"Kaua rawa e ruarua ka taea e tētahi rōpū iti o ngā tāngata whai whakaaro nui, whai ngākau pono te whakarerekē i te ao. Koia anake te mea kei reira."
Ko te kaupapa matua a Cureus he whakarerekē i te tauira tawhito o te whakaputa tuhinga hauora, arā, he nui te utu, he uaua, he roa hoki te tuku rangahau.
Plasma/prp whai rawa i te pereti toto, whakahoutanga kiko, whakahohenga pereti toto, whakaora whakaputa huka toto, pereti toto, whakaora whakaputa toto
Whakahuatia tēnei tuhinga penei: Harrison TE, Bowler J, Reeves K, et al. (Mei 17, 2022) Te pānga o te huka ki te tatau me te rahinga o ngā pereti toto: ngā pānga ki te rongoā whakaora. Cure 14(5): e25081. doi:10.7759/cureus.25081
Ko te plasma whai-pereti (PRP) me ngā otinga huka toto toto tiketike e whakamahia whānuitia ana mō te werohanga i roto i te rongoā whakaora, i ētahi wā ka whakamahia ngātahi. Kāore anō kia pūrongohia i mua te pānga o te huka toto tiketike ki te pakaru me te whakahohenga o ngā pereti. I whakamatauria e mātou te pānga o te kukū huka toto teitei ki te maha o ngā pereti me ngā toto whero, tae atu ki te rahinga pūtau i roto i te PRP me te toto katoa (WB). I puta he whakahekenga tere ā-wāhanga o te maha o ngā pereti me te whakaranu i ngā ranunga huka toto katoa ki te PRP, ki te toto katoa rānei, e rite ana ki te pakaru ā-wāhanga. I muri i te meneti tuatahi, i noho pūmau te maha o ngā pereti toto, e tohu ana i te tere o te whakauru o ngā pereti toto toenga ki te tino hypertonicity (>2000 mOsm). I muri i te meneti tuatahi, i noho pūmau te maha o ngā pereti toto, e tohu ana i te tere o te whakauru o ngā pereti toto toenga ki te tino hypertonicity (>2000 mOsm). После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструю акктомодациромстостость экстремального (>2000 mОсм) гипертонуса. I muri i te meneti tuatahi, i noho pūmau te maha o ngā pereti toto, e tohu ana i te tere o te whakauru o ngā pereti toto toenga ki te tino hypertonicity (>2000 mOsm).第一分钟后，血小板计数保持稳定，表明残余血小板迅速适应极端（> 2000 mOsm。2000 mOsm）高渗状态. После первой минуты количество тромбоцитов оставалось стабильным, что указывает на быструю адаптацитов остатокви остатом экстремальному (>2000 mОсм) гиперосмолярному состоянию. I muri i te meneti tuatahi, i noho pūmau te maha o ngā pereti toto, e tohu ana i te tere o te urutau o ngā pereti toto e toe ana ki te āhua hyperosmolar tino nui (>2000 mOsm).Nā te nui o te huka toto i piki ake i te rahinga toharite o te pereti toto (MPV) i te 25% neke atu rānei, e tohu ana i te wā tōmua o te whakahohenga o te pereti toto. Me whai atu anō ngā rangahau hei whakatau mēnā ka puta te pakaru, te whakahohenga rānei o te pereti toto, ā, mēnā ka taea e te werohanga huka toto hypertonic anake, te whakauru rānei ki te PRP te whakarato i ētahi atu painga haumanu.
I ngā tau 1950, i kitea e te taote pokanga Amerikana a George Hackett ka taea e ia te whakaora tuturu i te mamae o ngā hononga me te tuara i roto i te tini o ngā tūroro mā te werohanga i tētahi otinga whakatipu ki roto i ngā uaua me ngā hononga. I whakaatuhia e āna whakamātautau ki ngā rāpeti ko te maimoatanga, i huaina e ia ko te whakaora whakatipu, i meinga ai ngā uaua kia nui ake, kia kaha ake. Kua whakaūhia e ngā rangahau histological ka puta he collagen hou i roto i tēnei tukanga [1].
I ngā tekau tau tuatahi, he maha ngā momo otinga tohatoha i whakamātauria. Tae rawa ki te tekau tau atu i 1990, i whakaaro te nuinga o ngā tohunga ko te kukū tiketike o te huka te tikanga haumaru me te tino whai hua. Heoi, kāore anō kia mārama te tikanga mahi.
He iti noa ngā rangahau haumanu i whakahaerehia i te rautau 20 whai muri i te mahi a Hackett. Heoi, i ngā tau 2000 i puta anō te hiahia, ā, he maha ngā whakamātautau haumanu angitu o te rongoā whakatipu i oti mō te rongoā i te mamae o te tuara o raro [2], te osteoarthritis o te turi [3], me te epicondylitis taha [4].
Ko te whakahoutanga o ngā kiko me whai wāhi mai ngā pūtau take. Nō reira, ko te nui o te hukahuka me ārahi i te hekenga, te whakaputa uri, me te wehewehenga o ngā pūtau take. E whakapae ana mātou ka mahi pea ngā pereti toto hei karere, ā, ko te nui o te hukahuka ka ārahi i ngā pereti toto ki te tuku i ngā cytokine me ngā mea whakatipu, ā, ka whakatairanga i ngā tukanga whakaora, inā koa te hekenga o ngā pūtau take ki ngā wāhi he nui te hukahuka.
Ko te whakahohenga o ngā pereti i ngā wā katoa ka puta i mua i te pikinga o te konupūmā ā-roto [5]. I whakaatu a Liu et al. i te tau 2008, ka whakanui ake ngā taumata huka toto tiketike i te mahi a ngā hongere momo TRPC6 (TRPC6) o te pūmanawa kaiwhiwhi whakawhiti i roto i te kiriuhi toto, ka hua ake te rerenga mai o ngā katote konupūmā ki roto i ngā pereti [6]. I whakaatu tētahi atu rangahau ko te whakaaturanga o te rohe taha microtubule ki ngā katote konupūmā ka puta te whakangā, te whānui, me te whakarerekētanga o te rohe taha, ka puta ai te huringa o te āhua mai i te kōpae ki te porowhita, ka hua ake te rahinga pereti toharite (MPV) [7].
Ko tā mātou whakapae i roto i tēnei rangahau, ko te pānga o te nui o te huka ki ngā pereti toto ka pā ki te rohe taha o te microtubule me te taiao ā-roto, ka piki ake te MPV.
I hainatia e ngā kaiuru katoa tētahi puka whakaae whai muri i te whakamāramatanga o ngā taipitopito o te rangahau, ā, i mua i te whiwhinga o ngā tauira. I roto i tēnei rangahau, ko ngā tauira PRP anake me te hematocrit neke atu i te 2% i whakamahia kia taea ai te whakauru i te maha o ngā toto whero (erythrocyte) me te rahinga toharite o ngā toto whero (MCV) hei whakataurite.
E whā ngā wāhanga o te rangahau i whakahaerehia, ko te wāhanga tuatahi ko te PRP, ā, ko ngā wāhanga e toe ana ko te toto katoa (Ripanga 1). E ai ki te whakaahuatanga o mua [8], i tatauhia ngā kaha porotītaha katoa (RCF, kaha-g) mai i te pūwāhi waenga (Rmid, i roto i te cm) o te pou toto i roto i te pūwero porotītaha. I whiriwhiria e mātou te whakamahi i te MPV hei tohu mō te whakanui i te pēreti toto me te tatau pēreti toto hei tohu mō te pakaru pea o te pēreti toto, ā, ka taea te ine ngāwari i ēnei mea e rua i runga i ngā tātaritanga hematology paerewa.
I te wāhanga tuatahi, e 47 ngā tūao i tuku tauira toto—kotahi te ngongo o te waikawa ethylenediaminetetraacetic (EDTA) me tētahi tauira toto katoa PRP (i whakakikoruatia ki te konutai citrate (NaCl, 3%)) (Ripanga 1). Whakanohoia tonutia te rōra ki roto i te ngongo. I mahia te tatau toto katoa (CBC) ki ngā tauira EDTA i roto i ngā tāruarua e toru, ā, i tātarihia ngā tauira NaCl i roto i ngā tāruarua e toru mō te tātari CBC, kātahi ka whakaritea te PRP mā ngā tikanga rerekē kua whakaahuatia i runga ake nei [8]. I whakaritea ngā tauira PRP katoa mā te centrifugation i te 900–1000 g. Whakaranua ia tauira PRP ki runga i te mīhini whakaranu vortex mō te 5–10 hēkona, kātahi ka wehea kia rima ngā aliquots 0.5 ml ki roto i ngā ngongo.
Hei aromatawai i te pānga o te whakaaturanga o ngā pereti toto ki ngā kukū huka teitei, i konatunatuahia ngā rahinga ōrite (0.5 ml) o te 0%, 5%, 12.5%, 25%, me te 50% huka i roto i te wai ki ngā tauira pereti toto hei whiwhi i te 0%, 2.5%, 6.25%, 12.5% ​​me te 25% kukū o te ranunga huka, ā, i konatunatuahia ngā ngongo ki runga i te rūrū ngongo whakamātautau mō te 15 meneti. I tātarihia te TAC o ia ranunga i roto i ngā tāruatanga e toru i muri i te 15 meneti. I toharitehia te maha o ngā pereti toto (PLT), te maha o ngā toto whero, MCV, me te MPV mō ia ngongo, ā, i tatauhia te toharite o te maha o ngā pereti toto, te maha o ngā toto whero, MCV, me te MPV mō ngā tauira PRP katoa.
I muri i te otinga o te wāhanga tuatahi o te kohikohinga raraunga, i kite mātou i te pikinga nui o te rahinga o ngā pereti toto i roto i ngā pereti toto PRP i muri i te tāpiritanga o te D50W. Kāore e tohu ana ngā pereti toto PRP i ngā pereti toto katoa i roto i te toto, ā, he rerekē te reo PRP i te reo WB. Nō reira, i whakatau mātou ki te whakahaere i tētahi whakamātautau wāhanga tuarua mō te pānga o te tāpiritanga o te D50W ki te toto katoa.
Mō te wāhanga tuarua, i whiriwhiria e mātou he rahi tauira o te 30 i runga i ngā hua o te raupapa tuatahi, e ai ki te whakaahuatanga i te wāhanga Tātari. I roto i tēnei raupapa, e 20 ngā tūao i tuku tauira toto (Ripanga 1). I tangohia te toto katoa (1.8 ml) ki roto i tētahi pūwero 3 ml, ā, i tāpirihia he 0.2 ml 40% NaCl hei ārai i te toto totoka. I konatunatua te pūwero toto katoa mō te rima hēkona me te mīhini whakaranu vortex, ā, i tātarihia te CBC i roto i ngā tāruatanga e toru. I muri i te tātari, i tāpirihia te toto ārai totoka ki te 2 ml o te 50% hukahuka i roto i tētahi pūwero 5 ml (ko te kukū hukahuka whakamutunga he tata ki te 25% (D25) ā, i whakanohoia ki roto i tētahi ngongo rūrū mō te 30 meneti. I muri i te 30 meneti, i tātarihia te D25/CBC i roto i ngā pūwero WB i roto i ngā tāruatanga e toru. I toharitehia te maha o ngā pereti toto, te maha o ngā RBC, te MCV, me te MPV mō ia pūwero, ā, i tatauhia te toharite o te PLT, te maha o ngā RBC, te MCV, me te MPV mō ia tauira i mua atu me muri i te tāpiri i te hukahuka.
Nā te mea he maha ngā wā ka pāngia ngā pereti toto katoa e te huka toto toto tiketike i te wā e rongoatia ana te huka toto nui nā te werohanga iti rawa, ā, ehara i te mea noa te whakakotahi i te PRP me te huka toto toto tiketike i mua tata o te werohanga, i whakatau mātou ki te ako i te huka toto toto tiketike me te WB i te Wāhanga 1. Hipanga Tuatoru me te tuawhā. I ia wāhanga, e 20 ngā tūao i koha i te 7-8 ml o te ACD-A (waikawa kei roto te trisodium citrate (22.0 g/l), te waikawa citric (8.0 g/l) me te huka toto (24.5 g/l), te otinga dextrose citrate) hei rongoā whakaheke toto (Ripanga 1). Ko ngā ranunga huka toto nui atu i te 12.5% ​​anake i whakamahia hei whakatau i te ōrau paepae e pā ana ki te pikinga o te MPV. I te wāhanga tuatoru, ka whakanohoia te 1 ml o te toto ki roto i te ngongo whakamātautau. Kātahi ka whakaranua te toto ki runga i te mīhini whakaranu vortex mō te 10 hēkona mā te tāpiri i te 1 ml o te 30% hukahuka, 40% hukahuka, 50% hukahuka rānei ki te ngongo kia whiwhi ai i te kukū hukahuka whakamutunga o te 15%, 20%, me te 25%, ia. I tātarihia ngā tauira toto hukahuka mō te CBC i muri tonu i te whakaranu, ā, i tuaruatia i ia rua meneti mō te 30 meneti.
I te wā o te whakaranu tuatahi, mā te tāpiri i te huka toto hypertonic 1:1 me te WB, te PRP rānei, ka whakaatuhia ngā pereti toto ki ngā kukū neke atu i te 25% mō ētahi hēkona. I te taahiraa tuawhā, hei aromatawai i te pānga o te huka toto hypertonic me te iti rawa o ngā kukū tihi tīmatanga, me te whakamātautau i te rohe o runga o te pānga o te huka toto, i tāpirihia e mātou he iti noa te toto ki te D25W, ki te D50W rānei. Whakatakotoria he 1 ml o te D25W, ki te D50W rānei ki roto i tētahi ngongo, ka tāpirihia he 0.2 ml o te WB i a koe e hurihuri ana i te tauira mō te 10 hēkona. I ēnei wā, i whakaatuhia te toto ki te huka toto i te kukū tata ki te 20% i runga ake i te kukū whakamutunga, kaua ki te 50% i runga ake i te kukū whakamutunga pērā i te Wāhanga 3, ā, ka hua ake he kukū huka toto whakamutunga o te 20.8% me te 41.6%. I tātarihia ngā tauira whakaranu i te wā kotahi i te taahiraa 3.
I te taahiraa tuatahi o ia raupapa whakamātotoru huka, e 30 ngā tauira i tangohia nā te mea koinei te rahi tauira e tika ana mō te rangahau whakamātautau [9]. I te mutunga o ia wāhanga (tae atu ki te wāhanga tuatahi), aromatawaihia te rawaka o te rahi tauira mā te whakamahi i te tātai i whakamahia hei whakatau i te rahi tauira e hiahiatia ana hei whakatau i te toharite o te taurangi putanga tonu i roto i te taupori kotahi. Tātai n = Z2 x SD2 /E2. I roto i tēnei whārite, ko Z te kaute-Z, ko SD te paerewa rerekētanga, ā, ko E te hapa e hiahiatia ana [10]. Ko tā mātou alpha he 0.05, e rite ana ki te uara Z o te 1.96, ā, e tumanakohia ana he hapa o te 5 (i roto i te ōrau). Nō reira ka whakatauhia e mātou mō n = (1.962 x SD2)/52. I whakaatuhia e ngā hua he iti ake te rahi tauira e hiahiatia ana mō ia wāhanga i te tau tuturu i kohia.
I ngā wā 1, 3 me te 4 mā te whakamahi i te neke atu i te kotahi te kukū huka, i tātarihia te pānga o ngā kukū huka rerekē mā te whakatairite i te huringa haurua i waenga i te wā 0 me ia wā i muri mai (wāhanga 1 i te 15 meneti, wā 3 i te 15 meneti). me te whā i te 15 hēkona, kātahi i ia rua meneti.) I whakatairitehia ngā reiti huringa mō ia wā mā te whakamahi i te whakamātautau-U Mann-Whitney nā te mea kāore ngā raraunga i whai i te tohatoha noa i whakatauhia e te whakamātautau noa Shapiro-Wilk. Mai i te mea i mahia he tātari 1-ki-1 o ētahi rōpū (rima) i ngā taahiraa tuatahi, tuatoru me te tuawhā (rima katoa), i mahia he whakatikatika Bonferroni hei whakatika i te uara alpha e hiahiatia ana ki te ≤0.01 engari kāore i te ≤0.05.
Te whakahekenga o te tatau pereti toto me ngā kukū katoa o te dextrose hypertonic me te pikinga o te MPV i roto i ngā pereti toto PRP i te kukū dextrose >12.5%: Kua piki ake te tatau pereti toto PRP mai i te kotahi ki te rima ngā wā kukū i whakaritea ki te toto katoa taketake, he rerekē i runga i te tikanga (kāore i whakaaturia). Te whakahekenga o te tatau pereti toto me ngā kukū katoa o te dextrose hypertonic me te pikinga o te MPV i roto i ngā pereti toto PRP i te kukū dextrose >12.5%: Kua piki ake te tatau pereti toto PRP mai i te kotahi ki te rima ngā wā kukū i whakaritea ki te toto katoa taketake, he rerekē i runga i te tikanga (kāore i whakaaturia). Уменьшение количества тромбоцитов при всех концентрациях гипертонической декстрозы и увеличение MPV i roto i te тромбRPо цитрациях декстрозы > 12,5%: количество тромбоцитов PRP увеличилось в 1-5 раз по сравнению с исходной цельнойто кровьзаю, томид (kahore he korero). Te hekenga o te maha o ngā pereti toto i ngā kukū dextrose hypertonic katoa, me te pikinga o te MPV i roto i ngā pereti toto PRP i te >12.5% ​​​​kukū dextrose: Kua piki ake te maha o ngā pereti toto PRP 1-5 ngā wā i whakaritea ki te toto katoa taketake, i runga i te tikanga (kāore i whakaaturia). ).在> 12.5% ​​的葡萄糖浓度下，所有浓度的高渗葡萄糖降低血小板计数，PRP 血小MP板中增加：与基线全血相比，PRP 血小板计数从浓度的1 倍上升到5 倍，因方法而异／／法而异／。 I te kukū huka >12.5%, ka whakaitihia te maha o te toto e te kukū huka nui, ka piki ake te MPV toto PRP: ki te whakatauritea ki te 与基线全血, ka piki ake te maha o te toto PRP mai i te 1 ki te 5 ngā wā i te kukū (kāore i whakaahuatia). При концентрациях глюкозы >12,5% все концентрации гипертонической глюкозы снижали количество тромбо MPVлив, тромбоцитах PRP: количество тромбоцитов PRP увеличивалось mai i te 1- ki te 5-кратных концентраций по сравнению с сисхотдрация цельной крови, в зависимости от метода (не описано ). I ngā kukū huka toto >12.5%, i heke te maha o ngā pereti toto i ngā kukū huka toto toto tiketike katoa, ā, i piki ake te MPV i roto i ngā pereti toto PRP: i piki ake te maha o ngā pereti toto PRP 1 ki te 5 ngā wā i whakaritea ki ngā kukū toto katoa taketake, i runga i te tikanga (e ai ki te whakaahuatanga).E whakaatu ana te Pikitia 1 i heke te maha o ngā pereti toto mā te tata ki te 75% i muri i te waimehatanga ki te wai, ā, mā te 20-30% i muri i te 15 meneti o te waimehatanga me ngā kukū huka rerekē i whakaritea ki te PRP taketake me te waimehatanga 1:1 i whakatikatikaina mō te rōrahi (1-k1 me te whakatikatika rōrahi). te whakaputa uri k-1).1 te whakaputa uri).
Ko te maha o ngā pūtau i roto i ia waimehatanga ka whakaatuhia hei hautanga o te maha taketake i mua i te waimehatanga.
I heke iti noa te MPV i te wā o te whakaputanga PRP, me te kore he panonitanga anō o ngā kukū waimeha ki te 12.5% ​​​​i roto i te wai, i te hukahuka rānei (tae atu ki te 25% o ngā ranunga hukahuka PRP) ā, i piki ake mā te neke atu i te 20% i muri i te waimehatanga ki roto i te otinga hukahuka 50% (Pikitia .2). He rerekē, kāore i whakaatuhia e ngā toto whero he panonitanga nui o te rōrahi i tētahi waimehatanga i tua atu i te H2O.
Ko te rōrahi toharite o ngā pūtau i roto i ia waimehatanga ka whakaaturia hei ōrau o te rōrahi taketake i mua i te waimehatanga.
I kitea he whakahekenga rite tonu o te maha o ngā pereti toto me te pikinga o te CVR i roto i te BC i whakararuraruhia ki te 50% te hukahuka (hei hanga me te 25% te hukahuka). E whakataurite ana te Ripanga 2 i te maha o ngā pūtau me te rahinga o ngā pūtau i roto i te toto katoa i whakaranua ki te 50% te dextrose me ngā raraunga PRP wāhanga 1 i whakaranua ki te 50% te dextrose. Kāore i kitea ngā huringa o te maha o ngā toto whero me te MCV o te toto whero, ā, ehara i te mea i arotahihia e mātou.
SD = paerewa rerekētanga, MD = rerekētanga toharite i waenga i ngā rōpū, SE = paerewa rerekētanga o te rerekētanga toharite, RBC = erythrocytes, PLT = platelets, PRP = plasma whai platelet, WB = toto katoa
I muri i te tāpiritanga o te D50W ki te WB, ko te ōrau o te ngaronga o te pereti toto i whakatikatikaina ki te waimehatanga he 7.7% (310±73 vs. 286±96) ki te whakarite ki te 17.8% mō te waimehatanga PRP i roto i te D50W (664±348 vs. 544±277). I piki ake te WB MPV mā te 16.8% (mai i te 10.1 ± 0.5 ki te 11.8 ± 0.6), i te mea i piki ake te PRP MPV mā te 26% (9.2 ± 0.8 vs. 11.6 ± 0.7). Ahakoa he nui ake ngā rerekētanga toharite i roto i te whakahekenga o te tatau pereti me te pikinga o te MPV me te PRP, tata nui ngā huringa o te whakahekenga o te tatau pereti i roto i te WB (310 ± 73 ki te 286 ± 96 (-7.7%); p = .06) ā, he nui te pikinga o te MPV (10.1 ± 0.5 ki te 11.8 ± 0.6 (+16.8) p < .001). Ahakoa he nui ake ngā rerekētanga toharite i roto i te whakahekenga o te tatau pereti me te pikinga o te MPV me te PRP, tata nui ngā huringa o te whakahekenga o te tatau pereti i roto i te WB (310 ± 73 ki te 286 ± 96 (-7.7%); p = .06) ā, he nui te pikinga o te MPV (10.1 ± 0.5 ki te 11.8 ± 0.6 (+16.8) p < .001).Ahakoa he nui ake ngā rerekētanga toharite i roto i te whakahekenga o te tatau pereti toto me te pikinga o te CVR me te PRP, he tata nui ngā huringa o te whakahekenga o te tatau pereti toto i roto i te WB (310 ± 73 ki te 286 ± 96 (-7.7%); p = 0.06).увеличение MPV было значительным (mai 10,1 ± 0,5 ki 11,8 ± 0,6 (+16,8) p <0,001). he nui te pikinga o te MPV (mai i te 10.1 ± 0.5 ki te 11.8 ± 0.6 (+16.8) p < 0.001).尽管PRP 在血小板计数减少和MPV 增加方面的平均差异显着更大，但WB内血小板计数减少的变化几乎是显着的（310 ± 73 至286 ± 96 (-7.7%)；p = .06）和MPV的增加是显着的（10.1 ± 0.5 到11.8 ± 0.6 (+16.8) p < .001）。尽管 PRP 在 血小板 计数 和 和 增加 方面 的 平均 差异 显着 大 ， 但 但 内杀小的 几乎 是 显着 的 （（（310 ± 73 至 286 ± 96 (-7.7%) ； p = .06）和MPV 的增加是显着的（1 ± 8. 0.6 (+16.8) p <.001）.Tata nui te panonitanga o te whakahekenga o te tatau pereti toto i roto i te WB (mai i te 310 ± 73 ki te 286 ± 96 (-7.7%); p = 0.06), ahakoa he nui ake ngā rerekētanga toharite o te PRP i roto i te whakahekenga o te tatau pereti toto me te pikinga o te MPV, ā, he nui te pikinga o te MPV.(mai 10,1 ± 0,5 ki 11,8 ± 0,6 (+16,8) р <0,001). (mai i te 10.1 ± 0.5 ki te 11.8 ± 0.6 (+16.8) p < 0.001).
I hiahiatia he kukū whakamutunga o te 20% huka kia kite i te huringa nui o te MPV, engari i kitea ake te huringa o te MPV i te kukū whakamutunga o te 25%. I pumau te ngaronga o te pereti i muri i te hekenga tuatahi. I kite mātou i te hekenga koi tuatahi o te CVR, heoi, i tere te whakahokinga mai o te CVR i te kukū huka whakamutunga o te 25%, he nui ake i ngā taumata CVR i kitea i ngā kukū huka whakamutunga o te 20% me te 15% (Pikitia 3 me te taha maui o te Ripanga 3; pouaka atarangi). e tohu ana i ngā uara-p ≤ alpha me te whakatikatika Bonferroni o te 0.01). I kitea anō hoki he hekenga koi tuatahi o te maha o te PLT, i kitea i te wāhanga tuatahi o te 0-15 hēkona, kātahi ka noho pumau (mai i te 15 hēkona ki te 30 meneti; taha maui o te ripanga 4).
Nā te tāpiritanga o ngā kukū huka rerekē ki te toto katoa i hua ake ai te hekenga tere tuatahi o te MPV, ā, i muri mai ka neke atu i te 20% te whakaoranga e whakawhirinaki ana ki te kukū. E whakaatu ana te kōrero i te kukū o te huka i muri i te waimehatanga. I mahia te D15, D20 me te D25 i roto i te waimehatanga 1:1. I mahia te D21 me te D41 i te waimehatanga 1:5.
E whakaatu ana te Ripanga 4 i te panonitanga o te tatau pereti ina whakatōngia ki te huka toto toto tiketike. I kitea e mātou he whanaungatanga e whakawhirinaki ana ki te horopeta i waenga i te hekenga tonu o ngā tau PLT i te whakatōngia 1:1 me te whakatōngia 1:5. I te whakataurite i ngā whakatōngia 1:1 hei rōpū kotahi me ngā whakatōngia 1:5, he iti iho te hekenga tonu o te tatau pereti o te rōpū 1:1 i te rōpū 1:5 66±48,000 (23%) ki te 99±69,000 (37%). , p = 0.014) i roto i te rōpū 1:5. Whai muri i te hekenga tuatahi i te pūwāhi ine tuatahi, kua pumau te tatau pereti hei ōrau o te huka toto (Pikitia 4).
Ina tāpirihia te toto katoa ki te hukahuka i te ōwehenga 1:1, ka heke te maha o ngā pereti toto mā te 25%. Heoi, i te tāpirihia o te toto katoa i te ōwehenga 1:5, he nui ake te hekenga – tata ki te 50%.
I tere ake, ā, i tino nui ake te whakanui ake o te MPV i te 25%, i te 21% rānei, nā te 41% te huka. Kei te Pikitia 3 ngā hua o te MPV. I ngā whakakōkī katoa, kāore i kitea he hekenga tuatahi tonu o te MPV i muri i te tāpiritanga o te 50% huka. I te whakamahinga o te 25% huka (te kukū huka 20.8% i te whakakōkī whakamutunga), he rite te huringa o te MPV ki te huringa o te 20% huka i te whakakōkī 1:1 (Pikitia 3). Ahakoa i te tīmatanga he nui ake ngā huringa o te MPV i te kukū whakauru 41% i te 25%, kāore i te nui te rerekētanga o te MPV i waenga i te 41% me te 25% i muri i te 16 meneti (Ripanga 3, matau). He mea whakamere hoki ko te 25% huka i whakanui ake i te MPV he pai ake i te 20.8%.
I whakaūhia tētahi wāhanga o tēnei rangahau in vitro tā mātou whakapae. I whakaatuhia te pakaru ā-wāhanga pea o ngā pereti toto mā te ranunga o te dextrose, te tere o te whakaurunga o ngā pereti toto ki te hypertonicity tino nui, me te pikinga nui o te MPV hei whakautu ki te > 25% o ngā kukū o te hypertonic dextrose. I whakaatuhia te pakaru ā-wāhanga pea o ngā pereti toto mā te ranunga o te dextrose, te tere o te whakaurunga o ngā pereti toto ki te hypertonicity tino nui, me te pikinga nui o te MPV hei whakautu ki te > 25% o ngā kukū o te hypertonic dextrose. Он показал потенциальный частичный лизис тромбоцитов примесью декстрозы, быструю аккомодацию тромбоцэтогостов д гипертонуса и значительное повышение MPV в ответ на гипертоническую концентрацию декстрозы > 25%. I whakaatuhia te kaha o te pakaru ā-wāhanga o ngā pereti toto me te dextrose, te tere o te noho o ngā pereti toto ki te tino hypertonicity, me te pikinga nui o te MPV hei whakautu ki ngā taumata dextrose hypertoni >25%.它显示出通过葡萄糖混合物潜在的部分血小板溶解，血小板快速适应极端，付应极端，付板溶解。浓度的高渗葡萄糖时MPV 显着上升。它 显示 出 通过 葡萄糖 潜在 的 部分 血小板 溶解 血小板 快速 适应 极端 ，响应> 25% 浓度 高渗 葡萄糖 时 时 mpv 显着。。。。 Он показывает потенциальный частичный лизис тромбоцитов смесями с глюкозой, быструю адаптацию тромболиктов гипертонусу и значительное увеличение MPV в ответ на концентрацию гипертонической глюкозы > 25%. E whakaatu ana i te pakaru ā-wāhanga pea o ngā pereti toto e ngā ranunga hukahuka, te urutau tere o ngā pereti toto ki te hypertonicity tino nui, me te pikinga nui o te MPV hei whakautu ki te huka toto hypertoni >25%.Ko te pikinga tuatahi i tino teitei i te 41.6% o te pānga ki te huka, engari ko te pikinga o te MPV i tata ki te 25% o te pānga ki te huka tata ki te 20 meneti i muri i te pānga.
Ka pāngia te kukū o ngā pereti toto e te hukahuka. I kite mātou kua heke te nui o te PLT i ngā waimehatanga katoa o te hukahuka. Ko te hekenga nui o te maha o ngā pereti toto i roto i ngā waimehatanga H2O (0%) o te raupapa PRP ka hono pea ki te pakarutanga osmotic. Heoi anō, he mea hanga pea tēnei nā te piri o ngā pereti toto, engari he rerekē tēnei ki te kore o te huringa MPV i tēnei waimehatanga. Ko te tikanga o tēnei kitenga he tino tairongo ētahi pereti toto ki te hypoosmolarity.
I roto i ngā whakaranunga 1:1 katoa o te hukahuka, i heke te nui o te PLT mā te 20-30%, tae noa ki te D5W (hypotonic i te 252 mOsm), e tohu ana pea i tētahi pānga motuhake kore-osmotic o te hukahuka, nā te mea i noho tonu te PLT me te MPV i te pikinga toru-whakarea o te kukū. hukahuka. mai i te D5W ki te D25W. Inaa, i piki haere ngā kukū PLT i te pikinga o te osmolarity.
Ko te hekenga o te PLT i waenga i ngā waimehatanga 1:1 me te 1:5, ko te tikanga ko te pānga waimeha e whakawhirinaki ana ki te kukū huka tuatahi me te kukū huka whakamutunga. Mena i whakawhirinaki noa ki te kukū huka tuatahi, ka kitea he rerekētanga i roto i te whakahekenga PLT i waenga i ngā kukū huka 1:1. Engari kāore mātou. Mena ka whakawhirinaki noa te pānga waimeha ki te kukū huka whakamutunga, kāore mātou e tumanako ki te nui o te rerekētanga i waenga i te waimehatanga 20% 1:1 me te waimehatanga 20.8% 1:5. Heoi anō, i taea e mātou.
Mena ka ngaro te pereti toto nā te pakaru o te pereti toto, ka puta he lysate wāhanga, ā, muri iho ka tukuna ngā cytokine me ngā mea whakatipu ki te taiao o waho o te pūtau. He maha ngā rangahau kua whakaatu he tata rite te whai huatanga o te lysate pereti toto ki te PRP me te otinga whakatipu [11]. Kua whakaatuhia te PRP he otinga whai hua mō te rongoā i te whakatipu [12-14].
Ka rere ngā pereti toto kore mahi i roto i te āhua o tētahi kōpae kua whakapakaritia me ētahi hanganga o roto. I te wā e whakahohehia ana, ka huri hei porowhita, hei amoeba rānei, ka hua ake he pikinga o te rōrahi. Ko te pikinga o te rōrahi me whakanui ake i te horahanga o te mata, koinei te hua o te putanga mai o te pūnaha ngongo tuwhera (OCS) me te tāpiri o ngā kirikiri exocytic ki te kiriuhi. Kāore anō kia whakatauhia mēnā ko te pikinga o te MPV i puta mai i te huka toto hypertonic e pā ana ki tētahi, ki ngā tikanga e rua rānei, engari mēnā ko te mea whakamutunga, ko te pikinga o te MPV e tohu ana i te degranulation.
I whakaatuhia e tēnei rangahau ko te pānga o te nui o te huka i runga i te PRP, i ngā pereti toto katoa rānei, i hua ake ai te pikinga o te MPV i roto i ngā meneti 15, me te kukū huka o te 25% me te 41.6%, ia.
Tērā pea nā te whānui haere o ngā hononga microtubule e karapoti ana i te pikinga o te MPV o ngā pereti toto i puta ai te pikinga ake hei whakautu i te urunga mai o te konupūmā. Kua whakaaturia e Liu et al. te āwhina a te huka ki te whakahaere i te urunga mai o te konupūmā mā te hongere TRPC6 o ngā pereti toto [6]. Ko tā mātou whakapae ko te huka ka whakangāwari i ngā hononga microtubule, ka piki ake te MPV me te whakaaweawe me te whakahohenga rānei o ngā pereti toto. Heoi, ki te whakatau i ā mātou hua, he wāhanga noa iho tēnei o te kōrero. I roto i ā mātou whakamātautau, kāore he kukū i raro i te D25W i hua ake ai te pikinga o te MPV. I te mea kāore anō mātou kia whakamātautau i te pānga ki ngā kukū huka i waenga i te 12.5% ​​​​me te 25%, e kī ana ā mātou hua wāhanga 1 tera pea he paepae i roto i tēnei awhe o ngā kukū huka e arahi ana ki te pikinga o te MPV. I whakaatuhia e ngā whakamātautau anō i ngā wāhanga 3 me te 4 ko te 20-25% te huka te paepae mō tēnei, engari kāore anō kia mārama he aha.
I kitea anō hoki e mātou he hekenga o te MPV ~9% i muri i te centrifugation. Kāore i te mārama mēnā ko tēnei hekenga o te MPV nā te mea he nui ake, he kikī ake ngā pereti toto kua mau ki roto i te paparanga RBC o te centrifuge. Tērā pea he mea nui tēnei kitenga ki ngā tākuta hauora nā te mea tera pea e tohu ana ko ngā pereti toto PRP he wāhanga iti ake, he iti ake te kikī o ngā pereti toto WB.
I roto i tētahi rangahau o mua, i whakaaturia e mātou he iti te utu mō te whakarite PRP mā ngā tikanga ā-ringa [8]. Mena ka whakaaweawe te huka i ngā pereti kiko, i te PRP rānei, ka ngawari ake te whakahohe, mena ka puta te PRP me ngā āhuatanga lysate wāhanga, ka taea e tēnei te whakarei ake i te whakahoutanga me te whakaiti i te hiahia mō te rongoā. Nō reira, ko te whakakotahitanga o te PRP me te huka tino kukū he iti ake te utu i te PRP, i te huka anake rānei.
He maha ngā ngoikoretanga o tā mātou rangahau. Tuatahi, ka whakamahia e mātou te PRP i ahu mai i ngā tikanga rerekē. Ka taea e tēnei te arahi ki ngā hua taupatupatu. Tuarua, kāore i taea e mātou te whakahaere i tētahi tātaritanga matū koiora o tētahi o ā mātou tauira kia tika ake ai te whakatau mēnā kua puta te whakahohenga o te pereti toto. E hiahia ana mātou ki te ine i te P-selectin, te pereti toto 4, ngā whakakotahitanga pereti toto monocytic, ētahi atu tohu rānei o te whakahohenga pereti toto kia pai ake ai te mārama ki te nui, te aroaro rānei o te degranulation o te alpha granule, engari kei tua atu tēnei i te whānuitanga o tēnei rangahau. Tuatoru, kāore i taea e mātou te whakaū mā te karu hikohiko, mā ētahi atu tikanga rānei, ko te pikinga o te MPV i roto i ngā pereti toto e kitea ana te hukahuka i puta mai i te pānga ki ngā raranga microtubule.
Nā ngā ranunga o te WB, o te PRP rānei me te 25% o te huka i whakapiki ake i te MPV, e tohu ana i te tīmatanga o te whakahohenga o ngā pereti toto, ahakoa kāore i whakaaturia e tēnei rangahau te haere whakamua o te whakakotahitanga, te whakahekenga rānei. Nā te ranunga huka toto hypertonic i ngaro ai ngā pereti toto, tera pea he pānga lytic. Ka taea e te whakahohenga wāhanga, te pakaru rānei o ngā pereti toto te whakaputa i te whakahoutanga o te kiko i muri i te werohanga o ngā pereti toto. Kāore i te mārama he aha ngā hua haumanu ka puta mai i ēnei huringa. Kua whakaatuhia e ētahi atu rangahau he ine tika ake o te whakahohenga, te pakaru rānei, ā, kua aromatawaihia ngā pānga haumanu rerekē o ngā ranunga huka toto hypertonic me te WB, te PRP rānei.
He rongoā whakaora ngāwari, he iti hoki te utu mō te whakanui huka toto, e tere haere ana te horapa, e tautoko ana hoki i ngā rangahau haumanu. E whakaatu ana tēnei rangahau i tētahi tikanga ā-tinana, ki te whakaūtia, ka taea te āwhina i a tātou ki te mārama ki tētahi wāhanga o te tikanga whakaora o te rongoā whakatupu.
Ngā Pūtaiao Hauora me te Koiora i Te Whare Wānanga o Missouri, Kura Rongoā o Kansas City, Kansas City, USA
Ngā Kaupapa Tangata: I whakaae, i whakaae rānei ngā kaiuru katoa o tēnei rangahau. Kua tukuna e te International Society for Cellular Medicine te whakaaetanga ICMS-2017-003. Kua whakaaetia te kawa e whai ake nei mō te whakamahinga anō e te Poari Arotake Whare o te International Society for Cellular Medicine: Taitara: Te tatau i te hua rongoā plasma whai-pereti i runga i te tatau pereti CBC turanga. Ngā Kaupapa Kararehe: I whakaū katoa ngā kaituhi kāore he kararehe, he kiko rānei i uru ki tēnei rangahau. Ngā Taupatupatu Pānga: E ai ki te Puka Whakaaturanga Kotahi ICMJE, e whakaatu ana ngā kaituhi katoa i ēnei e whai ake nei: Ngā mōhiohio utu/ratonga: E whakaatu ana ngā kaituhi katoa kāore rātou i whiwhi tautoko pūtea mai i tētahi whakahaere mō te mahi i tukuna. Ngā Hononga Pūtea: E whakaatu ana ngā kaituhi katoa kāore ā rātou hononga pūtea i tēnei wā, i roto rānei i ngā tau e toru kua pahure ake nei, me tētahi whakahaere e hiahia ana ki te mahi i tukuna. Ētahi atu Hononga: E whakaatu ana ngā kaituhi katoa kāore he hononga pūtea, he mahi rānei e pā ana ki te mahi i tukuna.
Harrison TE, Bowler J, Reeves K et al. (Mei 17, 2022) Te pānga o te huka ki te tatau me te rahinga o ngā pereti toto: ngā pānga ki te rongoā whakaora. Cure 14(5): e25081. doi:10.7759/cureus.25081
© Mana pupuri © 2022 Harrison et al. He tuhinga urunga-tuwhera tēnei e tohatohahia ana i raro i ngā tikanga o te Raihana Whakawhiwhinga Creative Commons CC-BY 4.0. E whakaaetia ana te whakamahinga, te tohatoha, me te tārua mutunga kore i roto i tētahi momo pāpāho, mena ka whakaingoatia te kaituhi taketake me te pūtake.