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Ko te toto kore e whakahaeretia tētahi o ngā tino take o te mate. Mā te tere o te hemostasis ka ora tonu te tangata hei āwhina tuatahi i te wā o te pakanga, ngā aituā waka, me ngā mahi whakaiti mate. Ka taea e te hanganga hiato muka nanoporous (NFRCS) i ahu mai i tētahi hanganga hanga kiriata hemostatic māmā (HFFC) hei wāhanga tonu te whakaoho me te whakarei ake i te hemostasis. Ko te whanaketanga o te NFRCS e hangai ana ki te hoahoa o te parirau o te pepeke. Ko te hanganga parirau pepeke he parirau whakawhiti me te parirau roa, ā, ka honoa ngā kiriuhi parirau tetahi ki tetahi hei pupuri i te pono o te hanganga moroiti. Ka pania ōritetia e te HFFC te mata o te muka ki tētahi kiriata matotoru nanometer, ā, ka hono i te matotoru miro tohatoha matapōkere (Ct) (wāhanga marara) hei hanga i tētahi hanganga nanoporous. Mā te whakakotahitanga o ngā wāhanga tonu me te marara ka whakaiti i te utu o te hua mā te tekau ngā wā ki te whakataurite ki ngā hua e wātea ana i te hokohoko. Ka taea te whakamahi i te NFRCS whakarerekētia (tampons, ringi ringa rānei) i roto i ngā momo tono hauora. Kua whakatauhia e ngā rangahau in vivo ko te Cp NFRCS kua whakawhanakehia ka whakaoho, ka whakarei ake hoki i te tukanga totoka i te wāhi i whakamahia ai. Ka taea e te NFRCS te whakarerekē i te taiao moroiti me te mahi i te taumata pūtau nā tōna hanganga nanoporous ka hua ake he pai ake te whakaora patunga i roto i te tauira patunga tapahi.
Ko te toto kore e whakahaeretia i te wā o te pakanga, i te wā o te pokanga me ngā āhuatanga ohorere ka waiho hei mōrearea nui ki te ora o te hunga whara1. Ka arahi atu ēnei āhuatanga ki te pikinga whānui o te ātete toto ā-taha, ka arahi ki te ohorere toto. Ko ngā mahi tika hei whakahaere i te toto i te wā me muri i te pokanga e kiia ana he mea morearea ki te ora2,3. Ko te kino o ngā toto nui ka arahi ki te ngaronga toto nui, ka hua ake he reiti mate o ≤ 50% i te pakanga me te 31% i te wā o te pokanga1. Ko te ngaronga toto nui ka arahi ki te hekenga o te rahinga o te tinana, ka whakaiti i te putanga o te ngakau. Ko te pikinga o te ātete toto ā-taha katoa me te ngoikore haere o te tohanga toto iti ka arahi ki te hypoxia i roto i ngā whekau tautoko ora. Ka puta pea te ohorere toto mena ka haere tonu te āhuatanga me te kore he wawaotanga whai hua1,4,5. Ko ētahi atu raruraru ko te haere whakamua o te hypothermia me te acidosis metabolic, me te mate toto toto e aukati ana i te tukanga toto toto. Ko te ohorere toto nui e hono ana ki te mōrearea nui ake o te mate6,7,8. I roto i te ohorere taumata III (haere whakamua), he mea nui te whakawhiti toto mō te oranga o te tūroro i te wā o te mate me te mate i te wā o te pokanga me muri i te pokanga. Hei whakakore i ngā āhuatanga morearea katoa o runga ake nei, kua whakawhanakehia e mātou he tūāpapa hiato muka-whakapakari nanoporous (NFRCS) e whakamahi ana i te kukū polymer iti rawa (0.5%) mā te whakamahi i te huinga o ngā polymer hemostatic wairewa-wai.
Mā te whakamahi i te muka whakakaha, ka taea te whakawhanake i ngā hua utu-pai. He rite ngā muka kua whakaritea matapōkeretia ki te hanganga o te parirau o te pepeke, e taurite ana e ngā rārangi whakapae me ngā rārangi poutū i runga i ngā parirau. Ka hono ngā uaua whakawhiti me ngā uaua roa o te parirau ki te kiri parirau (Pikitia 1). Ko te NFRCS he mea hanga mai i te Ct whakakaha hei pūnaha tūāpapa me te kaha ā-tinana me te ā-mīhini pai ake (Pikitia 1). Nā te utu me te mahi toi, he pai ake ki ngā taote pokanga te whakamahi i ngā ine miro miro (Ct) i te wā e pokanga ana me te takai. Nō reira, i runga i ōna painga maha, tae atu ki te > 90% te cellulose tioata (e whai wāhi ana ki te whakarei ake i te mahi hemostatic), i whakamahia a Ct hei pūnaha angaanga o te NFRCS9,10. Nō reira, i runga i ōna painga maha, tae atu ki te > 90% te cellulose tioata (e whai wāhi ana ki te whakarei ake i te mahi hemostatic), i whakamahia a Ct hei pūnaha angaanga o te NFRCS9,10. Следовательно, учитывая его многочисленные преимущества, в том числе > 90% кристаллической целлюлозы (участивулозы) гемостатической активности), Ct использовали в качестве скелетной системы NFRCS9,10. Nō reira, i runga i ōna painga maha, tae atu ki te >90% o te cellulose tioata (e whai wāhi ana ki te pikinga o te mahi hemostatic), i whakamahia a Ct hei pūnaha angaanga NFRCS9,10.因此，考虑到它的多重益处，包括> 90% 的结晶纤维素（有助于增强止血活性Ｋ，NF0000的骨架系统。因此，考虑到它的多重益处，包括> 90%Nō reira, i runga i ōna painga maha, tae atu ki te neke atu i te 90% o te cellulose tioata (āwhina ki te whakarei ake i te mahi hemostatic), i whakamahia a Ct hei tūāpapa mō te NFRCS9,10.I pania te Ct ki te mata (i kitea te hanganga kiriata matotoru nano) ā, i honoa ki tētahi hanganga hanga kiriata hemostatic (HFFC). He rite te mahi a te HFFC ki tētahi matrigel, e pupuri ana i te Ct kua whakanohoia matapōkeretia. Ka tukuna e te hoahoa kua whakawhanakehia te ahotea i roto i te wāhanga marara (ngā muka whakapakari). He uaua ki te whiwhi hanganga nanoporous me te kaha miihini pai mā te whakamahi i ngā kukū polymer iti. Hei tāpiri, ehara i te mea ngāwari ki te whakarite i ngā pokepoke rerekē mō ngā tono hauora rerekē.
E whakaatu ana te ahua i tētahi hoahoa o te NFRCS i runga i te hanganga parirau o te pepeke (A). E whakaatu ana tēnei ahua i tētahi whakatauritenga o te hanganga parirau o te pepeke (e hono ana ngā uaua whakawhiti me ngā uaua roa o te parirau) me tētahi whakaahua-whakawhiti o te Cp NFRCS (B). He whakaaturanga hoahoa o te NFRCS.
I whakawhanakehia ngā NFRC mā te whakamahi i te HFFC hei wāhanga tonu hei aro atu ki ngā herenga o runga ake nei. He mea tito te HFFC i ngā momo pūmua hemostatic hanga-kiriata tae atu ki te chitosan (hei pūmua hemostatic matua) me te methylcellulose (MC), te hydroxypropyl methylcellulose (HPMC 50 cp) me te waipiro polyvinyl (PVA)) (125 kDa) hei pūmua tautoko e whakatairanga ana i te hanganga thrombus. Nā te tāpiri o te polyvinylpyrrolidine K30 (PVP K30) i whakapai ake te kaha mimiti makuku o te NFRCS. I tāpirihia te Polyethylene glycol 400 (PEG 400) hei whakapai ake i te hononga pūmua i roto i ngā ranunga pūmua kua herea. E toru ngā hanganga hemostatic HFFC rerekē (Cm HFFC, Ch HFFC me Cp HFFC), arā, ko te chitosan me te MC (Cm), te chitosan me te HPMC (Ch), me te chitosan me te PVA (Cp), i whakamahia ki a Ct. Kua whakaūtia e ngā rangahau whakaahuatanga in vitro me in vivo te kaha whakaora toto me te whakaora patunga o te NFRCS. Ka taea te whakamahi i ngā rauemi hiato e tukuna ana e te NFRCS hei whakarite i ngā momo tūāpapa kia tutuki ai ngā hiahia motuhake.
Hei tāpiritanga, ka taea te whakarerekē i te NFRCS hei takai, hei rōra rānei hei taupoki i te whara katoa o ngā pito o raro me ētahi atu wāhanga o te tinana. Mō ngā whara o ngā peka whawhai, ka taea te whakarerekē i te hoahoa NFRCS kua hoahoatia hei haurua ringa, hei waewae katoa rānei (Pikitia Tāpiri S11). Ka taea te hanga i te NFRCS hei whitiki ringaringa me te kāpia kiko, ka taea te whakamahi hei aukati i te toto mai i ngā whara nui o te ringaringa whakamomori. Ko tā mātou whāinga matua he whakawhanake i tētahi NFRCS me te iti rawa o te polymer ka taea te tuku ki tētahi taupori nui (i raro i te rārangi rawakore) ā, ka taea te whakanoho ki roto i tētahi kete āwhina tuatahi. He māmā, he whai hua, he ōhanga hoki te hoahoa, ka whai hua te NFRCS ki ngā hapori o te rohe, ā, ka taea te whai pānga ki te ao katoa.
I hokona mai te chitosan (taumaha ngota 80 kDa) me te amaranth i Merck, Īnia. I hokona mai te hydroxypropyl methylcellulose 50 Cp, te polyethylene glycol 400 me te methylcellulose i Loba Chemie Pvt. LLC, Mumbai. I hokona mai te waipiro polyvinyl (taumaha ngota 125 kDa) (87-90% hydrolysed) i National Chemicals, Gujarat. I hokona mai te polyvinylpyrrolidine K30 i Molychem, Mumbai, ā, i hokona mai ngā swabs sterile i Ramaraju Surgery Cotton Mills Ltd., Tamil Nadu, me te wai Milli Q (pūnaha purenga wai Direct-Q3, Merck, Īnia) hei kaikawe.
I whakawhanakehia te NFRCS mā te whakamahi i te tikanga lyophilization11,12. I whakaritea ngā hanganga HFFC katoa (Ripanga 1) mā te whakamahi i tētahi whakaoho mīhini. Whakaritea he otinga 0.5% o te chitosan mā te whakamahi i te 1% waikawa acetic i roto i te wai mā te whakaoho tonu i te 800 rpm i runga i tētahi whakaoho mīhini. Ko te taumaha tika o te polymer kua utaina i tohua i roto i te Ripanga 1 i tāpirihia ki te otinga chitosan, ā, i whakaohokia kia whiwhi rā anō i tētahi otinga polymer mārama. I tāpirihia te PVP K30 me te PEG 400 ki te ranunga hua i roto i ngā rahinga i tohua i roto i te Ripanga 1, ā, i haere tonu te whakaoho kia whiwhi rā anō i tētahi otinga polymer matotoru mārama. Ko te kaukau otinga polymer i hua mai i whakamātaohia mō te 60 meneti hei tango i ngā mirumiru hau kua mau mai i te ranunga polymer. E ai ki te Whakaahua Tāpiri S1(b), i tohatohahia te Ct i roto i ia poka o tētahi pereti 6-poka (pokepokea) i tāpirihia ki te 5 ml o te HFFC.
I whakamātauhia te pereti ono-pū mō te 60 meneti kia rite te makuku me te tohatoha o te HFFC ki roto i te whatunga Ct. Kātahi ka whakatiohia te pereti ono-pū ki te -20°C mō ngā hāora 8-12. I whakatiohia ngā pereti mō te 48 hāora kia whiwhi ai i ngā momo hanganga o te NFRCS. Ka whakamahia te tukanga kotahi hei whakaputa i ngā āhua me ngā hanganga rerekē, pērā i ngā tampon, i ngā tampon porotaka rānei, i tētahi atu āhua rānei mō ngā tono rerekē.
Ko te chitosan (80 kDa) (3%) kua paunatia tika ka whakarewaina ki roto i te 1% waikawa acetic mā te whakamahi i te whakaoho aukume. I tāpirihia te 1% PEG 400 ki te otinga chitosan kua puta, ā, i whakaohokia mō te 30 meneti. Ringihia te otinga kua puta ki roto i tētahi ipu tapawhā, tapawhā rānei, ka whakatio ki te -80°C mō te 12 hāora. I whakatōkia ngā tauira kua tio mō te 48 hāora kia puta ai he Cs13 porous.
I whakamātauhia te NFRCS kua whakawhanakehia mā te whakamahi i te Fourier transform infrared spectroscopy (FTIR) (Shimadzu 8400 s FTIR, Tokyo, Japan) hei whakaū i te hototahitanga matū o te chitosan me ētahi atu polymer14,15. I whiwhihia ngā spectra FTIR (te whānui o te whānuitanga spectral mai i te 400 ki te 4000 cm-1) o ngā tauira katoa i whakamatautauria mā te mahi i ngā matawai 32.
I aromatawaihia te tere mimiti toto (BAR) mō ngā whakatakotoranga katoa mā te whakamahi i te tikanga i whakaahuahia e Chen et al. 16 me ngā whakarerekētanga iti. I whakamaroketia ngā NFRK kua whakawhanakehia o ngā hanganga katoa i roto i te oumu korehau i te 105°C i te pō hei tango i te toenga whakarewa. I whakanohoia te 30 mg NFRCS (taumaha tauira tuatahi – W0) me te 30 mg Ct (mana whakahaere pai) ki roto i ngā rihi motuhake kei roto he ranunga o mua o te 3.8% konutai citrate. I ngā wā kua whakaritea, arā, 5, 10, 20, 30, 40 me te 60 hēkona, i tangohia ngā NFRCS, ā, i horoia ō rātou mata i te toto kāore i mimiti mā te whakanoho i ngā tauira ki runga i te Ct mō te 30 hēkona. I whakaarohia te taumaha whakamutunga o te toto i mimitihia e te NFRCS 16 (W1) i ia wā. Tātaihia te ōrau BAR mā te whakamahi i te tātai e whai ake nei:
I whakatauhia te wā toto totoka (BCT) e ai ki tā Wang et al. 17. Ko te wā e hiahiatia ana kia totoka te toto katoa (toto kiore kua konatunatua ki te 3.8% sodium citrate) i te aroaro o te NFRCS i tatauhia hei BCT o te tauira whakamātautau. I whakanohoia ngā wāhanga NFRCS (30 mg) ki roto i ngā ipu taupoki tīwiri 10 ml, ā, i whaowhia ki te 37°C. I tāpirihia te toto (0.5 ml) ki te ipu, ā, i tāpirihia te 0.3 ml o te 0.2 M CaCl2 hei whakahohe i te totoka. Hei whakamutunga, hurihia te ipu i ia 15 hēkona (tae atu ki te 180°) kia puta rā anō he totoka mārō. Ka whakatauhia te BCT o te tauira mā te maha o ngā hurihanga o ngā ipu17,18. I runga i te BCT, e rua ngā hanganga tino pai mai i te NFRCS Cm, Ch me Cp i tīpakohia mō ngā rangahau whakaahuatanga anō.
I whakatauhia te BCT o ngā hanganga Ch NFRCS me Cp NFRCS mā te whakamahi i te tikanga i whakaahuahia e Li et al. 19. Whakatakotoria he 15 x 15 mm2 Ch NFRCS, Cp NFRCS, me Cs (mana whakahaere pai) ki roto i ngā rihi Petri motuhake (37 °C). I konatunatua te toto kei roto ko te 3.8% konutai citrate ki te 0.2 M CaCl2 i roto i te ōwehenga rōrahi 10:1 hei tīmata i te tukanga toto toto. I pania he 20 µl o te ranunga toto kiore 0.2 M CaCl2 ki te mata o te tauira, ā, i whakanohoia ki roto i tētahi rihi Petri kau. Ko te mana whakahaere ko te toto i ringihia ki roto i ngā rihi Petri kau, kāore he Ct. I ngā wā kua whakaritea o te 0, 3, me te 5 meneti, kati te toto toto mā te tāpiri i te 10 ml o te wai whakakore iona (DI) ki te tauira kei roto te rihi me te kore e whakararuraru i te toto totoka. Ka hemolysis ngā erythrocytes kāore i te totoka (erythrocytes) i te aroaro o te wai whakakore iona, ā, ka tukuna te hemoglobin. I inehia te toto toto i ngā wā rerekē (HA(t)) i te 540 nm (te toto toto λmax) mā te whakamahi i te ine UV-Vis. Ko te mimititanga tino o te toto toto (AH(0)) i roto i te 0 meneti o te 20 µl o te toto i roto i te 10 ml o te wai whakakore iona i tangohia hei paerewa tohutoro. I tatauhia te mimititanga toto toto toto (RHA) o te toto totoka mai i te ōwehenga HA(t)/HA(0) mā te whakamahi i te puranga toto kotahi.
Mā te whakamahi i tētahi tātari kakano (Texture Pro CT V1.3 Build 15, Brookfield, USA), i whakatauhia ngā āhuatanga piri o te NFRK ki ngā kiko kua pakaru. Pēhia he rihi porotaka tuwhera ki te taha o roto o te kiri poaka (me te kore he paparanga ngako). I whakamahia ngā tauira (Ch NFRCS me Cp NFRCS) mā te cannula ki roto i ngā pokepoke porotaka hei hanga piri ki te kiri o te poaka. I muri i te whakatōnga mō te 3 meneti i te pāmahana rūma (RT) (25° C.), i tuhia te kaha piri o te NFRCS i te tere pumau o te 0.5 mm/hēkona.
Ko te āhuatanga matua o ngā hiri pokanga ko te whakanui ake i te toto totoka me te whakaiti i te ngaronga o te toto. I aromatawaihia te toto totoka kore-ngaro i roto i te NFRCS mā te whakamahi i tētahi tikanga kua whakaputaina i mua me ngā whakarerekētanga iti 19. Hangaia he ngongo microcentrifuge (2 ml) (diameter o roto 10 mm) me te kōhao 8 × 5 mm2 i tētahi taha o te ngongo centrifuge (e tohu ana i tētahi patunga tuwhera). Ka whakamahia te NFRCS hei kati i te whakatuwheratanga, ā, ka whakamahia te rīpene hei hiri i ngā taha o waho. Tāpirihia he 20 µl o te 0.2 M CaCl2 ki te ngongo microcentrifuge kei roto te 3.8% sodium citrate premix. I muri i te 10 meneti, ka tangohia ngā ngongo microcentrifuge mai i ngā rihi, ā, i whakatauhia te pikinga o te papatipu o ngā rihi nā te rere o te toto mai i te NFRK (n = 3). I whakaritea te ngaronga toto Ch NFRCS me Cp NFRCS ki te Cs.
I whakatauhia te kounga mākū o te NFRCS i runga i te tikanga i whakaahuahia e Mishra rāua ko Chaudhary21 me ngā whakarerekētanga iti. Whakanohoia te NFRCS ki roto i tētahi pounamu Erlenmeyer 100 ml me te 50 ml wai ka hurihuri mō te 60 hēkona me te kore e hanga he taupoki. Tirohia ā-kanohi me te whakarite i ngā tauira mō te kounga ā-tinana i runga i te kohinga.
I rangahauhia te kaha here o te HFFC ki te Ct mā te whakamahi i ngā tikanga kua whakaputaina i mua me ngā whakarerekētanga iti. I aromatawaihia te kounga o te paninga mata mā te whakaatu i te NFRK ki ngā ngaru oro (whakaohooho o waho) i te aroaro o te wai milliQ (Ct). Ko ngā NFRCS Ch NFRCS me Cp NFRCS kua whakawhanakehia i whakanohoia ki roto i tētahi ipu kua whakakiia ki te wai, ā, i whakamātauria mō te 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, me te 30 meneti. I muri i te maroke, i whakamahia te ōrau rerekētanga i waenga i te taumaha tīmatanga me te taumaha whakamutunga o te NFRCS hei tatau i te ōrau ngaronga o te rauemi (HFFC). I tautokohia anō e te BCT in vitro te kaha here, te ngaronga rānei o ngā rauemi mata. Ko te whai huatanga o te here o te HFFC ki te Ct ka whakarato i te toto totoka me te paninga pīwariwari i runga i te mata o te Ct22.
I whakatauhia te ōritetanga o te NFRCS kua whakawhanakehia e te BCT o ngā tauira (30 mg) i tangohia mai i ngā wāhi whānui kua tīpakohia matapōkeretia o te NFRCS. Whāia te tukanga BCT kua whakahuatia i mua hei whakatau i te tutuki o te NFRCS. Mā te tata i waenga i ngā tauira e rima ka whakarite kia ōrite te kapi o te mata me te whakatakotoranga HFFC ki roto i te mata Ct.
I whakatauhia te rohe whakapā toto ingoa (NBCA) e ai ki te pūrongo o mua me ētahi whakarerekētanga. Whakapūmautia te toto mā te pupuri i te 20 µl o te toto i waenganui i ngā mata e rua o Ct, Ch NFRCS, Cp NFRCS me Cs. I muri i te 1 hāora, i wehea ngā wāhanga e rua o te stent, ā, i ine ā-ringa i te rohe o te toto totoka. Ko te uara toharite o ngā tāruatanga e toru i kiia ko NBCA NFRCS19.
I whakamahia te tātaritanga Dynamic Vapor Sorption (DVS) hei aromatawai i te whai huatanga o te NFRCS ki te mimiti i te wai mai i te taiao o waho, mai i te wāhi whara rānei e whai kawenga ana mō te tīmatanga o te totohu. Ka aromatawai, ka tuhi rānei te DVS i te mimiti me te ngaronga o te kohu i roto i te tauira mā te whakamahi i te taurite tino tairongo me te taumira papatipu o ±0.1 µg. Ka whakaputahia he pēhanga kohu wāhanga (haumākū whanaunga) e te kaiwhakahaere rere papatipu hiko huri noa i te tauira mā te whakaranu i ngā hau kawe kukū me ngā hau kawe maroke. E ai ki ngā aratohu a te European Pharmacopeia, i runga i te ōrau o te mimiti o te makuku e ngā tauira, i whakarōpūtia ngā tauira ki ngā wāhanga e 4 (0–0.012% w/w− kore-waikawa, 0.2–2% w/w paku waikawa, 2–15% āhua waikawa, me te > 15% tino waikawa)23. E ai ki ngā aratohu a te European Pharmacopeia, i runga i te ōrau o te mimiti o te makuku e ngā tauira, i whakarōpūtia ngā tauira ki ngā wāhanga e 4 (0–0.012% w/w− kore-waikawa, 0.2–2% w/w paku waikawa, 2–15% āhua waikawa, me te > 15% tino waikawa)23.E ai ki ngā taunakitanga a te European Pharmacopoeia, i runga i te ōrau o te mimiti makuku e ngā tauira, i wehea ngā tauira kia 4 ngā wāhanga (0–0.012% w/w – kāore e waikura, 0.2–2% w/w paku waikura, 2–15%).% умеренно гигроскопичен и > 15% очень гигроскопичен)23. % āhua waikura, ā, > 15% tino waikura)23.根据欧洲药典指南，根据样品吸收水分的百分比，样品分为4 类（0-0.012% w/w-2% w/w- 湁們、 w/w 轻微吸湿性、2-15 % 适度吸湿，> 15% 非常吸湿）23.根据 欧洲 药典 指南 ， 根据 吸收 水分 的 百分比 样品 分为 分为 分为 分为 分为 分为 类 2（2（2（2（吸湿 性 、 、 、 、 0.2-2% W/w 轻微 、 2-15% 适度 吸湿 ，> 15 %非常吸湿）23.E ai ki ngā taunakitanga a te European Pharmacopoeia, ka wehea ngā tauira kia 4 ngā akomanga i runga i te ōrau o te makuku e mimitihia ana e te tauira (0-0.012% te taumaha – kāore e waikura, 0.2-2% te taumaha he waikura paku, 2-15% te taumaha).% умеренно гигроскопичен, > 15 % очень гигроскопичен) 23. % āhua waikura, > 15% tino waikura) 23.I whakatauhia te whai huatanga hygroscopic o te NFCS X NFCS me te TsN NFCS i runga i te tātaritanga DVS TA TGA Q5000 SA. I roto i tēnei tukanga, i whiwhihia te wā whakahaere, te haumākū whanaunga (RH), me te taumaha tauira wā-tūturu i te 25°C24. Ka tatauhia te ihirangi makuku mā te tātaritanga papatipu NFRCS tika mā te whakamahi i te whārite e whai ake nei:
Ko te MC te haumākū o te NFRCS. m1 – te taumaha maroke o ngā NSAID. Ko te m2 te papatipu NFRCS wā-tūturu i tētahi RH kua hoatu.
I whakatauhia te horahanga mata katoa mā te whakamahi i tētahi whakamātautau mimiti hauota me te hauota wai i muri i te whakawāteatanga o ngā tauira i te 25 °C mō ngā hāora 10 (< 7 × 10–3 Torr). I whakatauhia te horahanga mata katoa mā te whakamahi i tētahi whakamātautau mimiti hauota me te hauota wai i muri i te whakawāteatanga o ngā tauira i te 25 °C mō ngā hāora 10 (< 7 × 10–3 Torr). Общая площадь поверхности оценивалась с помощью эксперимента по адсорбции азота жидким азотом после опорожнраце опорожнец течение 10 ч (< 7 × 10–3 Торр). I whakatauhia te horahanga katoa o te mata mā te whakamahi i tētahi whakamātautau mimiti hauota me te hauota wai i muri i te whakawāteatanga o ngā tauira i te 25°C mō ngā hāora 10 (< 7 × 10–3 Torr).在25°C 清空样品10 小时（< 7 × 10-3 Torr）后，使用液氮的氮吸附实验估计总表面积。在 25°C Общая площадь поверхности оценивалась с использованием экспериментов по адсорбции азота жидким азотом порослеоц течение 10 часов при 25°C (< 7 × 10-3 торр). I whakatauhia te horahanga katoa o te mata mā te whakamahi i ngā whakamātautau mimiti hauota me te hauota wai i muri i te whakawāteatanga o ngā tauira mō ngā hāora 10 i te 25°C (< 7 x 10-3 torr).I whakatauhia te horahanga mata katoa, te rōrahi o ngā pore me te rahi o ngā pore NFRCS ki te Quantachrome mai i NOVA 1000e, Austria mā te whakamahi i te pūmanawa RS 232.
Whakaritea he 5% ngā toto whero (he tote hei waimeha) mai i te toto katoa. Kātahi ka whakawhitia he wāhanga o te HFFC (0.25 ml) ki tētahi pereti 96-pūnga me te papatipu toto whero 5% (0.1 ml). Whakatōkia te ranunga ki te 37°C mō te 40 meneti. I whakaarohia he ranunga o ngā toto whero me te serum hei mana whakahaere pai, ā, ko te ranunga o ngā toto tote me ngā toto whero hei mana whakahaere kino. I whakatauhia te Hemagglutination e ai ki te tauine Stajitzky. Ko ngā tauine kua whakaarohia koia ēnei: ​​+ + + + ngā kohikohinga kirikiri matotoru; + + + ngā papa raro maeneene me ngā taha piko; + + ngā papa raro maeneene me ngā taha haehae; + ngā mowhiti whero whaiti huri noa i ngā taha o ngā papa maeneene; – (kino) pātene whero motuhake 12 i waenganui o te puna o raro.
I rangahauhia te hototahitanga toto o te NFRCS i runga anō i te tikanga a te International Organization for Standardization (ISO) (ISO10993-4, 1999)26,27. Ko te tikanga ine taumaha i whakaahuatia e Singh et al. I mahia he whakarerekētanga iti hei aromatawai i te hanganga thrombus i te aroaro o te NFRCS, i runga rānei i te mata o te NFRCS. I whaowhia te 500 mg o te Cs, Ch NFRCS me te Cp NFRCS ki roto i te tote phosphate buffered (PBS) mō ngā hāora 24 i te 37°C. I muri i ngā hāora 24, i tangohia te PBS, ā, i rongoatia te NFRCS ki te 2 ml o te toto kei roto ko te 3.8% sodium citrate. I runga i te mata o te NFRCS, tāpirihia te 0.04 ml o te 0.1 M CaCl2 ki ngā tauira i whaowhia. I muri i ngā meneti 45, i tāpirihia te 5 ml o te wai whakakore hei aukati i te toto totoka. I rongoatia te toto totoka i runga i te mata o te NFRK ki te otinga formaldehyde 36-38%. I whakamaroketia, ā, i paunatia ngā toto totoka i whakapūmautia ki te formaldehyde. I whakatauhia te ōrau o te toto totohu mā te tatau i te taumaha o te karāhe kāore he toto me te tauira (mana whakahaere kino) me te karāhe kei roto he toto (mana whakahaere pai).
Hei whakaū tuatahi, i tirohia ngā tauira i raro i te karuārai whatu kia mārama ai ki te kaha o te paninga mata HFFC, te hononga Ct, me te whatunga Ct ki te hanga pore. I tapahia ngā wāhanga angiangi o Ch me Cp mai i te NFRCS ki te mata o te maripi koi. I whakatakotoria te wāhanga hua ki runga i te kiriata karāhe, i hipokina ki te uhi, ā, i whakapirihia ngā taha ki te kāpia. I tirohia ngā kiriata kua whakaritea i raro i te karuārai whatu, ā, i tangohia ngā whakaahua i ngā whakanuinga rerekē.
I tirohia te whakatakotoranga polima i roto i ngā whatunga Ct mā te whakamahi i te karuārai fluorescence i runga i te tikanga i whakaahuahia e Rice et al.29. I konatunatua te hanganga HFFC i whakamahia mō te whakatakotoranga ki tētahi tae hihiko (amaranth), ā, i whakaritea ngā NFRCS (Ch me Cp) e ai ki te tikanga i whakahuatia i mua. I konatunatua te hanganga HFFC i whakamahia mō te whakatakotoranga ki tētahi tae hihiko (amaranth), ā, i whakaritea ngā NFRCS (Ch me Cp) e ai ki te tikanga i whakahuatia i mua.I konatunatua te hanganga HFFC i whakamahia mō te hanganga ki tētahi tae hihiko (amaranth) ā, i whiwhihia te NFRCS (Ch me te Cp) e ai ki te tikanga i whakahuatia i mua.HFFCHFFCI konatunatua te hanganga HFFC i whakamahia i roto i te whakatakotoranga ki tētahi tae hihiko (Amaranth) ā, i whiwhi i te NFRCS (Ch me te Cp), e ai ki te kōrero i mua ake nei.I tapahia ngā wāhanga angiangi o te NFRK mai i ngā tauira i whiwhihia, i whakatakotoria ki runga i ngā kiriata karāhe, ā, i hipokina ki ngā kiriata uhi. Mātakihia ngā kiriata kua whakaritea i raro i te karuārai hihi-ā-rangi mā te whakamahi i te tātari matomato (310-380 nm). I tangohia ngā whakaahua i te whakanui 4x hei mārama ki ngā whanaungatanga Ct me te whakatakotoranga polymer nui rawa atu i roto i te whatunga Ct.
I whakatauhia te āhua o te mata o te NFRCS Ch me te Cp mā te whakamahi i tētahi karuārai kaha ngota (AFM) me tētahi cantilever TESP tino koi i te aratau pato: 42 N/m, 320 kHz, ROC 2-5 nm, Bruker, Taiwan. I whakatauhia te taratara o te mata mā te tapawhā toharite pūtake (RMS) mā te whakamahi i te pūmanawa (Scanning Probe Image Proceed). I whakaaturia ngā wāhi NFRCS maha ki ngā whakaahua 3D hei tirotiro i te ōritetanga o te mata. Ko te paerewa rerekētanga o te kaute mō tētahi wāhi kua hoatu ka tautuhia ko te taratara o te mata. I whakamahia te whārite RMS hei ine i te taratara o te mata o te NFRCS31.
I whakahaerehia ngā rangahau e hangai ana ki te FESEM mā te whakamahi i te FESEM, SU8000, HI-0876-0003, Hitachi, Tokyo, hei mārama ki te āhua o te mata o te Ch NFRCS me te Cp NFRCS, i whakaatu i te BCT pai ake i te Cm NFRCS. I whakahaerehia te rangahau FESEM i runga i te tikanga i whakaahuahia e Zhao et al. 32 me ngā whakarerekētanga iti. I konatunatua ngā NFRCS 20 ki te 30 mg Ch NFRCS me te Cp NFRCS ki te 20 µl o te 3.8% konutai citrate i konatunatua ki te toto kiore. I tāpirihia te 20 μl o te 0.2 M CaCl2 ki ngā tauira i rongoatia ki te toto hei tīmata i te toto totoka, ā, i whakatōkia ngā tauira i te pāmahana rūma mō te 10 meneti. Hei tāpiri, i tangohia ngā erythrocytes taikaha mai i te mata o te NFRCS mā te horoi ki te tote.
I rongoatia ngā tauira i muri mai ki te 0.1% glutaraldehyde, kātahi ka whakamaroketia ki roto i te oumu hau wera i te 37°C hei tango i te makuku. I pania, ā, i tātarihia ngā tauira maroke 32. Ko ētahi atu whakaahua i riro mai i te tātari ko te hanganga totoka i runga i te mata o ngā muka miro takitahi, te whakatakotoranga polymer i waenga i te Ct, te āhua o ngā erythrocyte (āhua), te pumau o te totoka, me te āhua o ngā erythrocyte i te aroaro o te NFRCS. Ko ngā wāhi NFRCS kāore i rongoatia me ngā wāhi NFRCS i rongoatia ki te Ch me te Cp i whakatōkia ki te toto i matawaihia mō ngā katote huānga (konutai, pāhare pāporo, hauota, konupūmā, konupora, konutea, parahi me te selenium)33. Whakatairitehia ngā ōrau katote huānga i waenga i ngā tauira i rongoatia me ngā tauira kāore i rongoatia kia mārama ai ki te kohikohinga katote huānga i te wā o te hanganga totoka me te ōritetanga o te totoka.
I whakatauhia te matotoru o te paninga mata Cp HFFC i runga i te mata Ct mā te whakamahi i te FESEM. I tapahia ngā wāhanga whakawhiti o te Cp NFRCS mai i te anga, ā, i pania ki te sputter. I tirohia ngā tauira paninga sputter i puta mai mā te FESEM, ā, i inehia te matotoru o te paninga mata 34, 35, 36.
Mā te hihi-X micro-CT ka whakaratohia he whakaahua 3D teitei-taumira kore-whakangaro, ā, ka taea e koe te ako i te whakaritenga hanganga o roto o te NFRK. Ka whakamahia e te Micro-CT he hihi-X e haere ana i roto i te tauira hei tuhi i te tauwehenga whakaiti raina ā-rohe o ngā hihi-X i roto i te tauira, e āwhina ana ki te tiki mōhiohio āhua. I tirotirohia te taunga o roto o te Ct i roto i te Cp NFRCS me te Cp NFRCS i rongoatia ki te toto mā te micro-CT hei mārama ki te whai huatanga o te mimiti me te toto totoka i te aroaro o te NFRCS37,38,39. I hangaia anō ngā hanganga 3D o ngā tauira Cp NFRCS i rongoatia ki te toto me te kore i rongoatia mā te whakamahi i te micro-CT (V|tome|x S240, Phoenix, Tiamana). Mā te whakamahi i te pūmanawa VG STUDIO-MAX putanga 2.2, he maha ngā whakaahua hihi-X i tangohia mai i ngā koki rerekē (ko te mea pai ko te kapinga 360°) hei whakawhanake i ngā whakaahua 3D mō te NFRCS. I hangaia anō ngā raraunga whakaata i kohia hei whakaahua rōrahi 3D mā te whakamahi i te pūmanawa 3D ScanIP Academic ngāwari e rite ana.
Hei tāpiritanga, kia mārama ai ki te tohatoha o te toto totoka, i tāpirihia he 20 µl o te toto kua whakaranua ki te citrate me te 20 µl o te 0.2 M CaCl2 ki te NFRCS hei tīmata i te toto totoka. Ka waiho kia mārō ngā tauira kua whakaritea. I rongoatia te mata o te NFRK ki te 0.5% glutaraldehyde, ā, i whakamaroketia ki roto i te oumu hau wera i te 30–40°C mō te 30 meneti. I matawaihia te toto totoka i hangaia ki runga i te NFRCS, i hangaia anō, ā, i whakaaturia he whakaahua 3D o te toto totoka.
I whakahaerehia ngā whakamātautau paturopi ki runga i te Cp NFRCS (he pai rawa atu ki te Ch NFRCS) mā te whakamahi i te tikanga kua whakaahuatia i mua me ngā whakarerekētanga iti. I whakatauhia te kaha paturopi o te Cp NFRCS me te Cp HFFC mā te whakamahi i ngā moroiti whakamātautau rerekē e toru [S.aureus (huakita karamu-pai), E.coli (huakita karamu-kino) me te Candida mā (C.albicans)] e tipu ana i runga i te agar i roto i ngā rihi Petri i roto i te incubator. Whakatōkia kia ōrite te 50 ml o te whakatārewatanga ahurea huakita kua whakaranua ki te kukū o te 105-106 CFU ml-1 ki runga i te reo agar. Ringihia te reo ki roto i te rihi Petri ka waiho kia totoka. I hangaia he poka ki runga i te mata o te pereti agar hei whakakī ki te HFFC (3 ngā poka mō te HFFC me te 1 mō te mana kino). Tāpirihia te 200 µl HFFC ki ngā poka e 3 me te 200 µl pH 7.4 PBS ki te poka tuawhā. I tērā taha o te rihi petri, whakatakotoria he kōpae Cp NFRCS 12 mm ki runga i te agar-agar kua totoka, ka whakamakuku ki te PBS (pH 7.4). Ko ngā papa Ciprofloxacin, ampicillin me te fluconazole e kiia ana he paerewa tohutoro mō Staphylococcus aureus, Escherichia coli me Candida albicans. Inehia ā-ringa te rohe aukati, ka tango whakaahua mamati o te rohe aukati.
I muri i te whakaaetanga matatika a te whare wānanga, i whakahaerehia te rangahau i te Kasturba Medical College of Education and Research i Manipal, Karnataka, i te tonga o Īnia. Kua arotakehia, ā, kua whakaaetia te kawa whakamātautau TEG in vitro e te Komiti Matatika Whare Wānanga o te Kasturba Medical College, Manipal, Karnataka (IEC: 674/2020). I tohua ngā kaupapa mai i ngā kaituku toto tūao (18 ki te 55 tau te pakeke) mai i te peeke toto o te hōhipera. Hei tāpiri, i riro mai he puka whakaaetanga whai whakaaro mai i ngā tūao mō te kohikohi tauira toto. I whakamahia te TEG taketake (N-TEG) ​​hei ako i te pānga o te hanganga Cp HFFC ki te toto katoa kua konatunatua ki te konutai citrate. E mōhiotia whānuitia ana te N-TEG mō tana mahi i roto i te whakaora i te wā tiaki, e hanga raruraru ana mō ngā tākuta nā te mea ka roa pea ngā hua (ngā whakamātautau toto toto auau). I whakahaerehia te tātaritanga N-TEG mā te whakamahi i te toto katoa. I riro mai te whakaaetanga whai whakaaro me te hītori hauora taipitopito mai i ngā kaiuru katoa. Kāore i whakaurua ki te rangahau ngā kaiuru me ngā raruraru hemostatic, thrombotic rānei pērā i te haputanga/muri i te whānautanga, te mate ate rānei. I tangohia atu hoki ngā tāngata e tango ana i ngā rongoā e pā ana ki te rerenga toto toto i roto i te rangahau. I whakahaerehia ngā whakamātautau taiwhanga taketake (hemoglobin, prothrombin time, activated thromboplastin me te tatau pereti toto) ki ngā kaiuru katoa e ai ki ngā tikanga paerewa. Ka whakatauhia e te N-TEG te viscoelasticity o te toto toto, te hanganga toto toto tuatahi, te taunekeneke matūriki, te whakapakari toto toto toto, me te pakaru toto toto toto. Ka whakaratohia e te tātaritanga N-TEG ngā raraunga whakairoiro me ngā raraunga tau mō ngā pānga whakakotahi o ētahi huānga pūtau me te plasma. I whakahaerehia te tātaritanga N-TEG ki runga i ngā rahinga rerekē e rua o te Cp HFFC (10 µl me te 50 µl). Nō reira, i tāpirihia te 1 ml o te toto katoa me te waikawa citric ki te 10 μl o te Cp HFFC. Tāpirihia te 1 ml (Cp HFFC + toto citrated), 340 µl toto whakauru ki te 20 µl 0.2 M CaCl2 kei roto te rihi TEG. I muri iho, ka utaina ngā rihi TEG ki roto i te TEG® 5000, US hei ine i te R, K, te koki alpha, MA, G, CI, TPI, EPL, LY 30% o ngā tauira toto i te aroaro o te Cp HFFC41.
I arotakehia, ā, i whakaaetia hoki te kawa rangahau in vivo e te Komiti Matatika Kararehe Whare (IAEC), Kura Rongoā Kasturba, Whare Wānanga Mātauranga Teitei o Manipal, Manipal (IAEC/KMC/69/2020). I whakahaerehia ngā whakamātautau kararehe katoa i runga anō i ngā taunakitanga a te Komiti mō te Whakahaere me te Tirotiro i ngā Whakamātautau Kararehe (CPCSEA). I ​​whakahaerehia ngā rangahau NFRCS in vivo katoa (2 × 2 cm2) ki ngā kiore Wistar uha (200 ki te 250 karamu te taumaha). I whakangāwarihia ngā kararehe katoa ki te pāmahana 24-26°C, ā, i wātea te urunga atu o ngā kararehe ki te kai me te wai paerewa ad libitum. I wehea matapōkeretia ngā kararehe katoa ki ngā rōpū rerekē, e toru ngā kararehe i roto i ia rōpū. I whakahaerehia ngā rangahau katoa i runga anō i te Rangahau Kararehe: Pūrongo o ngā Whakamātautau In Vivo 43. I mua i te rangahau, i whakamoea ngā kararehe mā te werohanga ā-roto (ip) o te ranunga o te 20-50 mg o te ketamine (mō ia 1 kg o te taumaha tinana) me te 2-10 mg o te xylazine (mō ia 1 kg o te taumaha tinana). I muri i te rangahau, i tatauhia te nui o te toto mā te aromatawai i te rerekētanga i waenga i te taumaha tīmatanga me te taumaha whakamutunga o ngā tauira, ā, ko te uara toharite i whiwhi mai i ngā whakamātautau e toru i tangohia hei nui o te toto o te tauira.
I whakatinanahia te tauira tapahi hiku kiore hei mārama ki te kaha o te NFRCS ki te whakahaere i te toto i roto i te whara, te pakanga, te aituā waka rānei (tauira whara). Tapahia te 50% o te hiku ki te mata o te maripi ka waiho ki te hau mō te 15 hēkona hei whakarite i te toto noa. Hei tāpiri, i whakanohoia ngā tauira whakamātautau ki runga i te hiku o te kiore mā te pēhi (Ct, Cs, Ch NFRCS me Cp NFRCS). I pūrongohia te toto me te PCT mō ngā tauira whakamātautau (n ​​= 3)17,45.
I tirotirohia te whai huatanga o te whakahaere pēhanga NFRCS i roto i te pakanga i runga i tētahi tauira o te uaua femoral o runga. Ka whakaatuhia te uaua femoral, ka werohia ki te trocar 24G, ā, ka whakahekehia te toto i roto i te 15 hēkona. I muri i te kitenga o te toto kāore i te whakahaeretia, ka whakatakotoria te tauira whakamātautau ki te wāhi werohia me te pēhanga. I muri tonu i te whakamahinga o te tauira whakamātautau, ka tuhia te wā totoka, ā, ka kitea te whai huatanga o te toto mō ngā meneti e 5 e whai ake nei. I whakahokia te tukanga kotahi ki a Cs me Ct46.
I whakaarohia e Dowling et al. 47 tētahi tauira whara ate hei aromatawai i te pūmanawa hemostatic o ngā rauemi hemostatic i roto i te horopaki o te toto i te wā o te pokanga. I tuhia te BCT mō ngā tauira Ct (mana whakahaere kino), te anga Cs (mana whakahaere pai), ngā tauira Ch NFRCS, me ngā tauira Cp NFRCS. I whakaatuhia te vena cava suprahepatic o te kiore mā te mahi i te laparotomy waenga. Whai muri i tēnā, i tapahia te wāhanga tawhiti o te lobe maui ki te kutikuti. Hangaia he tapahi ki te ate ki te mata o te maripi koi, ā, tukua kia toto mō ētahi hēkona. I paunatia tika ngā tauira whakamātautau Ch NFRCS me Cp NFRCS ki runga i te mata kua pakaru me te kore he pēhanga pai, ā, i tuhia te BCT. Kātahi ka pēhia e te rōpū whakahaere (Ct) ā, i muri mai ko te Cs 30 s47 me te kore e whati te whara.
I whakahaerehia ngā whakamātautau whakaora patunga in vivo mā te whakamahi i tētahi tauira patunga tapahi hei aromatawai i ngā āhuatanga whakaora patunga o ngā NFRCS i ahu mai i te polymer kua whakawhanakehia. I tīpakohia ngā tauira o ngā patunga tapahi, ā, i whakahaerehia i runga anō i ngā tikanga kua whakaputaina i mua me ngā whakarerekētanga iti19,32,48. I whakamoea ngā kararehe katoa e ai ki te whakaahuatanga o mua. Whakamahia he poka koiora (12 mm) hei hanga i tētahi tapahi porohita hohonu ki te kiri o te tuara. I whakakakahuhia ngā wāhi patunga kua whakaritea ki te Cs (mana whakahaere pai), Ct (me te mōhio ka pāngia te whakaora e ngā papa miro), Ch NFRCS me Cp NFRCS (rōpū whakamātautau) me tētahi mana whakahaere kino me te kore he maimoatanga. I ia rā o te rangahau, i inehia te horahanga o te patunga i roto i ngā kiore katoa. Whakamahia he kāmera mamati hei tango pikitia o te wāhi patunga, ka whakakakahu i tētahi takai hou. I inehia te ōrau o te katinga patunga e te tātai e whai ake nei:
I runga i te ōrau o te katinga o te patunga i te 12 o ngā rā o te rangahau, i tangohia te kiri kiore o te rōpū pai rawa atu ((Cp NFRCS) me te rōpū whakahaere) ā, i rangahaua mā te tae H&E me te tae trichrome a Masson. I runga i te ōrau o te katinga o te patunga i te 12 o ngā rā o te rangahau, i tangohia te kiri kiore o te rōpū pai rawa atu ((Cp NFRCS) me te rōpū whakahaere) ā, i rangahaua mā te tae H&E me te tae trichrome a Masson.I runga i te ōrau o te katinga o te patunga i te 12 o ngā rā o te rangahau, i tangohia te kiri o ngā kiore o te rōpū pai rawa atu ((Cp NFRCS) me te rōpū whakahaere) ā, i tirohia mā te whakakoi ki te hematoxylin-eosin me te Masson's trichrome.根据研究第12天的伤口闭合百分比，切除最佳组((Cp NFRCS)和对照组)的大鼠皮肤，进行H&E染色和Masson三色染色研究。根据研究第12天的伤口闭合百分比，切除最佳组((Cp NFRCS)和对照组)的大鼠皮肤，进行H&E染色和眳组）I tangohia ngā kiore i roto i te rōpū pai rawa atu ((Cp NFRCS) me ngā rōpū mana whakahaere) mō te tae hematoxylin-eosin me te tae trichrome a Masson i runga i te ōrau o te katinga o te patunga i te rā 12 o te rangahau.I whakahaerehia te tukanga whakatā i whakatinanahia i runga anō i ngā tikanga kua whakamāramahia i mua49,50. I muri i te whakapūmautanga ki te 10% formalin, i whakamaroketia ngā tauira mā te whakamahi i tētahi raupapa o ngā waipiro kua whakarōpūtia. Whakamahia he microtome hei tiki i ngā wāhanga angiangi (5 µm te matotoru) o te kiko kua tangohia. I rongoatia ngā wāhanga raupapa angiangi o ngā mana whakahaere me te Cp NFRCS ki te hematoxylin me te eosin hei ako i ngā huringa histopathological. I whakamahia te whakatā trichrome a Masson hei kite i te hanganga o ngā fibril collagen. Ko ngā hua i puta i akohia matapotia e ngā tohunga pathology.
I tirotirohia te pumau o ngā tauira Cp NFRCS i te pāmahana rūma (25°C ± 2°C/60% RH ± 5%) mō ngā marama 1251. I tirotirohia ā-kanohitia te Cp NFRCS (te whakarerekētanga o te mata me te tipu moroiti) me te whakamātautau mō te ātete ki te kakahu takai me te BCT e ai ki ngā tikanga i runga ake nei kua whakarārangihia i roto i te wāhanga Rauemi me ngā Tikanga.
I tirotirohia te āheinga tauine me te āheinga tāruarua o te Cp NFRCS mā te whakarite i te Cp NFRCS me te rahi o te 15 × 15 cm2. Hei tāpiri, i tangohia ngā tauira 30 mg (n = 5) mai i ngā wāhanga Cp NFRCS rerekē, ā, i aromatawaitia te BCT o ngā tauira i akohia e ai ki te whakaahuatanga o mua i te wāhanga Tikanga.
Kua ngana mātou ki te whakawhanake i ngā āhua me ngā hanganga rerekē mā te whakamahi i ngā hanganga Cp NFRCS mō ngā tono hauora rerekē. Kei roto i ēnei āhua, i ēnei whirihoranga rānei, ngā ūkui kōniko mō te toto ihu, ngā mahi niho, me ngā ūkui porotaka mō te toto tara.
Ko ngā huinga raraunga katoa e whakaatuhia ana hei toharite ± paerewa rerekētanga, ā, i tātarihia mā te ANOVA mā te whakamahi i te Prism 5.03 (GraphPad, San Diego, CA, USA) ā, i whai ake ko te whakamātautau whakatairite maha a Bonferroni (*p<0.05).
I rite ngā tikanga katoa i mahia i roto i ngā rangahau tangata ki ngā paerewa o te Institute me te National Research Council, tae atu ki te Whakapuakitanga o Helsinki 1964 me ōna whakatikatika i muri mai, me ngā paerewa matatika rite rānei. I whakamōhiotia ngā kaiuru katoa mō ngā āhuatanga o te rangahau me tōna āhua tūao. Ka noho muna ngā raraunga o te kaiuru ina kohia. Kua arotakehia, ā, kua whakaaetia te kawa whakamātautau TEG in vitro e te Komiti Matatika Whare o te Kasturba Medical College, Manipal, Karnataka (IEC: 674/2020). I hainatia e ngā tūao te whakaaetanga whai whakaaro ki te kohi tauira toto.
I whakahaerehia ngā tikanga katoa i mahia i roto i ngā rangahau kararehe i runga anō i ngā aratohu a te Kastuba Faculty of Medicine, Manipal Institute of Higher Education, Manipal (IAEC/KMC/69/2020). I whakahaerehia ngā whakamātautau kararehe katoa i runga anō i ngā aratohu a te Komiti mō te Whakahaere me te Tirotiro i ngā Whakamātautau Kararehe (CPCSEA). E whai ana ngā kaituhi katoa i ngā aratohu a ARRIVE.
I tātarihia ngā irahiko FTIR o ngā NFRCS katoa, ā, i whakaritea ki te irahiko chitosan e whakaaturia ana i te Pikitia 2A. Ngā tihi irahiko āhuatanga o te chitosan (kua tuhia) i te 3437 cm-1 (toto OH me NH, taupatupatu), 2945 me te 2897 cm-1 (toto CH), 1660 cm-1 (momo NH2), 1589 cm-1 (piko N–H), 1157 cm-1 (toto piriti O-), 1067 cm-1 (toto C–O, hydroxyl tuarua), 993 cm-1 (toto CO, Bo-OH) 52.53.54. E whakaatu ana te Ripanga Tāpiri S1 i ngā uara irahiko mimiti FTIR NFRCS mō te chitosan (kaitātari), te chitosan parakore, Cm, Ch, me te Cp. I whakaatuhia e ngā irahiko FTIR o ngā NFRCS katoa (Cm, Ch me te Cp) ngā roopu mimiti āhuatanga ōrite ki te chitosan parakore, kāore he huringa nui (Pikitia 2A). I whakaū ngā hua FTIR i te korenga o ngā taunekeneke matū, ā-tinana rānei i waenga i ngā pūmua i whakamahia hei whakawhanake i te NFRCS, e tohu ana he koretake ngā pūmua i whakamahia.
Te whakaahuatanga in vitro o Cm NFRCS, Ch NFRCS, Cp NFRCS me Cs. (A) e whakaatu ana i ngā hihi FTIR whakakotahi o ngā hanganga o te chitosan me te Cm NFRCS, Ch NFRCS me te Cp NFRCS i raro i te pēhanga. (B) a) Te tere mimiti toto katoa o te NFRCS Cm, Ch, Cp, me te Cg (n = 3); I whakaatuhia e ngā tauira Ct he BAR teitei ake nā te mea he nui ake te whai huatanga mimiti o te miro miro; b) Te toto i muri i te mimiti toto Whakaahua o te tauira i mimitihia. Whakaaturanga whakairoiro o te BCT o te tauira whakamātautau C (ko te Cp NFRCS te BCT pai rawa atu (15 s, n = 3)). I whakaaturia ngā raraunga i roto i te C, D, E, me te G hei toharite ± SD, ā, ko ngā pae hapa e tohu ana i te SD, ***p < 0.0001. I whakaaturia ngā raraunga i roto i te C, D, E, me te G hei toharite ± SD, ā, ko ngā pae hapa e tohu ana i te SD, ***p < 0.0001. Данные в C, D, E и G представлены как среднее ± стандартное отклонение, а планки погрешностей представляют сталнот, *** <0,0001. Ka whakaatuhia ngā raraunga i roto i te C, D, E, me te G hei toharite ± paerewa rerekētanga, ā, ko ngā pae hapa e tohu ana i te paerewa rerekētanga, ***p<0.0001. C、D、E 和G 中的数据显示为平均值± SD，误差线代表SD，***p <0.0001。 C、D、E 和G 中的数据显示为平均值± SD，误差线代表SD，***p <0.0001。 Данные в C, D, E и G показаны как среднее значение ± стандартное отклонение, планки погрешностей представляют сталнот, *** <0,0001. Ko ngā raraunga i roto i te C, D, E, me te G e whakaaturia ana hei toharite ± paerewa rerekētanga, ko ngā pae hapa e tohu ana i te paerewa rerekētanga, ***p<0.0001.