Ib qho kev hloov pauv tshiab inline static tov khoom tau tsim tshwj xeeb tsim los ua kom tau raws li cov kev cai nruj ntawm kev ua haujlwm siab ua kua chromatography (HPLC) thiab kev ua haujlwm siab ua kua chromatography (HPLC thiab UHPLC).Kev sib xyaw tsis zoo ntawm ob lossis ntau lub xov tooj ntawm tes tuaj yeem ua rau muaj qhov sib piv ntawm cov teeb liab-rau-suab nrov dua, uas txo qhov rhiab heev.Homogeneous static mixing ntawm ob los yog ntau tshaj cov kua nrog qhov tsawg kawg nkaus ntim thiab lub cev qhov ntev ntawm ib tug zoo li qub tov khoom sawv cev rau lub siab tshaj plaws tus qauv ntawm ib tug zoo tagnrho static tov khoom.Tus tshiab static mixer ua tiav qhov no los ntawm kev siv 3D tshuab luam ntawv tshiab los tsim cov qauv 3D tshwj xeeb uas muab kev txhim kho hydrodynamic static sib xyaw nrog qhov feem pua ​​​​siab tshaj plaws txo qis hauv lub hauv paus sine yoj ib chav tsev ntim ntawm qhov sib tov.Siv 1/3 ntawm cov ntim sab hauv ntawm cov khoom sib xyaw ua ke txo cov sine nthwv dej los ntawm 98%.Lub tov khoom muaj kev sib cuam tshuam 3D ntws raws nrog qhov sib txawv ntawm cov ntu ntu thiab txoj hauv kev ntev raws li cov kua traverses complex 3D geometries.Kev sib xyaw ua ke ntawm ntau txoj hauv kev tortuous ntws, ua ke nrog kev kub ntxhov hauv zos thiab eddies, ua rau sib xyaw ntawm micro, meso thiab macro nplai.Qhov sib tov tshwj xeeb no yog tsim los siv cov khoom siv hluav taws xob sib xyaw ua ke (CFD) simulations.Cov ntaub ntawv xeem tau nthuav tawm qhia tias kev sib xyaw ua ke tau ua tiav nrog qhov tsawg kawg nkaus ntim.
Rau ntau tshaj 30 xyoo, kua chromatography tau siv nyob rau hauv ntau lub lag luam, xws li tshuaj, tshuaj tua kab, kev tiv thaiv ib puag ncig, forensics, thiab tshuaj ntsuam xyuas.Lub peev xwm los ntsuas rau qhov ib lab lossis tsawg dua yog qhov tseem ceeb rau kev txhim kho thev naus laus zis hauv txhua qhov kev lag luam.Kev sib xyaw ua haujlwm tsis zoo ua rau qhov tsis zoo ntawm cov teeb liab-rau-lub suab sib piv, uas yog kev ntxhov siab rau lub zej zog chromatography nyob rau hauv cov nqe lus ntawm kev kuaj pom tsis pub dhau thiab rhiab heev.Thaum sib tov ob HPLC cov kuab tshuaj, qee zaum yuav tsum yuam kev sib xyaw los ntawm lwm txoj hauv kev kom homogenize ob cov kuab tshuaj vim tias qee cov kuab tshuaj tsis sib tov zoo.Yog tias cov kuab tshuaj tsis sib xyaw kom huv si, qhov degradation ntawm HPLC chromatogram yuav tshwm sim, tshwm sim nws tus kheej raws li lub suab nrov dhau lawm thiab / lossis cov duab tsis zoo.Nrog kev sib tov tsis zoo, lub suab nrov yuav tshwm sim raws li sine yoj (nce thiab poob) ntawm lub teeb liab ntes lub sijhawm.Nyob rau tib lub sijhawm, kev sib xyaw tsis zoo tuaj yeem ua rau kev nthuav dav thiab asymmetric peaks, txo qis kev ntsuas kev ua tau zoo, cov duab ncov, thiab kev daws teeb meem siab.Kev lag luam tau lees paub tias hauv-kab thiab tee static mixers yog ib txoj hauv kev txhim kho cov kev txwv no thiab tso cai rau cov neeg siv kom ua tiav cov kev txwv qis dua (rhiab heev).Qhov zoo tshaj plaws zoo li qub tov khoom sib xyaw ua ke cov txiaj ntsig ntawm kev sib xyaw ua haujlwm siab, tsis muaj qhov ntim tuag thiab tsis tshua muaj siab poob nrog qhov tsawg kawg nkaus ntim thiab siab tshaj qhov system throughput.Tsis tas li ntawd, raws li kev soj ntsuam ua ntau nyuaj, cov kws tshuaj ntsuam xyuas yuav tsum niaj hnub siv ntau dua polar thiab nyuaj-rau-sib tov kuab tshuaj.Qhov no txhais tau hais tias kev sib xyaw ua ke zoo dua yog qhov yuav tsum tau ua rau kev sim yav tom ntej, ntxiv rau qhov xav tau kev sib xyaw ua ke zoo tshaj plaws thiab kev ua tau zoo.
Mott tsis ntev los no tau tsim ntau yam tshiab ntawm patented PerfectPeakTM inline static mixers nrog peb ntim sab hauv: 30 µl, 60 µl thiab 90 µl.Qhov ntau thiab tsawg no suav nrog ntau qhov ntim thiab cov yam ntxwv sib xyaw uas xav tau rau feem ntau ntawm HPLC cov kev xeem uas yuav tsum tau ua kom zoo sib xyaw thiab tsis muaj kev sib cais.Tag nrho peb cov qauv yog 0.5 "nyob rau hauv txoj kab uas hla thiab xa cov kev lag luam-kev ua tau zoo nyob rau hauv ib tug compact tsim.Lawv yog ua los ntawm 316L stainless hlau, passivated rau inertness, tab sis titanium thiab lwm yam corrosion resistant thiab chemically inert hlau alloys kuj muaj.Cov mixers no muaj qhov siab tshaj plaws ua haujlwm siab txog li 20,000 psi.Ntawm daim duab.1a yog ib daim duab ntawm 60 µl Mott static tov khoom tsim los muab kev sib xyaw ua haujlwm siab tshaj plaws thaum siv lub ntim me me dua li cov qauv sib xyaw ntawm hom no.Qhov tshiab static tov khoom tsim siv tshiab additive manufacturing technology los tsim ib tug tshwj xeeb 3D qauv uas siv tsawg nyob rau hauv ntws tshaj ib tug tov khoom tam sim no siv nyob rau hauv lub chromatography kev lag luam kom ua tau zoo li qub sib tov.Xws li cov khoom sib xyaw ua ke muaj kev sib txuas ntawm peb-dimensional flow channels nrog cov chaw sib txawv ntawm qhov sib txawv thiab qhov ntev ntawm txoj kev sib txawv raws li cov kua hla cov teeb meem geometric sab hauv.Ntawm daim duab.Daim duab 1b qhia txog daim duab schematic ntawm lub tshuab tov khoom tshiab, uas siv cov txheej txheem kev lag luam 10-32 threaded HPLC compression fittings rau inlet thiab qhov hluav taws xob, thiab tau ntxoov xiav ciam teb ntawm patented internal mixer chaw nres nkoj.Qhov sib txawv ntawm qhov sib txawv ntawm txoj hauv kev ntws sab hauv thiab cov kev hloov pauv hauv cov kev taw qhia ntws hauv lub ntim ntim sab hauv tsim cov cheeb tsam ntawm cov turbulent thiab laminar ntws, ua rau sib xyaw ntawm micro, meso thiab macro nplai.Kev tsim cov khoom sib xyaw tshwj xeeb no siv cov khoom siv hluav taws xob sib xyaw ua ke (CFD) simulations los txheeb xyuas cov qauv ntws thiab kho cov qauv tsim ua ntej prototyping rau hauv-tsev analytical xeem thiab cov neeg siv khoom soj ntsuam.Additive manufacturing yog cov txheej txheem luam ntawv 3D geometric Cheebtsam ncaj qha los ntawm CAD kos duab yam tsis tas yuav tsum tau siv cov tsoos machining (milling tshuab, lathes, thiab lwm yam).Cov khoom siv zoo li qub no yog tsim los tsim los siv cov txheej txheem no, qhov chaw sib tov lub cev yog tsim los ntawm CAD kos duab thiab cov khoom yog fabricated (luam tawm) txheej los ntawm txheej siv additive manufacturing.Ntawm no, ib txheej ntawm cov hmoov hlau txog 20 microns tuab yog tso, thiab lub computer tswj laser xaiv melts thiab fuses cov hmoov rau hauv ib daim ntawv.Siv lwm txheej rau saum txheej no thiab siv laser sintering.Rov ua qhov txheej txheem no kom txog rau thaum qhov ua tiav tiav.Tom qab ntawd cov hmoov raug muab tshem tawm ntawm qhov tsis-laser sib koom ua ke, tawm hauv 3D luam tawm ib feem uas phim cov duab CAD qub.Cov khoom kawg yog me ntsis zoo ib yam li cov txheej txheem microfluidic, nrog qhov sib txawv tseem ceeb yog tias cov khoom siv microfluidic feem ntau yog ob-seem (paj), thaum siv cov khoom siv ntxiv, cov qauv ntws nyuaj tuaj yeem tsim hauv peb-dimensional geometry.Cov faucets tam sim no muaj nyob rau hauv 3D luam tawm qhov chaw hauv 316L stainless hlau thiab titanium.Feem ntau cov hlau alloys, polymers thiab qee cov ceramics tuaj yeem siv los ua cov khoom siv los ntawm cov qauv no thiab yuav raug txiav txim siab rau yav tom ntej tsim / khoom.
Rice.1. Diam duab (a) thiab daim duab (b) ntawm 90 μl Mott static tov khoom uas qhia ib tug ntoo khaub lig-section ntawm lub mixer fluid flow txoj kev ntxoov ntxoo nyob rau hauv xiav.
Khiav cov kua dej sib xyaw ua ke (CFD) simulations ntawm cov khoom sib xyaw zoo li qub thaum lub sij hawm tsim qauv los pab tsim cov qauv tsim tau zoo thiab txo cov sij hawm siv nyiaj thiab kev sim ua yuam kev.CFD simulation ntawm cov khoom siv zoo li qub thiab cov kav dej txheem (tsis muaj kev sib xyaw simulation) siv COMSOL Multiphysics software pob.Kev ua qauv siv lub siab-tsav laminar fluid mechanics kom nkag siab cov dej nrawm thiab siab nyob rau hauv ib feem.Cov dej dynamics no, ua ke nrog cov tshuaj thauj mus los ntawm mobile theem tebchaw, pab kom nkag siab txog kev sib xyaw ntawm ob hom dej sib txawv.Tus qauv yog kawm raws li kev ua haujlwm ntawm lub sijhawm, sib npaug li 10 vib nas this, kom yooj yim ntawm kev suav thaum nrhiav kev sib piv.Cov ntaub ntawv theoretical tau txais nyob rau hauv ib lub sij hawm-correlated txoj kev kawm siv lub point probe projection cuab tam, qhov twg ib tug taw tes nyob rau hauv nruab nrab ntawm lub tawm tau xaiv rau sau cov ntaub ntawv.Cov qauv CFD thiab cov kev sim sim siv ob qhov sib txawv ntawm cov kuab tshuaj los ntawm cov qauv piv txwv thiab cov twj tso kua mis, uas ua rau muaj qhov hloov pauv rau txhua qhov hnyav hauv kab piv txwv.Cov kuab tshuaj no ces muab sib xyaw rau hauv ib qho khoom siv zoo li qub.Cov duab 2 thiab 3 qhia txog kev khiav dej num los ntawm cov yeeb nkab txheem (tsis muaj qhov sib tov) thiab los ntawm Mott static tov khoom, feem.Lub simulation tau khiav ntawm lub raj ncaj nraim 5 cm ntev thiab 0.25 mm ID los qhia lub tswv yim ntawm alternating plugs ntawm dej thiab ntshiab acetonitrile rau hauv lub raj thaum tsis muaj ib tug zoo li qub tov khoom, raws li qhia nyob rau hauv daim duab 2. Lub simulation siv lub pes tsawg qhov ntev ntawm lub raj thiab tov khoom thiab ib tug txaus tus nqi ntawm 0.3 ml / min.
Rice.2. Kev simulation ntawm CFD ntws nyob rau hauv 5 cm raj nrog ib txoj kab uas hla ntawm 0.25 mm los sawv cev rau dab tsi tshwm sim nyob rau hauv ib tug HPLC raj, piv txwv li nyob rau hauv tsis muaj ib tug tov khoom.Tag nrho cov liab sawv cev rau qhov loj ntawm cov dej.Xiav sawv cev rau qhov tsis muaj dej, piv txwv li acetonitrile ntshiab.Diffusion cheeb tsam tuaj yeem pom ntawm qhov sib txuas ntawm ob lub kua sib txawv.
Rice.3. Static tov khoom nrog ntim ntawm 30 ml, ua qauv hauv COMSOL CFD software pob.Cov lus dab neeg sawv cev rau qhov loj feem ntawm cov dej hauv qhov sib tov.Cov dej ntshiab yog qhia hauv liab thiab ntshiab acetonitrile hauv xiav.Kev hloov pauv hauv qhov loj feem ntawm cov dej simulated yog sawv cev los ntawm kev hloov ntawm cov xim ntawm kev sib xyaw ntawm ob cov kua dej.
Ntawm daim duab.4 qhia txog kev tshawb fawb txog kev sib raug zoo ntawm kev sib xyaw ua kom zoo thiab sib xyaw ntim.Raws li qhov sib tov ntim nce, qhov sib tov efficiency yuav nce.Rau cov kws sau ntawv kev paub, lwm yam kev ua haujlwm ntawm lub cev ua haujlwm hauv cov khoom sib tov tsis tuaj yeem suav nrog hauv CFD qauv, uas ua rau muaj kev sib xyaw ua haujlwm ntau dua hauv kev sim kev sim.Qhov kev sim sib xyaw ua haujlwm tau ntsuas raws li qhov feem pua ​​​​txo qis hauv lub hauv paus sinusoid.Tsis tas li ntawd, nce rov qab siab feem ntau ua rau muaj kev sib xyaw ntau dua, uas tsis suav nrog hauv kev simulation.
HPLC cov xwm txheej hauv qab no thiab kev sim teeb tsa tau siv los ntsuas cov sine nthwv dej los sib piv cov txheeb ze ntawm cov khoom sib txawv zoo li qub.Daim duab hauv daim duab 5 qhia txog HPLC/UHPLC system layout.Qhov sib tov zoo li qub tau sim los ntawm kev tso lub tov khoom ncaj qha tom qab lub twj tso kua mis thiab ua ntej lub tshuab txhaj tshuaj thiab cov kab sib cais.Feem ntau cov keeb kwm sinusoidal ntsuas yog ua bypassing lub injector thiab capillary kem ntawm lub static tov khoom thiab lub UV ntes.Thaum ntsuas lub teeb liab-rau-suab nrov piv thiab/los yog tshuaj xyuas lub ncov zoo, qhov system configuration yog qhia nyob rau hauv daim duab 5.
Daim duab 4. Lub ntsiab lus ntawm kev sib xyaw ua haujlwm piv rau qhov sib xyaw ua ke rau ntau yam ntawm cov khoom siv zoo li qub.Lub theoretical impurity ua raws tib qhov sib xws li cov ntaub ntawv sim impurity lees paub qhov siv tau ntawm CFD simulations.
Lub HPLC system siv rau qhov kev xeem no yog Agilent 1100 Series HPLC nrog lub UV ntes tswj los ntawm PC khiav Chemstation software.Table 1 qhia tau hais tias ib txwm tuning tej yam kev mob rau kev ntsuas tov khoom efficiency los ntawm kev soj ntsuam cov sinusoids hauv ob qhov kev tshawb fawb.Kev sim sim tau ua tiav ntawm ob qho piv txwv sib txawv ntawm cov kuab tshuaj.Ob cov kuab tshuaj sib xyaw hauv rooj plaub 1 yog hnyav A (20 mM ammonium acetate hauv dej deionized) thiab hnyav B (80% acetonitrile (ACN) / 20% deionized dej).Hauv Case 2, hnyav A yog ib qho kev daws ntawm 0.05% acetone (label) hauv dej deionized.Cov kuab tshuaj B yog sib xyaw ntawm 80/20% methanol thiab dej.Nyob rau hauv rooj plaub 1, lub twj tso kua mis tau teem rau tus nqi ntws ntawm 0.25 ml / min rau 1.0 ml / min, thiab nyob rau hauv rooj plaub 2, lub twj tso kua mis tau teem rau tus nqi ntws tsis tu ncua ntawm 1 ml / min.Hauv ob qho tib si, qhov sib piv ntawm qhov sib xyaw ntawm cov kuab tshuaj A thiab B yog 20% ​​A / 80% B. Qhov ntsuas tau teeb tsa rau 220 nm nyob rau hauv rooj plaub 1, thiab qhov siab tshaj plaws nqus ntawm acetone nyob rau hauv rooj plaub 2 tau teem rau lub wavelength ntawm 265 nm.
Table 1. HPLC Configurations for Cases 1 and 2 Case 1 Case 2 Pump Speed ​​0.25 ml/min to 1.0 ml/min 1.0 ml/min Solvent A 20 mM ammonium acetate in deionized water 0.05% Acetone in deionized water Solvent B 80% de 80% Aceton 80% dej 20% deionized dej Solvent ratio 20% A / 80% B 20% A / 80% B Detector 220 nm 265 nm
Rice.6. Cov phiaj xwm sib xyaw sine nthwv dej ntsuas ua ntej thiab tom qab siv cov lim dej qis kom tshem tawm cov hauv paus drift Cheebtsam ntawm lub teeb liab.
Daim duab 6 yog ib qho piv txwv ntawm cov suab nrov sib xyaw hauv Case 1, pom tias yog ib qho kev rov ua dua sinusoidal qauv superimposed ntawm lub hauv paus drift.Baseline drift yog qeeb nce lossis txo qis hauv cov teeb liab tom qab.Yog hais tias lub kaw lus tsis raug tso cai kom sib npaug ntev txaus, feem ntau nws yuav poob, tab sis yuav drift erratically txawm tias thaum lub kaw lus ruaj khov.Lub hauv paus drift no yuav nce ntxiv thaum lub kaw lus ua haujlwm nyob rau hauv qhov siab gradient lossis siab rov qab siab.Thaum lub hauv paus pib tam sim no, nws tuaj yeem nyuaj los sib piv cov txiaj ntsig los ntawm cov qauv mus rau cov qauv, uas tuaj yeem kov yeej los ntawm kev siv cov lim dej qis rau cov ntaub ntawv nyoos los lim tawm cov kev hloov pauv tsawg zaus, yog li muab cov phiaj xwm oscillation nrog lub hauv paus tiaj tus.Ntawm daim duab.Daim duab 6 kuj qhia tau hais tias ib zaj dab neeg ntawm lub tov khoom lub suab nrov tom qab siv lub lim dej qis.
Tom qab ua tiav CFD simulations thiab kev sim thawj zaug, peb qhov sib cais zoo li qub tau tsim kho tom qab siv cov khoom siv sab hauv uas tau piav qhia saum toj no nrog peb qhov ntim sab hauv: 30 µl, 60 µl thiab 90 µl.Qhov no suav nrog ntau qhov ntim thiab kev sib xyaw ua haujlwm uas yuav tsum tau ua rau cov ntawv txheeb xyuas HPLC tsawg uas yuav tsum tau ua kom zoo dua sib xyaw thiab tsis muaj kev sib cais los tsim cov amplitude qis.Ntawm daim duab.7 qhia txog kev ntsuas sine yoj yooj yim uas tau txais los ntawm kev ntsuas qhov ntsuas ntawm Piv txwv 1 (acetonitrile thiab ammonium acetate li tracers) nrog peb ntim ntawm cov khoom sib xyaw zoo li qub thiab tsis muaj cov khoom sib xyaw.Kev sim ntsuas cov xwm txheej rau cov txiaj ntsig tau pom hauv daim duab 7 tau tuav tas li thoob plaws hauv 4 qhov kev xeem raws li cov txheej txheem teev tseg hauv Table 1 ntawm qhov hnyav ntws ntawm 0.5 ml / min.Siv tus nqi offset rau cov datasets kom lawv tuaj yeem tso tawm ib sab ntawm ib sab yam tsis muaj teeb liab sib tshooj.Offset tsis cuam tshuam qhov amplitude ntawm lub teeb liab siv los txiav txim qhov kev ua tau zoo ntawm cov khoom sib tov.Qhov nruab nrab sinusoidal amplitude yam tsis muaj qhov sib tov yog 0.221 mAi, thaum lub amplitudes ntawm static Mott mixers ntawm 30 µl, 60 µl, thiab 90 µl poob rau 0.077, 0.017, thiab 0.004 mAi, raws li.
Daim duab 7. HPLC UV Detector Signal Offset vs. Time for Case 1 (acetonitrile with ammonium acetate indicator) qhia cov kuab tshuaj uas tsis muaj tov khoom, 30 µl, 60 µl thiab 90 µl Mott mixers uas qhia txog kev sib xyaw ua ke (qis teeb liab amplitude) raws li qhov ntim ntawm cov khoom sib tov zoo li qub.(cov ntaub ntawv tiag tiag offsets: 0.13 (tsis tov khoom), 0.32, 0.4, 0.45mA rau cov zaub zoo dua).
Cov ntaub ntawv qhia hauv daim duab.8 yog tib yam li hauv daim duab 7, tab sis lub sijhawm no lawv suav nrog cov txiaj ntsig ntawm peb feem ntau siv HPLC static mixers nrog cov ntim sab hauv ntawm 50 µl, 150 µl thiab 250 µl.Rice.Daim duab 8. HPLC UV Detector Signal Offset piv rau Lub Sijhawm Plot rau Case 1 (Acetonitrile thiab Ammonium Acetate raws li cov ntsuas) uas qhia txog kev sib xyaw ntawm cov kuab tshuaj tsis zoo li qub, cov tshiab series ntawm Mott static mixers, thiab peb cov khoom sib xyaw ua ke (cov ntaub ntawv tiag tiag offset yog 0.1 (tsis muaj tov khoom), 0.40, 8, 0.0, 0.0, 8, 0.0, mA feem rau cov txiaj ntsig zoo dua).Qhov feem pua ​​​​txo qis ntawm lub hauv paus sine yoj yog xam los ntawm qhov piv ntawm qhov amplitude ntawm sine yoj mus rau lub amplitude yam tsis muaj lub tov khoom.Qhov ntsuas sine yoj attenuation feem pua ​​​​rau Case 1 thiab 2 tau teev nyob rau hauv Table 2, nrog rau cov ntim sab hauv ntawm cov khoom siv zoo li qub thiab xya tus qauv sib xyaw uas feem ntau siv hauv kev lag luam.Cov ntaub ntawv nyob rau hauv daim duab 8 thiab 9, nrog rau cov lus qhia nyob rau hauv Table 2, qhia tau hais tias lub Mott Static Mixer muaj peev xwm muab tau mus txog 98.1% sine yoj attenuation, deb tshaj qhov kev ua tau zoo ntawm ib tug pa HPLC tov khoom nyob rau hauv cov kev sim no.Daim duab 9. HPLC UV detector teeb liab offset piv rau lub sij hawm daim phiaj rau rooj plaub 2 (methanol thiab acetone li tracers) uas qhia tsis zoo li qub tov khoom (ua ke), ib tug tshiab series ntawm Mott static mixers thiab ob pa mixers (cov ntaub ntawv tseeb offsets yog 0, 11 (tsis muaj mixer.), 0.22, 0.5, 0.3, 0.3, 0.22, 0.5, 0.3, zoo dua.Xya feem ntau siv mixers hauv kev lag luam kuj tau soj ntsuam.Cov no suav nrog cov khoom sib xyaw nrog peb qhov sib txawv sab hauv los ntawm tuam txhab A (tsim Mixer A1, A2 thiab A3) thiab tuam txhab B (tsim Mixer B1, B2 thiab B3).Tuam txhab C tsuas yog ntsuas ib qho loj.
Table 2. Static Mixer Stirring Characteristics and Internal Volume Static Mixer Case 1 Sinusoidal Recovery: Acetonitrile Test (Efficiency) Case 2 Sinusoidal Recovery: Methanol Water Test (Efficiency) Internal Volume (µl) No Tov Khoom – - 0 Mott 30 65% 602.3% Mott 30 65% 6019.2% tt 90 98.1% 97.5% 90 Tov A1 66.4% 73.7% 50 Tov A2 89.8% 91.6% 150 Mixer A3 92.2% 94.5% 250 Mixer B1 44.8% 45.7% 9.2 Mixer B1 44.8% 45.7% 9. 97.2% 97.4% 250
Kev soj ntsuam ntawm cov txiaj ntsig hauv daim duab 8 thiab Table 2 qhia tau hais tias 30 µl Mott static tov khoom muaj qhov sib xyaw ua haujlwm zoo ib yam li A1 tov khoom, piv txwv li 50 µl, txawm li cas los xij, 30 µl Mott muaj 30% tsawg dua ntim sab hauv.Thaum muab piv rau 60 µl Mott tov khoom nrog 150 µl nrog ntim A2 tov khoom, muaj kev txhim kho me ntsis hauv kev sib xyaw ua haujlwm ntawm 92% piv rau 89%, tab sis qhov tseem ceeb tshaj, qhov siab dua ntawm kev sib xyaw ua tiav ntawm 1/3 ntawm cov khoom ntim.zoo sib xws mixer A2.Kev ua tau zoo ntawm 90 µl Mott tov khoom ua raws li qhov sib xws li A3 tov khoom nrog ntim sab hauv ntawm 250 µl.Kev txhim kho hauv kev sib xyaw ua haujlwm ntawm 98% thiab 92% kuj tau pom nrog kev txo qis 3 zaug hauv ntim sab hauv.Cov txiaj ntsig zoo sib xws thiab kev sib piv tau txais rau cov khoom sib xyaw B thiab C. Raws li qhov tshwm sim, cov txheej txheem tshiab ntawm cov khoom siv zoo li qub Mott PerfectPeakTM muab kev sib xyaw ua haujlwm ntau dua li cov neeg sib tw sib piv, tab sis nrog lub ntim tsawg dua, muab cov suab nrov zoo dua qub thiab cov teeb liab-rau- suab nrov piv, zoo dua rhiab heev Analyte, ncov zoo thiab ncov daws teeb meem.Cov qauv zoo sib xws hauv kev sib xyaw ua haujlwm tau pom hauv Case 1 thiab Case 2 kev tshawb fawb.Rau Case 2, kev ntsuam xyuas tau ua los ntawm kev siv (methanol thiab acetone ua cov ntsuas) los sib piv kev sib xyaw ua haujlwm ntawm 60 ml Mott, sib piv cov khoom sib piv A1 (sab hauv ntim 50 µl) thiab cov khoom sib piv B1 (sab hauv ntim 35 µl)., kev ua tau zoo tsis zoo yam tsis muaj kev sib xyaw ua ke, tab sis nws tau siv rau kev txheeb xyuas hauv paus.Lub 60 ml Mott tov khoom ua pov thawj tias yog qhov sib tov zoo tshaj plaws hauv pawg xeem, muab 90% nce hauv kev sib xyaw ua haujlwm.Qhov sib piv Mixer A1 pom 75% kev txhim kho hauv kev sib xyaw ua haujlwm ua raws li 45% kev txhim kho hauv cov khoom sib piv B1.Ib qho yooj yim sine yoj kev ntsuas txo qis nrog tus nqi ntws tau ua tiav ntawm cov khoom sib xyaw ua ke raws li cov xwm txheej zoo ib yam li kev sim sine nkhaus hauv Case 1, tsuas yog tus nqi ntws hloov.Cov ntaub ntawv qhia tau hais tias nyob rau hauv qhov ntau ntawm tus nqi ntws los ntawm 0.25 mus rau 1 ml / min, qhov pib txo qis hauv sine yoj tseem nyob tas li rau tag nrho peb cov khoom ntim.Rau ob lub ntim me me sib tov, muaj qhov nce me ntsis hauv sinusoidal contraction raws li tus nqi ntws txo, uas yuav tsum tau vim lub sij hawm nyob rau hauv cov kuab tshuaj nyob rau hauv lub tov khoom muaj zog, cia kom muaj zog diffusion sib tov.Qhov kev rho tawm ntawm sine yoj yuav tsum nce ntxiv raws li qhov ntws qis ntxiv.Txawm li cas los xij, rau qhov sib tov loj tshaj plaws nrog qhov siab tshaj plaws sine yoj puag attenuation, sine yoj puag attenuation tseem zoo li tsis hloov pauv (nyob rau hauv qhov ntau ntawm kev sim tsis paub meej), nrog rau cov nqi ntawm 95% mus rau 98%.Rice.10. Basic attenuation of a sine wave vs flow rate in case 1. Qhov kev ntsuam xyuas tau ua nyob rau hauv cov xwm txheej zoo ib yam li sine test nrog qhov sib txawv ntawm qhov ntws, txhaj 80% ntawm 80/20 sib tov ntawm acetonitrile thiab dej thiab 20% ntawm 20 mM ammonium acetate.
Cov kev tsim tshiab tshiab ntawm patented PerfectPeakTM inline static mixers nrog peb qhov ntim sab hauv: 30 µl, 60 µl thiab 90 µl npog lub ntim thiab kev sib xyaw ua haujlwm ntau yam uas xav tau rau feem ntau HPLC kev tshuaj xyuas yuav tsum tau txhim kho kev sib xyaw thiab cov plag tsev tsis sib haum.Tus tshiab static mixer ua tiav qhov no los ntawm kev siv 3D tshuab luam ntawv tshiab los tsim cov qauv 3D tshwj xeeb uas muab kev txhim kho hydrodynamic zoo li qub sib xyaw nrog qhov feem pua ​​​​ntawm cov suab nrov tshaj plaws hauv ib chav tsev ntim ntawm cov khoom sib xyaw.Siv 1/3 ntawm cov ntim sab hauv ntawm cov khoom sib xyaw ua ke txo cov suab nrov los ntawm 98%.Xws li cov khoom sib xyaw ua ke muaj kev sib txuas ntawm peb-dimensional flow channels nrog rau qhov sib txawv ntawm qhov sib txawv thiab qhov ntev ntawm txoj kev sib txawv raws li cov kua hla cov kab nrib pleb nyuaj hauv.Cov tsev neeg tshiab ntawm cov khoom siv zoo li qub muab kev ua tau zoo dua li cov khoom sib tw sib tw, tab sis nrog cov ntim sab hauv, ua rau cov teeb liab zoo dua-rau-suab nrov piv thiab txo qis kom muaj nuj nqis, nrog rau kev txhim kho ncov zoo, kev ua haujlwm thiab kev daws teeb meem rau siab dua.
Hauv qhov teeb meem no Chromatography - Ib puag ncig tus phooj ywg RP-HPLC - Siv cov tub ntxhais-plhaub chromatography los hloov acetonitrile nrog isopropanol hauv kev tshuaj xyuas thiab kev ua kom huv - Cov roj chromatograph tshiab rau…
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