaqo yokuziphatha ngu-0111-01/2020.Ivunywe nge-21 kaJanuwari 2020. Ulingo lubhaliswe ne-ClinicalTrials.gov "njengeeProtokholi zeLaser ezimbini eziPhantsi zokuHlola isantya sokurhoxa kwizinja."Inombolo yobhaliso lwesilingo ngu-NCT04926389.Umhla wovavanyo lobhaliso yi-06/15/2021 apha https://clinicaltrials.gov/ct2/show/NCT04926389.Ukubhaliswa kwezigulana kuphononongo kuqale ngoFebruwari 5, 2020 kwaye kwaphela ngoNovemba 28, 2021.
Izigulana zaqeshwa kwiklinikhi ye-orthodontic yeFaculty of Dentistry yeYunivesithi yase-Alexandria.Izifundo zihlolwe kwaye zavavanywa ngokubhekiselele kwiindlela zokufaneleka ezilandelayo: impilo jikelele, ukungabikho kwesifo esingapheliyo, akukho unyango lwangaphambili lwe-orthodontic, ukucoceka komlomo okwaneleyo, kunye nezicubu ze-periodontal ezinempilo.Izigulane ezithatha inxaxheba kunye nabazali bazo zanikwa inkcazo epheleleyo kunye ecacileyo yeenkqubo zokufunda, kwaye ngoko ke, imvume enolwazi yafunyanwa kwisifundo ngasinye esibandakanyiweyo.Zonke iinkqubo zophando zenziwa ngokuhambelana nezikhokelo ezifanelekileyo kunye nemithetho echazwe kwiSibhengezo saseHelsinki.
Ngaphambi kokuqala ukuhoxiswa kwe-canine, izigulane ze-20 zakhethwa kwaye zabelwa ngokungaqhelekanga kwiqela A okanye iqela B (i-10 kwiqela ngalinye) kwi-low-intensity laser therapy.I-Randomization yenziwa ngokusebenzisa inkqubo elula yokwenziwa kwe-randomization kunye nomlinganiselo wokusabalalisa we-1: 1.Kwalungiselelwa ibhokisi enamaphepha angamashumi amabini asongiweyo, alishumi kuwo abhalwe amagama athi “Iqela A” aze amanye alishumi abhalwe “Iqela B”.Umthathi-nxaxheba ngamnye wacelwa ukuba akhethe iphepha elisongiweyo ebhokisini aze abele elinye lamaqela amabini ngokufanelekileyo.Inkqubo efanayo iphinda iphindwe kwakhona kwiqela ngalinye, ukutyumba elinye icala le-maxillary arch njenge "uvavanyo" kunye nelinye icala "njengolawulo" kwi-design-mouth-design.
Ukongeza kwiirekhodi eziqhelekileyo ze-orthodontic (iifoto ze-intraoral kunye ne-extraoral, i-radiographs, kunye nokubonakala kwamazinyo), izifundo ezilungiselelwe unyango olusisigxina lwamathambo zabhaliswa ngokuqulunqa iimbali zabo zonyango kunye namazinyo.Kwakhona izigulane zacelwa ukuba zenze ucoceko olupheleleyo lomlomo kunye nokupholisha okulandelwa ngumyalelo wococeko olufanelekileyo lomlomo (ukusetyenziswa kwebrashi yamazinyo, ifloss kunye neebrashi zamazinyo).
Ukulungiswa kwe-maxillary kunye ne-mandibular kunye nezixhobo ze-Roth zocingo ezithe ngqo (i-Mini 2000; i-Ormco, i-USA) kunye ne-0.022"\(\x) 0.028" iindawo zokubeka zaqiniswa kuzo zonke izigulane eziqeshwe, apho inkqubo yokulungiswa yayisemgangathweni kuwo omabini amaqela kwaye inqunywe ngumqhubi ofanayo..Emva koko, isigulane sathunyelwa kwi-extraction ye-maxillary yokuqala ye-premolar ukuvumela ixesha elaneleyo lokuba i-socket iphilise emva kokukhutshwa ngaphambi kokuqala ukukhutshwa malunga neenyanga ze-2 emva kokukhutshwa.Ulungelelwaniso luqale kwaye ulungelelwaniso lugqitywe xa i-0.016″ x 0.022″ yocingo lwentsimbi engatyiwayo inokufakwa ngokuqhubekayo kuwo onke amazinyo amakhulu.
Ngaphambi kokuqala ukurhoxiswa kwe-canine, i-premolars yesibini ephezulu kunye ne-molars yokuqala yahlanganiswa kunye ne-0.009-intshi ye-figure-wire wire kumacala okulinga kunye nokulawula amaqela omabini.Ukongezelela, i-incisors ye-maxillary iboshwe kunye ngendlela efanayo necandelo elingasemva ukunceda ukuzinzisa nokukhusela ukuhlukana kwabo okunokwenzeka.
Ukuhoxiswa kweCanine kumaqela A kunye no-B kwenziwa ngokusebenzisa i-nickel-titanium (i-NiTi) evaliweyo imithombo yekhoyili (i-Ormco, e-USA), zombini kumacala okulinga kunye nokulawula, yolulelwe phakathi kwamagwegwe e-canine brackets kunye namagwegwe kwi-canal ye-molar, kunye ne-force ye-150 g ilinganiswa ne-dynamometer (i-Morelli, eBrazil).
I-laser diode (Wiser; Doctor Smile-Lambda Spa, Brendol, Italy) isetyenziswe njengelaser ephantsi, ikhupha i-radiation ye-infrared ene-wavelength ye-980 nm kunye negunya eliphumayo le-100 mW kwimodi eqhubekayo.I-fiber wave wave (AB 2799; UGqirha Smile-Lambda Spa, Brendola, Italy) yasetyenziselwa ukusasaza indawo ye-1 cm2 ye-beam kunye nencam ethe tyaba ephezulu, ibeka incam yefiber ecaleni kwe-arch ye-maxillary embindini wesithathu we-maxilla.ingcambu ye-canine kwicala lokulinga (ngokwemiyalelo yomenzi, ubuncinci be-1.5 cm xa ungekho kugxininiso) imizuzwana eyi-8 (umzobo 1).Ubuninzi bamandla obuninzi obusetyenzisiweyo ngesiqendu yi-8 J / cm2 (1 J / cm2 ngesibini).Iiparamitha ze-laser ezisetyenzisiweyo ziboniswa kwiThebhile 1. Izilumkiso zithathwe ngaphambi kokusebenzisa i-laser, kwaye zombini isigulane kunye nomsebenzisi wasebenzisa izibuko ezibonelelwe ngumenzi, kuxhomekeke kubude obusetyenzisiweyo.
I-fiber tip yayibanjwe kumgama we-1.5 cm ukusuka kwingcambu ye-maxillary canine kwicala lokulinga ngokwemiyalelo yomenzi.
Ubuchule bokwahlula-umlomo busetyenziswe kumaqela omabini, kwaye umthathi-nxaxheba ngamnye u-randomized ukufumana i-LILI kwelinye icala le-maxillary arch kwaye kwelinye icala njengolawulo.Kwiqela A, izifundo zafumana i-LILT ngeentsuku ze-0, i-3, i-7, i-14 kwaye emva kweeveki ze-2, ngelixa kwiqela le-B lisetyenziswe rhoqo kwiiveki ze-3 kwicala lokulinga lonke ixesha lokufunda (iiveki ze-12) ze-LILT.I-laser beam nayo yayigxininiswe ngokungahambiyo kwicala lolawulo lwamaqela omabini, ukubonelela ngempembelelo ye-placebo njengenxalenye yenkqubo yokumfamekisa izigulane ezibhalisiweyo.Ngenxa yobume bongenelelo kweli nqanaba, umqhubi akanakukhohliswa.
Ngaphambi kokuqokelela i-specimen, macala omabini ama-maxillary canines ahlanjululwa nge-cotton swabs, eyedwa kunye ne-self-supporting retractors, i-suction, kunye ne-cotton rolls, kwaye emva koko i-air-drive ngobumnene i-5 s.Iisampulu zithathwe kwii-distal clefts ze-maxillary canines zisebenzisa i-standard strips paper filters (Whatman, Maidstone, UK) kwaye zinqunywe kwiisayizi eziqhelekileyo ze-2 × 10 mm2.Faka ngobunono umgca ngamnye kwisikhewu ude uzive uxhathisa kancinane, uze uyishiye kwindawo imizuzwana engama-60 ngelixa ugcina ukutywinwa ngokufanelekileyo (Umfanekiso 2).Emva kokususwa, imichilo emitsha ibekwe yonke iminzu eyi-1 ukufumana imicu emi-4 kwindawo nganye.Amanyathelo nawo athatyathwa ukuphepha umonakalo womatshini kwi-fissure ye-gingival.Lahla iisampulu ezingcoliswe ngamathe okanye igazi kwaye uqokelele iisampuli ezintsha.Iisampulu ze-GCF zithathwe kwisiseko (ngaphambi kokuqala kwe-canine retraction), ukusuka kwi-distal canine fissures kumaqela A kunye no-B, kumacala okulinga kunye nokulawula, ngaphandle kweentsuku ze-7, i-14 kunye ne-21.
Imibono ye-Alginate (i-Ca37; i-Cavex, i-Haarlem, i-Netherlands) yenziwa ngaphambi kokurhoxiswa kwe-canine kwaye iphinda iphindwe rhoqo kwiiveki ze-3 ngexesha le-12-iveki yokufunda kwityelelo ngalinye.Kutyelelo ngalunye, iingcingo zocingo kunye nemithombo yekhoyili zazikhutshwa, kuthatyathwe umfanekiso we-alginate, kwaza kwaphoswa icalculus.Imodeli yezinyo ke iye ichetywe kwaye iphawulwe ngegama lesigulana, inombolo kunye nomhla.Imodeli yodaka yaskenwa (inEos X5 CAD/CAM labhoratri iskena; Dentsply Sirona, PA, USA) ukwenza umfanekiso wedijithali omacala amathathu (3D) wemodeli yezinyo.Imilinganiselo efunekayo yenziwa kusetyenziswa i-AutoCAD version 2013 (AutoCAD; Autodesk, USA).Iiklinikhi zazingenalwazi ngamacala okulinga kunye nokulawula ngexesha lokulinganisa ukuphepha ukuchasana okungenangqiqo, kwaye ukuhlolwa kokuthembeka kwe-intra-investigator kwenziwa ngemilinganiselo ephindaphindiweyo ngumqhubi ofanayo ngeveki kamva ukujonga iimpazamo zokulinganisa.Impazamo yomlinganiselo oqikelelweyo yi-6%.
Iimpawu zomhlaba ezininzi zifunyenwe kwi-dental cast, kubandakanywa i-palatal suture ye-median, iindawo eziphakathi kwe-folding yesithathu ekhohlo nasekunene, kunye ne-cusps ye-maxillary canines ekhohlo nasekunene.Umgca othe nkqo ubaleka ukusuka kumanqaku aphakathi kwe-folds yesithathu ekhohlo nasekunene kunye ne-tubercles ye-maxillary canines ekhohlo nasekunene ukuya kwi-palatine suture ephakathi.Imilinganiselo ye-anterior-posterior yathathwa phakathi komgca we-canine we-bilateral kunye nomgca wesithathu we-fold fold ukuvavanya ukuchithwa kwe-canine (Imifanekiso 3, 4).
Fumana iimpawu zomhlaba kwimifanekiso eskeniweyo yeemodeli zamazinyo ukulinganisa ukurhoxa kwecanine.(Nye).Umthungo wepalatal ophakathi.(b, d).Iibhubhu ze-maxillary canines zasekhohlo nasekunene, ngokulandelanayo.(c, e).Imigca ehambelana neziphelo zangaphakathi zesithathu sasekhohlo nasekunene, ngokulandelelanayo.
Emva kokususwa kwikroba le-gingival, amaqela emicu yephepha elinezihluzo ezine eziqokelelwe kwindawo enye zafakwa kwiityhubhu ze-Eppendorf (Capp, Denmark) eziqulethe i-100 µl ye-phosphate-buffered saline.Iityhubhu ze-Eppendorf zitywinwe kwaye zibhalwe kwaye iisampuli zakhawuleza zafakwa kwi-3000 rpm kwimizuzu eyi-10 usebenzisa i-centrifuge (Hettich Universal 320R BC-HTX320; GMI, MN, USA) ukubuyisela iisampulu ze-GCF kwimicu.Iityhubhu ze-Eppendorf zigcinwe kwi--20 ° C de kube uhlalutyo lwe-biochemical.Uhlalutyo lwamanqanaba e-IL-1β lwenziwa kusetyenziswa i-enzyme-linked immunosorbent assay (ELISA; Cloud-Clone, Howe, USA).Ukuxinwa kwe-IL-1β kunqunywe ngokuthelekisa ubuninzi be-optical density (OD) yeesampuli ezifunyenweyo kunye nomgangatho oqhelekileyo kwaye ngokufanelekileyo kubalwe i-equation ye-linear regression of the curve standard.Ekugqibeleni, iziphumo zamanqanaba e-IL-1β zinikezelwa kwi-pg/ml/60 s25.I-flowchart yoyilo lophononongo iboniswe kuMfanekiso 5, oshwankathela inkqubo yokufunda.
Uhlalutyo lwamanani lwenziwa kusetyenziswa i-IBM SPSS yeWindows version 23.0 (IBM; Armonk, NY, USA).Zonke izinto eziguquguqukayo zamanani zivame ukuhanjiswa kwaye zithetha, ukuphambuka okusemgangathweni (SD) kunye ne-95% yexesha lokuzithemba (CI) zibalwe kwaye iimvavanyo zeparametric zisetyenzisiwe.Izinto eziguquguqukayo zobungakanani (i-canine retraction kunye ne-IL-1β level) zifaniswa phakathi kwamaqela amabini okufunda usebenzisa i-t-test yesampula ezimeleyo, ngelixa ukuthelekiswa phakathi kwe-laser kunye namacala okulawula kwiqela ngalinye kwenziwa ngokusebenzisa iimvavanyo ezidibeneyo.Ukuhoxiswa kwenja kunye namanqanaba e-IL-1β ngamaxesha ahlukeneyo kwiqela ngalinye kwafaniswa ngokwahlukileyo kusetyenziswa uhlalutyo lokulinganisa oluphindaphindiweyo lokuhluka okulandelwayo ngokuthelekiswa kweebini ezininzi usebenzisa i-Bonferroni-adjusted significance levels. Ukubaluleka kwamiselwa kwixabiso le-p <0.05. Ukubaluleka kwamiselwa kwixabiso le-p <0.05. Значимость была установлена ​​при значении p <0,05. Ukubaluleka kwamiselwa kwixabiso le-p <0.05.显着性设定为p值< 0.05.显着性设定为p值< 0.05. Значимость была установлена ​​на уровне p <0,05. Ukubaluleka kwamiselwa kwi p<0.05.
Ngexesha lophononongo, akukho sifundo siphumileyo nokuba ngexesha langaphambi kokungenelela okanye ngexesha eliseleyo lophononongo.Zonke izifundo ze-20 eziqeshwe ekuqaleni zagqiba ixesha lokufunda leeveki ezili-12 (izifundo ezili-10 kwiqela ngalinye).Ukuhamba kwesigulane kulo lonke ulingo kuboniswe kwi-Figure 6 usebenzisa i-flowchart ye-CONSORT.Idatha ye-demographic kwizifundo ezibhaliswe kwiQela A kunye ne-B zichazwe kwiThebhile 2. Kwakungekho ziganeko ze-prolapse kwiimodeli zokufunda, ezenziwa rhoqo kwiiveki ezintathu ukulinganisa ukuchithwa kwe-canine.Ukongeza, zonke iisampulu zeGCM ezifunyenweyo ziye zacutshungulwa ngononophelo kwaye zahlalutywa.
Isixa se-maxillary canine retraction kumaxesha ahlukeneyo achazwe kwiThebhile 3, malunga namaqela omabini A kunye no-B. KwiQela A, owona mgama mkhulu kakhulu (± SD) ohanjwe yi-maxillary canine iye yaxelwa kwiveki ye-3 ibe yi-1.18 (± 0.04) mm kwicala le-laser, kunye ne-0.85 (± 0.04) umehluko phakathi kwe-0. 1). Isixa se-maxillary canine retraction kumaxesha ahlukeneyo achazwe kwiThebhile 3, malunga namaqela omabini A kunye no-B. KwiQela A, owona mgama mkhulu kakhulu (± SD) ohanjwe yi-maxillary canine iye yaxelwa kwiveki ye-3 ibe yi-1.18 (± 0.04) mm kwicala le-laser, kunye ne-0.85 (± 0.04) umehluko phakathi kwe-0. 1). Величина ретракции верхнечелюстного клыка в разные моменты времени описана в таблице 3 для обеих групп А и В.В группе А наибольшее среднее расстояние (± SD), пройденное верхнечелюстным клыком на 3-й неделе, составляет 1,18 (± 0,04, ± 0,04 составляет) ,04) мм на стороне контроля, при этом разница между ними статистически значима (p <0,001). Isixa sokurhoxiswa kwe-canine ye-maxillary ngamaxesha ahlukeneyo achazwe kwiThebhile 3 kumaqela omabini A kunye no-B. Kwiqela A, umgama omde kakhulu (± SD) ohanjwe yi-maxillary canine ngeveki ye-3 yi-1.18 (± 0.04) mm kwicala le-laser kunye ne-0.85 (± 0.04) mm phakathi kwe-stat .Kumaqela A kunye no-B, iqondo le-maxillary canine retraction ngamaxesha ahlukeneyo achazwe kwiThebhile 3.在 A 组中，上颌尖牙移动的最大平均距离(± SD) 在第3 周报告為激光牙移动的最大平均距离(± SD) 在第3 周报告為激光侧為1.18 (± 0.04) i± 0.04) mm, ikhuphele0.000 ikhilomitha.间的差异具有统计学意义（p <0.001）。在 第 中 ， 上 颌 移动 的 最 大 距离 距离 在 第 3 周 报告 為激光 為移光 1.04 為1.04 ± 8/8 mm. ± 0.04 mm有 具有 具有 具有 具有 具有 具有 具有统计学意义（p <0.001）. В группе А максимальное среднее расстояние (± SD) движения клыков верхней челюсти на 3-й неделе составило 1,18 (± 0,04) м0, 5,04, 5 м на стороне контроля, разница между ними была Статистическая значимость (p <0,001). Kwiqela le-A, umgama ophezulu ophakathi (± SD) we-maxillary canine movement kwiveki ye-3 yayingu-1.18 (± 0.04) mm kwicala le-laser kunye ne-0.85 (± 0.04) mm kwicala lokulawula, umehluko phakathi kwabo babebalulekile ngokwezibalo (p <0.001). Nangona kunjalo, inani eliqhelekileyo lokunyakaza kwamazinyo liye lancipha ngeveki ye-6 kwi-laser kunye namacala okulawula, emva koko landa ngokuthe ngcembe emva kweeveki ze-9 kunye ne-12, kunye nenani lokuhamba kwezinyo liphezulu kakhulu kwicala le-laser xa kuthelekiswa necala lokulawula (p <0.001), kuwo onke amaxesha amanqaku. Nangona kunjalo, inani eliqhelekileyo lokunyakaza kwamazinyo liye lancipha ngeveki ye-6 kwi-laser kunye namacala okulawula, emva koko landa ngokuthe ngcembe emva kweeveki ze-9 kunye ne-12, kunye nenani lokuhamba kwezinyo liphezulu kakhulu kwicala le-laser xa kuthelekiswa necala lokulawula (p <0.001), kuwo onke amaxesha amanqaku.Nangona kunjalo, inani eliqhelekileyo lokufuduswa kwezinyo lehlile kwiveki yesi-6 kuwo omabini ama-laser kunye namacala olawulo, kwaye ke ngokuthe ngcembe lonyuka ngeeveki ze-9 kunye ne-12, kunye nesixa sokufuduswa kwezinyo siphezulu kakhulu kwicala le-laser.laser xa kuthelekiswa neqela lolawulo.стороны (p < 0,001) во все моменты времени. icala (p <0.001) ngamaxesha onke.然而，激光侧和实照侧的平均牙齿移动量在6 周下降，然后在第9 Izindlu kunye ne-12激光侧的牙齿移动量明显更高侧（p <0.001），在所有时间点.然而 , 激光 和 对照侧的 牙齿 移动量 在第 6 周 下降增加 , 与 对照 相比 , 的 移动量 明显 更 高侧 （p <0.001），在所有时间点. Однако среднее количество движений зубов на стороне лазера и контрольной стороне уменьшилось на 6-й неделе, а затем постепенно увели12зы 9 движений зубов на стороне лазера было значительно выше по сравнению с контрольной стороной (p <0,001) во все моменты времени. Nangona kunjalo, inani eliqhelekileyo lokunyakaza kwezinyo kwicala le-laser kunye necala lokulawula liye lancipha ngeveki ye-6 kwaye emva koko liye landa ngokuthe ngcembe emva kweeveki ze-9 kunye ne-12, kwaye inani leentshukumo zamazinyo kwicala le-laser laliphezulu kakhulu xa kuthelekiswa necala lokulawula (p <0.001) kuwo onke amanqaku ngexesha. Isixa esipheleleyo sokuhamba kwamazinyo (± SD) kwisithuba sokufunda iiveki ze-12 sasiphezulu kakhulu kwicala le-laser kunye ne-4.45 (± 0.13) mm, xa kuthelekiswa naleyo kwicala lokulawula elaliyi-3.16 (± 0.14) mm (p <0.001). Isixa esipheleleyo sokuhamba kwamazinyo (± SD) kwisithuba sokufunda iiveki ze-12 sasiphezulu kakhulu kwicala le-laser kunye ne-4.45 (± 0.13) mm, xa kuthelekiswa naleyo kwicala lokulawula elaliyi-3.16 (± 0.14) mm (p <0.001). Inkcazelo ngokuthe gabalala zубов (± SD) malunga 12-neдельный период исследования была значительно выше на стороне лазера – 4,43 (± 0, сыны) роной, которая составляла 3,16 (± 0,14) мм (p <0,001). Isixa esipheleleyo sokufuduka kwamazinyo (± SD) kwixesha le-12-iveki yokufunda yayiphezulu kakhulu kwicala le-laser, i-4.45 (± 0.13) mm, xa kuthelekiswa necala lokulawula, elaliyi-3.16 (± 0.14) mm (p <0.001).在為期12 周的研究期间，激光侧的牙齿移动总量(± SD) 显着更高，為4.45 (± 0.13) mm，而光侧的牙齿移动总量(± SD) 显着更高，為4.45 (± 0.13) mm，而光象 (0.4 mm) mm (3 ukuya ± 4 nge0 mm. 1).在為期12 周的研究期间，激光侧的牙齿的牙齿纻动总量(± SD) 显着更高，4.45 (± 0.13光侧的牙齿的牙齿纻动总量(± SD) 显着更高，為4.45 (± 0.13光公義) 4.45 (± 0.13光平) 4.45 (± 0.13 16) mm4 (± 0.16) (± 0.16) (± 0.16 (± 0.16) (± 0.16) (± 0.16) В течение 12-недельного периода исследования общее перемещение зубов (± SD) было значительно выше на стороне ,16 (± 0,14) мм в контрольной группе (p < 0,001). Ngethuba lokufunda kweeveki ze-12, ukuhamba kwamazinyo ngokupheleleyo (± SD) kwakuphezulu kakhulu kwicala le-laser kwi-4.45 (± 0.13) mm xa kuthelekiswa ne-3.16 (± 0.14) mm kwiqela lolawulo (p <0.001).
KwiQela B, iphethini efanayo naleyo ibonakaliswe kwiQela A ilandelwe, kunye nexabiso eliphezulu kakhulu lokuhamba kwamazinyo lirekhodwa kwicala le-laser, xa kuthelekiswa necala lokulawula kuwo onke amaxesha (p <0.001). KwiQela B, iphethini efanayo naleyo ibonakaliswe kwiQela A ilandelwe, kunye nexabiso eliphezulu kakhulu lokuhamba kwamazinyo lirekhodwa kwicala le-laser, xa kuthelekiswa necala lolawulo kuwo onke amaxesha (p <0.001). В группе B наблюдалась аналогичная картина, продемонстрированная в группе A, со значительно более высокими значениями друппе лазера, по сравнению с контрольной стороной во все моменты времени (p <0,001). Iqela B libonise ipateni efanayo kwiQela A, enexabiso eliphezulu kakhulu lokuhamba kwamazinyo erekhodiweyo kwicala le-laser xa kuthelekiswa necala lolawulo kuwo onke amaxesha (p <0.001).20 ）. <0.00 В группе B, по аналогии с группой A, зарегистрированные значения перемещения зубов были значительно выше на стороне лазения менты времени (p < 0,001). Kwiqela B, elifana neqela A, amaxabiso arekhodiweyo okuhamba kwamazinyo ayephezulu kakhulu kwicala le-laser xa kuthelekiswa necala lolawulo ngawo onke amaxesha (p <0.001).Emva kweeveki ze-3, ukunyakaza kwamazinyo amaninzi (± SD) kwabhalwa ngexabiso le-1.14 (± 0.04) mm kwicala le-laser kunye ne-0.87 (± 0.03) mm kwicala lokulawula.Ukushukuma kwamazinyo kuye kwehla kwiveki yesi-6 kwaye kwanda kancinci. Isixa esipheleleyo sokurhoxiswa kwe-canine (± SD) kwixesha le-12-iveki yokufunda kwi-laser kunye namacala olawulo, yi-4.35 (± 0.12) mm, kunye ne-3.10 (± 0.06) mm, ngokulandelanayo, kwaye umahluko phakathi kwabo wawubaluleke kakhulu ngokwezibalo (p <0.001). Isixa esipheleleyo sokurhoxiswa kwe-canine (± SD) kwixesha le-12-iveki yokufunda kwi-laser kunye namacala olawulo, yi-4.35 (± 0.12) mm, kunye ne-3.10 (± 0.06) mm, ngokulandelanayo, kwaye umahluko phakathi kwabo wawubaluleke kakhulu ngokwezibalo (p <0.001).Iyonke i-canine retraction (± SD) kwisithuba sokufunda iiveki ezili-12 kwilaser kunye namacala olawulo yayiyi-4.35 (± 0.12) mm kunye ne-3.10 (± 0.06) mm, ngokulandelelanayo, kwaye umahluko phakathi kwazo wawubalulekile ngokwezibalo.(р < 0,001). (p <0.001).在為期12 周的研究期间，激光侧和對照侧的犬齿回缩总量(± SD) 分别為4.35 (± 0.12) mm ± 0.12) mm ± 3.10 (6.10)异具有统计学意义(p <0.001).在 為期 12 周 的 研究 ， 激光 侧 和 对照侧 的回缩 总量 总量 (± sd) ± sd) ± 3/3/20 ± 30 (2 mm 30) (2 mm 60) (2 mm 40.3). mm , 之间 的 差异 具有 统计学 意义 (p <0.001). В течение 12-недельного периода исследования общая (± SD) ретракция клыка на стороне лазера и контрольной стороне составила 4,32, ± 0, ± 10, ± 10, ± 10 ответственно, и разница была статистически значимой (p <0,001). Ngethuba lokufunda kweeveki ze-12, i-totali (± SD) i-canine i-retraction kwicala le-laser kunye necala lokulawula laliyi-4.35 (± 0.12) mm kunye ne-3.10 (± 0.06) mm, ngokulandelanayo, kwaye umehluko wawubaluleke kakhulu (p <0.001). .Itheyibhile ye-4 ichaza ukuthelekiswa kweqondo lokurhoxiswa kwe-canine ngamaxesha ahlukeneyo phakathi kwe-laser kunye necala lokulawula kwiqela ngalinye lokufunda.
Nangona iqondo lokurhoxiswa kwe-canine nge-laser laliphezulu kwiqela le-A kuneqela B ngamaxesha onke, lo mahluko awuzange ucatshangelwe ngokwezibalo xa kuthelekiswa neqela B (p = 0.08-0.55).Ngokumalunga nokunyuka kwepesenti (± SD) kwi-canine retraction ephunyeziweyo ngeprotocol nganye, iprotocol esetyenziswe kwiqela A inyuke nge-40.78 (± 4.81)%, ngelixa iprotocol esetyenziswe kwiqela A inyuke nge-40 .22 (± 4.80) % kwiqela B. iprotocol yesicelo selaser efunyenweyo.Nangona kunjalo, nangona le pesenti yayiphezulu kancinane kwiqela A kuneqela B, umahluko phakathi kwabo wawungabalulekanga ngokweenkcukacha-manani (p = 0.82).Ukongezelela, kwafunyaniswa ukuba uhlobo lokuhamba kwamazinyo kumaqela omabini ayafana (umzobo 7).
Ukurhoxiswa kwe-Laser ye-canine esecaleni (mm) ngamaxesha ahlukeneyo kumaqela okufunda omabini ngexesha leeveki ezili-12.
Itheyibhile ye-5 ichaza amanqanaba e-IL-1β kumaqela A kunye no-B kuwo onke amaxesha alinganisiweyo kwi-laser kunye namacala okulawula.Kwiqela A, umahluko phakathi kwecala le-laser kunye necala lolawulo kwinqanaba lokuqala lalingabalulekanga kumaxabiso e-IL-1β (p = 0.56). Inqanaba eliphezulu le-IL-1β (± SD) lirekhodwe ngosuku lwe-7 kuzo zombini i-laser kunye namacala okulawula, kunye namaxabiso e-0.152 (± 0.004) pg/ml/60 s, kunye ne-0.127 (± 0.004) pg/ml/60 s, ngokulandelanayo, kunye ne-0.0 ephawulekayo ye-sta.0 p. Inqanaba eliphezulu le-IL-1β (± SD) lirekhodwe ngosuku lwe-7 kuzo zombini i-laser kunye namacala olawulo, kunye namaxabiso e-0.152 (± 0.004) pg/ml/60 s, kunye ne-0.127 (± 0.004) pg/ml/60 s, ngokulandelanayo, kunye ne-0.0 umahluko phakathi kwabo.Inqanaba eliphezulu le-IL-1β (± SD) lirekhodwe ngosuku lwe-7 kuzo zombini i-laser kunye namacala olawulo kunye namaxabiso e-0.152 (± 0.004) pg/mL/60 s kunye ne-0.127 (± 0.004) pg/mL./60 с соответственно, а разница между ними была статистически значимой (p <0,001). / 60 s, ngokulandelanayo, kwaye umehluko phakathi kwabo wawubaluleke kakhulu ngokwezibalo (p <0.001).在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD)，值為0.152 (± 0.004) pg/ml/60 s ne0.0g/s/60 s 和0.0g/sml/60 s ne0.0g/s/s之间的差异具有统计学意义（p <0.001）.在第7 天，激光侧和对照侧均记录到最高水平的IL-1β (± SD)，值為0.152 (± 0.004) pg/ml/60 s 和0.0g/4 p/60 s 和0.0g/4 p. ）.Ngomhla we-7, amanqanaba aphezulu e-IL-1β (± SD) abhalwe kuwo omabini ama-laser kunye namacala olawulo kunye namaxabiso e-0.152 (± 0.004) pg/mL/60 s kunye ne-0.127 (± 0.004) pg/mL./60 s.Разница между ними была статистически значимой (p <0,001). Umahluko phakathi kwabo wawubalulekile ngokwezibalo (p <0.001). Ukuncipha ngokuthe ngcembe kumanqanaba e-IL-1β kuye kwaxelwa emva koko, ngeentsuku ze-14 kunye ne-21, kuzo zombini i-laser kunye namacala okulawula, kunye namaxabiso kwicala le-laser liphezulu kakhulu kunelo kwicala lokulawula (p <0.001). Ukuncipha ngokuthe ngcembe kumanqanaba e-IL-1β kuye kwaxelwa emva koko, ngeentsuku ze-14 kunye ne-21, kuzo zombini i-laser kunye namacala olawulo, kunye namaxabiso kwicala le-laser liphezulu kakhulu kunalawo akwicala lolawulo (p <0.001). Inkcazo yenkqubo yezobuchwephesha ye-IL-1β kwi-14 kunye ne-21 yeentsuku как на стороне лазера, так и на стороне контролья, принязей тельно выше, чем на стороне контроля (p <0,001). Emva koko, ukuhla kancinci kumanqanaba e-IL-1β kwaxelwa kwiintsuku ze-14 kunye ne-21 kuzo zombini i-laser kunye namacala olawulo, kunye namaxabiso kwicala le-laser liphezulu kakhulu kunalawo akwicala lolawulo (p <0.001). .此后，在第14 天和第21 天，激光和对照侧的IL-1β 水平逐渐下降，激光侧的值显着高于对 <1(p0)此后，在第14 天和第21 天，激光和对照侧的IL-1β 水平逐渐下降，激光侧的值显着高于对 I-ИЛ-1β ibeka i-14 ukuya kweyi-21 i-ИЛ-1β ibeka isithuba se-14 ukuya kwi-21 ye-inkwenkwezi контроля (p < 0,001). Emva koko, ngomhla we-14 kunye neentsuku ze-21, amanqanaba e-IL-1β ayancipha ngokuthe ngcembe kwicala le-laser nakulawulo, ngelixa ixabiso kwicala le-laser laliphezulu kakhulu kunecala lokulawula (p <0.001).
Kwiqela B, iphethini efanayo yabonwa kwiqela A ngokubhekiselele kumanqanaba e-IL-1β, kunye nokungafani okuncinci okubonwa kwisiseko phakathi kwe-laser kunye namacala okulawula (p = 0.02). Emva kweentsuku ze-7, incopho yenqanaba le-IL-1β (± SD) yafikelelwa kumacala omabini, kunye ne-0.139 (± 0.004) pg/ml/60 s kwicala le-laser, kunye ne-0.122 (± 0.003) pg/ml/60 s kwicala lolawulo, kunye namaxabiso aphezulu kwi-1.0. Emva kweentsuku ze-7, incopho yenqanaba le-IL-1β (± SD) yafikelelwa kumacala omabini, kunye ne-0.139 (± 0.004) pg/ml/60 s kwicala le-laser, kunye ne-0.122 (± 0.003) pg/ml/60 s kwicala lolawulo, kunye ne-0.Emva kweentsuku ze-7, inqanaba eliphezulu le-IL-1β (± ukuphambuka okuqhelekileyo) kwafikelelwa kumacala omabini: 0.139 (± 0.004) pg/ml/60 s kwicala le-laser kunye ne-0.122 (± 0.003) pg/ml/60 s.на контрольной стороне, при этом значения на стороне лазера считались статистически более высокими (p <0,001). kwicala lolawulo, ngelixa amaxabiso kwicala le-laser ayethathwa ngokuba phezulu (p <0.001). 6制侧，激光侧的值在统计上更高（p <0.001）。 7 天 后 , 两 侧 达到 达到 il-1β 水平 (（±） , 激光 为 為 0.139 ± 0.004） pg/ml/6） pg/ml/6). 003） pg/ml/60 s 在侧 激光 激光 激光 激光 激光 激光 激光 ,值在统计上更高（p <0.001）。Emva kweentsuku ze-7, amanqanaba aphezulu e-IL-1β (± SD) afunyenwe kumacala omabini: 0.139 (± 0.004) pg / ml / 60 s kwicala le-laser kunye ne-0.122 (± 0.003) pg / ml / 60 s kwicala lokulawula., лазер Значения на стороне были статистически выше (p < 0,001). , Amaxabiso e-laser kwicala ngalinye ayephezulu ngokwezibalo (p <0.001).Amanqanaba e-IL-1β emva koko anciphisa ngokuthe ngcembe kumacala omabini kwiintsuku ze-14 kunye ne-21, kwaye amanqanaba arekhodiweyo kwicala le-laser ayephezulu kakhulu xa kuthelekiswa necala lokulawula kuzo zombini ixesha (p = 0.001-0.002).Ukuthelekiswa kwamanqanaba e-IL-1β ngamaxesha ahlukeneyo phakathi kwecala le-laser kunye necala lokulawula kwiqela ngalinye lokufunda lichazwe kwiThebhile 6.
Xa kuthelekiswa namanqanaba e-IL-1β phakathi kwamaqela amabini okufunda, ukungafani okungabalulekanga kubhalwe kwicala le-laser kwisiseko (p = 0.96). Ngeentsuku ze-7 kunye ne-14, iiyantlukwano ezibalulekileyo ngokwezibalo ziye zabhaliswa phakathi kwamacala e-laser kumaqela omabini, kunye namaxabiso aphezulu angamacala e-laser kwiQela A (p <0.001). Ngeentsuku ze-7 kunye ne-14, umahluko obalulekileyo ngokwezibalo ubhalisiwe phakathi kwamacala e-laser kuwo omabini amaqela, ngamaxabiso aphezulu angawamacala e-laser kwiQela A (p <0.001). На 7-й и 14 дни зарегистрированы статистически значимые различия между лазерными сторонами в обеих группах, причемысолезей ным сторонам в группе А (р < 0,001). Ngeentsuku ze-7 kunye ne-14, bekukho umahluko obonakalayo phakathi kwamacala e-laser kuwo omabini amaqela, ngamaxabiso aphezulu angawamacala e-laser kwiqela A (p <0.001).在第7 天和第14 天，两组激光侧的差异有统计学意义，A 组激光侧的值较高（p <0.001）. A На 7 ukuya 14 дни разница между двумя группами была статистически значимой на стороне лазера с более высокими значениями на стопроне 10 (10). Ngeentsuku ze-7 kunye ne-14, umahluko phakathi kwala maqela mabini wawubalulekile ngokwezibalo kwicala le-laser, kunye namaxabiso aphezulu kwicala le-laser kwiqela A (p <0.001).Emva kweentsuku ze-21, kwakungekho mmahluko omkhulu phakathi kwamaqela amabini (p = 0.26).Amanqanaba e-IL-1β kumaqela omabini anesimo esifanayo, afikelela kumlinganiselo ophezulu ngosuku lwe-7 kwaye ayancipha ngokuthe ngcembe kwi-14 kunye neentsuku ze-21 (umzobo 8).
Injongo yolu phononongo yayiphambili ukuvavanya nokuthelekisa umphumo we-LILR kwi-canine retraction usebenzisa i-protocol equka i-high frequency laser irradiation ngeentsuku ze-0, 3, 7, 14 kunye neeveki zonke ze-2 emva koko (iQela A) kunye nezigulane ezisanda kungena.bekukho ukukhumbula okumbalwa xa kuthelekiswa nerejimeni apho i-laser exposure yenziwa kwiiveki ezi-3 (iqela B).Ingaba iprotocol7,13,26 okanye iprotocol15,17,18 yeeveki ezi-3, zombini iiprotocol zichazwe kuncwadi.Ngokusekelwe kwiziphumo ezinikezelwe kuphononongo lwangoku, i-hypothesis engekhoyo ayizange yaliwe, kwaye ngokusetyenziswa kweeprothokholi ezimbini ezifundiweyo, amanani alinganayo eentshukumo zenja aphunyeziwe.
Uyilo lophononongo lwangoku lulingo lwekliniki olulawulwa ngokungahleliwe (RCT).Ii-RCT zithathwa njengomgangatho wegolide wokuvavanya iziphumo zongenelelo27.Ubuchule bokuqhekeka bomlomo busetyenziswe kwakhona, inzuzo ephambili kukuba ukuhluka phakathi kwezifundo kupheliswa, kunye nesigulane ngasinye sisebenza njengomlawuli wabo, ngaloo ndlela sinciphisa inani labathathi-nxaxheba abafunekayo.
Zonke izifundo ezibandakanyiweyo kwisifundo zifuna ukukhutshwa kwe-maxillary yokuqala ye-premolar elandelwa yi-canine retraction njengenxalenye yonyango lwe-orthodontic.Ekubeni i-extraction inokutshintsha izinga le-RTM ngokunyusa umsebenzi wabamakishi abavuthayo, nto leyo inokufihla umphumo we-LILT kwaye inike ukufundwa kobuxoki kwamanqanaba e-IL-1β xa usebenzisa i-laser, unyango lwe-extraction lwenziwa ngaphambi kokuba unyango lunike umphumo omuhle.Isisombululo sesokhethi yokuphilisa sinika ixesha elaneleyo kwaye soyise iziphumo zeziganeko zokukhawulezisa zengingqi28.Olu lumkiso luye lwathathwa nangababhali abathile, i-11 ephanda umphumo we-LILT kwizinga le-OTM ngexesha lokurhoxiswa kwezinja ngokulinganisa amanqanaba e-biomarkers afana ne-IL-1β kunye nokuguqula ukukhula kwe-β1 (TGF-β1) kwi-GCF.
Uhlobo lwe-laser olusetyenzisiweyo kolu cwaningo lwaluyi-laser diode semiconductor laser esetyenziswe kwi-980 nm ngokweengcebiso zomenzi we-biostimulation efanelekileyo.Oku kunokuchazwa yinyaniso yokuba ixesha elide i-laser wavelength (650-1200 nm), i-tishu ingena nzulu29.Nangona kunjalo, le wavelength ecetyiswayo isetyenziswe kwezinye izifundo ezininzi, ukuvelisa iziphumo ezilungileyo zokukhawuleza kwe-8.30 kunye nemiphumo emibi ye-14.
Enye into ebalulekileyo echaphazela ukusebenza konyango lwe-LILI kunye ne-biostimulation yi-dose okanye ubuninzi bamandla.Xa kuphononongwa uncwadi, kwafunyaniswa ukuba kukho i-heterogeneity enkulu kwidosi ye-LILI yamandla ukukhawulezisa i-GTM.Abanye ababhali babika iziphumo ezilungileyo xa besebenzisa amandla aphantsi kwamandla ukusuka kwi-0.7131, 532.33, 7.514 ukuya kwi-8 J / cm234.35, ngelixa abanye abaphandi baxela umphumo we-LILR kwizinga le-GTM kuxinzelelo lwamandla aphezulu, umzekelo, i-25 J / cm2.cm27.36.Kulo msebenzi wangoku, umthamo wezinga eliphantsi le-laser energy ye-8 J / cm2 lihanjiswe ngokuvezwa okukodwa kwingcambu ye-maxillary canine imizuzwana ye-8 usebenzisa i-flat top tip ukusabalalisa indawo ye-1 cm2.Kukho ulungelelwaniso oluthe ngqo phakathi kobukhulu bomqadi kunye nobunzulu bokungena kwe-laser, nto leyo eqinisekisa ukusetyenziswa kwezandla eziphezulu eziphezulu kolu phononongo29,37.Iprotocol yesicelo esisodwa esinobukhulu obukhulu bendawo ye-boam yenziwa ngokulungelelanisa kunye nokulungelelaniswa kwe-8 kunye ne-canine retraction 38.
I-IL-1β iyaziwa ngokuba yi-cytokine ebalulekileyo ye-pro-inflammatory ekuqaleni kwe-OTM kwaye ithathwa njengophawu lokubuyisela amathambo.Ngoko ke, amanqanaba e-IL-1β ahlolwe yi-laser kwizifundo ezininzi11,39,40 ngenzame yokumisela ukulungelelaniswa kwabo.Kulingo lwangoku, amanqanaba e-IL-1β kwi-GCF ahlolwe kumacala okulinga kunye nokulawula kwiqela ngalinye ngokusebenzisa iirejimeni ezimbini ezahlukeneyo ze-LILI kwiintsuku ze-0, 7, 14, kunye ne-21.
Kuphononongo lwangoku, ukuhoxiswa kwe-canine nge-laser kumaqela A kunye ne-B kwakuphezulu kakhulu kuneqela lokulawula kuwo onke amanqaku avavanyiweyo, ukunyuka kweveki ye-3, ukuhla kweveki enye ngeveki ye-6, kwaye ngokukhawuleza kwanda ukuya kwiveki ye-12..I-Peak canine movement ephawulwe ngeveki ye-3 inokuchazwa ngomphumo wokufuduka kwamazinyo okuqala, kubandakanywa: ukufuduka kweengcambu kwi-PDL, ukukhubazeka kwamathambo ngenxa ye-flexure kunye ne-creep, kunye nokunyanzeliswa koxinzelelo lwezinyo ngenxa yokuthambekela kwe-conical socket I-Plane effect 41. Ukongezelela, kuye kwafunyaniswa ukuba zonke iinkqubo ze-biological ezisebenzayo zihlala zikhawuleza kwi-deform position xa i-deform i-deform.Ukunciphisa okulandelayo okubonwayo phakathi kwe-3 kunye neeveki ze-6, mhlawumbi ngenxa yexesha lokulibaziseka elinokuthi lihluke kwii-2 ukuya kwiiveki ze-10, lixesha lokuphazamiseka kwe-PDL ekhuphayo kwaye isuse ithambo elikufutshane nommandla wokutyumza, ukuvumela ukunyakaza kwamathambo.amazinyo.Enye into enegalelo kule ngqalelo inokuthi i-oxygen fibers, i-collagen fibers, kunye nokulungiswa kwamathambo e-alveolar kwicala loxinzelelo kunokunciphisa izinga lokuhamba kwamazinyo.Iipateni ezifanayo zokunyakaza kwamazinyo zifunyenwe kwi-cleft study45 ngokuthelekisa imiphumo ye-LILI kunye ne-corticotomy kwizinga lokubuyiswa kwe-canine, baqaphela ukuba ukunyakaza kwamazinyo kwakukhulu kwiiveki ze-2 kunye ne-5, kulandelwa ukuhla ngokukhawuleza kwi-2 kunye ne-5 iiveki.iveki.Oku akuzange kuxelwe kwicala le-laser kwiveki yesi-7, kodwa hayi kwicala le-corticotomy.
Ingxelo yepesenti yokwanda kwepesenti kumgama wokuhamba kwe-maxillary canine ukusuka kwicala le-laser yayingu-40.78% kwiqela A kunye ne-40.22% kwiqela B. Ukwanda okubonakalayo kokuhamba kwamazinyo okuhamba kunye nokusetyenziswa kwe-laser kunokuchazwa kwinqanaba leselula ngokufunxwa kwamandla e-laser nge-photoreceptors kwi-chainrial yokuthutha i-electron kwi-membrane yokuthutha.Esi siphumo sikhokelela ekusebenzeni kwexesha elifutshane lekhonkco lokuphefumula, elikhokelela kwi-phosphorylation ye-oxidative kunye noshintsho kwimeko ye-redox ye-mitochondria yeselula kunye ne-cytoplasm.Emva koko, amandla okuqhuba iseli ayanda ngokunyusa ukunikezelwa kwe-ATP.Ukongezelela, kukho ukwanda kwamandla e-membrane ye-mitochondrial, i-alkalization ye-cytoplasm, kunye ne-synthesis ye-nucleic acids.Ekubeni i-ATP yaziwa ngokuba yimali yamandla eeseli, i-LILI inegalelo ekusebenzeni okuqhelekileyo kweeseli ngokudala indawo efanelekileyo yokunyakaza kwamazinyo46.Ngaloo ndlela, ukusuka kwiziphumo zethu, sinokugqiba ukuba ukusetyenziswa kwe-LILT njengento yokuncedisa unyango lwe-orthodontic kunokukhawuleza ukukhawuleza kwe-OTM kungakhathaliseki ukuba isetyenziswe rhoqo njenge-regimen kwiqela A (ngeentsuku ze-0, 3, 7, 14 kunye nosuku ngalunye).emva kweentsuku ze-2) iiveki), okanye ukuba isetyenziswe ngaphantsi kweqela B (rhoqo kwiiveki ezi-3), ngoko ke, i-hypothesis engekhoyo ayizange yaliwe.
Iziphumo ezifanayo ezikhawulezayo ezifanayo kwiiprothokholi ezimbini ezivavanyiweyo ze-LILT ezixelwe kolu phononongo zisenokuba ngenxa yobukho bomda wokuvula iselula apho ukwanda kokusebenza kweselula kunye nokuvezwa kwe-LILT kwenzeka ekuqaleni, kodwa emva koko ukuvezwa okuphindaphindiweyo (njengeqela A), ngenxa yokugcwala kwe-biological reactions akuyi kukhokelela ekuqhubekeni kusebenze.Ke, sinokucinga ukuba iziphumo ze-LLLT kwinqanaba leeselula azikwazi ukuqokeleleka.Ngokumalunga nobudlelwane phakathi kwenqanaba lamandla kunye nesantya sokuhamba kwamazinyo, ingqikelelo ye-biosaturation ichazwe ngaphambili.
Emva kokuphonononga uncwadi olukhoyo, sithelekise ukwanda kwe-1.4-fold (40-41%) kwi-WTM efunyenwe kwisifundo sethu sisebenzisa iiprothokholi ezimbini ze-laser kunye neziphumo zezinye iingxelo ezininzi.Olunye uphononongo luchaze iziphumo ezifanayo11,30,48,49 ngelixa ezinye zichaze amaxabiso asezantsi okukhawulezisa asetyenziswa kusetyenziswa i-LILI7,18,32,40.Kwelinye icala, amaxabiso okukhawulezisa aphezulu kakhulu kunezo zichazwe kwiimvavanyo zangoku, ukusuka 1.65 × 17 phantse 2x OTM15, 34, 39, 50, enokuthi inxulumene ezinye zazo Sebenzisa iibrayisi zokutshixa ngaphandle kongquzulwano 15. Lo mahluko kwiziphumo ezipapashiweyo kwiincwadi zisenokuba ngenxa yeendlela ezahlukeneyo zokusebenzisa i-laser yonyango, i-exposure yexesha, i-exposure ye-laser yonyango ., eyenza uthelekiso oluthe ngqo phakathi kwezifundo ezahlukeneyo kube nzima kakhulu..Nangona kunjalo, kuye kwaphawulwa ukuba ukuxinwa kwamandla aphantsi (umzekelo, i-2.5, i-5 kunye ne-8 J / cm2) ibonelela ngobuchule obungcono bokukhawulezisa xa kuthelekiswa nokuxinana kwamandla aphezulu, kuyafaneleka ukuba uqaphele ukuba iidosi ezisetyenziswe kwiimvavanyo zethu ziyi-8 J / cm2.cm2.
Ukutolikwa kwamanqanaba e-IL-1β kwi-distal cleft (icala loxinzelelo) emva kokuhlalutya kweesampuli ze-GCF ezifunyenweyo zibonise ukunyuka okuphawulekayo kwisiseko (oko kukuthi i-peak) ngosuku lwe-7 lulandelwa ukuhla kancinci ukuya kwisiseko.kwiipaneli ze-A kunye ne-B, kwicala le-laser kunye nakwicala lokulawula.Oku kunokuchazwa yinyaniso yokuba isigaba sokuqala se-OTM ngokuqhelekileyo sihamba kunye nokunyuka komsebenzi we-osteoclast.I-IL-1β nayo ithathwa njengeyona nto ibonakalayo yokumakisha ehambelana ne-bone resorption, kwaye i-IL-1β ibonakaliso iye yabikwa ukuba yanda ngamandla kwaye emva koko iyancipha kwizifundo ezininzi11,20,51.
Ukongezelela, amanqanaba e-IL-1β ayephezulu kwicala le-laser xa kuthelekiswa neqela lolawulo kuwo omabini amaqela okufunda kuwo onke amaxesha alinganisiweyo ngaphandle kwesiseko, kwaye kukho umehluko ophawulekayo phakathi kwabo.Oku kubonisa ukuba i-laser irradiation ephantsi kakhulu ibangele impendulo ephuculweyo yebhayoloji kwizicubu ze-periodontal kwicala lovavanyo ngendlela yokuvuselela umsebenzi we-osteoclast kwicala elicinezelweyo ngexesha lokuhamba kwamazinyo.Le mpembelelo ye-LLLT kumanqanaba e-IL-1β ibonakaliswe kwizifundo ezahlukeneyo11,39,40.
Xa kuthelekiswa namanqanaba e-laser-side IL-1β kumaqela amabini okufunda, amanqanaba ayephezulu ngokwezibalo kwiqela A xa kuthelekiswa neqela B kwiintsuku ze-7 kunye ne-14. Oku kunokuchazwa ngenani elikhulu lokuvezwa kwe-laser irradiation kwiqela le-A ngexesha leentsuku ze-21 zokujonga, apho i-irradiation yenziwa ngeentsuku ze-0, 3, 7, kunye ne-1 kuphela, kunye ne-14 yomlilo, kunye no-14 kuphela, kunye ne-14 yomlilo, kunye ne-14 B. Amanqanaba e-β ayephezulu ngokwezibalo kwicala le-laser kwiqela A, lo mahluko wezibalo awuzange uboniswe ngokweklinikhi kwiqondo lokuhlehla kwizinja xa kuthelekiswa necala le-laser kwiqela B, njengoko kwakungekho kubaluleka kwamanani.Kumaqela A kunye no-B, ukungafani okuxeliweyo kwi-canine retraction phakathi kwamacala e-laser ngokwenene kubangele umlinganiselo ofanayo wokuhamba kwe-canine.Ngoko ke, sinokuthi ukungafani kwezibalo akuchazi ukubaluleka kweklinikhi.
Unyango lwe-laser oluphantsi, xa lusetyenziswe kunye neeparitha ezisetyenzisiweyo kolu cwaningo, lunokukhawuleza ngokukhawuleza ukunyakaza kwamazinyo e-orthodontic malunga namaxesha angama-1.4, nokuba isetyenziswe kwi-frequency ephezulu okanye ephantsi, ehambelana nokulandelwa rhoqo, mhlawumbi.ifaneleke ngakumbi izigulane.
Ukunyuka kwezinyo lokuhamba nge-orthodontic ngexesha le-LILI lihamba kunye nokunyuka kwinqanaba le-interleukin-1β kwicala elixinzelelweyo, elibonisa ukuba ukusetyenziswa kwe-LILI kubangela inkqubo ephuculweyo yokuguqulwa kwamathambo.
Iiseti zedatha ezisetyenzisiweyo kunye/okanye ezihlalutyiweyo kuphononongo lwangoku ziyafumaneka kubabhali abachaphazelekayo ngesicelo esinengqiqo.
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