Chosakaniza chatsopano cha inline static static chapangidwa kuti chikwaniritse zofunikira za high performance liquid chromatography (HPLC) ndi ultra high performance liquid chromatography (HPLC ndi UHPLC).Kusakanikirana koyipa kwa magawo awiri kapena kupitilira apo kungapangitse kuti pakhale chiwopsezo chambiri, chomwe chimachepetsa chidwi.Kusakanikirana kofanana kwamadzimadzi awiri kapena kuposerapo okhala ndi voliyumu yocheperako yamkati ndi miyeso yakuthupi ya chosakanizira chosasunthika kumayimira muyezo wapamwamba kwambiri wa chosakanizira chokhazikika.Chosakaniza chatsopano cha static chimakwaniritsa izi pogwiritsa ntchito ukadaulo watsopano wosindikiza wa 3D kuti apange mawonekedwe apadera a 3D omwe amapereka kusanganikirana kwabwino kwa hydrodynamic static ndikuchepetsa kwambiri kuchuluka kwa base sine wave pagawo lamkati la osakaniza.Kugwiritsa ntchito 1/3 ya voliyumu yamkati ya chosakaniza wamba kumachepetsa mafunde a sine ndi 98%.Chosakanizacho chimakhala ndi njira zolumikizirana za 3D zokhala ndi madera osiyanasiyana am'mbali komanso utali wanjira pomwe madziwo amadutsa ma geometri ovuta a 3D.Kuphatikizana ndi njira zingapo zowawa, kuphatikiza chipwirikiti chakomweko ndi ma eddies, kumabweretsa kusakanikirana kwa sikelo ya micro, meso ndi macro.Chosakaniza chapaderachi chidapangidwa pogwiritsa ntchito ma computational fluid dynamics (CFD).Deta yoyezetsa yomwe yaperekedwa ikuwonetsa kuti kusakanikirana kwabwino kumatheka ndi voliyumu yaying'ono yamkati.
Kwa zaka zoposa 30, chromatography yamadzimadzi yakhala ikugwiritsidwa ntchito m'mafakitale ambiri, kuphatikizapo mankhwala, mankhwala ophera tizilombo, kuteteza chilengedwe, forensics, ndi kusanthula mankhwala.Kutha kuyeza magawo miliyoni kapena kuchepera ndikofunikira pakukula kwaukadaulo mumakampani aliwonse.Kusakanizikana kosakwanira bwino kumabweretsa kuchepa kwa ma sign-to-phokoso, zomwe zimakwiyitsa gulu la chromatography malinga ndi malire ozindikira komanso kumva.Posakaniza zosungunulira ziwiri za HPLC, nthawi zina zimakhala zofunikira kukakamiza kusakaniza ndi njira zakunja kuti zikhale ndi homogenize zosungunulira ziwirizo chifukwa zosungunulira zina sizikusakanikirana bwino.Ngati zosungunulira sizisakanizidwa bwino, kuwonongeka kwa chromatogram ya HPLC kumatha kuchitika, kuwonekera ngati phokoso lochulukirapo komanso / kapena mawonekedwe osawoneka bwino.Ndi kusakanikirana kosakwanira, phokoso loyambira lidzawoneka ngati mafunde a sine (kukwera ndi kugwa) kwa chizindikiro cha detector pakapita nthawi.Nthawi yomweyo, kusakanikirana koyipa kungayambitse kukulitsa ndi nsonga za asymmetric, kuchepetsa magwiridwe antchito, mawonekedwe apamwamba, komanso kusanja kwapamwamba.Makampaniwa azindikira kuti makina osakanikirana ndi ma tee static ndi njira zowonjezera malirewa ndikulola ogwiritsa ntchito kukwaniritsa malire ozindikira (zomverera).Chosakaniza chabwino cha static chimaphatikiza phindu la kusakaniza kwakukulu, kutsika kwakufa kwakufa ndi kutsika kwapang'onopang'ono ndi kutsika kochepa komanso kutulutsa kwakukulu kwa dongosolo.Kuonjezera apo, pamene kusanthula kumakhala kovuta kwambiri, akatswiri ayenera kugwiritsa ntchito nthawi zonse zosungunulira za polar komanso zovuta kusakaniza.Izi zikutanthauza kuti kusakaniza kwabwinoko ndikofunikira pakuyezetsa mtsogolo, kukulitsa kufunikira kwa mapangidwe apamwamba osakaniza ndi magwiridwe antchito.
Mott posachedwapa wapanga mitundu yatsopano ya PerfectPeak TM inline static mixers yokhala ndi mavoliyumu atatu amkati: 30 µl, 60 µl ndi 90 µl.Makulidwe awa amaphimba kuchuluka kwa ma voliyumu ndi kusakanikirana komwe kumafunikira pamayeso ambiri a HPLC komwe kusakanikirana bwino komanso kubalalitsidwa kochepa kumafunikira.Mitundu itatu yonseyi ndi 0.5 ″ m'mimba mwake ndipo imapereka magwiridwe antchito apamwamba pamapangidwe apakatikati.Amapangidwa ndi chitsulo chosapanga dzimbiri cha 316L, chodutsa chifukwa cha inertness, koma titaniyamu ndi zina zosagwirizana ndi dzimbiri komanso ma aloyi achitsulo achitsulo amapezekanso.Zosakaniza izi zimakhala ndi mphamvu yogwira ntchito kwambiri mpaka 20,000 psi.Pa mkuyu.1a ndi chithunzi cha chosakanizira cha 60 µl Mott static chopangidwa kuti chizitha kusakaniza bwino kwinaku mukugwiritsa ntchito voliyumu yaying'ono yamkati kuposa zosakaniza wamba zamtunduwu.Kapangidwe katsopano ka static kosakaniza kameneka kamagwiritsa ntchito ukadaulo watsopano wopangira zowonjezera kuti apange mawonekedwe apadera a 3D omwe amagwiritsa ntchito kuyenda pang'ono mkati kuposa chosakanizira chilichonse chomwe chimagwiritsidwa ntchito pamakampani opanga ma chromatography kuti akwaniritse kusakanikirana kosasunthika.Amenewa mixers zigwirizana interconnected atatu azithunzithunzi otaya ngalande ndi osiyana mtanda-gawo madera osiyana njira utali ngati madzi mitanda zovuta zojambula zotchinga mkati.Pa mkuyu.Chithunzi 1b chikuwonetsa chojambula cha chosakanizira chatsopanocho, chomwe chimagwiritsa ntchito makina ophatikizira a HPLC amtundu wa 10-32 wolowera ndi kutulutsa, ndipo ali ndi malire abuluu a doko losakanizira lamkati lovomerezeka.Madera osiyanasiyana odutsa njira zamkati ndikusintha kwamayendedwe oyenda mkati mwa voliyumu yotuluka mkati mwake amapanga zigawo za chipwirikiti ndi kutuluka kwa laminar, zomwe zimapangitsa kusakanikirana kwa milingo yaying'ono, maso ndi macro.Mapangidwe a chosakanizira chapadera ichi adagwiritsa ntchito ma computational fluid dynamics (CFD) kusanthula machitidwe oyenda ndikuwongolera makonzedwe asanayambe kuyesedwa koyesa m'nyumba ndikuwunika kwamakasitomala.Kupanga zowonjezera ndi njira yosindikizira zigawo za 3D za geometric mwachindunji kuchokera ku zojambula za CAD popanda kufunikira kwa makina achikhalidwe (makina ophera, lathes, ndi zina zotero).Zosakaniza zatsopanozi zimapangidwira kuti zipangidwe pogwiritsa ntchito njirayi, pomwe thupi losakaniza limapangidwa kuchokera ku zojambula za CAD ndipo zigawo zake zimapangidwira (zosindikizidwa) zosanjikiza ndi zosanjikiza pogwiritsa ntchito zowonjezera zowonjezera.Apa, chitsulo chosanjikiza cha zitsulo pafupifupi ma microns 20 chimayikidwa, ndipo laser yoyendetsedwa ndi makompyuta imasungunuka ndikusakaniza ufawo kukhala mawonekedwe olimba.Ikani wosanjikiza wina pamwamba pa wosanjikizawu ndikugwiritsa ntchito laser sintering.Bwerezani ndondomekoyi mpaka gawolo litatha.Ufawo umachotsedwa ku gawo lopanda laser, ndikusiya gawo losindikizidwa la 3D lomwe likufanana ndi zojambula zoyambirira za CAD.Chomalizacho chimakhala chofanana ndi njira ya microfluidic, kusiyana kwakukulu ndikuti zigawo za microfluidic nthawi zambiri zimakhala ziwiri-dimensional (flat), pogwiritsa ntchito zowonjezera zowonjezera, zovuta zowonongeka zimatha kupangidwa mu geometry yamitundu itatu.Ma faucets awa akupezeka ngati magawo osindikizidwa a 3D mu 316L chitsulo chosapanga dzimbiri ndi titaniyamu.Ma aloyi ambiri azitsulo, ma polima ndi zinthu zina zadothi zitha kugwiritsidwa ntchito popanga zida pogwiritsa ntchito njirayi ndipo zidzaganiziridwa pazopanga / zopangira zamtsogolo.
Mpunga.1. Chithunzi (a) ndi chithunzi (b) cha 90 μl Mott static chosakanizira chosonyeza gawo lodutsana la njira yamadzimadzi yosakanikirana ndi buluu.
Thamangani ma computational fluid dynamics (CFD) mafanizidwe a static mixer performance panthawi ya mapangidwe kuti athandizire kupanga mapangidwe abwino komanso kuchepetsa kuyesa kowononga nthawi komanso kokwera mtengo.CFD kayeseleledwe ka ma static mixers ndi mipope wamba (no-mixer kayeseleledwe) ntchito COMSOL Multiphysics software phukusi.Kuyerekeza pogwiritsa ntchito makina amadzimadzi a laminar oyendetsedwa ndi mphamvu kuti mumvetsetse kuthamanga kwamadzi ndi kuthamanga kwa gawo.Mphamvu yamadzimadzi iyi, yophatikizidwa ndi kayendedwe ka mankhwala amitundu yamagawo am'manja, imathandizira kumvetsetsa kusakanikirana kwamadzimadzi awiri osiyanasiyana.Chitsanzocho chimawerengedwa ngati ntchito ya nthawi, yofanana ndi masekondi a 10, kuti muwerenge mosavuta pamene mukufufuza mayankho ofanana.Deta yachidziwitso inapezedwa mu kafukufuku wokhudzana ndi nthawi pogwiritsa ntchito chida chowonetsera mfundo, pomwe mfundo yomwe ili pakati pa kutuluka idasankhidwa kuti ikhale yosonkhanitsa deta.The CFD chitsanzo ndi mayesero experimental ntchito zosungunulira awiri osiyana kudzera proportional sampuli valavu ndi kupopera dongosolo, chifukwa mu m'malo pulagi aliyense zosungunulira mu mzere zitsanzo.Zosungunulirazi zimasakanizidwa mu chosakanizira chokhazikika.Zithunzi 2 ndi 3 zikuwonetsa zofananira zoyenda kudzera mu chitoliro chokhazikika (palibe chosakanizira) komanso kudzera pa chosakaniza cha Mott static, motsatana.Kuyerekezera kunayendetsedwa pa chubu chowongoka cha 5 masentimita ndi 0.25 mm ID kuti asonyeze lingaliro la kusinthasintha mapulagi a madzi ndi acetonitrile yoyera mu chubu popanda chosakaniza chosasunthika, monga momwe tawonetsera pa Chithunzi 2. Kuyerekezera kunagwiritsa ntchito miyeso yeniyeni ya chubu ndi chosakaniza ndi kuthamanga kwa 0 .3 ml / min.
Mpunga.2. Kuyerekezera kwa CFD kutuluka mu chubu cha 5 masentimita ndi mkati mwake ndi 0.25 mm kuimira zomwe zimachitika mu chubu cha HPLC, mwachitsanzo, popanda chosakaniza.Kufiira kwathunthu kumayimira gawo lalikulu la madzi.Buluu limayimira kusowa kwa madzi, mwachitsanzo, acetonitrile yoyera.Magawo ophatikizika amatha kuwoneka pakati pa mapulagi osinthira amadzimadzi awiri osiyanasiyana.
Mpunga.3. Chosakaniza chosasunthika chokhala ndi voliyumu ya 30 ml, yopangidwa ndi phukusi la pulogalamu ya COMSOL CFD.Nthanoyi imayimira gawo lalikulu la madzi mu chosakanizira.Madzi oyera amawonetsedwa mu acetonitrile ofiira ndi oyera mu buluu.Kusintha kwa gawo lalikulu la madzi ofananirako kumayimiridwa ndi kusintha kwa mtundu wa kusakaniza kwa madzi awiri.
Pa mkuyu.4 ikuwonetsa kafukufuku wovomerezeka wa chitsanzo chogwirizanitsa pakati pa kusakaniza bwino ndi kusakaniza voliyumu.Pamene kusakaniza voliyumu ukuwonjezeka, kusakaniza bwino kudzawonjezeka.Kudziwa kwa olemba, mphamvu zina zovuta zakuthupi zomwe zimagwira mkati mwa chosakanizira sizingaganizidwe mumtundu uwu wa CFD, zomwe zimapangitsa kuti pakhale kusakanikirana kwakukulu pamayesero oyesera.The experimental kusanganikirana bwino anayesedwa ngati kuchuluka kuchepetsa m`munsi sinusoid.Kuonjezera apo, kuwonjezereka kwa msana wam'mbuyo nthawi zambiri kumabweretsa milingo yambiri yosakanikirana, yomwe siimaganiziridwa poyerekezera.
Makhalidwe otsatirawa a HPLC ndi kukhazikitsidwa kwa mayeso adagwiritsidwa ntchito kuyeza mafunde a sine yaiwisi kuti afanizire magwiridwe antchito a osakaniza osiyanasiyana osasunthika.Chithunzi cha 5 chikuwonetsa mawonekedwe amtundu wa HPLC/UHPLC.Chosakaniza chosasunthika chinayesedwa poyika chosakaniza molunjika pambuyo pa mpope komanso pamaso pa jekeseni ndi gawo lolekanitsa.Miyezo yambiri yam'mbuyo ya sinusoidal imapangidwa podutsa jekeseni ndi capillary column pakati pa static mixer ndi UV detector.Mukawunika kuchuluka kwa ma sign-to-phokoso ndi/kapena kusanthula mawonekedwe apamwamba, kasinthidwe kachitidwe kakuwoneka pa chithunzi 5.
Chithunzi 4. Chiwembu cha kusakaniza bwino ndi kusakaniza voliyumu kwa mitundu yosiyanasiyana ya static mixers.Kusadetsedwa kwamalingaliro kumatsata zomwezo monga deta yoyeserera yoyeserera yotsimikizira kutsimikizika kwa zoyeserera za CFD.
Dongosolo la HPLC lomwe linagwiritsidwa ntchito poyesa izi linali Agilent 1100 Series HPLC yokhala ndi chowunikira cha UV choyendetsedwa ndi PC yoyendetsa pulogalamu ya Chemstation.Gome 1 likuwonetsa momwe mungayesere zosakaniza zosakaniza powunika ma sinusoid muzochitika ziwiri.Mayesero oyesera anachitidwa pa zitsanzo ziwiri zosiyana za zosungunulira.Zosungunulira ziwiri zosakanikirana ngati 1 inali zosungunulira A (20 mM ammonium acetate m'madzi osungunuka) ndi zosungunulira B (80% acetonitrile (ACN) / 20% madzi opangidwa ndi deionized).Pankhani yachiwiri, zosungunulira A zinali yankho la 0.05% acetone (label) m'madzi opangidwa ndi deionized.Zosungunulira B ndi chisakanizo cha 80/20% methanol ndi madzi.Pankhani ya 1, mpopeyo inayikidwa pa mlingo wa 0.25 ml / min mpaka 1.0 ml / min, ndipo ngati 2, mpopeyo inayikidwa pa mlingo wa 1 ml / min.Pazochitika zonsezi, chiŵerengero cha kusakaniza kwa zosungunulira A ndi B chinali 20% A / 80% B. Chojambuliracho chinayikidwa ku 220 nm ngati 1, ndipo kuyamwa kwakukulu kwa acetone ngati 2 kunayikidwa kumtunda wa 265 nm.
Table 1. Kukonzekera kwa HPLC pa Milandu 1 ndi 2 Mlandu 1 Mlandu 2 Kuthamanga kwa Pampu 0.25 ml/mphindi mpaka 1.0 ml/mphindi 1.0 ml/mphindi Kusungunula A 20 mM ammonium acetate m'madzi opangidwa ndi deionized 0.05% Acetone m'madzi opangidwa ndi deionized Solvent B / 80% 80% ACN 2% Acetoni madzi acetoni (80% ACN) 20% deionized madzi zosungunulira chiŵerengero 20% A / 80% B 20% A / 80% B Detector 220 nm 265 nm
Mpunga.6. Mapulani a mafunde osakanikirana a sine amayezedwa isanayambe kapena itatha kugwiritsa ntchito fyuluta yotsika kuti achotse zigawo zoyambira zoyendetsa chizindikiro.
Chithunzi 6 ndi chitsanzo cha phokoso losakanikirana mu Mlandu Woyamba, wowonetsedwa ngati njira yobwerezabwereza ya sinusoidal yomwe imayikidwa pamwamba pa mayendedwe oyambira.Baseline drift ndi kutsika pang'onopang'ono kapena kutsika kwa siginecha yakumbuyo.Ngati dongosololi sililoledwa kuti lifanane motalika mokwanira, limagwa, koma limasuntha molakwika ngakhale dongosolo litakhala lokhazikika.Kusuntha koyambira uku kumakonda kuchulukirachulukira pamene makina akugwira ntchito motsetsereka kapena m'malo othamanga kwambiri.Izi zikachitika, zimakhala zovuta kufananiza zotsatira kuchokera ku zitsanzo kupita ku zitsanzo, zomwe zingathe kugonjetsedwa pogwiritsa ntchito fyuluta yotsika pang'onopang'ono ku deta yaiwisi kuti muchotse kusiyana kwafupipafupi, potero kumapereka chiwembu cha oscillation chokhala ndi maziko athyathyathya.Pa mkuyu.Chithunzi 6 chikuwonetsanso chiwembu cha phokoso loyambira la osakaniza atagwiritsa ntchito fyuluta yotsika.
Nditamaliza CFD kayeseleledwe ndi kuyezetsa koyamba experimenters, atatu osiyana malo amodzi mixers anali kenako anayamba ntchito zigawo zikuluzikulu zamkati tafotokozazi ndi mabuku atatu mkati: 30 µl, 60 µl ndi 90 µl.Mtunduwu umakhudza kuchuluka kwa ma voliyumu ndi magwiridwe antchito osakanikirana omwe amafunikira pakuwunika kocheperako kwa HPLC komwe kusakanikirana bwino komanso kubalalitsidwa kochepa kumafunikira kuti apange zoyambira zochepa.Pa mkuyu.7 ikuwonetsa miyeso yoyambira ya sine wave yomwe idapezedwa pamayeso a Chitsanzo 1 (acetonitrile ndi ammonium acetate ngati ma tracers) okhala ndi ma voliyumu atatu osakaniza osasunthika ndipo osayika zosakaniza.Miyezo yoyesera ya zotsatira zomwe zasonyezedwa mu Chithunzi 7 zinkachitika nthawi zonse mu mayesero onse a 4 malinga ndi ndondomeko yomwe yafotokozedwa mu Table 1 pa mlingo wosungunulira wa 0.5 ml / min.Ikani mtengo wochotsera pamagulu a data kuti athe kuwonetsedwa mbali ndi mbali popanda kuphatikizika kwa siginecha.Offset sichimakhudza matalikidwe a siginecha yomwe imagwiritsidwa ntchito kuweruza magwiridwe antchito a chosakanizira.Pafupifupi matalikidwe a sinusoidal popanda chosakanizira anali 0.221 mAi, pamene matalikidwe a static Mott mixers pa 30 µl, 60 µl, ndi 90 µl adatsikira ku 0.077, 0.017, ndi 0.004 mAi, motero.
Chithunzi 7. HPLC UV Detector Signal Offset vs. Time for Case 1 (acetonitrile yokhala ndi ammonium acetate indicator) kusonyeza zosungunulira kusakaniza popanda chosakanizira, 30 µl, 60 µl ndi 90 µl Mott mixers kusonyeza kusakaniza bwino (pansi chizindikiro matalikidwe) monga static kusakaniza voliyumu kumawonjezera.(zochotsa zenizeni zenizeni: 0.13 (palibe chosakanizira), 0.32, 0.4, 0.45mA kuti ziwonetsedwe bwino).
Deta yomwe ikuwonetsedwa mkuyu.8 ndizofanana ndi mkuyu 7, koma nthawi ino zikuphatikiza zotsatira za osakaniza atatu omwe amagwiritsidwa ntchito kwambiri a HPLC okhala ndi ma voliyumu amkati a 50 µl, 150 µl ndi 250 µl.Mpunga.Chithunzi 8. HPLC UV Detector Signal Offset motsutsana ndi Time Plot for Case 1 (Acetonitrile ndi Ammonium Acetate monga zizindikiro) kusonyeza kusanganikirana kwa zosungunulira popanda static mixer, mndandanda watsopano wa Mott static mixers, ndi osakaniza atatu osakanikirana (zowonongeka zenizeni za deta ndi 0.1 (popanda chosakaniza), 0.60.82, 0.60.82, 0.60. 9 mA motsatana kuti aziwonetsa bwino).Kuchepetsa kwa maperesenti a maziko a sine wave kumawerengedwa ndi chiŵerengero cha matalikidwe a sine wave mpaka matalikidwe popanda chosakanizira choikidwa.Maperesenti a sine wave attenuation pa Milandu 1 ndi 2 alembedwa mu Table 2, pamodzi ndi ma voliyumu amkati a chosakanizira chatsopano chosasunthika ndi zosakaniza zisanu ndi ziwiri zomwe zimagwiritsidwa ntchito kwambiri pamakampani.Zomwe zili mu Zithunzi 8 ndi 9, komanso zowerengera zomwe zaperekedwa mu Table 2, zikuwonetsa kuti Mott Static Mixer ikhoza kupereka mpaka 98.1% sine wave attenuation, kupitirira kwambiri ntchito ya chosakanizira wamba HPLC pansi paziyeso izi.Chithunzi 9. HPLC UV detector signal offset against time plot for case 2 (methanol ndi acetone as tracers) kusonyeza kuti palibe chosakanizira chokhazikika (chophatikizidwa), mndandanda watsopano wa osakaniza a Mott static ndi osakaniza awiri ochiritsira (zowonongeka zenizeni za deta ndi 0, 11 (popanda chosakanizira. ), 0.22, 0.5 mA kwa kuwonetsera bwino.Zosakaniza zisanu ndi ziwiri zomwe zimagwiritsidwa ntchito kwambiri pamakampani zidawunikidwanso.Izi zikuphatikiza zosakaniza zokhala ndi mavoliyumu atatu osiyanasiyana amkati kuchokera ku kampani A (yosankhidwa Yosakaniza A1, A2 ndi A3) ndi kampani B (yosankhidwa Yosakaniza B1, B2 ndi B3).Kampani C idavotera kukula kumodzi kokha.
Table 2. Static Mixer Stirring Characterities ndi Internal Volume Static Mixer Case 1 Sinusoidal Recovery: Acetonitrile Test (Kuchita Bwino) Mlandu 2 Sinusoidal Recovery: Methanol Water Test (Kuchita Bwino) Volume Yamkati (µl) Palibe Chosakaniza - - 0 Mott% 6% 69 30 Mott. 1.3% 60 Mott 90 98.1% 97.5% 90 Mixer A1 66.4% 73.7% 50 Mixer A2 89.8% 91.6% 150 Mixer A3 92.2% 94.5% 250 Mixer 250% B1 45.8% B1 44.8% B1 44.8%. 370 Wosakaniza C 97.2% 97.4% 250
Kuwunika kwa zotsatira mu Chithunzi 8 ndi Table 2 kukuwonetsa kuti chosakaniza cha 30 µl Mott static chimakhala ndi kusakaniza kofanana ndi kosakaniza kwa A1, mwachitsanzo 50 µl, komabe, 30 µl Mott ili ndi 30% kuchepera kwa voliyumu yamkati.Poyerekeza 60 µl Mott chosakanizira ndi 150 µl mkati voliyumu A2 chosakanizira, panali kusintha pang'ono pakusakaniza bwino kwa 92% motsutsana ndi 89%, koma chofunika kwambiri, kusakaniza kwakukulu kumeneku kunakwaniritsidwa pa 1/3 ya voliyumu yosakaniza.chosakaniza chofananira A2.Kuchita kwa chosakanizira cha 90 µl Mott kunkatsatira zomwezo monga chosakanizira cha A3 chokhala ndi voliyumu yamkati ya 250 µl.Kupititsa patsogolo kusakaniza kwa 98% ndi 92% kunawonedwanso ndi kuchepetsedwa kwa 3-kuchepetsa voliyumu yamkati.Zotsatira zofanana ndi zofananitsa zinapezedwa kwa osakaniza B ndi C. Zotsatira zake, mndandanda watsopano wa osakaniza static Mott PerfectPeak TM umapereka kusakaniza kwapamwamba kusiyana ndi osakaniza opikisana nawo, koma ndi mawu ocheperapo amkati, opereka phokoso lakumbuyo labwino komanso chiŵerengero chabwino cha chizindikiro-ku-phokoso, Kusanthula kwabwinoko, mawonekedwe apamwamba ndi chigamulo chapamwamba.Zofananira zosakanikirana bwino zidawonedwa m'maphunziro onse a Mlandu 1 ndi Mlandu wa 2.Pa Mlandu 2, mayesero anachitidwa pogwiritsa ntchito (methanol ndi acetone monga zizindikiro) kuyerekeza kusakaniza bwino kwa 60 ml Mott, chosakanizira chofananira A1 (voliyumu yamkati 50 µl) ndi chosakaniza chofananira B1 (voliyumu yamkati 35 µl)., magwiridwe antchito anali osauka popanda chosakanizira choyikidwa, koma chimagwiritsidwa ntchito pofufuza zoyambira.Chosakaniza cha 60 ml cha Mott chinatsimikizira kukhala chosakaniza bwino kwambiri mu gulu loyesera, kupereka kuwonjezeka kwa 90% pakusakaniza bwino.Wosakaniza wofananira A1 adawona kusintha kwa 75% pakusakaniza bwino ndikutsatiridwa ndi kusintha kwa 45% mu chosakaniza chofananira cha B1.Mayeso oyambira ochepetsa mafunde a sine ndi kuchuluka kwa mafunde adachitika pazosakaniza zingapo pansi pamikhalidwe yofanana ndi mayeso a sine curve mu Mlandu 1, ndikungosintha koyenda.Detayo idawonetsa kuti pamayendedwe oyenda kuchokera ku 0,25 mpaka 1 ml / min, kuchepa koyambirira kwa sine wave kunakhalabe kosasintha pama voliyumu onse atatu osakaniza.Kwa osakaniza awiri ang'onoang'ono a voliyumu, pali kuwonjezeka pang'ono kwa sinusoidal contraction pamene kuthamanga kwa magazi kumachepa, zomwe zimayembekezereka chifukwa cha kuwonjezeka kwa nthawi yokhala ndi zosungunulira mu chosakaniza, zomwe zimapangitsa kuti kusakanikirana kuchuluke.Kuchotsa kwa sine wave kumayembekezeredwa kuwonjezeka pamene kutuluka kumacheperachepera.Komabe, pa voliyumu yayikulu kwambiri yosakanikirana yokhala ndi ma sine wave base base attenuation, sine wave base attenuation idakhalabe yosasinthika (m'kati mwa kusatsimikizika koyesera), zoyambira kuyambira 95% mpaka 98%.Mpunga.10. Kuchepetsa kwakukulu kwa sine wave motsutsana ndi mlingo wothamanga ngati 1. Kuyesedwa kunkachitidwa pansi pamikhalidwe yofanana ndi mayeso a sine ndi kusintha kwa kayendedwe ka kayendedwe kake, jekeseni 80% ya 80 / 20 osakaniza acetonitrile ndi madzi ndi 20% ya 20 mM ammonium acetate.
Mitundu yomwe yangopangidwa kumene ya PerfectPeak TM inline static mixers yokhala ndi ma voliyumu atatu amkati: 30 µl, 60 µl ndi 90 µl imakwirira voliyumu ndi kusakanikirana kwa magwiridwe antchito omwe amafunikira pakuwunika kwa HPLC komwe kumafunikira kusakanikirana bwino komanso kutsika kobalalika.Chosakaniza chatsopano cha static chimakwaniritsa izi pogwiritsa ntchito ukadaulo watsopano wosindikiza wa 3D kuti apange mawonekedwe apadera a 3D omwe amapereka kusanganikirana kwamphamvu kwa hydrodynamic static ndikuchepetsa kwambiri phokoso lapansi pa voliyumu yosakanikirana yamkati.Kugwiritsa ntchito 1/3 ya voliyumu yamkati ya chosakaniza wamba kumachepetsa phokoso loyambira ndi 98%.Amenewa mixers zigwirizana interconnected atatu azithunzithunzi otaya ngalande ndi osiyana mtanda-gawo madera osiyana njira utali ngati madzi mitanda zovuta zojambula zotchinga mkati.Banja latsopano la osakaniza osasunthika limapereka ntchito yabwino kuposa osakaniza opikisana, koma ndi mawu ochepa amkati, zomwe zimapangitsa kuti phokoso likhale lomveka bwino komanso kuti likhale ndi malire ochepetsera, komanso kupititsa patsogolo mawonekedwe apamwamba, kuchita bwino komanso kuthetsa kukhudzidwa kwakukulu.
M'magazini iyi Chromatography - RP-HPLC yochezeka ndi chilengedwe - Kugwiritsa ntchito chromatography yachipolopolo m'malo mwa acetonitrile ndi isopropanol posanthula ndi kuyeretsa - Chromatograph yatsopano ya gasi ya…
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