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Ọbara ọbara na-enweghị njikwa bụ otu n'ime ihe ndị kacha akpata ọnwụ. Ime ka ọbara na-agba ọsọ ngwa ngwa na-eme ka onye ahụ dị ndụ dị ka enyemaka mbụ n'oge agha, ihe mberede okporo ụzọ, na ọrụ mbelata ọnwụ. Ihe owuwu nke eriri na-eme ka eriri na-eme ka ọ sie ike (NFRCS) nke sitere na ihe mejupụtara hemostatic film-forming (HFFC) dị mfe dị ka usoro na-aga n'ihu nwere ike ịkpalite ma melite hemostasis. Mmepe nke NFRCS dabere na nhazi nke nku dragonfly. Nhazi nku dragonfly nwere nku transverse na longitudinal, a na-ejikọkwa akpụkpọ ahụ nku na ibe ha iji nọgide na-eguzosi ike n'ezi ihe nke microstructure. HFFC na-ekpuchi elu eriri ahụ n'otu aka ahụ na ihe nkiri nke nanometer ọkpụrụkpụ ma na-ejikọ ọkpụrụkpụ owu kesara na-enweghị usoro (Ct) (usoro mgbasa ozi) iji mepụta nhazi nanoporous. Njikọta nke usoro na-aga n'ihu na nke gbasaa na-ebelata ọnụ ahịa nke ngwaahịa ahụ ugboro iri ma e jiri ya tụnyere ngwaahịa ndị dị na azụmaahịa. Enwere ike iji NFRCS gbanwere (tampons ma ọ bụ eriri aka) n'ọtụtụ ngwa biomedical. Nnyocha In vivo ekpebiela na Cp NFRCS emepụtara na-akpali ma na-eme ka usoro coagulation dịkwuo mma n'ebe etinyere ya. NFRCS nwere ike ịgbanwe gburugburu ebe obibi ma rụọ ọrụ n'ọkwa sel n'ihi nhazi ya na-enweghị ihe ọ bụla na-eme ka ọnya gwọọ nke ọma na ụdị ọnya mwepụ ahụ.
Ọbara ọbara a na-achịkwaghị achịkwa n'oge agha, n'oge ịwa ahụ na n'oge mberede nwere ike iyi ndụ ndị merụrụ ahụ egwu dị ukwuu1. Ọnọdụ ndị a na-edugakwa n'ịbawanye n'ozuzu nke iguzogide akwara dị n'akụkụ, na-eduga na nkụchi ọbara. A na-ewere usoro kwesịrị ekwesị iji chịkwaa ọbara ọgbụgba n'oge na mgbe ịwa ahụ gasịrị dị ka ihe nwere ike iyi ndụ egwu2,3. Mmebi nke nnukwu arịa ọbara na-eduga na nnukwu mfu ọbara, na-ebute ọnụego ọnwụ nke ≤ 50% na ọgụ na 31% n'oge ịwa ahụ1. Nnukwu mfu ọbara na-eduga na mbelata olu ahụ, nke na-ebelata mmepụta obi. Mmụba na nguzogide akwara dị n'akụkụ na mmebi na-aga n'ihu nke microcirculation na-eduga na hypoxia na akụkụ ahụ na-akwado ndụ. Mkpọchi ọbara nwere ike ime ma ọ bụrụ na ọnọdụ ahụ aga n'ihu na-enweghị ntinye aka dị irè1,4,5. Nsogbu ndị ọzọ gụnyere ọganihu nke hypothermia na metabolic acidosis, yana nsogbu coagulation nke na-egbochi usoro coagulation. Mkpọchi ọbara dị oke njọ na-ejikọta ya na ihe egwu dị elu nke ọnwụ6,7,8. Na ọkwa nke atọ (n'ihu) nkụchi, mmịnye ọbara dị mkpa maka ndụ onye ọrịa n'oge ọrịa na ọnwụ n'oge ịwa ahụ na mgbe ịwa ahụ gasịrị. Iji merie ọnọdụ niile dị n'elu nke na-eyi ndụ egwu, anyị emepụtala ihe owuwu nanoporous fiber-reinforced composite scaffold (NFRCS) nke na-eji obere mkpokọta polymer (0.5%) site na iji ngwakọta nke polima hemostatic na-agbaze mmiri.
Site n'iji eriri eme ka ọ sie ike, enwere ike ịmepụta ngwaahịa ndị na-adịghị ọnụ ala. Eriri ndị a haziri n'usoro na-eyi nhazi nke nku nnụnụ dragonfly, nke eriri kwụ ọtọ na kwụ ọtọ dị n'elu nku na-edozi. Akwara ndị na-agafe agafe na ogologo nke nku na-ekwurịta okwu na akpụkpọ ahụ nku (Foto 1). NFRCS nwere Ct emelitere dị ka usoro ihe eji eme ka ọ dị mma nke nwere ike anụ ahụ na nke igwe (Foto 1). N'ihi ọnụ ahịa na nkà aka, ndị dọkịta na-awa ahụ na-ahọrọ iji ihe nrịbama eriri owu (Ct) n'oge ọrụ na uwe. Ya mere, n'ihi uru dị iche iche o nwere, gụnyere > 90% kristal cellulose (na-enye aka na mmụba nke ọrụ hemostatic), ejiri Ct mee ihe dị ka usoro ọkpụkpụ nke NFRCS9,10. Ya mere, n'ihi uru dị iche iche o nwere, gụnyere > 90% kristal cellulose (na-enye aka na mmụba nke ọrụ hemostatic), ejiri Ct mee ihe dị ka usoro ọkpụkpụ nke NFRCS9,10. Следовательно, учитывая его многочисленные преимущества, в том числе > 90% klaasị (90%) повышении гемостатической активности), Ct использовали в качестве скелетной системы NFRCS9,10. Ya mere, ebe ọ bụ na e nwere ọtụtụ uru dị na ya, gụnyere >90% kristal cellulose (nke na-etinye aka na mmụba nke ọrụ hemostatic), e jiri Ct mee ihe dị ka sistemu ọkpụkpụ NFRCS9,10.因此，考虑到它的多重益处，包括> 90%的骨架系统。因此，考虑到它的多重益处，包括> 90%Ya mere, ebe ọ bụ na e nwere ọtụtụ uru dị na ya, gụnyere ihe karịrị 90% crystalline cellulose (na-enyere aka ime ka ọrụ hemostatic dịkwuo mma), e jiri Ct mee ihe dị ka ihe ndabere maka NFRCS9,10.A kpụchara Ct n'elu (a hụrụ nhazi ihe nkiri nano dị arọ) ma jikọta ya na ihe mejupụtara hemostatic film-forming (HFFC). HFFC na-arụ ọrụ dị ka matrigel, na-ejide Ct e tinyere n'otu usoro. Nhazi emepụtara na-ebuga nrụgide n'ime usoro gbasaara (eriri na-eme ka eriri sie ike). Ọ na-esiri ike inweta ihe owuwu nanoporous nwere ike mekaniki dị mma site na iji obere mkpokọta polymer. Na mgbakwunye, ọ dịghị mfe ịhazi ebu dị iche iche maka ngwa biomedical dị iche iche.
Foto a na-egosi eserese nke imewe NFRCS dabere na nhazi nku dragonfly (A). Foto a na-egosi ntụnyere nke nhazi nku nke dragonfly (akwara ndị na-agafe na ndị na-agafe ogologo nke nku ahụ jikọtara ọnụ) na foto mikrograf nke Cp NFRCS (B). Nnọchiteanya atụmatụ nke NFRCS.
E ji HFFC mepụta NFRCs dị ka usoro na-aga n'ihu iji dozie oke ndị a dị n'elu. HFFC nwere ọtụtụ polima hemostatic na-akpụ ihe nkiri gụnyere chitosan (dị ka polima hemostatic bụ isi) na methylcellulose (MC), hydroxypropyl methylcellulose (HPMC 50 cp) na polyvinyl alcohol (PVA)) (125 kDa) dị ka polima nkwado nke na-akwalite nhazi thrombus. Mgbakwunye nke polyvinylpyrrolidine K30 (PVP K30) mere ka ikike mmịpụta mmiri nke NFRCS ka mma. E tinyere Polyethylene glycol 400 (PEG 400) iji melite njikọta polima na ngwakọta polima ejikọtara. Ngwakọta hemostatic atọ dị iche iche nke HFFC (Cm HFFC, Ch HFFC na Cp HFFC), ya bụ chitosan na MC (Cm), chitosan na HPMC (Ch), na chitosan na PVA (Cp), ka etinyere na Ct. Nnyocha dị iche iche gbasara njirimara n'ime vitro na n'ime ndụ egosila na NFRCS na-agwọ ọrịa hemostatic na ọnya. Enwere ike iji ihe ndị mejupụtara nke NFRCS na-enye hazie ụdị dị iche iche nke ihe owuwu iji mezuo mkpa ụfọdụ.
Na mgbakwunye, enwere ike ịgbanwe NFRCS dị ka ihe mkpuchi ma ọ bụ mpịakọta iji kpuchie mpaghara mmerụ ahụ niile nke nsọtụ ala na akụkụ ahụ ndị ọzọ. Karịsịa maka mmerụ ahụ n'ụkwụ ọgụ, enwere ike ịgbanwe nhazi NFRCS ka ọ bụrụ ọkara aka ma ọ bụ ụkwụ zuru oke (Foto mgbakwunye S11). Enwere ike ime NFRCS ka ọ bụrụ ihe mkpuchi aka na gluu anụ ahụ, nke enwere ike iji kwụsị ọbara ọgbụgba site na mmerụ ahụ aka dị njọ nke igbu onwe. Ebumnuche anyị bụ isi bụ ịmepụta NFRCS nwere obere polima dịka o kwere mee nke enwere ike ibuga ya na nnukwu ndị mmadụ (n'okpuru oke ịda ogbenye) ma nwee ike itinye ya na ngwa enyemaka mbụ. NFRCS dị mfe, dị irè, na ọnụ ala na imewe, na-abara obodo ndị dị nso uru ma nwee ike inwe mmetụta zuru ụwa ọnụ.
A zụtara Chitosan (n'ibu molekul 80 kDa) na amaranth site na Merck, India. A zụtara Hydroxypropyl methylcellulose 50 Cp, polyethylene glycol 400 na methylcellulose site na Loba Chemie Pvt. LLC, Mumbai. A zụtara Polyvinyl alcohol (n'ibu molekul 125 kDa) (87-90% hydrolyzed) site na National Chemicals, Gujarat. A zụtara Polyvinylpyrrolidine K30 site na Molychem, Mumbai, a zụtara swabs ndị na-adịghị ọcha site na Ramaraju Surgery Cotton Mills Ltd., Tamil Nadu, yana mmiri Milli Q (usoro nhicha mmiri Direct-Q3, Merck, India) dị ka ihe na-ebu ya.
E ji usoro lyophilization11,12 mepụta NFRCS. E ji ihe na-akpali ihe niile dị na HFFC (Tebụl 1). Kwadebe ihe ngwọta 0.5% nke chitosan site na iji 1% acetic acid n'ime mmiri site na ịkpalite ya mgbe niile na 800 rpm na ihe na-akpali ihe. Agbakwunyere oke ibu nke polima ebugoro egosiri na Tebụl 1 na ihe ngwọta chitosan ma kpalie ya ruo mgbe e nwetara ihe ngwọta polima doro anya. Agbakwunyere PVP K30 na PEG 400 na ngwakọta ahụ na ọnụọgụ ego egosiri na Tebụl 1, a nọgidekwara na-akpali ya ruo mgbe e nwetara ihe ngwọta polima viscous doro anya. A na-etinye sonicated bat nke ihe ngwọta polima ahụ ruo nkeji 60 iji wepụ afụfụ ikuku ndị a dọbara na ngwakọta polima ahụ. Dịka egosiri na Foto Mgbakwunye S1(b), e kesara Ct n'otu aka ahụ n'olulu ọ bụla nke efere olulu isii (ebu) nke ejiri 5 ml nke HFFC gbakwunye.
E tinyere sonicated efere nke nwere olulu isii ahụ ruo nkeji iri isii iji mee ka HFFC dị otu ma kesaa ya na netwọk Ct. Mgbe ahụ, kpọnwụọ efere nke nwere olulu isii ahụ na -20°C ruo awa 8-12. E tinyere lyophiliz na efere friza ahụ ruo awa iri anọ na asatọ iji nweta ụdị NFRCS dị iche iche. A na-eji otu usoro ahụ emepụta ụdị na nhazi dị iche iche, dị ka tampons ma ọ bụ tampons cylindrical, ma ọ bụ ụdị ọ bụla ọzọ maka ojiji dị iche iche.
A na-agbaze chitosan a tụrụ nke ọma (80 kDa) (3%) na 1% acetic acid site na iji ihe na-akpali magnet. A na-etinye 1% PEG 400 na ngwọta chitosan wee gwakọta ya ruo nkeji iri atọ. Wụsa ihe ngwọta ahụ n'ime akpa square ma ọ bụ akụkụ anọ wee kpọnwụọ na -80°C ruo awa iri na abụọ. A na-etinye ihe nlele oyi kpọnwụrụ akpọnwụ ruo awa iri anọ na asatọ iji nweta Cs13 nwere oghere.
E jiri Fourier transform infrared spectroscopy (FTIR) (Shimadzu 8400 s FTIR, Tokyo, Japan) mee nnwale NFRCS e mepụtara iji gosi na chitosan na-adakọ na polymer ndị ọzọ14,15. E nwetara FTIR spectra (obosara nke oke spectral site na 400 ruo 4000 cm-1) nke ihe nlele niile a nwalere site na ime nyocha 32.
E jiri usoro Chen et al. 16 kọwara nyochaa ọnụego mmịpụta ọbara (BAR) maka usoro niile, ma nwee obere mgbanwe. E kpọrọ nkụ NFRK ndị mepụtara nke ihe niile dị na oven ikuku na 105°C n'abalị iji wepụ ihe mgbaze fọdụrụ. E tinyere 30 mg NFRCS (ibu ihe nlele mbụ - W0) na 30 mg Ct (njikwa dị mma) n'ime efere dị iche iche nwere ngwakọta sodium citrate 3.8% premix. Na oge edobere, ya bụ, sekọnd 5, 10, 20, 30, 40 na 60, ewepụrụ NFRCS ma hichaa elu ha site na ọbara a na-amịghị amị site na itinye ihe nlele na Ct maka sekọnd 30. A tụlere ibu ikpeazụ nke ọbara nke NFRCS 16 na-amịkọrọ (W1) n'oge ọ bụla. Gbakọọ pasentị BAR site na iji usoro a:
A chọpụtara oge ọbara ji emechi ọbara (BCT) dịka Wang et al. 17 si kwuo. A gbakọrọ oge achọrọ maka ọbara dum (ọbara oke agwakọtara ya na 3.8% sodium citrate) ka ọ kpụọ ọbara n'ihu NFRCS dị ka BCT nke ihe nlele ahụ. E tinyere ihe dị iche iche NFRCS (30 mg) n'ime karama mkpuchi skru 10 ml ma tinye ya na 37°C. A tinyere ọbara (0.5 ml) na karama ahụ ma tinye 0.3 ml nke 0.2 M CaCl2 iji mee ka ọbara ji emechi ọbara. N'ikpeazụ, tụgharịa karama ahụ kwa sekọnd 15 (ruo 180°) ruo mgbe ọ ga-etolite. A na-eme atụmatụ BCT nke ihe nlele ahụ site na ọnụọgụ nke mgbanwe vals17,18. Dabere na BCT, ahọpụtara ihe mejupụtara abụọ kacha mma sitere na NFRCS Cm, Ch na Cp maka ọmụmụ ihe ndị ọzọ gbasara njirimara.
A chọpụtara BCT nke Ch NFRCS na Cp NFRCS site n'itinye usoro Li et al. 19 kọwara. Tinye 15 x 15 mm2 Ch NFRCS, Cp NFRCS, na Cs (njikwa dị mma) n'ime efere Petri dị iche iche (37 °C). E tinyere ọbara nwere 3.8% sodium citrate na 0.2 M CaCl2 n'ime oke olu 10:1 iji malite usoro mkpụkọ ọbara. E tinyere 20 µl nke 0.2 M CaCl2 ngwakọta ọbara oke na elu ihe nlele ahụ ma tinye ya na efere Petri efu. A wụsara ọbara n'ime efere Petri efu na-enweghị Ct. N'oge a kapịrị ọnụ nke nkeji 0, 3, na 5, kwụsị mkpụkọ ọbara site na ịgbakwunye 10 ml nke mmiri deionized (DI) na ihe nlele ahụ nwere efere ahụ na-akpaghasịghị mkpụkọ ọbara ahụ. Erythrocytes na-adịghị agbakọta (erythrocytes) na-enweta hemolysis n'ihu mmiri deionized ma wepụta hemoglobin. A tụrụ Hemoglobin n'oge dị iche iche (HA(t)) na 540 nm (λmax hemoglobin) site na iji UV-Vis spectrophotometer. E weere mmịpụta zuru oke nke hemoglobin (AH(0)) na nkeji 0 nke 20 µl nke ọbara na 10 ml nke mmiri deionized dị ka ọkọlọtọ ntụaka. E jiri otu ọkwa ọbara ahụ gbakọọ nnabata hemoglobin (RHA) nke ọbara kpụkọtara ọnụ site na nha HA(t)/HA(0).
Site na iji ihe nyocha udidi (Texture Pro CT V1.3 Build 15, Brookfield, USA), achọpụtara ihe ndị NFRK nwere nrapado n'ime anụ ahụ mebiri emebi. Pịa efere cylindrical nke nwere ala mepere emepe n'ime akpụkpọ anụ ezi (na-enweghị akwa abụba). E tinyere ihe nlele (Ch NFRCS na Cp NFRCS) site na cannula n'ime ebu cylindrical iji mepụta nrapado na akpụkpọ anụ ezi ahụ. Mgbe a mụsịrị ya nkeji atọ na okpomọkụ ụlọ (RT) (25° C.), e dekọrọ ike nrapado NFRCS na ọnụego mgbe niile nke 0.5 mm/sekọnd.
Isi ihe dị na ihe mkpuchi ịwa ahụ bụ ime ka ọbara kpụọ ma belata mfu ọbara. E jiri usoro e bipụtara na mbụ nyochaa mfu ọbara na NFRCS site na iji obere mgbanwe 19. Mee tube microcentrifuge (2 ml) (dayameta dị n'ime 10 mm) nwere oghere 8 × 5 mm2 n'otu akụkụ nke tube centrifuge (nke na-anọchite anya ọnya mepere emepe). A na-eji NFRCS emechi oghere ahụ ma jiri teepu mechie nsọtụ mpụta. Tinye 20 µl nke 0.2 M CaCl2 na tube microcentrifuge nwere 3.8% sodium citrate premix. Mgbe nkeji iri gachara, ewepụrụ tube microcentrifuge na efere ahụ ma chọpụta mmụba nke oke nke efere ahụ n'ihi ọpụpụ ọbara sitere na NFRK (n = 3). E jiri NFRCS na Cp NFRCS tụnyere Cs.
E kpebiri na NFRCS enweghị mmiri mmiri dabere na usoro Mishra na Chaudhary21 kọwara, mana e nwere obere mgbanwe. Tinye NFRCS n'ime karama Erlenmeyer 100 ml na mmiri 50 ml ma tụgharịa ya ruo sekọnd 60 n'enweghị ihe mkpuchi elu. Nlele anya na ntinye nke ihe nlele maka ịdị ọcha anụ ahụ dabere na nchịkọta.
A mụrụ ike njikọ nke HFFC na Ct site na iji usoro ebipụtara na mbụ yana obere mgbanwe. A tụlere iguzosi ike n'ezi ihe mkpuchi elu site na ikpughe NFRK na ebili mmiri acoustic (mmalite mkpali) n'ihu mmiri milliQ (Ct). E tinyere NFRCS Ch NFRCS na Cp NFRCS ndị emepụtara n'ime beaker jupụtara na mmiri ma gbanye ya maka nkeji 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, na 30, n'otu n'otu. Mgbe akpọchara nkụ, ejiri pasentị dị iche n'etiti ibu mbụ na nke ikpeazụ nke NFRCS gbakọọ pasentị nke mfu nke ihe (HFFC). In vitro BCT kwadokwara ike njikọ ma ọ bụ mfu nke ihe elu. Arụmọrụ nke njikọ HFFC na Ct na-enye coagulation ọbara na mkpuchi na-agbanwe agbanwe n'elu Ct22.
E kpebiri otu ihe NFRCS mepụtara site na BCT nke ihe nlele (30 mg) nke e si n'ebe ndị NFRCS họọrọ n'usoro na-enweghị usoro họrọ. Soro usoro BCT akpọtụrụla iji chọpụta ma NFRCS ọ dabara adaba. Ịdị nso n'etiti ihe nlele ise niile na-eme ka mkpuchi elu dị otu na ntinye HFFC na Ct mesh.
A chọpụtara mpaghara kọntaktị ọbara aha (NBCA) dịka akọwara na mbụ site na mgbanwe ụfọdụ. Mee ka ọbara ahụ jupụta site na ịpịkọta 20 µl nke ọbara n'etiti elu abụọ nke Ct, Ch NFRCS, Cp NFRCS na Cs. Mgbe otu awa gachara, e kewara akụkụ abụọ nke stent ahụ ma jiri aka tụọ mpaghara nke clot ahụ. A na-ewere nkezi uru nke ugboro ugboro atọ dị ka NBCA NFRCS19.
E jiri nyocha Dynamic Vapor Sorption (DVS) nyochaa irè nke NFRCS iji nara mmiri site na gburugburu ebe obibi ma ọ bụ site na ebe mmerụ ahụ nke na-ebute coagulation. DVS na-enyocha ma ọ bụ na-edekọ nnabata uzuoku na mfu na ihe nlele gravimetric site na iji nguzozi dị oke nro na mkpebi oke nke ±0.1 µg. Onye njikwa oke uzuoku eletrọniki na-emepụta nrụgide uzuoku nke akụkụ (iru mmiri dịtụ) gburugburu ihe nlele ahụ site na ịgwakọta gas ndị jupụtara na nke akọrọ. Dịka ntuziaka European Pharmacopeia si dị, dabere na pasentị nke mmiri ndị a na-anabata site na ihe nlele ahụ, e kewara ihe nlele ahụ n'ime ụdị anọ (0–0.012% w/w− nke na-abụghị hygroscopic, 0.2–2% w/w ntakịrị hygroscopic, 2–15% nke hygroscopic nke ọma, na > 15% nke ukwuu hygroscopic)23. Dịka ntuziaka European Pharmacopeia si dị, dabere na pasentị nke mmiri mmiri ndị ihe nlele ahụ na-anabata, e kewara ihe nlele ahụ n'ime ụdị anọ (0–0.012% w/w− nke na-abụghị hygroscopic, 0.2–2% w/w ntakịrị hygroscopic, 2–15% nke hygroscopic nke ọma, na > 15% nke ukwuu hygroscopic)23.Dịka ndụmọdụ nke European Pharmacopoeia si dị, dabere na pasentị nke mmiri mmiri site na ihe nlele ahụ, e kewara ihe nlele ahụ n'ime ụdị anọ (0–0.012% w/w - nke na-abụghị hygroscopic, 0.2–2% w/w ntakịrị hygroscopic, 2–5%).% умеренно гигроскопичен и > 15% очень гигроскопичен)23. % hygroscopic dị ntakịrị na > 15% nke ukwuu hygroscopic)23.根据欧洲药典指南，根据样品吸收水分的百分比，样品分为4 类（0-0.012% w/w- 㸁% w/w 轻微吸湿性,2-15 % 适度吸湿，> 15% 非常吸湿）23.根据 欧洲 药典 指南 ， 根据 吸收 水分 的 百分比 样品 分为 分为 分为 分为 分为 分为 %0-0-0.吸湿 性 、 、 、 0.2-2% W/w 轻微 、 2-15% 适度 吸湿 ，> 15 %非常吸湿）23.Dịka ndụmọdụ nke European Pharmacopoeia si dị, a na-ekewa ihe nlele n'ime klas anọ dabere na pasent nke mmiri mmiri nke ihe nlele ahụ na-amịkọrọ (0-0.012% site na ibu - anaghị adị na hygroscopic, 0.2-2% site na ibu ntakịrị hygroscopic, 2-15% site na ibu).% умеренно гигроскопичен, > 15% очень гигроскопичен) 23. % hygroscopic dị ntakịrị, > 15% nke ukwuu hygroscopic) 23.A chọpụtara arụmọrụ hygroscopic nke NFCS X NFCS na TsN NFCS na DVS TA TGA Q5000 SA na ihe nyocha DVS TA TGA Q5000 SA. N'oge usoro a, enwetara oge ọrụ, iru mmiri dị n'etiti (RH), na oke ihe nlele oge na 25°C24. A na-agbakọ ọdịnaya mmiri site na nyocha oke NFRCS ziri ezi site na iji usoro ndị a:
MC bụ iru mmiri NFRCS. m1 - oke akọrọ nke NSAIDs. m2 bụ oke NFRCS n'oge a na-enye RH.
E jiri nnwale nnabata nitrogen mee atụmatụ oke elu ahụ dum site na iji nitrogen mmiri mmiri mgbe e wepụsịrị ihe nlele ndị ahụ na 25 °C ruo awa 10 (< 7 × 10–3 Torr). E jiri nnwale nnabata nitrogen mee atụmatụ oke elu ahụ dum site na iji nitrogen mmiri mmiri mgbe e wepụsịrị ihe nlele ndị ahụ na 25 °C ruo awa 10 (< 7 × 10–3 Torr). Ndị na-eme ihe nkiri na-eme ka ndị mmadụ na-eme ihe na-eme ka ha na-eme ihe n'ụzọ ziri ezi. образцов при 25 °С в течение 10 ч (< 7 × 10–3 Торр). E jiri nnwale nnabata nitrogen mee atụmatụ oke elu ahụ dum site na iji nitrogen mmiri mmiri mgbe e wụfuru ihe nlele ahụ na 25°C ruo awa 10 (< 7 × 10–3 Torr).在25°C 清空样品10 小时（< 7 × 10-3 Torr）后，使用液氮的氮吸附实验估计总表面积。na okpomọkụ nke 25 ° C Общая площадь poverhnosti опорожнения образцов в течение 10 часов при 25 ° C (<7 × 10-3 торр). E jiri nnwale nnabata nitrogen na nitrogen mmiri mee atụmatụ oke elu ahụ dum mgbe e wụfuru ihe nlele ahụ ruo awa iri na 25°C (< 7 x 10-3 torr).E jiri ngwanrọ RS 232 chọpụta oke elu, olu oghere na nha oghere NFRCS.
Kwadebe 5% RBCs (nri dị ka ihe na-agbaze) site na ọbara dum. Wee bufee otu HFFC (0.25 ml) na efere olulu 96 na oke RBC 5% (0.1 ml). Kpoo ngwakọta ahụ na 37°C maka nkeji iri anọ. A na-ewere ngwakọta nke mkpụrụ ndụ ọbara uhie na serum dị ka njikwa dị mma, yana ngwakọta nke saline na mkpụrụ ndụ ọbara uhie dị ka njikwa na-adịghị mma. A chọpụtara hemagglutination dịka nha Stajitzky si dị. Ihe atụ ndị a tụrụ aro bụ ndị a: + + + + + mkpokọta granular dị oke; + + + pads ala dị nro nwere nsọtụ gbagọrọ agbagọ; + + pads ala dị nro nwere nsọtụ dọwara adọwa; + mgbaaka uhie dị warara gburugburu nsọtụ pads dị nro; – (na-adịghị mma) bọtịnụ uhie dị iche iche 12 n'etiti olulu mmiri dị ala.
A mụrụ usoro nke International Organization for Standardization (ISO) (ISO10993-4, 1999)26,27. Usoro gravimetric nke Singh na ndị otu ya kọwara mere obere mgbanwe iji chọpụta nhazi thrombus n'ihu ma ọ bụ n'elu NFRCS. E tinyere 500 mg nke Cs, Ch NFRCS na Cp NFRCS na phosphate buffered saline (PBS) ruo awa 24 na 37°C. Mgbe awa 24 gachara, ewepụrụ PBS ma jiri 2 ml nke ọbara nwere 3.8% sodium citrate gwọọ NFRCS. N'elu NFRCS, tinye 0.04 ml nke 0.1 M CaCl2 na ihe nlele ndị a kpọchiri akpọchi. Mgbe nkeji 45 gachara, etinyere 5 ml nke mmiri a gwakọtara agwakọta iji kwụsị coagulation. E jiri 36-38% formaldehyde ngwọta gwọọ globules ndị ahụ e tinyere formaldehyde. E jiri formaldehyde kpụọ ha ma tụọ ha. A na-eme atụmatụ pasentị nke thrombosis site na ịgbakọ ibu nke iko ahụ na-enweghị ọbara na ihe nlele (njikwa na-adịghị mma) na iko ahụ na ọbara (njikwa dị mma).
Dịka nkwenye mbụ, e jiri igwe onyonyo anya hụ ihe nlele ndị ahụ n'okpuru igwe onyonyo anya iji ghọta ikike mkpuchi elu HFFC, njikọ Ct na netwọk Ct nwere ike ime oghere. E jiri mma agụba scalpel kpụchaa akụkụ dị gịrịgịrị nke Ch na Cp sitere na NFRCS. E tinyere akụkụ ahụ si na ya pụta na slaịd iko, kpuchie ya na mkpuchi, ma jiri gluu dozie nsọtụ ya. E lere slaịd ndị ahụ e kwadebere n'okpuru igwe onyonyo anya ma see foto na nnukwu ihe dị iche iche.
E jiri igwe onyonyo fluorescence mee ihe onyonyo nke ihe ndị e ji echekwa polima na netwọk Ct dabere na usoro Rice et al.29 kọwara. E jiri agba fluorescent (amaranth) gwakọta ihe mejupụtara HFFC e ji mee ihe maka ọgwụ ahụ, e wee kwadebe NFRCS (Ch & Cp) dịka usoro e kwuru na mbụ si dị. E jiri agba fluorescent (amaranth) gwakọta ihe mejupụtara HFFC e ji mee ihe maka ọgwụ ahụ, e wee kwadebe NFRCS (Ch & Cp) dịka usoro e kwuru na mbụ si dị.E jiri agba fluorescent (amaranth) gwakọta ihe mejupụtara HFFC e ji mee ihe maka nhazi ahụ, e wee nweta NFRCS (Ch na Cp) dịka usoro e kwuru na mbụ si dị.将用于配方的HFFC 组合物与荧光染料（苋菜）混合，并照前面提到的方法NFRCS）将用于配方的HFFC 组合物与荧光染料（苋菜）混合，并照前面提到的方法NFRCS）A gwakọtara ihe mejupụtara HFFC ejiri mee ihe n'ime usoro ahụ na agba fluorescent (Amaranth) ma nweta NFRCS (Ch na Cp), dịka ekwuru na mbụ.E bepụrụ akụkụ dị gịrịgịrị nke NFRK site na ihe nlele ndị e nwetara, tinye ha na slaịd iko, ma kpuchie ha na mpempe mkpuchi. Lelee slaịd ndị a kwadebere n'okpuru microscope fluorescent site na iji nzacha akwụkwọ ndụ akwụkwọ ndụ (310-380 nm). E sere foto na mmụba ugboro anọ iji ghọta mmekọrịta Ct na oke nchekwa polymer na netwọk Ct.
E jiri igwe microscope ike atọm (AFM) chọpụta elu ala nke NFRCS Ch na Cp yana cantilever TESP dị nkọ nke ukwuu n'ụdị ịpị aka: 42 N/m, 320 kHz, ROC 2-5 nm, Bruker, Taiwan. Ejiri ngwanrọ (Scanning Probe Image Processor) chọpụta oke elu site na iji mgbọrọgwụ nkezi square (RMS). E gosipụtara ebe dị iche iche NFRCS na onyonyo 3D iji lelee nha anya elu. A na-akọwa ọdịiche ọkọlọtọ nke akara maka mpaghara enyere dị ka oke elu. E jiri nha RMS tụọ oke elu nke NFRCS31.
E jiri FESEM, SU8000, HI-0876-0003, Hitachi, Tokyo mee nnyocha ndị dabere na FESEM iji ghọta ọdịdị elu nke Ch NFRCS na Cp NFRCS, nke gosiri BCT ka mma karịa Cm NFRCS. E mere nnyocha FESEM dịka usoro Zhao et al. 32 kọwara si dị, e mekwara obere mgbanwe. E jiri 20 µl nke 3.8% sodium citrate gwakọta NFRCS na Cp NFRCS gwakọta NFRCS 20 ruo 30 mg nke sodium citrate e tinyere na ọbara oke. E tinyere 20 μl nke 0.2 M CaCl2 na ihe nlele ndị a gwọrọ ọbara iji malite coagulation ma tinye ihe nlele ndị ahụ n'ime okpomọkụ ụlọ maka nkeji iri. Na mgbakwunye, e wepụrụ erythrocytes gabigara ókè n'elu NFRCS site na ịsacha na saline.
E jiri glutaraldehyde 0.1% gwọọ ihe nlele ndị sochirinụ wee kpọọ nkụ n'ime oven ikuku ọkụ na 37°C iji wepụ mmiri mmiri. E kpuchiri ihe nlele ndị kpọrọ nkụ ma nyochaa 32. Foto ndị ọzọ e nwetara n'oge nyocha ahụ bụ nhazi klọm n'elu eriri owu nke ọ bụla, ntinye polymer n'etiti Ct, ọdịdị erythrocyte (ọdịdị), iguzosi ike n'ezi ihe nke klọm, na ọdịdị erythrocyte n'ihu NFRCS. E nyochachara mpaghara NFRCS na-enweghị ọgwụgwọ na mpaghara NFRCS e tinyere ọbara na Ch na Cp maka ion elemental (sodium, potassium, nitrogen, calcium, magnesium, zinc, copper na selenium)33. Tụlee pasentị ion elemental n'etiti ihe nlele e nyere ọgwụgwọ na nke a na-enyeghị ọgwụgwọ iji ghọta mkpokọta ion elemental n'oge mmepụta klọm na homogeneity nke klọm.
E ji FESEM chọpụta ọkpụrụkpụ nke mkpuchi elu Cp HFFC n'elu Ct. E si na usoro ahụ bepụ akụkụ obe nke Cp NFRCS ma kpuchie sputter. FESEM hụrụ ihe nlele mkpuchi sputter sitere na ya, a tụkwara ọkpụrụkpụ nke mkpuchi elu ahụ 34, 35, 36.
X-ray micro-CT na-enye onyonyo 3D dị elu nke na-anaghị emebi emebi ma na-enye gị ohere ịmụ banyere nhazi nhazi nke NFRK. Micro-CT na-eji ụzarị X-ray na-agafe na sample ahụ iji dekọọ ọnụọgụgụ mbelata ahịrị mpaghara nke radieshọn X na sample ahụ, nke na-enyere aka inweta ozi morphological. E lere ọnọdụ ime nke Ct na Cp NFRCS na Cp NFRCS nke a gwọrọ n'ọbara site na micro-CT iji ghọta arụmọrụ nnabata na mkpụkọ ọbara n'ihu NFRCS37,38,39. E jiri micro-CT (V|tome|x S240, Phoenix, Germany) wughachi usoro 3D nke samples Cp NFRCS nke a gwọrọ n'ọbara na nke a gwọrọ n'enweghị ọgwụgwọ. Site na iji micro-CT (V|tome|x S240, Phoenix, Germany) wughachi usoro 3D nke samples Cp NFRCS nke a gwọrọ n'ọbara na nke a gwọrọ n'enweghị ọgwụgwọ). Site na iji ngwanrọ VG STUDIO-MAX nke 2.2, e si n'akụkụ dị iche iche were ọtụtụ onyonyo X-ray (nke kachasị mma mkpuchi 360°) mepụta onyonyo 3D maka NFRCS. E wughachiri data projection anakọtara ka ọ bụrụ onyonyo volumetric 3D site na iji ngwanrọ 3D ScanIP Academic dị mfe kwekọrọ.
Tinyere nke a, iji ghọta nkesa nke mkpụkọ ọbara ahụ, etinyere 20 µl nke ọbara citrate agwakọtara na 20 µl nke 0.2 M CaCl2 na NFRCS iji mee ka ọbara kpụọ. A na-ahapụ ihe nlele ndị a kwadebere ka ha sie ike. E jiri 0.5% glutaraldehyde gwọọ elu NFRK ma kpọọ nkụ n'ime oven ikuku ọkụ na 30–40°C maka nkeji iri atọ. E nyochachara mkpụkọ ọbara ahụ e kpụrụ na NFRCS, wughachi ya ọzọ, ma hụ foto 3D nke mkpụkọ ọbara ahụ.
E mere nnwale nje bacteria na Cp NFRCS (nke kacha mma ma e jiri ya tụnyere Ch NFRCS) site na iji usoro akọwara na mbụ na obere mgbanwe. E jiri obere nje bacteria atọ dị iche iche [S.aureus (bacteria gram-positive), E.coli (bacteria gram-negative) na Candida ọcha (C.albicans)] chọpụta ọrụ nje bacteria nke Cp NFRCS na Cp HFFC na-eto na agar na efere Petri na incubator. Tinye 50 ml nke ihe mgbochi nje bacteria a gwakọtara agwakọta na mkpokọta nke 105-106 CFU ml-1 na ihe mgbochi agar. Wụsa ihe mgbochi ahụ n'ime efere Petri ma hapụ ya ka ọ sie ike. E mere olulu mmiri n'elu efere agar iji jupụta na HFFC (olulu mmiri atọ maka HFFC na 1 maka njikwa adịghị mma). Tinye 200 µl HFFC na olulu mmiri atọ na 200 µl pH 7.4 PBS na olulu mmiri nke anọ. N'akụkụ nke ọzọ nke efere petri, tinye diski NFRCS Cp nke 12 mm n'elu agar siri ike ma tinye mmiri mmiri na PBS (pH 7.4). A na-ewere mbadamba Ciprofloxacin, ampicillin na fluconazole dị ka ụkpụrụ ntụaka maka Staphylococcus aureus, Escherichia coli na Candida albicans. Tụọ mpaghara mgbochi ahụ n'aka ma were onyonyo dijitalụ nke mpaghara mgbochi ahụ.
Mgbe e mechara nkwenye omume ụlọ ọrụ, e mere nnyocha ahụ na Kasturba Medical College of Education and Research na Manipal, Karnataka, na ndịda India. Kọmitii Ụkpụrụ Omume nke Kasturba Medical College, Manipal, Karnataka enyochaala ma kwado usoro nnwale TEG in vitro (IEC: 674/2020). E si n'aka ndị na-enye ọbara afọ ofufo (ndị dị afọ 18 ruo 55) kpọbata ndị mmadụ n'ụlọ akụ ọbara ụlọ ọgwụ. Na mgbakwunye, e nwetara akwụkwọ nkwenye amamihe sitere n'aka ndị ọrụ afọ ofufo maka nchịkọta nke ihe nlele ọbara. E jiri TEG (N-TEG) ​​mụọ mmetụta nke usoro Cp HFFC na ọbara dum agwakọtara na sodium citrate. A maara N-TEG nke ọma maka ọrụ ya na mweghachi nlekọta, nke na-akpata nsogbu maka ndị dọkịta n'ihi ike nke igbu oge dị ukwuu na nsonaazụ (ule coagulation na-aga n'ihu). E jiri ọbara dum mee nyocha N-TEG. E nwetara nkwenye amamihe na akụkọ ahụike zuru ezu site n'aka ndị niile sonyere. Ọmụmụ ihe ahụ agụnyeghị ndị sonyere nwere nsogbu hemostatic ma ọ bụ thrombotic dị ka afọ ime/mgbe ịmụ nwa gasịrị ma ọ bụ ọrịa imeju. E wepụkwara ndị na-aṅụ ọgwụ ndị na-emetụta coagulation cascade na ọmụmụ ihe ahụ. E mere nnwale ụlọ nyocha bụ isi (hemoglobin, oge prothrombin, thromboplastin activated na ọnụọgụ platelet) na ndị niile sonyere dịka usoro ọkọlọtọ si dị. N-TEG na-achọpụta viscoelasticity nke clot clot, nhazi clot mbụ, mmekọrịta nke ihe ndị dị n'ime ya, ike clot, na lysis clot. Nyocha N-TEG na-enye data eserese na ọnụọgụ na mmetụta mkpokọta nke ọtụtụ ihe sel na plasma. E mere nyocha N-TEG na mpịakọta abụọ dị iche iche nke Cp HFFC (10 µl na 50 µl). N'ihi ya, etinyere 1 ml nke ọbara zuru oke yana citric acid na 10 μl nke Cp HFFC. Tinye 1 ml (Cp HFFC + ọbara citrate), 340 µl ọbara agwakọtara na 20 µl 0.2 M CaCl2 nwere efere TEG. Mgbe nke ahụ gasịrị, e tinyere efere TEG n'ime TEG® 5000, US iji tụọ R, K, angle alpha, MA, G, CI, TPI, EPL, LY 30% nke ihe nlele ọbara n'ihu Cp HFFC41.
Kọmitii Ethics of Animal Ethics nke Institutional (IAEC), Kasturba School of Medicine, Manipal Institute of Higher Education, Manipal (IAEC/KMC/69/2020) nyochachara ma kwado usoro ọmụmụ in vivo. Emere nnwale anụmanụ niile dịka ndụmọdụ nke Kọmitii maka Njikwa na Nlekọta nke Nnwale Anụmanụ (CPCSEA) si dị. Emere ọmụmụ NFRCS niile (2 × 2 cm2) na oke Wistar nwanyị (nke dị 200 ruo 250 g). E mere ka anụmanụ niile nabata na okpomọkụ nke 24-26°C, anụmanụ niile nwere ohere ịnweta nri na mmiri nkịtị na libitum. E kewara anụmanụ niile n'ime otu dị iche iche n'enweghị usoro, otu ọ bụla nwere anụmanụ atọ. Emere ọmụmụ ihe niile dịka Ọmụmụ Anụmanụ si dị: Akụkọ nke Nnwale In Vivo 43. Tupu ọmụmụ ihe a, a na-enye anụmanụ ndị ahụ ọgwụ mgbu site na iji ngwakọta nke 20-50 mg nke ketamine (kwa kilogram 1 nke ibu ahụ) na 2-10 mg nke xylazine (kwa kilogram 1 nke ibu ahụ). Mgbe ọmụmụ ihe ahụ gasịrị, a gbakọrọ olu ọbara site n'inyocha ọdịiche dị n'etiti ibu mbụ na nke ikpeazụ nke ihe nlele ahụ, a na-ewere nkezi uru e nwetara site na ule atọ ahụ dị ka olu ọbara nke ihe nlele ahụ.
E tinyere ihe nlereanya ịkpụpụ ọdụ oke iji ghọta ikike NFRCS nwere iji gbanwee ọbara ọgbụgba n'oge mmerụ ahụ, ọgụ, ma ọ bụ ihe mberede okporo ụzọ (ụdị mmerụ ahụ). Bee 50% nke ọdụ ahụ na mma scalpel wee tinye ya n'ikuku ruo sekọnd 15 iji hụ na ọbara ọgbụgba nkịtị. Na mgbakwunye, e tinyere ihe nlele ule n'ọdụ oke site na itinye nrụgide (Ct, Cs, Ch NFRCS na Cp NFRCS). A kọrọ ọbara ọgbụgba na PCT maka ihe nlele ule (n = 3)17,45.
A nyochachara irè nke njikwa nrụgide NFRCS n'ọgụ na ihe nlereanya nke akwara femoral dị elu. A na-ekpughe akwara femoral ahụ, a na-akụ ya na trocar 24G, ma na-agba ya ọbara n'ime sekọnd 15. Mgbe a hụrụ ọbara ọgbụgba a na-achịkwaghị achịkwa, a na-etinye ihe nlele ule ahụ n'ebe a na-akụ ya ma tinye nrụgide. Ozugbo e tinyere ihe nlele ule ahụ, a dekọrọ oge mkpọchi ọbara ma hụ arụmọrụ hemostatic maka nkeji 5 sochirinụ. E mekwara otu usoro ahụ na Cs na Ct46.
Dowling na ndị otu ya 47 tụrụ aro ụdị mmerụ ahụ imeju iji chọpụta ike hemostatic nke ihe hemostatic n'ihe gbasara ọbara ọgbụgba n'ime ịwa ahụ. E dekọrọ BCT maka ihe nlele Ct (njikwa na-adịghị mma), usoro Cs (njikwa dị mma), ihe nlele Ch NFRCS, na ihe nlele Cp NFRCS. E gosipụtara suprahepatic vena cava nke oke ahụ site na ime laparotomy etiti. Mgbe nke ahụ gasịrị, e jiri mkpa bepụ akụkụ dị anya nke lobe aka ekpe. Mee mbepụ n'ime imeju na mma scalpel ma hapụ ya ka ọ gbaa ọbara ruo sekọnd ole na ole. E tinyere ihe nlele Ch NFRCS na Cp NFRCS nke a tụrụ nke ọma n'elu ebe mebiri emebi na-enweghị nrụgide dị mma ma dekọọ BCT. Otu njikwa (Ct) wee tinye nrụgide wee soro Cs 30 s47 na-agbajighị mmerụ ahụ.
E jiri ụdị ọnya ịwa ahụ mee nnwale ọgwụgwọ ọnya n'ime ahụ iji nyochaa njirimara ọgwụgwọ ọnya nke NFRCSs e mepụtara site na polymer. A họọrọ ma mee ụdị ọnya ịwa ahụ dịka usoro ebipụtara na mbụ yana obere mgbanwe19,32,48. A na-eme ka anụmanụ niile nwee ahụ mgbu dịka akọwara na mbụ. Jiri mkpịsị biopsy (12 mm) mee mbepụ miri emi n'akpụkpọ azụ. E yikwasị ebe ọnya ndị a kwadebere na Cs (njikwa dị mma), Ct (na-aghọta na ákwà owu na-egbochi ọgwụgwọ), Ch NFRCS na Cp NFRCS (otu nnwale) na njikwa na-adịghị mma na-enweghị ọgwụgwọ ọ bụla. N'ụbọchị ọ bụla nke ọmụmụ ihe ahụ, a tụrụ mpaghara ọnya ahụ na oke niile. Jiri igwefoto dijitalụ see foto nke ebe ọnya ahụ wee tinye ihe mkpuchi ọhụrụ. A tụrụ pasentị nke mmechi ọnya site na usoro a:
Dabere na pasentị nke mmechi ọnya n'ụbọchị nke iri na abụọ nke ọmụmụ ihe ahụ, a kpụpụrụ akpụkpọ anụ oke nke otu kacha mma ((Cp NFRCS) na otu njikwa) ma mụọ ya site na H&E staining na Masson's trichrome staining. Dabere na pasentị nke mmechi ọnya n'ụbọchị nke iri na abụọ nke ọmụmụ ihe ahụ, a kpụpụrụ akpụkpọ anụ oke nke otu kacha mma ((Cp NFRCS) na otu njikwa) ma mụọ ya site na H&E staining na Masson's trichrome staining.Dabere na pasentị nke mmechi ọnya n'ụbọchị nke iri na abụọ nke ọmụmụ ihe ahụ, a kpụpụrụ akpụkpọ ahụ nke òké nke otu kacha mma ((Cp NFRCS) na otu njikwa) ma lelee ya site na iji hematoxylin-eosin na trichrome nke Masson.根据研究第12天的伤口闭合百分比，切除最佳组((Cp) NFRCS)和对照组)的大鼠皮肤，进行H&E染色和Masson三色染色研究。根据研究第12天的伤口闭合百分比，切除最佳组((Cp) NFRCS)和对照组)的大鼠皮肤，进行H&E染色和眳组）A chụpụrụ oke ndị nọ n'òtù kacha mma ((Cp NFRCS) na ndị otu njikwa) maka ntụpọ hematoxylin-eosin na ntụpọ trichrome nke Masson dabere na pasentị mmechi ọnya na ụbọchị nke 12 nke ọmụmụ ihe ahụ.E mere usoro agba agba emere dịka usoro ndị e kwuru na mbụ49,50 si dị. N'oge na-adịghị anya, mgbe etinyere ya na 10% formalin, e jiri usoro mmanya ndị a kpụchara akpụcha mee ka mmiri ghara ịdị n'ime ihe nlele ahụ. Jiri microtome nweta akụkụ dị gịrịgịrị (5 µm ọkpụrụkpụ) nke anụ ahụ a kpụchara akpụcha. E jiri hematoxylin na eosin gwọọ akụkụ dị gịrịgịrị nke njikwa na Cp NFRCS iji mụọ mgbanwe histopathological. E jiri ntụpọ trichrome nke Masson chọpụta nhazi nke eriri collagen. Ndị ọkachamara n'ọrịa nyochachara nsonaazụ ndị e nwetara n'enweghị nghọta.
A mụrụ nkwụsi ike nke ihe nlele Cp NFRCS n'okpomọkụ ụlọ (25°C ± 2°C/60% RH ± 5%) ruo ọnwa 1251. A lere anya ma nwalee Cp NFRCS (mgbanwe agba elu na uto nje bacteria) maka iguzogide iyi mpịachi na BCT dịka usoro ndị dị n'elu si dị na ngalaba ihe na usoro.
E lere anya n'otú Cp NFRCS si agbanwe agbanwe na otú o si agbanwe agbanwe site n'ịkwadebe Cp NFRCS nke nha ya bụ 15×15 cm2. Na mgbakwunye, e wepụrụ ihe nlele 30 mg (n = 5) site na akụkụ dị iche iche nke Cp NFRCS ma nyochaa BCT nke ihe nlele ndị a mụrụ dịka akọwara na mbụ na ngalaba Usoro.
Anyị agbalịala ịmepụta ụdị na nhazi dị iche iche site na iji ihe mejupụtara Cp NFRCS maka ọtụtụ ojiji ahụike. Ụdị ma ọ bụ nhazi ndị dị otú ahụ gụnyere swab conical maka ọbara ọgbụgba imi, usoro ezé, na swab cylindrical maka ọbara ọgbụgba ikpu.
A na-egosipụta nhazi data niile dị ka nkezi ± ọkọlọtọ divideration ma ANOVA jiri Prism 5.03 (GraphPad, San Diego, CA, USA) nyochaa ha, wee soro ule ntụnyere Bonferroni (*p<0.05).
Usoro niile e mere n'ọmụmụ ihe mmadụ kwekọrọ n'ụkpụrụ nke Institute na National Research Council, yana Nkwupụta nke Helsinki 1964 na mgbanwe ndị sochirinụ, ma ọ bụ ụkpụrụ omume yiri ya. A gwara ndị niile sonyere gbasara atụmatụ nke ọmụmụ ihe ahụ na ọdịdị afọ ofufo ya. Data ndị sonyere na-anọgide na nzuzo ozugbo anakọtara ya. Kọmitii Omume nke Kasturba Medical College, Manipal, Karnataka enyochaala ma kwado usoro nnwale TEG na vitro (IEC: 674/2020). Ndị ọrụ afọ ofufo bịanyere aka na nkwenye ama ama iji nakọta ihe nlele ọbara.
E mere usoro niile e mere n'ọmụmụ anụmanụ dịka Kastuba Faculty of Medicine, Manipal Institute of Higher Education, Manipal (IAEC/KMC/69/2020) si kwuo. E mere nnwale anụmanụ niile e mere dịka ntuziaka nke Kọmitii maka Njikwa na Nlekọta nke Nnwale Anụmanụ (CPCSEA) si dị. Ndị edemede niile na-agbaso ntuziaka ARRIVE.
E nyochachara ma tụnyere spectra FTIR nke NFRCS niile na spectra chitosan egosiri na Foto 2A. Ọnụ ọgụgụ spectra nke chitosan (edere) na 3437 cm-1 (ntinye OH na NH, overlapse), 2945 na 2897 cm-1 (ntinye CH), 1660 cm-1 (nrịba NH2), 1589 cm-1 (ngbatị N-H), 1157 cm-1 (ngbatị bridge O-), 1067 cm-1 (ngbatị C-O, hydroxyl nke abụọ), 993 cm-1 (ngbatị CO, Bo-OH) 52.53.54. Tebụl Mgbakwunye S1 na-egosi ụkpụrụ FTIR NFRCS absorption spectrum ​​​​maka chitosan (onye na-akọ akụkọ), chitosan dị ọcha, Cm, Ch, na Cp. Nleba anya FTIR nke NFRCS niile (Cm, Ch na Cp) gosiri otu ihe eji amata ihe dị ka chitosan dị ọcha na-enweghị mgbanwe dị mkpa (Fig. 2A). Nsonaazụ FTIR kwadoro enweghị mmekọrịta kemịkalụ ma ọ bụ anụ ahụ n'etiti polymers ejiri mepụta NFRCS, na-egosi na polymers ejiri mee ihe adịghị arụ ọrụ.
N'ime vitro, njirimara nke Cm NFRCS, Ch NFRCS, Cp NFRCS na Cs. (A) na-anọchite anya FTIR spectra nke ihe mejupụtara chitosan na Cm NFRCS, Ch NFRCS na Cp NFRCS n'okpuru nrụgide. (B) a) Ọnụego nnabata ọbara zuru oke nke NFRCS Cm, Ch, Cp, na Cg (n = 3); Ihe nlele Ct gosiri BAR dị elu n'ihi na swab owu nwere arụmọrụ nnabata dị elu; b) Ọbara mgbe ịmịnye ọbara gasịrị Ihe osise nke ihe nlele etinyere. Ngosipụta eserese nke BCT nke ihe nlele ule C (Cp NFRCS nwere BCT kacha mma (15 s, n = 3)). E gosiri data dị na C, D, E, na G dị ka nkezi ± SD, ebe ogwe njehie na-anọchite anya SD, ***p < 0.0001. E gosiri data dị na C, D, E, na G dị ka nkezi ± SD, ebe ogwe njehie na-anọchite anya SD, ***p < 0.0001. Данные в C, D, E и G представлены как среднее ± стандартное отклонение, *** p <0,0001. A na-egosi data dị na C, D, E, na G dị ka nkezi ± ọkọlọtọ mgbanwe, ebe ogwe njehie na-anọchite anya ọkọlọtọ mgbanwe, ***p<0.0001. C、D、E 和G 中的数据显示为平均值± SD，误差线代表SD，***p <0.0001. C、D、E 和G 中的数据显示为平均值± SD，误差线代表SD，***p <0.0001. Данные в C, D, E и G показаны как среднее значение ± стандартное отклонение, *** p <0,0001. A na-egosi data dị na C, D, E, na G dị ka nkezi ± nkezi nkebi, ogwe njehie na-anọchite anya nkezi nkebi, ***p<0.0001.