"Musambofa makakahadzika kuti boka diki revagari vane pfungwa dzakadzama uye vakazvipira rinogona kushandura nyika. Kutaura zvazviri, ndiro rega riripo."
Chinangwa chaCureus ndechekuchinja nzira yekare yekuburitsa zvinyorwa zvekurapa, umo kutumirwa kweongororo kunogona kudhura, kuoma, uye kutora nguva.
Plasma/prp yakapfuma mumaplatelet, kugadzurwa kwenyama, kusimbiswa kwemaplatelet, kurapwa kweglucose proliferative, maplatelet, kurapwa kwekuwedzera
Taura chinyorwa chino seizvi: Harrison TE, Bowler J, Reeves K, et al. (Chivabvu 17, 2022) Mhedzisiro yeglucose pahuwandu hwemaplatelet uye vhoriyamu: mhedzisiro yemushonga unovandudza muviri. Cure 14(5): e25081. doi:10.7759/cureus.25081
Plasma yakapfuma muropa (PRP) uye mhinduro dzeglucose dzakanyanya kushandiswa pakuisa jekiseni mumishonga inovandudza masero, dzimwe nguva pamwe chete. Mhedzisiro yeglucose yakanyanya kushandiswa pakuisa platelet muropa uye pakuita kuti ropa rishande haisati yambotaurwa. Takaedza mhedzisiro yehuwandu hweglucose hwakakwira pahuwandu hweplatelet nemasero eropa, pamwe nehuwandu hwemasero muPRP neropa rose (WB). Kudzikira kwehuwandu hweplatelet kwakaitika nekukurumidza nemusanganiswa wese weglucose wakasanganiswa nePRP kana ropa rose, zvichienderana nekuisa glucose muropa rose. Mushure meminiti yekutanga, huwandu hwemaplatelet hwakaramba hwakagadzikana, zvichiratidza kuti maplatelet akasara aifanira kugadzika nekukurumidza kusvika pahuwandu hwakanyanya (>2000 mOsm). Mushure meminiti yekutanga, huwandu hwemaplatelet hwakaramba hwakagadzikana, zvichiratidza kuti maplatelet akasara aifanira kugadzika nekukurumidza kusvika pahuwandu hwakanyanya (>2000 mOsm). После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструю аккомодацию остаточных тромбильным мОсм) гипертонуса. Mushure meminiti yekutanga, huwandu hwemaplatelet hwakaramba hwakagadzikana, zvichiratidza kuti maplatelet akasara akakurumidza kugadzikana zvakanyanya (>2000 mOsm).第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端（> 2000 mOsm漸。2000 mOsm）高渗状态. После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструю адаптацию остаточных тромбильным мОсм) гиперосмолярному состоянию. Mushure meminiti yekutanga, huwandu hwemaplatelet hwakaramba hwakagadzikana, zvichiratidza kukurumidza kugadziriswa kwemaplatelet akasara kusvika padanho repamusoro (>2000 mOsm) hyperosmolar.Kuwanda kweglucose ne25% zvichikwira kwakaita kuti pave nekuwedzera kukuru kwehuwandu hwemaplatelet (MPV), zvichiratidza kuti platelet yatanga kushanda. Zvidzidzo zvakawanda zvinodiwa kuti zvionekwe kana platelet ichibviswa kana kuti kuiswa kweglucose muropa kunoitika uye kana kuiswa kweglucose muropa chete kana pamwe chete nePRP kunogona kuwedzera rubatsiro rwekiriniki.
Mumakore ekuma1950, chiremba wekuAmerica George Hackett akawana kuti aigona kubvisa zvachose marwadzo emajoini nemusana muvarwere vazhinji nekuisa mushonga wekuwedzera mumasero nemarunda. Kuedza kwake patsuro kwakaratidza kuti kurapwa kwacho, kwaakadana kuti kurapa kwekuwedzera, kwakaita kuti masero awedzere kukura uye asimbiswe. Zvidzidzo zveHistological zvakasimbisa kuti collagen itsva inogadzirwa panguva iyi [1].
Mumakumi emakore ekutanga, nzira dzakasiyana-siyana dzekugovera dzakaedzwa. Pakazosvika makore ekuma1990, vazhinji vevashandi vekurapa vakaona kuwanda kweglucose senzira yakachengeteka uye inoshanda zvakanyanya. Zvisinei, mashandiro ekushanda kwayo haasati ajeka.
Zvidzidzo zvishoma zvekiriniki zvakaitwa muzana remakore rechi20 mushure mebasa raHackett. Zvisinei, mumakore ekuma2000 kwakave nekufarira kwakawedzerwa uye miedzo yakawanda yekiriniki yekurapa kwakabudirira yakapedzwa yekurapa marwadzo emusana [2], osteoarthritis yemabvi [3], uye lateral epicondylitis [4].
Kugadziriswazve kwenyama kunoda kuti masero ekutanga ashande pamwe chete. Nokudaro, kuwanda kweglucose kunofanira kukonzera kufamba, kudzokororwa, uye kupatsanurwa kwemasero ekutanga. Tinofungidzira kuti maplatelet anogona kushanda senhume uye kuti kuwanda kweglucose kunogona kukonzera maplatelet kuburitsa ma cytokines nezvinhu zvinokura, nokudaro zvichikurudzira maitiro ekuvandudza, kunyanya kufamba kwemasero ekutanga kunzvimbo dzine kuwanda kweglucose.
Kushanda kwemaplatelet nguva dzose kunotanga kuwedzera kwecalcium mukati memasero [5]. Liu nevamwe vake muna 2008 vakaratidza kuti huwandu hwakawanda hweglucose hunowedzera kushanda kwe transient receptor potential canonical type 6 (TRPC6) channels mu plasma membrane, izvo zvinotungamira mukuuya kwecalcium ions mumaplatelet [6]. Imwe ongororo yakaratidza kuti kuiswa kwe microtubule marginal zone kuma calcium ions kunokonzera kuzorora, kuwedzera, uye kushanduka kwe marginal zone, izvo zvinokonzeresa shanduko muchimiro kubva pa disc kuenda ku spherical, zvichikonzera mean platelet volume (MPV) [7].
Fungidziro yedu muchidzidzo ichi ndeyekuti kuwanda kwemaplatelet muglucose kunokanganisa nzvimbo iri pakati pe microtubule nemukati memasero, zvichikonzera kuwedzera kweMPV.
Vese vakatora chikamu vakasaina fomu rekubvumirana mushure mekunge ruzivo rwechidzidzo rwatsanangurwa uye vasati vagamuchira mienzaniso. Muchidzidzo ichi, sampuli dzePRP chete dzine hematocrit inopfuura 2% dzakashandiswa kuitira kuti erythrocyte (erythrocyte) ive yakawanda uye avhareji yehuwandu hwemasero matsvuku eropa (MCV) zvigone kuiswa kuti zvienzaniswe.
Chidzidzo ichi chakaitwa muzvikamu zvina, chikamu chekutanga chaive PRP uye zvikamu zvakasara zvaive ropa rose (Tafura 1). Sezvakatsanangurwa kare [8], masimba ese epakati pepakati (RCF, g-force) akaverengerwa kubva pakati (Rmid, in cm) yekoramu yeropa iri musirinji yepakati pepakati. Takasarudza kushandisa MPV sechiratidzo chekunzwa kweplatelet uye huwandu hweplatelet sechiratidzo chekuyerwa kweplatelet, zvese izvi zvinogona kuyerwa zviri nyore pane standard hematology analyzers.
Muchikamu chekutanga, vanhu makumi mana nevanomwe vakapa sampuro dzeropa—chubhu imwe chete ye ethylenediaminetetraacetic acid (EDTA) uye sampuro imwe chete yeropa rose rePRP (risingasanganisike ne sodium citrate (NaCl, 3%)) (Tafura 1). Isa rocker muchubhu nekukasika. Kuverengwa kweropa kwakazara (CBC) kwakaitwa pa EDTA sampuro muzvikamu zvitatu, uye sampuro dzeNaCl dzakaongororwa muzvikamu zvitatu kuti dziongororwe neCBC, uye PRP yakazogadzirwa nenzira dzakasiyana-siyana dzakatsanangurwa pamusoro apa [8]. Sampuro dzese dzePRP dzakagadzirwa ne centrifugation pa 900–1000 g. Sanganisa sampuro yega yega yePRP pamuchina wekusanganisa vortex kwemasekondi mashanu kusvika gumi, wobva wagovanisa ma aliquot mashanu e 0.5 ml mumachubhu.
Kuti vaongorore mhedzisiro yekuonekwa kweplatelet pahuwandu hweglucose hwakakwira, huwandu hwakaenzana (0.5 ml) hwe0%, 5%, 12.5%, 25%, uye 50% glucose mumvura hwakasanganiswa nemaplatelet samples kuti pave nehuwandu hwe0%, 2.5% 6.25%, 12.5% ​​​​uye 25% yeglucose musanganiswa uye kusanganisa machubhu pa test tube shaker kwemaminitsi gumi nemashanu. TAC yemusanganiswa wega wega yakaongororwa katatu mushure memaminitsi gumi nemashanu. Kuwanda kwemaplatelet (PLT), RBC count, MCV, uye MPV zvakaverengerwa paavhareji yechubhu yega yega, uye avhareji yehuwandu hwemaplatelet, RBC count, MCV, uye MPV zvakaverengerwa kune ese maPRP samples.
Mushure mekunge chikamu chekutanga chekuunganidza data chapera, takaona kuwedzera kukuru kwehuwandu hwemaplatelet mumaplatelet ePRP mushure mekuwedzerwa kweD50W. Maplatelet ePRP haangomiriri maplatelet ese ari muropa, uye PRP medium yakasiyana neWB medium. Saka, takasarudza kuita chikamu chechipiri chekuedza mhedzisiro yekuwedzera D50W muropa rose.
Pachikamu chechipiri, takasarudza saizi yesampuro ye30 zvichibva pane zvakabuda muchikamu chekutanga, sezvakatsanangurwa muchikamu cheOngororo. Muchikamu chino, vanhu makumi maviri vakazvidira vakapa sampuro dzeropa (Tafura 1). Ropa rose (1.8 ml) rakatorwa musirinji ye3 ml uye rakaiswa mushonga we0.2 ml ye40% NaCl. Sirinji yese yeropa yakasanganiswa kwemasekondi mashanu nemuchina wevortex uye CBC yakaongororwa katatu. Mushure mekuongorora, ropa rakaiswa mushonga wevasingapindi ropa rakawedzerwa ku2 ml ye50% glucose musirinji ye5 ml (huwandu hwekupedzisira hweglucose hwaive 25% (D25) uye hwakaiswa muchubhu yekuzunguza kwemaminitsi makumi matatu. Mushure memaminitsi makumi matatu, D25/CBC musirinji dzeWB dzakaongororwa katatu. Kuwanda kwemaplatelet, RBC count, MCV, uye MPV pasirinji imwe neimwe zvakaverengerwa, uye avhareji yePLT, RBC count, MCV, uye MPV zvakaverengerwa pasampuro yega yega isati yawedzerwa glucose uye mushure mekuwedzera.
Nekuti maplatelet muropa rose anowanzo sangana neglucose yakawanda panguva yekurapwa neglucose yakawanda nekuda kwekubaiwa jekiseni zvishoma, uye hazviwanzo sanganisirwa PRP neglucose yakawanda usati wabayiwa jekiseni, takasarudza kudzidza nezveglucose yakawanda pamwe chete neWB muChikamu 1. Danho rechitatu nerechina. Padanho rega rega, vanhu makumi maviri vakazvidira vakapa 7-8 ml yeACD-A (acid ine trisodium citrate (22.0 g/l), citric acid (8.0 g/l) uye glucose (24.5 g/l), solution dextrose citrate) yemishonga inorwisa ropa (Tafura 1). Musanganiswa weglucose inopfuura 12.5% ​​chete ndiwo wakashandiswa kuona chiyero chepakati chakabatana nekuwedzera kweMPV. Padanho rechitatu, 1 ml yeropa inoiswa muchubhu yekuyedza. Wobva wasanganisa ropa pamuchina wekusanganisa mvura kwemasekondi gumi nekuwedzera 1 ml ye30% glucose, 40% glucose, kana 50% glucose muchubhu kuti uwane huwandu hwekupedzisira hweglucose hwe15%, 20%, uye 25%, zvichiteerana. Sampuli dzeropa reglucose dzakaongororwa kuti dzionekwe neCBC mushure mekusanganiswa uye dzakadzokororwa kwemaminitsi maviri ega ega kwemaminetsi makumi matatu.
Munguva yekusanganisa kwekutanga, kuwedzerwa kwe1:1 hypertonic glucose neWB kana PRP kunoisa maplatelet muhuwandu huri pamusoro pe25% kwemasekonzi akati wandei. Mudanho rechina, kuti tiongorore mhedzisiro yehypertonic glucose nehuwandu hushoma hwekutanga uye kuyedza muganho wepamusoro wemhedzisiro yeglucose, takawedzera huwandu hushoma hweropa kuD25W kana D50W. Isa 1 ml yeD25W kana D50W muchubhu uye wedzera 0.2 ml yeWB uku uchivhomora sampuli kwemasekonzi gumi. Muzviitiko izvi, ropa rakaiswa muhuwandu hweglucose pahuwandu hunenge 20% pamusoro pehuwandu hwekupedzisira, pane 50% pamusoro pehuwandu hwekupedzisira semuChikamu chechitatu, zvichikonzera huwandu hwekupedzisira hweglucose hwe20.8% ne41.6%. Samples dzakasanganiswa dzakaongororwa panguva imwe chete semudanho rechitatu.
Mudanho rekutanga rechikamu chega chega che glucose dilution, sampuli makumi matatu dzakatorwa sezvo iyi yaive saizi yakakodzera yesampuro yekudzidza kwekuyedza [9]. Pakupera kwechikamu chega chega (kusanganisira chikamu chekutanga), ongorora kukwana kwehukuru hwesampuro uchishandisa fomura yakashandiswa kuona saizi yesampuro inodiwa kufungidzira avhareji yemhedzisiro inoenderera mberi muhuwandu humwe. Fomura n = Z2 x SD2 /E2. Muequation iyi, Z iZ-score, SD istandard deviation, uye E idikanwi yekukanganisa [10]. Alpha yedu i0.05, iyo inoenderana neZ value ye1.96, uye tinotarisira kukanganisa kwe5 (muzana). Saka tinogadzirisa kuti n = (1.962 x SD2)/52. Mhedzisiro yakaratidza kuti saizi yesampuro inodiwa padanho rega rega yaive diki pane nhamba chaiyo yakaunganidzwa.
Munguva yechikamu 1, 3 ne4 uchishandisa huwandu hweglucose hunopfuura humwe chete, mhedzisiro yehuwandu hweglucose hwakasiyana yakaongororwa nekuenzanisa shanduko yechikamu pakati penguva 0 nenguva yega yega inotevera (chikamu 1 pamaminitsi gumi nemashanu, chikamu 3 pamaminitsi gumi nemashanu). uye mana pamasekondi gumi nemashanu, wozotevera maminitsi maviri ega ega.) Mwero yekuchinja yenguva yega yega yakaenzaniswa uchishandisa Mann-Whitney U-test nekuti data harina kutevera kugoverwa kwakajairika sezvakatsanangurwa neShapiro-Wilk normality test. Sezvo ongororo ye1-to-1 yemapoka akati wandei (mashanu) yakaitwa munhanho yekutanga, yechitatu neyechina (mashanu ese), kugadziriswa kweBonferroni kwakaitwa kugadzirisa alpha value yaidiwa kuita ≤0.01 asi kwete ≤0.05.
Kuderedzwa kwehuwandu hwemaplatelet nekuwedzera kwehuwandu hwe hypertonic dextrose uye kuwedzera kweMPV mumaplatelet ePRP pa >12.5% ​​dextrose concentration: Huwandu hwemaplatelet ePRP hwakawedzera kubva pahuwandu humwe kusvika pashanu zvichienzaniswa neropa rese rekutanga, zvichisiyana nenzira (isina kuratidzwa). Kuderedzwa kwehuwandu hwemaplatelet nekuwedzera kwehuwandu hwedextrose yehypertonic uye kuwedzera kweMPV mumaplatelet ePRP pahuwandu hwedextrose hwe>12.5%: huwandu hwemaplatelet ePRP hwakawedzera kubva pahuwandu humwe kusvika kushanu zvichienzaniswa neropa rese rekutanga, zvichisiyana nenzira (isina kuratidzwa). Уменьшение количества тромбоцитов при всех концентрациях гипертонической декстрозы и увеличение MPV в тромбоцитах PRP прицентрациях гипертонической декстрозы и увеличение MPV в тромбоцитах PRP прицентрациях приконцикыт% количество тромбоцитов PRP увеличилось в 1-5 раз показано в зависимости от метода (не показано). Kuderera kwehuwandu hwemaplatelet pahuwandu hwese hwe hypertonic dextrose uye kuwedzera kweMPV mumaplatelet ePRP pahuwandu hwe >12.5% ​​​​hwe dextrose: Huwandu hwemaplatelet ePRP hwakawedzera ka1-5 zvichienzaniswa neropa rese rekutanga, zvichienderana nenzira (isina kuratidzwa). ).在> 12.5% ​​的葡萄糖浓度下，所有浓度的高渗葡萄糖降低血小板计数，PRP 血小板中MPV增加：与基线全血相比，PRP 血小板计数从浓度的1 倍上升到5 倍，因方法而异（未漉。 Pahuwandu hweglucose huri >12.5% ​​​​, huwandu hwakawanda hweglucose hunoderedza huwandu hweropa, PRP blood MPV inowedzera: zvichienzaniswa ne 与基线全血, huwandu hweropa rePRP hunowedzera kubva pa1 kusvika ku5 pane huwandu (husina kutsanangurwa). При концентрациях глюкозы >12,5% все концентрации гипертонической глюкозы снижали количество тромбоцитов, а MPV концентрации гипертонической глюкозы снижали количество тромбоцитов, а MPV повышам песни : тромбоцитов PRP увеличивалось от 1- до 5-кратных концентраций сравнению с исходными концентрациями цельной крови, в топи от видео, в концентрациями Pahuwandu hweglucose >12.5%, huwandu hweglucose hweBP hwakaderera huwandu hwemaplatelet uye huwandu hweMPV hwakakwira mumaplatelet ePRP: Huwandu hwemaplatelet ePRP hwakawedzera ka1 kusvika ka5 zvichienzaniswa nehuwandu hweropa rose rekutanga, zvichienderana nenzira (sezvakatsanangurwa).Mufananidzo 1 unoratidza kuti huwandu hwemaplatelet hwakaderera neinenge 75% mushure mekusanganiswa mumvura uye ne20-30% mushure memaminitsi gumi nemashanu ekusanganiswa nemazinga akasiyana eglucose zvichienzaniswa nePRP yekutanga uye kusanganiswa kwe1:1 kwakagadziriswa kuti kuve nehuwandu (1- k1 nekugadziriswa kwehuwandu). k -1 kubereka).1 kubereka).
Huwandu hwemasero ari mu dilution yega yega hunoratidzwa sechikamu chidiki chenhamba yekutanga isati ya dilution.
MPV yakaderera zvishoma panguva yekugadzirwa kwePRP, pasina kuchinja kwakawedzerwa muhuwandu hwekusanganiswa kusvika 12.5% ​​​​mumvura kana glucose (kusanganisira 25% PRP glucose mixes) uye yakawedzera neinopfuura 20% mushure mekusanganiswa mu 50% glucose solution (Mufananidzo .2). ). Kusiyana neizvi, maerythrocyte haana kuratidza shanduko huru muhuwandu pane chero kusanganiswa kunze kweH2O.
Avhareji yehuwandu hwemasero mu dilution yega yega inoratidzwa sechikamu chehuwandu hwepakutanga usati wa dilution.
Kudzikira kwakafanana asi kusingaratidzike zvakanyanya muhuwandu hwemaplatelet uye kuwedzera kweCVR kwakaonekwa muBC yakavhenekerwa ne50% glucose (kuumbwa ne25% glucose). Tafura 2 inoenzanisa nhamba dzemasero nehuwandu hwemasero muropa rose rakasanganiswa mu50% dextrose nedata rePRP rechikamu chekutanga rakasanganiswa mu50% dextrose. Shanduko muhuwandu hweRBC neRBC MCV dzakanga dzisiri pachena uye dzakanga dzisiri pfungwa dzedu.
SD = kutsauka kwakajairwa, MD = musiyano wepakati pakati pemapoka, SE = kutsauka kwakajairwa kwepakati, RBC = erythrocytes, PLT = maplatelet, PRP = plasma yakapfuma muplatelet, WB = ropa rese
Mushure mekuwedzera D50W kuWB, huwandu hwekurasikirwa kweplatelet hwakagadziriswa nekusanganiswa hwaive 7.7% (310±73 vs. 286±96) zvichienzaniswa ne17.8% yekuderedzwa kwePRP muD50W (664±348 vs. 544±277). MPV WB yakawedzera ne16.8% (kubva pa10.1 ± 0.5 kusvika pa11.8 ± 0.6), ukuwo MPV PRP yakawedzera ne26% (9.2 ± 0.8 vs. 11.6 ± 0.7). Kunyangwe musiyano wepakati mukuderedza kuwanda kwemaplatelet uye kuwedzera kweMPV kwaive kwakanyanya nePRP, shanduko mukudzikira kwehuwandu hwemaplatelet mukati meWB dzaive dzakakosha (310 ± 73 kusvika 286 ± 96 (-7.7%); p = .06) uye kuwedzera kweMPV kwaive kwakakosha (10.1 ± 0.5 kusvika 11.8 ± 0.6 (+16.8) p < .001). Kunyangwe musiyano wepakati mukuderedza kuwanda kwemaplatelet uye kuwedzera kweMPV kwaive kwakanyanya nePRP, shanduko mukudzikira kwehuwandu hwemaplatelet mukati meWB dzaive dzakakosha (310 ± 73 kusvika 286 ± 96 (-7.7%); p = .06) uye kuwedzera kweMPV kwaive kwakakosha (10.1 ± 0.5 kusvika 11.8 ± 0.6 (+16.8) p < .001).Kunyangwe musiyano wepakati mukuderedza kuwanda kwemaplatelet uye kuwedzera kweCVR kwaive kwakanyanya nePRP, shanduko mukudzikira kwehuwandu hwemaplatelet mukati meWB yaive yakakosha (310 ± 73 kusvika 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (от 10,1 ± 0,5 до 11,8 ± 0,6 (+16,8) p < 0,001). Kuwedzera kweMPV kwaive kwakakosha (kubva pa10.1 ± 0.5 kusvika pa11.8 ± 0.6 (+16.8) p < 0.001).尽管PRP 在血小板计数减少和MPV 增加方面的平均差异显着更大，但WB内血小板计数减少的变化几乎是显着的（310 ± 73 至286 ± 96 (-7.7%);p = .06）和MPV 的增劝 ± 96.到11.8 ± 0.6 (+16.8) p <.001).尽管 PRP 在 血小板 计数 和 增加 方面的 平均 差异 显着 大 , 但 但 内血小板 话 平安显着 的 （（310 ± 73 至 286 ± 96 (-7.7%) ; p = .06）和MPV 的增加是显着的（10.1 ± 0.5 ± 0.5 ± 0.5 ± 0.5 ± 11.8 ± 6 p. .001.Kuchinja kwekudzikira kwehuwandu hwemaplatelet mukati meWB kwaive kwakakura (kubva pa310 ± 73 kusvika 286 ± 96 (-7.7%); p = 0.06), kunyangwe PRP yaive nemusiyano mukuru wepakati mukuderera kwehuwandu hwemaplatelet uye kuwedzera kweMPV. uye kuwedzera kweMPV kwaive kwakakosha.(от 10,1 ± 0,5 до 11,8 ± 0,6 (+16,8) р < 0,001). (kubva pa10.1 ± 0.5 kusvika 11.8 ± 0.6 (+16.8) p < 0.001).
Kuwanda kweglucose kwekupedzisira kwe20% kwaidiwa kuti pave nekuchinja kukuru muMPV, asi kuchinja kweMPV kwainyanya kuoneka pakuwanda kwekupedzisira kwe25%. Kurasikirwa kwemaplatelet kwakadzikama mushure mekudzikira kwekutanga. Takaona kudzikira kukuru kwekutanga kweCVR, zvisinei, CVR yakadzoserwa nekukurumidza pahuwandu hweglucose hwekupedzisira hwe25%, hwakanga hwakakwira zvakanyanya kupfuura huwandu hweCVR hwakaonekwa pahuwandu hwekupedzisira hweglucose hwe20% ne15% (Mufananidzo 3 uye kuruboshwe rweTafura 3; mabhokisi ane mumvuri). inoratidza p-values ​​​​≤ alpha neBonferroni correction ye0.01). Kwakavawo nekudonha kukuru kwekutanga kwenhamba yePLT, kwakaonekwa muchikamu chekutanga che 0-15 s, uye kwakaramba kwakasimba (kubva masekondi gumi nemashanu kusvika maminetsi makumi matatu; kuruboshwe rwetafura 4).
Kuwedzerwa kwehuwandu hwakasiyana hweglucose muropa rose kwakaita kuti pave nekuderera kukuru kweMPV zvichiteverwa nekudzoreredzwa kwehuwandu hweglucose neanopfuura 20%. Ngano inoratidza huwandu hweglucose mushure mekuderedzwa. D15, D20 naD25 zvakaitwa mukuderedzwa kwe1:1. D21 naD41 zvakaitwa mukuderedzwa kwe1:5.
Tafura 4 inoratidza shanduko muhuwandu hwemaplatelet kana hwakaderedzwa muglucose yehypertonic. Takaona hukama hunoenderana nedosi pakati pekudonha nekukurumidza kwenhamba dzePLT pakuderedzwa kwe1:1 uye pakuderedzwa kwe1:5. Tichienzanisa kuderedzwa kwe1:1 seboka rimwe chete nekuderedzwa kwe1:5, boka re1:1 rakadzikira nekukurumidza muhuwandu hwemaplatelet hushoma pane boka re1:5 66±48,000 (23%) zvichienzaniswa ne99±69,000 (37%). , p = 0.014) muboka re1:5. Mushure mekudzikira kwekutanga panzvimbo yekutanga yekuyera, huwandu hwemaplatelet sechikamu cheglucose hwakagadzikana (Mufananidzo 4).
Kana ropa rose rikawedzerwa kuglucose muchikamu che1:1, huwandu hwemaplatelet hunodzikira neanenge 25%. Zvisinei, apo ropa rose rakawedzerwa pachikamu che1:5, kudzikira kwacho kwakawedzera zvikuru - anenge 50%.
41% glucose yakawedzera MPV nekukurumidza uye zvakanyanya kupfuura 25% kana 21%. Mhedzisiro yeMPV inoratidzwa muMufananidzo 3. Pamamwe ese ma dilutions, hapana kudzikira kwekutanga kweMPV kwakaonekwa mushure mekuwedzera 50% glucose. Pakushandisa 25% glucose (glucose concentration 20.8% pakudilution yekupedzisira), shanduko muMPV yakaenzana nekuchinja kwe20% glucose pakudilution 1:1 (Mufananidzo 3). Kunyangwe shanduko muMPV pakutanga dzaive dzakakura pa41% mixed concentration pane pa25%, musiyano muMPV uri pakati pe41% ne25% mushure memaminitsi gumi nematanhatu wakanga usisina kukosha (Tafura 3, kurudyi). Zvinonakidzawo kuti 25% glucose yakawedzera MPV zvinobudirira kupfuura 20.8%.
Chidzidzo ichi che in vitro chakasimbisa zvishoma fungidziro yedu. Zvakaratidza kuti partial platelet lysis inogona kuitika kuburikidza nekusanganiswa kwedextrose, kukurumidza kugadzika kwemaplatelet ku hypertonicity yakanyanya, uye kuwedzera kukuru kweMPV zvichienderana nehuwandu hwe >25% hwe hypertonic dextrose. Zvakaratidza kuti partial platelet lysis inogona kuitika kuburikidza nekusanganiswa kwedextrose, kukurumidza kugadzika kwemaplatelet ku hypertonicity yakanyanya, uye kuwedzera kukuru kweMPV zvichienderana nehuwandu hwe >25% hwe hypertonic dextrose. Он показал потенциальный частичный лизис тромбоцитов примесью декстрозы, быструю аккомодацию частичный лизис тромбоцитов примесью декстрозы, быструю аккомодацию частичный лизис тромбоцитов примесью декстрозы, быструю аккомодацию частичный лизис тромбоцитов примесью декстрозы. повышение MPV в ответ на гипертоническую концентрацию декстрозы > 25%. Zvakaratidza kuti panogona kunge paine mukana wekuti platelet iite lysis ine dextrose, kukurumidza kugadzikana kwemaplatelet kusvika pahuwandu hwakanyanya hwe hypertonicity, uye kuwedzera kukuru kweMPV mukupindura kune huwandu hwe hypertonic dextrose >25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端高渗，2浓度的高渗葡萄糖时MPV 显着上升.它 显示 出 通速 适应 极端25% 浓度 高渗 葡萄糖 时 时 mpv 显着。。。. Он показывает потенциальный частичный лизис тромбоцитов смесями с глюкозой, быструю адаптацию тромбоцитов к экстремальпитом значительное увеличение MPV в ответ на концентрацию гипертонической глюкозы > 25%. Inoratidza mukana wekuti platelet iparare ne glucose mixes, kukurumidza kuchinja maplatelet kuti asvike pa hypertonicity yakanyanya, uye kuwedzera kukuru kweMPV mukupindura hypertonic glucose >25%.Kuwedzera kwekutanga kwaive kwakanyanya pa41.6% yeglucose, asi kuwedzera kweMPV kwakasvika pa25% yeglucose anenge maminetsi makumi maviri mushure mekusangana nayo.
Kuwanda kwemaplatelet kunokanganiswa neglucose. Takaona kuti huwandu hwePLT hunoderera pakuderedzwa kweglucose kwese. Kudzikira kwakanyanya kwehuwandu hwemaplatelet mukudzikiswa kweH2O (0%) kwePRP series kunogona kunge kwakabatana ne osmotic lysis. Neimwe nzira, izvi zvinogona kunge zviri chinhu chinokonzerwa nekuungana kweplatelet, asi izvi zvinopesana nekushaikwa kwekuchinja kweMPV pakuderedzwa uku. Izvi zvinoreva kuti mamwe maplatelet anonyanya kunzwa hypoosmolarity.
Muhuwandu hwese hweglucose muchikamu che1:1, huwandu hwePLT hwakaderera ne20-30%, kunyangwe neD5W (hypotonic pa252 mOsm), izvo zvinogona kuratidza mhedzisiro chaiyo yeglucose isiri-osmotic, sezvo PLT neMPV zvakaramba zvisina kuchinja pakuwedzera katatu kwehuwandu hweglucose. kubva kuD5W kusvika kuD25W. Kutaura zvazviri, huwandu hwePLT hwaiwanzowedzera zvishoma nekuwedzera kweosmolarity.
Kudzikira kwePLT pakati pe1:1 ne1:5 dilutions zvinoreva kuti mhedzisiro yekunyungudika inoenderana nehuwandu hwekutanga nehwekupedzisira hweglucose. Kana zvaingoenderana nehuwandu hwekutanga chete, munhu anotarisira kuona mutsauko mukuderedzwa kwePLT pakati pehuwandu hwe1:1. Asi isu hatidaro. Kana mhedzisiro yelysis ichingoenderana nehuwandu hwekupedzisira hweglucose, saka hatitarisiri mutsauko mukuru pakati pekunyungudika kwe20% 1:1 nekunyungudika kwe20.8% 1:5. Asi takazviita.
Kana kurasikirwa kweplatelet kukaitika nekuda kwe platelet lysis, chikamu che lysate chinoumbwa, mushure mezvo ma cytokines nezvinhu zvinokura zvinoburitswa munzvimbo iri kunze kwemuviri. Zvidzidzo zvakati wandei zvakaratidza kuti platelet lysate inoshanda zvakafanana nePRP semhinduro yekupararira [11]. PRP pachayo yakaratidzwa kuva mhinduro inoshanda yekurapa kuwedzera kwemasero [12-14].
Maplatelet asingashande anotenderera muchimiro chediski yakasimbiswa nezvikamu zvakasiyana-siyana zvemukati. Panguva yekushanda, anotora chimiro chedenderedzwa kana cheamoeba, zvichikonzera kuwedzera kwevhoriyamu. Kuwedzera kwevhoriyamu kunoda kuwedzera kwenzvimbo yepamusoro, inova mhedzisiro yekubuda kwe open tubule system (OCS) uye kuwedzerwa kwe exocytic granules ku membrane. Zvinoramba zvichionekwa kana kuwedzera kweMPV kunokonzerwa ne hypertonic glucose kunosanganisira imwe kana ese ari maviri maitiro aya, asi kana iri yekupedzisira, kuwedzera kweMPV kunoratidza degranulation.
Chidzidzo ichi chakaratidza kuti kuwanda kweglucose muropa rose kana kuti maplatelet eropa kwakawedzera muMPV mukati memaminitsi gumi nemashanu nekuwedzera kweglucose muropa iri 25% ne41.6%, zvichiteerana.
Kuwedzera kweplatelet MPV kunogona kunge kuri nekuda kwekuwedzera kwe microtubule tangles dzakatenderedza nekuda kwe calcium increase. Liu et al. Glucose yakaratidzwa kuti inodzora calcium increase kuburikidza ne platelet TRPC6 channel [6]. Fungidziro yedu ndeyekuti glucose inokonzera kugadzikana kwe microtubule tangles, zvichikonzera kuwedzera kwe MPV uye platelet sensitization uye/kana activation. Zvisinei, tichitarisa zvatakawana, ichi chikamu chenyaya. Mumiedzo yedu, hapana concentration iri pasi pe D25W yakaguma nekuwedzera kwe MPV. Tichifunga kuti hatina kuyedza kusangana ne glucose concentrations pakati pe 12.5% ​​​​ne 25%, mhedzisiro yedu ye phase 1 inoratidza kuti panogona kunge paine threshold muhuwandu uhwu hwe glucose concentrations hunotungamira kukuwedzera kwe MPV. Kumwe kuyedza muzvikamu 3 ne 4 kwakaratidza kuti 20-25% glucose inoita kunge ndiyo threshold yeizvi, asi hazvisati zvajeka kuti nei.
Takaonawo kudzikira kweMPV kwe~9% mushure mekutenderera. Hazvisi pachena kana kudzikira uku kweMPV kuri kukonzerwa nemaplatelet akakura uye akawandisa akavharirwa muRBC layer yecentrifuge. Kucherechedzwa uku kungave kwakakosha kune vanachiremba sezvo zvingareva kuti maplatelet ePRP idiki uye isina kusimba zvakanyanya yemaplatelet eWB.
Muchidzidzo chakapfuura, takaratidza kuti kugadzirira PRP nenzira dzemaoko hakudhuri [8]. Kana glucose ikaita kuti maplatelet etishu kana PRP anzwe zviri nyore, kana kuti PRP ikagadzirwa nechikamu che lysate properties, izvi zvinogona kuwedzera kugadzurwa uye kuderedza kudiwa kwekurapwa. Nokudaro, kusanganiswa kwePRP neglucose yakawandisa kunogona kuva nemutengo wakaderera kupfuura PRP kana glucose chete.
Chidzidzo chedu chine zvikanganiso zvakawanda. Chekutanga, tinoshandisa PRP yakawanikwa kubva munzira dzakasiyana-siyana. Izvi zvinogona kukonzera mhedzisiro inopikisana. Chechipiri, hatina kukwanisa kuita ongororo ye biochemical yechero yemasampuli edu kuti tione kana platelet activation yakaitika. Tinoda kuyera P-selectin, platelet factor 4, monocytic platelet aggregates, kana zvimwe zviratidzo zve platelet activation kuti tinzwisise zviri nani dhigirii kana kuvapo kwe alpha granule degranulation, asi izvi zviri kunze kwechinangwa chechidzidzo ichi. Chechitatu, hatina kukwanisa kusimbisa ne electron microscopy kana dzimwe nzira kuti kuwedzera kweMPV muma glucose-exposed platelets kwakakonzerwa nemhedzisiro pama microtubule tangles.
Kusanganiswa kweWB kana PRP ne 25% glucose kwakawedzera MPV, zvichiratidza kutanga kwekushanda kweplatelet, kunyangwe chidzidzo ichi chisina kuratidza kufambira mberi kwekuungana kana kudonha kwegranulation. Musanganiswa weglucose we hypertonic wakaguma nekurasikirwa kweplatelet, zvichida zvichimiririra lytic effect. Kushanda zvishoma kana lysis yemaplatelet kunogona kukonzera kugadzikiswa kwetishu mushure mekubayiwa platelet. Hazvisi pachena kuti shanduko idzi dzingakonzera mhedzisiro ipi yekiriniki. Zvidzidzo zvakaenderera mberi zvakaratidza kuyera kwakarurama kwekushanda kana lysis uye zvakaongorora mhedzisiro dzakasiyana dzekiriniki dzemusanganiswa weglucose we hypertonic neWB kana PRP.
Kurapa nekuwedzera glucose inzira iri nyore uye isingadhuri yekurapa inovandudza masero iri kuwedzera nekukurumidza uye inotsigira tsvakurudzo yekiriniki. Chidzidzo ichi chinoratidza nzira yemuviri iyo, kana ikasimbiswa, inogona kutibatsira kunzwisisa chikamu chenzira yekuvandudza masero yekurapa nekuwedzera masero.
Biomedical and Health Informatics paYunivhesiti yeMissouri, Kansas City School of Medicine, Kansas City, USA
Vanhu Vanobatanidzwa: Vese vakapinda muchidzidzo ichi vakapa mvumo kana kuti havana. Sangano reInternational Society for Cellular Medicine rakapa mvumo yeICMS-2017-003. Nzira inotevera yatenderwa kuti ishandiswe zvakare neInstitutional Review Board yeInternational Society for Cellular Medicine: Musoro: Kuverengwa kwemuuyo wemishonga yeplasma yakapfuma muplatelet zvichibva pahuwandu hwemaplatelet eCBC. Vanhu Vanobatanidzwa Mumhuka: Vese vanyori vakasimbisa kuti hapana mhuka kana matishu akabatanidzwa muchidzidzo ichi. Kusawirirana Kwezvido: Zvichienderana neICMJE Uniform Disclosure Form, vanyori vese vanozivisa zvinotevera: Ruzivo rwekubhadhara/sevhisi: Vese vanyori vanozivisa kuti havana kuwana rutsigiro rwemari kubva kune chero sangano rebasa rakatumirwa. Hukama Hwemari: Vese vanyori vanozivisa kuti parizvino kana mukati memakore matatu apfuura havana hukama hwemari nechero sangano ringave richifarira basa rakatumirwa. Humwe Hukama: Vese vanyori vanozivisa kuti hapana humwe hukama kana mabasa anogona kukanganisa basa rakatumirwa.
Harrison TE, Bowler J, Reeves K et al. (Chivabvu 17, 2022) Mhedzisiro yeglucose pahuwandu hwemaplatelet uye vhoriyamu: mhedzisiro yemushonga unovandudza muviri. Cure 14(5): e25081. doi:10.7759/cureus.25081
© Kodzero dzeKodzero 2022 Harrison nevamwe. Ichi chinyorwa chakavhurika chakagoverwa pasi pemitemo yeCreative Commons Attribution License CC-BY 4.0. Kushandiswa, kugoverwa, uye kuburitswazve muchidimbu chero nzira kunobvumidzwa, chero bedzi munyori wekutanga nekwakabva vapihwa ruremekedzo.