Ib lub tshuab sib tov inline static tshiab tau tsim tshwj xeeb los ua kom tau raws li qhov yuav tsum tau ua ntawm cov kua chromatography siab (HPLC) thiab cov kua chromatography siab heev (HPLC thiab UHPLC). Kev sib tov tsis zoo ntawm ob lossis ntau theem mobile tuaj yeem ua rau muaj qhov sib piv ntawm lub teeb liab thiab suab nrov dua, uas txo qhov rhiab heev. Kev sib tov sib xyaw zoo ntawm ob lossis ntau cov kua nrog qhov ntim sab hauv tsawg kawg nkaus thiab qhov loj ntawm lub tshuab sib tov static sawv cev rau tus qauv siab tshaj plaws ntawm lub tshuab sib tov static zoo tagnrho. Lub tshuab sib tov static tshiab ua tiav qhov no los ntawm kev siv cov thev naus laus zis luam ntawv 3D tshiab los tsim cov qauv 3D tshwj xeeb uas muab kev sib tov hydrodynamic zoo dua nrog qhov feem pua ​​​​​​tsawg tshaj plaws hauv cov nthwv dej sine ib chav tsev ntim sab hauv ntawm cov khoom sib tov. Siv 1/3 ntawm qhov ntim sab hauv ntawm lub tshuab sib tov ib txwm txo cov nthwv dej sine yooj yim los ntawm 98%. Lub tshuab sib tov muaj cov channel ntws 3D sib txuas nrog ntau thaj chaw hla thiab qhov ntev ntawm txoj kev thaum cov kua hla cov geometries 3D nyuaj. Kev sib tov raws ntau txoj kev ntws uas tig mus los, ua ke nrog kev sib hloov hauv zos thiab kev hloov pauv, ua rau muaj kev sib tov ntawm qhov ntsuas micro, meso thiab macro. Lub tshuab sib tov tshwj xeeb no yog tsim los ntawm kev siv cov qauv kev ua lej (CFD) kev sim. Cov ntaub ntawv sim uas tau nthuav tawm qhia tias kev sib tov zoo heev tau ua tiav nrog qhov ntim sab hauv tsawg kawg nkaus.
Tau ntau tshaj 30 xyoo, cov kua chromatography tau siv rau hauv ntau yam lag luam, suav nrog cov tshuaj, cov tshuaj tua kab, kev tiv thaiv ib puag ncig, kev tshawb fawb txog kev ua txhaum cai, thiab kev tshuaj xyuas tshuaj lom neeg. Lub peev xwm los ntsuas rau ib feem ib lab lossis tsawg dua yog qhov tseem ceeb rau kev txhim kho thev naus laus zis hauv txhua yam lag luam. Kev sib xyaw ua haujlwm tsis zoo ua rau muaj qhov sib piv tsis zoo ntawm lub teeb liab-rau-suab nrov, uas yog kev ntxhov siab rau lub zej zog chromatography hauv cov nqe lus ntawm kev ntes thiab kev rhiab heev. Thaum sib xyaw ob lub HPLC cov kuab tshuaj, qee zaum nws yuav tsum tau yuam kom sib xyaw los ntawm sab nraud kom sib xyaw ob lub kuab tshuaj vim tias qee cov kuab tshuaj tsis sib xyaw zoo. Yog tias cov kuab tshuaj tsis sib xyaw kom huv si, kev puas tsuaj ntawm HPLC chromatogram yuav tshwm sim, qhia nws tus kheej ua suab nrov dhau thiab / lossis cov duab tsis zoo. Nrog kev sib xyaw tsis zoo, suab nrov dhau yuav tshwm sim ua nthwv dej sine (nce thiab poob) ntawm lub teeb liab ntes dhau sijhawm. Tib lub sijhawm, kev sib xyaw tsis zoo tuaj yeem ua rau cov ncov dav thiab asymmetric, txo qis kev ua haujlwm analytical, cov duab siab tshaj plaws, thiab kev daws teeb meem siab tshaj plaws. Kev lag luam tau lees paub tias cov khoom sib xyaw hauv-kab thiab tee static yog ib txoj hauv kev los txhim kho cov kev txwv no thiab tso cai rau cov neeg siv kom ua tiav cov kev txwv qis dua (kev rhiab heev). Lub tshuab sib tov zoo tagnrho muaj cov txiaj ntsig ntawm kev sib tov zoo, qhov ntim tsawg thiab qhov siab poob qis nrog qhov ntim tsawg kawg nkaus thiab qhov siab tshaj plaws ntawm lub kaw lus. Tsis tas li ntawd, thaum kev tshuaj xyuas nyuaj dua, cov kws tshuaj xyuas yuav tsum siv cov tshuaj polar thiab cov tshuaj uas nyuaj rau sib tov tas li. Qhov no txhais tau tias kev sib tov zoo dua yog qhov yuav tsum muaj rau kev sim yav tom ntej, ua rau muaj kev xav tau tsim thiab kev ua haujlwm zoo dua ntawm lub tshuab sib tov.
Mott nyuam qhuav tsim cov khoom sib tov PerfectPeakTM inline static tshiab uas muaj peb lub ntim sab hauv: 30 µl, 60 µl thiab 90 µl. Cov qhov loj no npog ntau yam ntim thiab cov yam ntxwv sib tov uas xav tau rau feem ntau HPLC qhov twg xav tau kev sib tov zoo dua thiab kev sib xyaw tsawg. Tag nrho peb tus qauv yog 0.5″ hauv txoj kab uas hla thiab xa cov kev ua tau zoo tshaj plaws hauv kev lag luam hauv kev tsim me me. Lawv yog ua los ntawm 316L stainless hlau, passivated rau inertness, tab sis titanium thiab lwm yam corrosion resistant thiab chemically inert hlau alloys kuj muaj. Cov khoom sib tov no muaj lub siab ua haujlwm siab tshaj plaws txog li 20,000 psi. Hauv daim duab 1a yog daim duab ntawm 60 µl Mott static mixer tsim los muab kev sib tov zoo tshaj plaws thaum siv lub ntim sab hauv me dua li cov khoom sib tov txheem ntawm hom no. Qhov kev tsim khoom sib tov tshiab no siv cov thev naus laus zis tshiab los tsim cov qauv 3D tshwj xeeb uas siv cov dej ntws sab hauv tsawg dua li cov khoom sib tov tam sim no siv hauv kev lag luam chromatography kom ua tiav kev sib tov zoo. Cov khoom sib tov no muaj cov kav dej ntws peb-seem uas sib txuas nrog cov cheeb tsam sib txawv thiab cov kab ntev sib txawv thaum cov kua hla cov teeb meem geometric nyuaj sab hauv. Hauv daim duab 1b qhia txog daim duab qhia txog cov khoom sib tov tshiab, uas siv cov khoom siv sib xyaw HPLC 10-32 rau kev nkag thiab tawm, thiab muaj cov ciam teb xiav ntawm qhov chaw sib tov sab hauv uas tau txais patent. Cov cheeb tsam sib txawv ntawm cov kab ntws sab hauv thiab kev hloov pauv ntawm kev coj ntws hauv qhov ntim ntws sab hauv tsim cov cheeb tsam ntawm kev ntws turbulent thiab laminar, ua rau muaj kev sib tov ntawm micro, meso thiab macro. Kev tsim cov khoom sib tov tshwj xeeb no siv cov qauv siv computational fluid dynamics (CFD) los tshuaj xyuas cov qauv ntws thiab txhim kho qhov kev tsim ua ntej prototyping rau kev sim tshuaj ntsuam hauv tsev thiab kev soj ntsuam cov neeg siv khoom. Kev tsim khoom ntxiv yog cov txheej txheem ntawm kev luam cov khoom geometric 3D ncaj qha los ntawm cov duab kos CAD yam tsis tas yuav tsum tau siv cov tshuab ib txwm muaj (cov tshuab milling, lathes, thiab lwm yam). Cov khoom sib tov static tshiab no tau tsim los tsim siv cov txheej txheem no, qhov twg lub cev sib tov tau tsim los ntawm cov duab kos CAD thiab cov khoom tau tsim (luam tawm) txheej los ntawm txheej siv kev tsim khoom ntxiv. Ntawm no, ib txheej hmoov hlau uas muaj li ntawm 20 microns tuab raug tso rau hauv, thiab lub laser uas tswj los ntawm lub computer xaiv cov hmoov yaj thiab sib xyaw ua ke rau hauv ib daim ntawv khov kho. Siv lwm txheej rau saum txheej no thiab siv laser sintering. Rov ua qhov txheej txheem no kom txog thaum qhov khoom tiav tag nrho. Tom qab ntawd cov hmoov raug tshem tawm ntawm qhov tsis yog laser bonded, tawm hauv ib feem luam tawm 3D uas phim cov duab kos CAD thawj. Cov khoom kawg zoo ib yam li cov txheej txheem microfluidic, nrog rau qhov sib txawv tseem ceeb yog tias cov khoom microfluidic feem ntau yog ob-seem (tiaj tus), thaum siv cov khoom siv ntxiv, cov qauv ntws nyuaj tuaj yeem tsim tau hauv peb-seem geometry. Cov kais dej no tam sim no muaj nyob rau hauv 3D luam tawm qhov chaw hauv 316L stainless hlau thiab titanium. Feem ntau cov hlau alloys, polymers thiab qee cov ceramics tuaj yeem siv los ua cov khoom siv siv txoj kev no thiab yuav raug txiav txim siab hauv cov qauv tsim / khoom yav tom ntej.
Mov. 1. Duab (a) thiab daim duab qhia (b) ntawm 90 μl Mott static mixer uas qhia txog qhov seem ntawm txoj kev ntws ntawm cov kua dej sib tov uas muaj xim xiav.
Khiav kev siv computer fluid dynamics (CFD) simulations ntawm kev ua haujlwm ntawm lub static mixer thaum lub sijhawm tsim qauv los pab tsim cov qauv tsim zoo thiab txo cov kev sim thiab kev ua yuam kev uas siv sijhawm ntau thiab kim. CFD simulation ntawm cov static mixers thiab cov kav dej txheem (tsis muaj mixer simulation) siv COMSOL Multiphysics software pob. Kev ua qauv siv lub zog siab-tsav laminar fluid mechanics kom nkag siab txog qhov ceev ntawm cov kua thiab siab hauv ib feem. Cov kua dynamics no, ua ke nrog kev thauj mus los ntawm cov tshuaj lom neeg ntawm cov khoom sib xyaw theem txawb, pab nkag siab txog kev sib xyaw ntawm ob lub kua sib xyaw sib txawv. Tus qauv no tau kawm ua lub luag haujlwm ntawm lub sijhawm, sib npaug rau 10 vib nas this, rau kev yooj yim ntawm kev xam thaum nrhiav cov kev daws teeb meem sib piv. Cov ntaub ntawv theoretical tau txais hauv kev tshawb fawb txog lub sijhawm siv cov cuab yeej projection probe, qhov twg ib qho taw tes hauv nruab nrab ntawm qhov tawm tau xaiv rau kev sau cov ntaub ntawv. Tus qauv CFD thiab kev sim sim siv ob lub tshuaj sib txawv los ntawm lub qhov ntsuas piv thiab lub kaw lus twj tso kua mis, ua rau muaj lub qhov hloov pauv rau txhua lub tshuaj hauv kab kuaj. Cov tshuaj no tom qab ntawd sib xyaw hauv lub tshuaj sib xyaw zoo li qub. Cov Duab 2 thiab 3 qhia txog kev ua piv txwv ntawm kev ntws los ntawm cov yeeb nkab txheem (tsis muaj lub tshuab sib tov) thiab los ntawm Mott lub tshuab sib tov zoo li qub, raws li. Kev ua piv txwv tau khiav ntawm ib lub raj ncaj 5 cm ntev thiab 0.25 hli ID los qhia txog lub tswv yim ntawm kev hloov pauv cov ntsaws dej thiab acetonitrile ntshiab rau hauv lub raj thaum tsis muaj lub tshuab sib tov zoo li qub, raws li pom hauv Daim Duab 2. Kev ua piv txwv siv qhov ntev ntawm lub raj thiab lub tshuab sib tov thiab tus nqi ntws ntawm 0.3 ml/min.
Mov. 2. Kev ua qauv ntawm CFD ntws hauv lub raj 5 cm nrog lub cheeb sab hauv ntawm 0.25 hli los sawv cev rau qhov tshwm sim hauv lub raj HPLC, piv txwv li thaum tsis muaj lub tshuab sib tov. Xim liab tag nrho sawv cev rau qhov loj ntawm cov dej. Xim xiav sawv cev rau qhov tsis muaj dej, piv txwv li acetonitrile ntshiab. Cov cheeb tsam sib kis tuaj yeem pom ntawm cov ntsaws sib hloov ntawm ob lub kua sib txawv.
Mov. 3. Lub tshuab sib tov uas muaj qhov ntim ntawm 30 ml, ua qauv hauv COMSOL CFD software pob. Cov lus piav qhia sawv cev rau feem pua ​​ntawm cov dej hauv lub tshuab sib tov. Dej ntshiab yog qhia ua xim liab thiab acetonitrile ntshiab yog xim xiav. Qhov kev hloov pauv ntawm feem pua ​​ntawm cov dej ua qauv yog sawv cev los ntawm kev hloov pauv ntawm cov xim ntawm kev sib tov ntawm ob lub kua.
Hauv daim duab 4 qhia txog kev tshawb nrhiav txog kev sib raug zoo ntawm tus qauv sib xyaw ua ke thiab qhov ntim ntawm kev sib tov. Thaum qhov ntim ntawm kev sib tov nce ntxiv, qhov kev sib tov zoo yuav nce ntxiv. Raws li cov kws sau ntawv paub, lwm yam zog nyuaj uas ua haujlwm hauv lub tshuab sib tov tsis tuaj yeem suav nrog hauv tus qauv CFD no, ua rau muaj kev sib tov zoo dua hauv kev sim. Qhov kev sib tov zoo ntawm kev sim tau ntsuas raws li feem pua ​​​​​​ntawm kev txo qis hauv lub hauv paus sinusoid. Tsis tas li ntawd, qhov siab rov qab nce ntxiv feem ntau ua rau muaj qib sib tov siab dua, uas tsis tau coj los xav hauv kev sim.
Cov xwm txheej HPLC thiab kev teeb tsa kev sim hauv qab no tau siv los ntsuas cov nthwv dej sine raw los sib piv cov kev ua tau zoo ntawm cov sib tov sib txawv. Daim duab hauv Daim Duab 5 qhia txog qhov kev teeb tsa HPLC/UHPLC system ib txwm muaj. Lub sib tov sib xws tau sim los ntawm kev tso lub sib tov ncaj qha tom qab lub twj tso kua mis thiab ua ntej lub tshuab txhaj tshuaj thiab kem sib cais. Feem ntau cov kev ntsuas sinusoidal keeb kwm yav dhau los yog ua los ntawm kev hla lub tshuab txhaj tshuaj thiab kem capillary ntawm lub sib tov sib xws thiab lub tshuab ntes UV. Thaum ntsuas qhov sib piv ntawm lub teeb liab thiab / lossis kev tshuaj xyuas lub ncov, qhov kev teeb tsa ntawm lub kaw lus tau pom hauv Daim Duab 5.
Daim Duab 4. Daim duab qhia txog kev sib tov zoo piv rau qhov ntim sib tov rau ntau yam khoom sib tov zoo li qub. Qhov tsis huv ntawm lub tswv yim ua raws li qhov sib txawv ntawm cov ntaub ntawv tsis huv ntawm kev sim uas lees paub qhov tseeb ntawm CFD kev sim.
Lub tshuab HPLC siv rau qhov kev sim no yog Agilent 1100 Series HPLC nrog lub tshuab ntes UV tswj los ntawm PC khiav Chemstation software. Rooj 1 qhia txog cov xwm txheej tuning ib txwm rau kev ntsuas qhov ua tau zoo ntawm cov khoom sib tov los ntawm kev saib xyuas cov sinusoids yooj yim hauv ob qhov kev tshawb fawb. Kev sim sim tau ua tiav rau ob qho piv txwv sib txawv ntawm cov kuab tshuaj. Ob lub kuab tshuaj sib xyaw hauv rooj plaub 1 yog cov kuab tshuaj A (20 mM ammonium acetate hauv dej deionized) thiab cov kuab tshuaj B (80% acetonitrile (ACN) / 20% dej deionized). Hauv Rooj Plaub 2, cov kuab tshuaj A yog cov kua ntawm 0.05% acetone (daim ntawv lo) hauv dej deionized. Cov kuab tshuaj B yog cov sib xyaw ntawm 80/20% methanol thiab dej. Hauv rooj plaub 1, lub twj tso kua mis tau teeb tsa rau tus nqi ntws ntawm 0.25 ml / feeb txog 1.0 ml / feeb, thiab hauv rooj plaub 2, lub twj tso kua mis tau teeb tsa rau tus nqi ntws tas li ntawm 1 ml / feeb. Hauv ob qho tib si, qhov sib piv ntawm cov tshuaj sib xyaw A thiab B yog 20% ​​A/80% B. Lub tshuab ntes tau teeb tsa rau 220 nm hauv rooj plaub 1, thiab qhov nqus tau ntau tshaj plaws ntawm acetone hauv rooj plaub 2 tau teeb tsa rau qhov ntev ntawm 265 nm.
Rooj 1. HPLC Kev Teeb tsa rau Cov Ntaub Ntawv 1 thiab 2 Cov Ntaub Ntawv 1 Cov Ntaub Ntawv 2 Twj Tso Kua Roj Ceev ​​0.25 ml/min txog 1.0 ml/min 1.0 ml/min Cov kuab tshuaj A 20 mM ammonium acetate hauv dej deionized 0.05% Acetone hauv dej deionized Cov kuab tshuaj B 80% Acetonitrile (ACN) / 20% dej deionized 80% methanol / 20% dej deionized Qhov piv ntawm cov kuab tshuaj 20% A / 80% B 20% A / 80% B Cov cuab yeej ntes 220 nm 265 nm
Mov. 6. Cov duab ntawm cov nthwv dej sine sib xyaw ua ntej thiab tom qab siv cov lim dej qis-pass kom tshem tawm cov khoom sib txawv ntawm lub teeb liab.
Daim Duab 6 yog ib qho piv txwv ntawm cov suab nrov sib xyaw hauv Case 1, uas tau qhia ua tus qauv sinusoidal rov ua dua uas muaj nyob rau ntawm qhov sib txawv ntawm qhov sib txawv. Qhov sib txawv ntawm qhov sib txawv yog qhov nce qeeb lossis txo qis ntawm lub cim keeb kwm yav dhau. Yog tias lub kaw lus tsis raug tso cai kom sib npaug ntev txaus, nws feem ntau yuav poob, tab sis yuav sib txawv txawm tias lub kaw lus ruaj khov tag nrho. Qhov kev sib txawv ntawm qhov sib txawv no feem ntau nce ntxiv thaum lub kaw lus ua haujlwm hauv qhov sib txawv siab lossis qhov siab rov qab siab. Thaum qhov kev sib txawv ntawm qhov sib txawv no muaj, nws tuaj yeem nyuaj rau kev sib piv cov txiaj ntsig los ntawm cov qauv mus rau cov qauv, uas tuaj yeem kov yeej los ntawm kev siv cov lim dej qis-pass rau cov ntaub ntawv raw los lim tawm cov kev hloov pauv qis no, yog li muab cov phiaj xwm oscillation nrog lub hauv paus tiaj tus. Hauv daim duab. Daim Duab 6 kuj qhia txog daim duab ntawm lub suab nrov ntawm lub hauv paus ntawm lub mixer tom qab siv cov lim dej qis-pass.
Tom qab ua tiav CFD simulations thiab kev sim thawj zaug, peb lub tshuab sib tov sib cais tau tsim los ntawm kev siv cov khoom sab hauv uas tau piav qhia saum toj no nrog peb lub ntim sab hauv: 30 µl, 60 µl thiab 90 µl. Qhov ntau no suav nrog ntau yam ntim thiab kev sib tov ua tau zoo rau cov ntawv thov HPLC analyte qis uas xav tau kev sib tov zoo dua thiab kev sib xyaw tsawg kom tsim cov kab ke qis. Hauv daim duab 7 qhia txog kev ntsuas nthwv dej sine yooj yim tau txais ntawm lub kaw lus sim ntawm Piv txwv 1 (acetonitrile thiab ammonium acetate ua cov cim qhia) nrog peb lub ntim ntawm cov tshuab sib tov zoo li qub thiab tsis muaj cov tshuab sib tov ntsia. Cov xwm txheej sim rau cov txiaj ntsig tau pom hauv Daim Duab 7 tau tuav ruaj khov thoob plaws tag nrho 4 qhov kev sim raws li cov txheej txheem tau teev tseg hauv Rooj 1 ntawm tus nqi ntws ntawm cov kuab tshuaj ntawm 0.5 ml / min. Siv tus nqi offset rau cov ntaub ntawv teeb tsa kom lawv tuaj yeem tso tawm ib sab ntawm ib sab yam tsis muaj teeb liab sib tshooj. Offset tsis cuam tshuam rau qhov loj ntawm lub teeb liab siv los txiav txim siab qib kev ua tau zoo ntawm lub tshuab sib tov. Qhov nruab nrab sinusoidal amplitude tsis muaj lub mixer yog 0.221 mAi, thaum lub amplitudes ntawm static Mott mixers ntawm 30 µl, 60 µl, thiab 90 µl poob rau 0.077, 0.017, thiab 0.004 mAi, raws li.
Daim Duab 7. HPLC UV Detector Signal Offset vs. Lub Sijhawm rau Case 1 (acetonitrile nrog ammonium acetate indicator) qhia txog kev sib tov cov kuab tshuaj yam tsis muaj mixer, 30 µl, 60 µl thiab 90 µl Mott mixers qhia txog kev sib tov zoo dua (qhov amplitude qis dua) thaum lub ntim ntawm lub static mixer nce ntxiv. (cov ntaub ntawv tiag tiag offsets: 0.13 (tsis muaj mixer), 0.32, 0.4, 0.45mA rau kev tso saib zoo dua).
Cov ntaub ntawv qhia hauv daim duab 8 zoo ib yam li hauv daim duab 7, tab sis lub sijhawm no lawv suav nrog cov txiaj ntsig ntawm peb lub HPLC static mixers uas siv ntau nrog cov ntim sab hauv ntawm 50 µl, 150 µl thiab 250 µl. Mov. Daim duab 8. HPLC UV Detector Signal Offset piv rau Time Plot rau Case 1 (Acetonitrile thiab Ammonium Acetate ua cov cim qhia) qhia txog kev sib xyaw ntawm cov kuab tshuaj yam tsis muaj static mixer, cov koob tshiab ntawm Mott static mixers, thiab peb lub mixers ib txwm muaj (cov ntaub ntawv tiag tiag offset yog 0.1 (tsis muaj mixer), 0.32, 0.48, 0.6, 0.7, 0.8, 0.9 mA feem rau qhov zoo dua ntawm kev tso saib). Qhov feem pua ​​​​​​ntawm kev txo qis ntawm lub hauv paus sine nthwv dej yog xam los ntawm qhov sib piv ntawm qhov loj ntawm lub sine nthwv dej rau qhov loj yam tsis muaj lub mixer ntsia. Cov feem pua ​​​​​​ntawm kev txo qis ntawm sine wave rau Cases 1 thiab 2 tau teev nyob rau hauv Rooj 2, nrog rau cov ntim sab hauv ntawm lub tshuab sib tov static tshiab thiab xya lub tshuab sib tov txheem uas feem ntau siv hauv kev lag luam. Cov ntaub ntawv hauv Daim Duab 8 thiab 9, nrog rau cov kev xam pom tau nthuav tawm hauv Rooj 2, qhia tias Mott Static Mixer tuaj yeem muab txog li 98.1% sine wave attenuation, ntau dua li qhov ua tau zoo ntawm lub tshuab sib tov HPLC ib txwm muaj nyob rau hauv cov xwm txheej xeem no. Daim Duab 9. HPLC UV detector teeb liab offset piv rau lub sijhawm phiaj rau rooj 2 (methanol thiab acetone ua tracers) qhia tsis muaj static mixer (ua ke), ib qho tshiab ntawm Mott static mixers thiab ob lub tshuab sib tov ib txwm muaj (cov ntaub ntawv offsets tiag tiag yog 0, 11 (tsis muaj mixer.), 0.22, 0.3, 0.35 mA thiab rau kev tso saib zoo dua). Xya lub tshuab sib tov feem ntau siv hauv kev lag luam kuj tau raug soj ntsuam. Cov no suav nrog cov khoom sib tov nrog peb qhov ntim sab hauv sib txawv los ntawm lub tuam txhab A (xaiv Mixer A1, A2 thiab A3) thiab lub tuam txhab B (xaiv Mixer B1, B2 thiab B3). Lub Tuam Txhab C tsuas yog ntsuas ib qhov loj xwb.
Rooj 2. Cov yam ntxwv ntawm kev sib tov khoom zoo li qub thiab qhov ntim sab hauv Lub tshuab sib tov khoom zoo li qub Case 1 Sinusoidal Recovery: Acetonitrile Test (Kev ua haujlwm zoo) Case 2 Sinusoidal Recovery: Methanol Water Test (Kev ua haujlwm zoo) Internal Volume (µl) Tsis muaj Mixer – - 0 Mott 30 65% 67.2% 30 Mott 60 92.2% 91.3% 60 Mott 90 98.1% 97.5% 90 Mixer A1 66.4% 73.7% 50 Mixer A2 89.8% 91.6% 150 Mixer A3 92.2% 94.5% 250 Mixer B1 44.8% 45.7% 9 35 Mixer B2 845.% 96.2% 370 Mixer C 97.2% 97.4% 250
Kev tshuaj xyuas cov txiaj ntsig hauv Daim Duab 8 thiab Rooj 2 qhia tau hais tias 30 µl Mott static mixer muaj qhov sib xyaw zoo ib yam li A1 mixer, piv txwv li 50 µl, txawm li cas los xij, 30 µl Mott muaj 30% tsawg dua sab hauv. Thaum piv rau 60 µl Mott mixer nrog 150 µl sab hauv A2 mixer, muaj kev txhim kho me ntsis hauv kev sib xyaw ua haujlwm ntawm 92% piv rau 89%, tab sis qhov tseem ceeb dua, qib siab dua ntawm kev sib xyaw tau ua tiav ntawm 1/3 ntawm qhov ntim sib xyaw. Zoo sib xws A2. Kev ua tau zoo ntawm 90 µl Mott mixer ua raws li qhov sib xws li A3 mixer nrog qhov ntim sab hauv ntawm 250 µl. Kev txhim kho hauv kev sib xyaw ua haujlwm ntawm 98% thiab 92% kuj tau pom nrog 3-zaug txo qis hauv qhov ntim sab hauv. Cov txiaj ntsig zoo sib xws thiab kev sib piv tau txais rau cov khoom sib tov B thiab C. Yog li ntawd, cov koob tshiab ntawm cov khoom sib tov zoo li qub Mott PerfectPeakTM muab kev sib tov zoo dua li cov khoom sib tov sib piv, tab sis nrog lub ntim sab hauv tsawg dua, muab lub suab nrov zoo dua thiab lub teeb liab-rau-suab nrov zoo dua, qhov rhiab heev zoo dua Analyte, lub ncov zoo dua thiab kev daws teeb meem siab tshaj plaws. Cov qauv zoo sib xws hauv kev sib tov tau pom hauv ob qho kev tshawb fawb Case 1 thiab Case 2. Rau Case 2, kev sim tau ua tiav siv (methanol thiab acetone ua cov cim qhia) los sib piv kev sib tov ntawm 60 ml Mott, lub khoom sib tov sib piv A1 (ntim sab hauv 50 µl) thiab lub khoom sib tov sib piv B1 (ntim sab hauv 35 µl). , kev ua tau zoo tsis muaj lub khoom sib tov ntsia, tab sis nws tau siv rau kev tshuaj xyuas pib. Lub khoom sib tov 60 ml Mott tau ua pov thawj tias yog lub khoom sib tov zoo tshaj plaws hauv pawg sim, muab 90% kev nce hauv kev sib tov zoo. Lub khoom sib tov sib piv A1 pom tau 75% kev txhim kho hauv kev sib tov ua raws li 45% kev txhim kho hauv lub khoom sib tov B1 sib piv. Ib qho kev sim txo qis sine nthwv dej yooj yim nrog tus nqi ntws tau ua tiav rau ntawm ntau cov khoom sib tov hauv qab tib yam li qhov kev sim sine curve hauv Case 1, nrog tsuas yog tus nqi ntws hloov pauv. Cov ntaub ntawv qhia tau hais tias nyob rau hauv qhov ntau ntawm cov nqi ntws los ntawm 0.25 txog 1 ml / min, qhov pib txo qis hauv nthwv dej sine tseem nyob ruaj khov rau tag nrho peb lub ntim sib tov. Rau ob lub ntim me me sib tov, muaj qhov nce me ntsis hauv kev cog lus sinusoidal raws li tus nqi ntws txo qis, uas xav tau vim yog lub sijhawm nyob ntawm cov kuab tshuaj hauv lub sib tov, uas tso cai rau kev sib xyaw diffusion ntau ntxiv. Kev rho tawm ntawm nthwv dej sine yuav nce ntxiv raws li qhov ntws txo qis ntxiv. Txawm li cas los xij, rau qhov ntim sib tov loj tshaj plaws nrog qhov siab tshaj plaws sine nthwv dej attenuation, qhov sine nthwv dej base attenuation tseem tsis hloov pauv (nyob rau hauv qhov ntau ntawm kev tsis paub meej), nrog rau cov nqi ntawm 95% txog 98%. Mov. 10. Kev txo qis yooj yim ntawm nthwv dej sine piv rau tus nqi ntws hauv rooj plaub 1. Qhov kev sim tau ua tiav raws li cov xwm txheej zoo ib yam li qhov kev sim sine nrog tus nqi ntws sib txawv, txhaj 80% ntawm 80/20 sib tov ntawm acetonitrile thiab dej thiab 20% ntawm 20 mM ammonium acetate.
Cov khoom sib tov PerfectPeakTM inline static tshiab uas tau tsim muaj patented nrog peb lub ntim sab hauv: 30 µl, 60 µl thiab 90 µl npog qhov ntim thiab kev sib tov ua tau zoo uas xav tau rau feem ntau HPLC kev tshuaj xyuas uas xav tau kev sib tov zoo dua thiab cov plag tsev tsis sib xyaw. Lub tshuab sib tov static tshiab ua tiav qhov no los ntawm kev siv cov thev naus laus zis luam ntawv 3D tshiab los tsim cov qauv 3D tshwj xeeb uas muab kev sib tov hydrodynamic static zoo dua nrog qhov feem pua ​​​​​​tsawg tshaj plaws hauv suab nrov hauv ib chav ntawm cov khoom sib tov sab hauv. Siv 1/3 ntawm lub ntim sab hauv ntawm lub tshuab sib tov ib txwm txo cov suab nrov los ntawm 98%. Cov khoom sib tov no muaj cov channel ntws peb-seem sib txuas nrog cov cheeb tsam sib txawv thiab cov kab sib txawv thaum cov kua hla cov teeb meem geometric nyuaj sab hauv. Tsev neeg tshiab ntawm cov khoom sib tov static muab kev ua tau zoo dua li cov khoom sib tw, tab sis nrog lub ntim sab hauv tsawg dua, ua rau muaj qhov sib piv ntawm lub teeb liab thiab suab nrov qis dua, nrog rau cov duab zoo dua, kev ua haujlwm zoo dua thiab kev daws teeb meem rau kev nkag siab zoo dua.
Hauv tsab ntawv xov xwm no Chromatography - RP-HPLC uas zoo rau ib puag ncig - Siv cov chromatography core-shell los hloov acetonitrile nrog isopropanol hauv kev tshuaj xyuas thiab kev ntxuav - Cov roj chromatograph tshiab rau ...
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