"Enweghị obi abụọ na obere ìgwè ụmụ amaala nwere echiche na obi ike nwere ike ịgbanwe ụwa. N'eziokwu, ọ bụ naanị ya nọ ebe ahụ."
Ọrụ Cureus bụ ịgbanwe ụdị mbipụta ọgwụ ogologo oge, nke ntinye nyocha nwere ike ịdị oke ọnụ, dị mgbagwoju anya, ma were oge.
plasma/prp nke jupụtara na platelet, mmeghari anụ ahụ, ime ka platelet rụọ ọrụ, ọgwụgwọ mmụba glucose, platelet, ọgwụgwọ mmụba
Kpọọ akụkọ a dị ka: Harrison TE, Bowler J, Reeves K, et al. (Mee 17, 2022) Mmetụta nke glucose na ọnụọgụ platelet na olu: mmetụta maka ọgwụ regenerative. Ọgwụgwọ 14(5): e25081. doi:10.7759/cureus.25081
A na-ejikarị plasma bara ụba na platelet (PRP) na mmiri glucose hypertonic eme ihe maka ịgba ọgwụ n'ime ọgwụ ọhụrụ, mgbe ụfọdụ ọnụ. A kọbeghị na mbụ mmetụta nke hypertonic glucose na platelet lysis na activation. Anyị nwalere mmetụta nke mmụba nke glucose na platelet na erythrocyte ọnụọgụ, yana olu mkpụrụ ndụ na PRP na ọbara dum (WB). Mbelata ngwa ngwa na ọnụọgụ platelet mere na ngwakọta glucose niile agwakọtara na PRP ma ọ bụ ọbara dum, nke kwekọrọ na akụkụ nke lysis. Mgbe nkeji mbụ gachara, ọnụọgụ platelet nọgidere na-akwụsi ike, nke na-egosi na platelet fọdụrụnụ na-anabata ngwa ngwa ruo oke oke (> 2000 mOsm). Mgbe nkeji mbụ gachara, ọnụọgụ platelet nọgidere na-akwụsi ike, nke na-egosi na platelet fọdụrụnụ na-anabata ngwa ngwa ruo oke oke (> 2000 mOsm). После первой минуты количество тромбоцитов trombotsytov DO эkstremalnoho (>2000 mОsm) gypertonusa. Mgbe nkeji mbụ gachara, ọnụọgụ platelet ahụ nọgidere na-adịgide, nke na-egosi na platelet ndị fọdụrụ na-anabata ngwa ngwa ruo oke oke (> 2000 mOsm).第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端（> 2000 mOsm.2000 mOsm）高渗状态。 После первой минуты количество тромбоцитов trombotsytov ka эkstremalnomu (> 2000 mОsm) gyperosmolyarnomy sostoyt. Mgbe nkeji mbụ gachara, ọnụọgụ platelet ahụ nọgidere na-adịgide, nke na-egosi na platelet ndị fọdụrụ agbanweela ngwa ngwa ruo n'ọnọdụ oke osmolar (>2000 mOsm).Ọnụọgụ glucose nke 25% na karịa mere ka mmụba dị ukwuu na nkezi olu platelet (MPV), nke na-egosi mmalite nke mmalite nke ọrụ platelet. A chọrọ ọmụmụ ihe ndị ọzọ iji chọpụta ma platelet lysis ma ọ bụ ọrụ na-eme, nakwa ma ntụtụ glucose hypertonic naanị ma ọ bụ na njikọta ya na PRP nwere ike inye uru ahụike ọzọ.
N'afọ 1950, dọkịta na-awa ahụ nke Amerịka bụ́ George Hackett chọpụtara na ọ nwere ike iwepụ mgbu nkwonkwo na azụ n'ọtụtụ ndị ọrịa site n'ịgba ọgwụ mgbochi n'ime akwara na akwara. Nnwale ndị o mere na oke bekee gosiri na ọgwụgwọ ahụ, nke ọ kpọrọ ọgwụgwọ mgbochi, mere ka akwara ndị ahụ too ma sie ike. Ọmụmụ ihe gbasara histological egosila na a na-emepụta collagen ọhụrụ n'oge usoro a [1].
N'ime iri afọ ole na ole mbụ, a nwalere ọtụtụ ụzọ dị iche iche esi kesaa ihe. Ka ọ na-erule afọ 1990, ọtụtụ ndị ọkachamara lere nnukwu glucose anya dị ka ụzọ kachasị nchebe na nke kachasị dị irè. Agbanyeghị, usoro ọrụ ka edoghị anya.
Ọ bụ naanị ọmụmụ ihe ahụike ole na ole ka e mere na narị afọ nke 20 mgbe ọrụ Hackett gasịrị. Agbanyeghị, n'afọ 2000, e nwere mmasị ọhụrụ, e mechakwara ọtụtụ nnwale ahụike gara nke ọma nke ọgwụgwọ ịba ụba maka ọgwụgwọ mgbu azụ dị ala [2], osteoarthritis nke ikpere [3], na lateral epicondylitis [4].
Mmụgharị anụ ahụ chọrọ isonye na mkpụrụ ndụ stem. Ya mere, oke glucose ga-akpali n'ụzọ ụfọdụ ịkwaga, ịmụgharị, na iche iche nke mkpụrụ ndụ stem. Anyị na-eche na platelets nwere ike ịrụ ọrụ dị ka ndị ozi nakwa na oke glucose nwere ike ime ka platelets wepụta cytokines na ihe ndị na-eto eto, si otú a na-akwalite usoro mmeghari ohuru, karịsịa mbugharị mkpụrụ ndụ stem gaa na mpaghara nwere oke glucose.
Mmelite platelet na-ebute mmụba nke calcium dị n'ime sel [5]. Liu et al. na 2008 gosiri na ọkwa glucose dị elu na-eme ka ọrụ nke ọwa transient receptor potential canonical type 6 (TRPC6) dị na plasma membrane dịkwuo elu, nke na-eduga na mmụba nke calcium ion n'ime platelet [6]. Ọmụmụ ihe ọzọ gosiri na ikpughe nke mpaghara microtubule na calcium ion na-akpata izu ike, mmụba, na mgbanwe nke mpaghara oke, nke na-akpata mgbanwe n'ụdị site na diski gaa na gburugburu, na-ebute nkezi olu platelet (MPV) [7].
Echiche anyị n'ọmụmụ ihe a bụ na mkpughe nke platelet n'ebe glucose dị elu na-emetụta mpaghara microtubule na gburugburu sel, na-eduga na mmụba na MPV.
Ndị niile sonyere bịanyere aka na fọm nkwenye amaara ama mgbe akọwara nkọwa nke ọmụmụ ihe ahụ ma tupu ha anata ihe nlele ahụ. N'ọmụmụ ihe a, naanị ihe nlele PRP nwere hematocrit karịrị 2% ka ejiri mee ihe ka e wee nwee ike itinye ọnụọgụ erythrocyte (erythrocyte) na nkezi olu nke mkpụrụ ndụ ọbara uhie (MCV) maka ntụnyere.
E mere nnyocha a n'ime usoro anọ, nke mbụ bụ PRP na nke fọdụrụ bụ ọbara dum (Tebụl 1). Dịka akọwara na mbụ [8], a gbakọrọ ike centrifugal niile (RCF, g-force) site na etiti (Rmid, na cm) nke kọlụm ọbara dị na sirinji centrifugal. Anyị họọrọ iji MPV dị ka ihe nrịbama nke mmetụta platelet na ọnụọgụ platelet dị ka ihe ngosi nke lysis platelet nwere ike, nke a pụrụ ịtụ ha abụọ ngwa ngwa na ndị nyocha hematology ọkọlọtọ.
N'oge mbụ, ndị ọrụ afọ ofufo iri anọ na asaa nyere ihe nlele ọbara—otu tube nke ethylenediaminetetraacetic acid (EDTA) na otu ihe nlele ọbara PRP zuru oke (anticoagulated with sodium citrate (NaCl, 3%)) (Tebụl 1). Tinye ihe nlele ahụ n'ime tube ozugbo. Emere ọnụọgụ ọbara zuru oke (CBC) na ihe nlele EDTA n'ime atọ, e wee nyochaa ihe nlele NaCl n'ime atọ maka nyocha CBC, wee kwadebe PRP site na ụzọ dị iche iche akọwara n'elu [8]. E ji centrifugation kwadebe ihe nlele PRP niile na 900–1000 g. Gwakọta ihe nlele PRP ọ bụla na ihe ngwakọta vortex maka sekọnd 5–10, wee kewaa ihe nchịkọta ise nke 0.5 ml n'ime tubes.
Iji chọpụta mmetụta nke mkpughe platelet na mmụba nke glucose, a gwakọtara ọnụọgụgụ hà nhata (0.5 ml) nke 0%, 5%, 12.5%, 25%, na 50% glucose n'ime mmiri na ihe nlele platelet iji nweta 0%, 2.5% 6.25%, 12.5% ​​​​na 25% nke ngwakọta glucose ma gwakọta tubes ahụ na ihe nlele tube maka nkeji 15. A nyochachara TAC nke ngwakọta ọ bụla n'ụzọ atọ mgbe nkeji 15 gachara. A tụlere ọnụọgụgụ platelet (PLT), ọnụọgụ RBC, MCV, na MPV maka tube ọ bụla, a gbakọọkwa ọnụọgụ platelet, ọnụọgụ RBC, MCV, na MPV maka ihe nlele PRP niile.
Mgbe emechara usoro mbụ nke nchịkọta data, anyị hụrụ mmụba dị ukwuu na olu platelet na PRP platelet mgbe etinyere D50W. PRP platelet anaghị anọchite anya platelet niile dị n'ọbara, PRP medium dịkwa iche na WB medium. Ya mere, anyị kpebiri ime nnwale nkebi nke abụọ nke mmetụta nke itinye D50W na ọbara dum.
Maka agba nke abụọ, anyị họọrọ nha ihe nlele nke 30 dabere na nsonaazụ sitere na usoro mbụ, dịka akọwara na ngalaba nyocha. N'usoro a, ndị ọrụ afọ ofufo iri abụọ nyere ihe nlele ọbara (Tebụl 1). A dọbara ọbara dum (1.8 ml) n'ime sirinji 3 ml ma jiri 0.2 ml 40% NaCl mee ka ọ dị mma. A gwakọtara sirinji ọbara dum maka sekọnd ise na igwekota vortex wee nyochaa CBC na atọ. Mgbe nyochachara, etinyere ọbara anticoagulated na 2 ml nke 50% glucose na sirinji 5 ml (njupụta glucose ikpeazụ bụ ihe dị ka 25% (D25) ma tinye ya na tube shake maka nkeji 30. Mgbe nkeji 30 gachara, a nyochachara D25/CBC na sirinji WB na atọ. A tụlere ọnụọgụ platelet, ọnụọgụ RBC, MCV, na MPV kwa sirinji nkezi, a gbakọọkwa nkezi ọnụọgụ PLT, RBC, MCV, na MPV maka ihe nlele ọ bụla tupu na mgbe etinyere glucose.
Ebe ọ bụ na platelets dị n'ọbara dum na-apụtakarị na glucose hypertonic n'oge ọgwụgwọ glucose na-agbasa n'ihi obere ọgwụ mgbochi, ọ bụghịkwa ihe a na-ahụkarị ijikọta PRP na glucose hypertonic tupu a gbaa ya, anyị kpebiri ịmụ banyere glucose hypertonic na njikọ ya na WB na Nkebi nke 1. Nzọụkwụ nke Atọ na nke Anọ. N'ọkwa nke ọ bụla, ndị ọrụ afọ ofufo iri abụọ nyere 7-8 ml nke ACD-A (acid nwere trisodium citrate (22.0 g/l), citric acid (8.0 g/l) na glucose (24.5 g/l), solution dextrose citrate) maka ọgwụ mgbochi ọbara (Tebụl 1). Naanị ngwakọta glucose karịrị 12.5% ​​ka ejiri iji chọpụta pasentị njedebe nke metụtara mmụba na MPV. Na nke atọ, a na-etinye 1 ml nke ọbara n'ime tube nnwale. Mgbe ahụ gwakọta ọbara ahụ na igwekota vortex ruo sekọnd iri site na itinye 1 ml nke 30% glucose, 40% glucose, ma ọ bụ 50% glucose na tube ahụ iji nweta mkpokọta glucose ikpeazụ nke 15%, 20%, na 25%, n'otu n'otu. E nyochachara ihe nlele ọbara glucose maka CBC ozugbo agwakọtara ya ma mee ya ugboro ugboro kwa nkeji abụọ maka nkeji iri atọ.
N'oge ngwakọta mbụ, itinye 1:1 hypertonic glucose na WB ma ọ bụ PRP na-ekpughe platelets n'ime oke karịa 25% ruo ọtụtụ sekọnd. Na nzọụkwụ nke anọ, iji nyochaa mmetụta nke hypertonic glucose na obere oke mbụ ma nwalee oke elu nke mmetụta glucose, anyị tinyere naanị obere ọbara na D25W ma ọ bụ D50W. Tinye 1 ml nke D25W ma ọ bụ D50W n'ime tube ma tinye 0.2 ml nke WB ka ị na-agbagharị ihe nlele ahụ maka sekọnd 10. N'ọnọdụ ndị a, a hụrụ ọbara na glucose na oke ihe dị ka 20% karịa oke ikpeazụ, kama 50% karịa oke ikpeazụ dịka na Nkebi nke 3, nke butere oke glucose ikpeazụ nke 20.8% na 41.6%. A tụlere ihe nlele agwakọtara n'otu oge ahụ dịka na nzọụkwụ nke 3.
Na nzọụkwụ mbụ nke usoro mmịpụta glucose ọ bụla, e were ihe nlele 30 n'ihi na nke a bụ nha ihe nlele kwesịrị ekwesị maka ọmụmụ ihe nnwale [9]. Na njedebe nke usoro ọ bụla (gụnyere nke mbụ), nyochaa nha ihe nlele ahụ zuru oke site na iji usoro eji chọpụta nha ihe nlele achọrọ iji chọpụta nkezi nke mgbanwe nsonaazụ na-aga n'ihu na otu ọnụọgụ mmadụ. Usoro n = Z2 x SD2 /E2. Na nhazi a, Z bụ akara Z, SD bụ mgbanwe ọkọlọtọ, na E bụ njehie achọrọ [10]. Alfa anyị bụ 0.05, nke kwekọrọ na uru Z nke 1.96, anyị na-atụkwa anya njehie nke 5 (na pasent). Ya mere, anyị na-edozi maka n = (1.962 x SD2)/52. Nsonaazụ gosiri na nha ihe nlele achọrọ maka nke ọ bụla pere mpe karịa ọnụọgụgụ ahụ anakọtara n'ezie.
N'oge nke 1, 3 na 4, site n'iji ihe karịrị otu glucose, a tụlere mmetụta nke njupụta glucose dị iche iche site n'ịtụnyere mgbanwe nkebi dị n'etiti oge 0 na oge ọ bụla sochirinụ (nkebi nke 1 na nkeji 15, oge nke 3 na nkeji 15). na anọ na sekọnd 15, wee jiri nkeji abụọ ọ bụla.) A tụnyere ọnụego mgbanwe maka oge ọ bụla site na iji ule Mann-Whitney U n'ihi na data ahụ esoghị nkesa nkịtị dịka ule Shapiro-Wilk siri chọpụta. Ebe ọ bụ na emere nyocha 1-ruo-1 nke ọtụtụ otu (ise) na nzọụkwụ nke mbụ, nke atọ na nke anọ (ise na mkpokọta), e mere ndozi Bonferroni iji gbanwee uru alpha achọrọ ka ọ bụrụ ≤0.01 mana ọ bụghị ≤0.05.
Mbelata ọnụọgụ platelet site na mkpokọta niile nke dextrose hypertonic na mmụba na MPV na platelet PRP na njupụta dextrose >12.5%: Ọnụọgụ platelet PRP rịrị elu site na njupụta otu ruo ugboro ise ma e jiri ya tụnyere ọbara dum nke mbụ, na-agbanwe dịka usoro ahụ si dị (anaghị egosipụta ya). Mbelata ọnụọgụ platelet site na mkpokọta niile nke dextrose hypertonic na mmụba na MPV na platelet PRP na njupụta dextrose >12.5%: Ọnụọgụ platelet PRP rịrị elu site na njupụta otu ruo ugboro ise ma e jiri ya tụnyere ọbara dum, na-agbanwe dịka usoro ahụ si dị (anaghị egosipụta ya). Уменьшение Уменьшение количества тромбоцитов при всех концентрациях гипертонической декстрозы na увеличение MPV. концентрации декстрозы > 12,5%: количество тромбоцитов PRP увеличилось в 1-5 раз по сравнению зависимости от метода (bụghị ihe efu). Mbelata ọnụọgụ platelet na mkpokọta dextrose hypertonic niile yana mmụba MPV na platelet PRP na njupụta dextrose > 12.5%: ọnụọgụ platelet PRP mụbara ugboro 1-5 ma e jiri ya tụnyere ọbara dum, dabere na usoro (egosighi ya). ).在> 12.5% ​​的葡萄糖浓度下，所有浓度的高渗葡萄糖降低血小板计数，PRP 血小板中MPV增加：与基线全血相比， PRP 血小板计数从浓度的1 倍上升到5 N'ihe karịrị 12.5% ​​nke glucose, oke glucose na-ebelata ọnụọgụ ọbara, MPV PRP na-abawanye n'ọbara: ma e jiri ya tụnyere GP, ọnụọgụ ọbara PRP na-abawanye site na 1 ruo 5 nke mkpokọta ahụ (akọwaghị ya). При концентрациях глюкозы >12,5% все концентрации в тромбоцитах PRP: количество тромбоцитов PRP увеличивалось от 1- до 5-кратных концентраций по сравнению концентрациями цельной крови, в зависимости от метода (nе описано ). N'oge njupụta glucose karịrị 12.5%, njupụta glucose ọbara mgbali elu niile belatara ọnụọgụ platelet ma mụbaa MPV na PRP platelet: ọnụọgụ platelet PRP mụbara site na 1 ruo 5 ma e jiri ya tụnyere njupụta ọbara dum, dabere na usoro ahụ (dịka akọwara).Foto nke 1 na-egosi na ọnụọgụ platelet belatara ihe fọrọ nke nta ka ọ bụrụ 75% mgbe a gwakọtara ya na mmiri na site na 20-30% mgbe nkeji iri na ise nke ịgbaze ya na oke glucose dị iche iche ma e jiri ya tụnyere PRP nke mbụ na mgbaze 1:1 nke agbanwere maka olu (1- k1 na mgbazi olu). ọmụmụ k- 1). 1 ọmụmụ).
A na-egosi ọnụọgụgụ mkpụrụ ndụ dị na ngwakọta ọ bụla dị ka obere akụkụ nke ọnụọgụ mbụ tupu ngwakọta ahụ.
MPV belatara ntakịrị n'oge mmepụta PRP, na-enweghị mgbanwe ọzọ na mkpokọta mmiri ara ehi ruo 12.5% ​​​​na mmiri ma ọ bụ glucose (gụnyere 25% ngwakọta glucose PRP) ma mụbaa site na ihe karịrị 20% mgbe agbazechara ya na mmiri ara ehi 50% glucose (Foto .2). N'ụzọ dị iche, erythrocytes egosighi mgbanwe dị ukwuu na olu na mgbaze ọ bụla ma e wezụga H2O.
A na-egosipụta nkezi olu nke mkpụrụ ndụ dị na ngwakọta ọ bụla dị ka pasentị nke olu mbụ tupu ngwakọta ahụ.
E hụrụ mbelata yiri nke ahụ mana ọ dịghị oke njọ na ọnụọgụ platelet na mmụba na CVR na BC nke e kpughere na 50% glucose (iji mepụta ya na 25% glucose). Tebụl nke abụọ na-atụnyere ọnụọgụ sel na olu sel n'ọbara dum nke a gwakọtara na 50% dextrose yana data PRP nke 1 nke a gwakọtara na 50% dextrose. Mgbanwe na ọnụọgụ RBC na RBC MCV abụghị ihe doro anya ma ọ bụghị ihe anyị lekwasịrị anya.
SD = mgbanwe ọkọlọtọ, MD = ọdịiche nkezi dị n'etiti otu, SE = mgbanwe ọkọlọtọ nke ọdịiche nkezi, RBC = erythrocytes, PLT = platelets, PRP = plasma bara ụba na platelets, WB = ọbara dum
Mgbe etinyere D50W na WB, pasentị nke mfu platelet edoziri na dilution bụ 7.7% (310±73 vs. 286±96) ma e jiri ya tụnyere 17.8% maka dilution PRP na D50W (664±348 vs. 544±277). MPV WB mụbara site na 16.8% (site na 10.1 ± 0.5 ruo 11.8 ± 0.6), ebe MPV PRP mụbara site na 26% (9.2 ± 0.8 vs. 11.6 ± 0.7). Ọ bụ ezie na ọdịiche nkezi dị na mbelata ọnụọgụ platelet na mmụba MPV dị oke ukwuu na PRP, mgbanwe na mbelata ọnụọgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị mkpa (310 ± 73 ruo 286 ± 96 (-7.7%); p = .06) na mmụba na MPV dị oke mkpa (10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p < .001). Ọ bụ ezie na ọdịiche nkezi dị na mbelata ọnụọgụ platelet na mmụba MPV dị oke ukwuu na PRP, mgbanwe na mbelata ọnụọgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị mkpa (310 ± 73 ruo 286 ± 96 (-7.7%); p = .06) na mmụba na MPV dị oke mkpa (10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p < .001).Ọ bụ ezie na ọdịiche nkezi dị na mbelata ọnụọgụ platelet na mmụba CVR dị ukwuu nke ukwuu na PRP, mgbanwe na mbelata ọnụọgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị mkpa (310 ± 73 ruo 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (от 10,1 ± 0,5 na 11,8 ± 0,6 (+16,8) p <0,001). mmụba dị na MPV dị oke mkpa (site na 10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p < 0.001).尽管PRP 在血小板计数减少和MPV 增加方面的平均差异显着更大，但WB内血小板计数减少的变化几乎是显着的（310 ± 73 至286 ± 96 (-7.7%)；p = .06）和MPV的增加是显着的（10.1 ± 0.5 到11.8 ± 0.6 (+16.8) p <.001）。尽管 PRP 在 血小板 计数 和 和 增加 方面 的 平均 差异 显着 大几乎 是 显着 的（（（310 ± 73 至 286 ± 96 (-7.7%) 0.6 (+16.8) p <.001).Mgbanwe dị na mbelata ọnụọgụ platelet n'ime WB fọrọ nke nta ka ọ bụrụ ihe dị mkpa (site na 310 ± 73 ruo 286 ± 96 (-7.7%); p = 0.06), ọ bụ ezie na PRP nwere nnukwu ọdịiche nkezi na mbelata ọnụọgụ platelet na mmụba MPV. mmụba na MPV dịkwa oke mkpa.(nke 10,1 ± 0,5 na 11,8 ± 0,6 (+16,8) р <0,001). (site na 10.1 ± 0.5 ruo 11.8 ± 0.6 (+16.8) p < 0.001).
A chọrọ ka ọnụọgụ glucose 20% ikpeazụ dị mkpa iji hụ mgbanwe dị ukwuu na MPV, mana mgbanwe dị na MPV pụtara ìhè karịa na mkpokọta ikpeazụ nke 25%. Mfu platelet kwụsiri ike mgbe mbelata mbụ gasịrị. Anyị hụrụ mbelata dị nkọ na CVR mbụ, agbanyeghị, eweghachiri CVR ngwa ngwa na mkpokọta glucose ikpeazụ nke 25%, nke dị elu karịa ọkwa CVR a hụrụ na mkpokọta glucose ikpeazụ nke 20% na 15% (Foto 3 na n'aka ekpe nke Tebụl 3; igbe ndị a gbanyere agbawa). na-egosi uru p ≤ alpha yana mmezi Bonferroni nke 0.01). Enwekwara mbelata dị nkọ na ọnụọgụ PLT mbụ, a hụrụ na usoro mbụ nke 0-15 s, wee nọgide na-eguzosi ike (site na nkeji 15 ruo nkeji 30; aka ekpe nke tebụl 4).
Mgbakwunye nke ọtụtụ glucose n'ọbara dum mere ka MPV belata ngwa ngwa ozugbo, wee nwetaghachi ihe karịrị 20%. Akụkọ ifo ahụ na-egosi na glucose dị n'ọbara mgbe a gbasasịrị ya. E mere D15, D20 na D25 na ngwakọta 1:1. E mere D21 na D41 na ngwakọta 1:5.
Tebụl nke 4 na-egosi mgbanwe na ọnụọgụ platelet mgbe a gwakọtara ya na hypertonic glucose. Anyị hụrụ mmekọrịta dabere na dose n'etiti mbelata ozugbo na ọnụọgụ PLT na mgbaze 1:1 na mgbaze 1:5. Site n'iji mgbaze 1:1 tụnyere mgbaze 1:5 dị ka otu otu na mgbaze 1:5, otu 1:1 nwere mbelata ozugbo na ọnụọgụ platelet na-erughị otu 1:5 66±48,000 (23%) megide 99±69,000 (37%). , p = 0.014) na otu 1:5. Mgbe mbelata mbụ gasịrị na ebe nha mbụ, ọnụọgụ platelet dị ka pasent nke glucose kwụsiri ike (Foto 4).
Mgbe etinyere ọbara dum na glucose na nha 1:1, ọnụọgụ platelet na-ebelata ihe dị ka 25%. Agbanyeghị, mgbe etinyere ọbara dum na nha 1:5, mbelata ahụ dị ukwuu - ihe dị ka 50%.
41% glucose mụbara MPV ngwa ngwa ma karịa 25% ma ọ bụ 21%. E gosiri nsonaazụ MPV na Foto nke 3. Na ngwakọta ndị ọzọ niile, a hụghị mbelata mbụ na MPV mgbe etinyere 50% glucose. Mgbe ejiri 25% glucose (njupụta glucose 20.8% na ngwakọta ikpeazụ), mgbanwe na MPV yiri mgbanwe na 20% glucose na ngwakọta 1:1 (Foto nke 3). Ọ bụ ezie na mgbanwe na MPV dị ukwuu na mbụ na mkpokọta 41% karịa na 25%, ọdịiche dị na MPV dị n'etiti 41% na 25% mgbe nkeji 16 gasịrị abụghịzi ihe dị mkpa (Tebụl nke 3, aka nri). Ọ dịkwa mma na 25% glucose mụbara MPV nke ọma karịa 20.8%.
Ọmụmụ ihe a na vitro kwadoro echiche anyị n'ụzọ ụfọdụ. O gosiri na dextrose na-eme ka platelet lysis dị n'ime ọbara dịkwuo mma, na-eme ka platelet dị ngwa ngwa ruo n'ókè dị oke njọ, nakwa mmụba dị ukwuu na MPV n'ihi oke dextrose dị n'ọbara karịrị pasentị iri abụọ na ise. O gosiri na dextrose na-eme ka platelet lysis dị n'ime ọbara dịkwuo mma, na-eme ka platelet dị ngwa ngwa ruo n'ókè dị oke njọ, nakwa mmụba dị ukwuu na MPV n'ihi oke dextrose dị n'ọbara karịrị pasentị iri abụọ na ise. Он показал потенциальный частичный . O gosiri na enwere ike inwe lysis nke platelet n'ime akụkụ ya na dextrose, nnabata platelet ngwa ngwa ruo oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi maka ọkwa dextrose hypertonic > 25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端，血小板快速适应极端，响应>不帗>2浓度的高渗葡萄糖时MPV 显着上升。它 显示 出 通过 葡萄糖 潜在 的 部分 一小板 溶解响应> 25% 浓度 高渗 葡萄糖 时 时 mpv 显着….. Он показывает потенциальный частичный . Ọ na-egosi ike nke nchịkọta platelet nke akụkụ site na ngwakọta glucose, mgbanwe ngwa ngwa nke platelet na oke hypertonicity, yana mmụba dị ukwuu na MPV na nzaghachi maka hypertonic glucose > 25%.Mmụba mbụ ahụ kacha elu na mkpughe glucose 41.6%, mana mmụba na MPV ruru 25% mkpughe glucose ihe dị ka nkeji iri abụọ mgbe mkpughe ahụ gasịrị.
Ọbara glucose na-emetụta mkpokọta platelet. Anyị hụrụ na ọnụọgụ PLT belatara na ngwakọta glucose niile. Mbelata dị ukwuu na ọnụọgụ platelet na ngwakọta H2O (0%) nke usoro PRP nwere ike ijikọ ya na osmotic lysis. N'aka nke ọzọ, nke a nwere ike ịbụ ihe e ji mee ihe site na njikọta platelet, mana nke a dị iche na enweghị mgbanwe MPV na ngwakọta a. Nchọpụta a pụtara na ụfọdụ platelet nwere mmetụta dị ukwuu na hypoosmolarity.
N'ime ngwakọta glucose 1: 1 niile, ọnụọgụ PLT belatara site na 20-30%, ọbụlagodi site na D5W (hypotonic na 252 mOsm), nke nwere ike igosi mmetụta kpọmkwem nke glucose na-abụghị osmotic, ebe ọ bụ na ma PLT na MPV anọgideghị na-agbanwe agbanwe na mmụba okpukpu atọ nke glucose. site na D5W ruo D25W. N'ezie, mkpokọta PLT na-abawanye ntakịrị ka osmolarity na-abawanye.
Mbelata nke PLT n'etiti 1:1 na 1:5 nke ngwakọta pụtara na mmetụta nke mgbaze dabere na ntinye glucose mbụ na nke ikpeazụ. Ọ bụrụ na ọ dabere naanị na ntinye glucose mbụ, mgbe ahụ mmadụ ga-atụ anya ịhụ ọdịiche dị na mbelata PLT n'etiti ntinye 1:1. Mana anyị anaghị eme ya. Ọ bụrụ na mmetụta nke mgbaze dabere naanị na ntinye glucose ikpeazụ, mgbe ahụ anyị anaghị atụ anya nnukwu ọdịiche dị n'etiti ntinye glucose 20% 1:1 na ntinye 20.8% 1:5. Ma anyị mere ya.
Ọ bụrụ na mfu platelet emee n'ihi lysis nke platelet, a na-emepụta lysate nke nwere akụkụ, mgbe nke ahụ gasịrị, a na-ewepụta cytokines na ihe ndị na-eme ka ọ too n'ime gburugburu ebe obibi. Ọtụtụ nnyocha egosila na lysate platelet fọrọ nke nta ka ọ dị irè dịka PRP dị ka ngwọta mmụba [11]. A gosila na PRP n'onwe ya bụ ngwọta dị irè maka ọgwụgwọ mmụba [12-14].
Platelet ndị na-adịghị arụ ọrụ na-agagharị n'ụdị diski e ji ọtụtụ ihe dị n'ime mee ka ọ sie ike. N'oge ọrụ ahụ, ha na-agbanwe ọdịdị gburugburu ma ọ bụ amoeba, nke na-ebute mmụba na olu. Mmụba na olu chọrọ mmụba na mpaghara elu, nke bụ nsonaazụ nke mmịpụta nke sistemụ tubule mepere emepe (OCS) na mgbakwunye nke granules exocytic na akpụkpọ ahụ. A ka ga-ekpebi ma mmụba na MPV nke glucose hypertonic kpatara gụnyere otu ma ọ bụ usoro abụọ a, mana ọ bụrụ na nke ikpeazụ, mgbe ahụ mmụba na MPV ga-egosi mmebi.
Nnyocha a gosiri na mkpughe nke nnukwu glucose na PRP ma ọ bụ platelet ọbara dum mere ka MPV mụbaa n'ime nkeji iri na ise yana mkpokọta glucose nke 25% na 41.6%, n'otu n'otu.
Mmụba na platelet MPV nwere ike ịbụ n'ihi mgbasa nke microtubule na-agbakọta gburugburu na nzaghachi maka mmụba calcium. Liu et al. Egosipụtala na glucose na-eme ka calcium na-agbakọta site na ọwa platelet TRPC6 [6]. Echiche anyị bụ na glucose na-eme ka microtubule na-agbakọta nwayọ, na-eduga na mmụba na MPV na mmetụta platelet na/ma ọ bụ mkpali. Agbanyeghị, site na nsonaazụ anyị, nke a bụ naanị akụkụ nke akụkọ ahụ. Na ule anyị, enweghị ntinye n'okpuru D25W butere mmụba na MPV. Ebe ọ bụ na anyị anwalebeghị mkpughe na ntinye glucose n'etiti 12.5% ​​​​na 25%, nsonaazụ nke usoro nke 1 anyị na-egosi na enwere ike ịnwe oke na oke glucose a nke na-eduga na mmụba na MPV. Nnwale ndị ọzọ na ọkwa nke 3 na 4 gosiri na glucose 20-25% yiri ka ọ bụ oke maka nke a, mana ọ ka edoghị anya ihe kpatara ya.
Anyị hụkwara mbelata nke ~9% na MPV mgbe centrifugation gasịrị. O doro anya ma mbelata a na MPV bụ n'ihi nnukwu platelets ndị dị arọ ma sie ike nke tọrọ n'ime oyi akwa RBC nke centrifuge. Nchọpụta a nwere ike ịdị mkpa nye ndị dọkịta n'ihi na ọ nwere ike ịpụta na platelets PRP bụ obere na obere oke nke platelets WB.
N'ọmụmụ ihe gara aga, anyị gosiri na nkwadebe PRP site na iji aka eme ihe adịghị ọnụ ala [8]. Ọ bụrụ na glucose na-eme ka platelets anụ ahụ ma ọ bụ PRP dịkwuo mfe ịrụ ọrụ, ma ọ bụ ọ bụrụ na emepụta PRP nwere akụkụ lysate, nke a nwere ike ime ka mmụba dịkwuo elu ma belata mkpa maka ọgwụgwọ. Ya mere, njikọta nke PRP na glucose a chịkọtara nke ukwuu nwere ike ịdị ọnụ ala karịa PRP ma ọ bụ glucose naanị.
Ọmụmụ ihe anyị nwere ọtụtụ adịghị ike. Nke mbụ, anyị na-eji PRP nke e si n'ọtụtụ ụzọ dị iche iche nweta. Nke a nwere ike ibute nsonaazụ na-emegiderịta onwe ha. Nke abụọ, anyị enweghị ike ime nyocha biochemical nke ihe nlele anyị ọ bụla iji chọpụta nke ọma ma ọ bụrụ na mwepụta platelet emeela. Anyị ga-achọ ịtụ P-selectin, platelet factor 4, monocytic platelet aggregates, ma ọ bụ ihe nrịbama ndị ọzọ nke mmegharị platelet iji ghọta nke ọma ogo ma ọ bụ ọnụnọ nke mmebi granule alpha, mana nke a karịrị oke ọmụmụ a. Nke atọ, anyị enweghị ike iji electron microscopy ma ọ bụ ụzọ ndị ọzọ gosi na mmụba nke MPV na platelets ndị nwere glucose bụ n'ihi mmetụta na microtubule tangles.
Ngwakọta nke WB ma ọ bụ PRP nwere 25% glucose mụbara MPV, nke na-egosi mmalite nke ọrụ platelet, ọ bụ ezie na ọmụmụ ihe a egosighi ọganihu nke nchịkọta ma ọ bụ mmebi. Ngwakọta glucose hypertonic butere mfu platelet, ikekwe na-anọchite anya mmetụta lytic. Mmelite akụkụ ma ọ bụ lysis nke platelet nwere ike ime ka anụ ahụ dịghachi ọhụrụ mgbe a gbasịrị platelet. O doro anya ihe nsonaazụ ahụike mgbanwe ndị a nwere ike ibute. Ọmụmụ ihe ndị ọzọ egosila nha ziri ezi nke mkpali ma ọ bụ lysis ma nyochaa mmetụta dị iche iche nke ngwakọta glucose hypertonic na WB ma ọ bụ PRP.
Ọgwụgwọ mmụba glucose bụ ọgwụgwọ mgbanwe dị mfe ma dị ọnụ ala nke na-agbasa ngwa ngwa ma na-akwado nchọpụta ahụike. Ọmụmụ ihe a na-egosi usoro physiological nke, ọ bụrụ na akwado ya, nwere ike inyere anyị aka ịghọta akụkụ nke usoro mgbanwe nke ọgwụgwọ mmụba.
Biomedical and Health Informatics na Mahadum Missouri, Kansas City School of Medicine, Kansas City, USA
Ndị Mmadụ: Ndị niile sonyere na nnyocha a nyere ma ọ bụ ekweghị. International Society for Cellular Medicine enyela nkwenye ICMS-2017-003. A kwadoro usoro a maka iji ya ọzọ site n'aka Institutional Review Board nke International Society for Cellular Medicine: Aha: Ngụkọta nke mkpụrụ ọgwụ plasma bara ụba na platelet dabere na ọnụọgụ platelet CBC nke mbụ. Ndị Anụmanụ: Ndị edemede niile kwadoro na ọ dịghị anụmanụ ma ọ bụ anụ ahụ metụtara na ọmụmụ ihe a. Esemokwu Mmasị: Dịka Fọm ICMJE Uniform Disclosure Form si dị, ndị edemede niile kwupụtara ihe ndị a: Ozi ịkwụ ụgwọ/ọrụ: Ndị edemede niile na-ekwupụta na ha enwetaghị nkwado ego site n'aka òtù ọ bụla maka ọrụ enyere. Mmekọrịta Ego: Ndị edemede niile na-ekwupụta na ha enweghị ugbu a ma ọ bụ n'ime afọ atọ gara aga mmekọrịta ego na nzukọ ọ bụla nwere ike inwe mmasị na ọrụ enyere. Mmekọrịta Ndị Ọzọ: Ndị edemede niile na-ekwupụta na ọ dịghị mmekọrịta ma ọ bụ ihe omume ndị ọzọ nwere ike imetụta ọrụ enyere.
Harrison TE, Bowler J, Reeves K et al. (Mee 17, 2022) Mmetụta nke glucose na ọnụọgụ platelet na olu: ihe ọ pụtara maka ọgwụgwọ regenerative. Ọgwụgwọ 14(5): e25081. doi:10.7759/cureus.25081
© Nwebiisinka 2022 Harrison na ndị otu ya. Nke a bụ akụkọ mepere emepe nke ekesara n'okpuru usoro nke Creative Commons Attribution License CC-BY 4.0. A na-ekwe ka ojiji, nkesa, na mmeputakwa na-enweghị njedebe n'ime ihe ọ bụla, ma ọ bụrụhaala na e nyere onye dere ya na ebe o si bịa otuto.