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Kuumbwa uye hunhu hwe chlorhexidine hydrochloride nanoemulsion semushonga unorwisa mabhakitiriya unopa simba rekudzivirira hutachiona: zvidzidzo zve in vitro uye ex vivo
作者 Abdelmonem R., Younis MK, Hassan DH, El-Sayed Ahmed MAEG, Hassanien E., El-Batuti K., Elfaham A.
Rehab Abdelmonem, 1 Mona K. Younis, 1 Doaa H. Hassan, 1 Mohamed Abd El-Gawad El-Sayed Ahmed, 2 Ehab Hassanein, 3 Kariem El-Batuti, 3 Alaa Elfaham 31 Sainzi neTekinoroji, Faculty of Pharmacy and Industrial Pharmacy, Misr University, 6 Gumiguru Guta, Egypt; 2 Dhipatimendi reMicrobiology and Immunology, Faculty of Pharmacy, Misr University of Science and Technology, Gumiguru 6, Egypt; 3 Dhipatimendi reEndodontics, Ain Shams University, Cairo, Egypt Nhanganyaya uye Chinangwa: Chlorine hexidine hydrochloride [Chx.HCl] ine basa rakasiyana-siyana rekurwisa mabhakitiriya, inoshanda kwenguva refu uye ine chepfu shoma, saka inokurudzirwa senzira yekudiridza midzi yemugero. Chinangwa chechidzidzo ichi chaive chekushandisa nanoemulsion itsva yeChx.HCl kuwedzera simba rinopinda, kuchenesa uye kushanda kwekurwisa mabhakitiriya kweChx.HCl uye kuishandisa senzira yekudiridza midzi yemugero. Nzira: Chx.HCl nanoemulsions dzakagadzirwa pachishandiswa mafuta maviri akasiyana: oleic acid neLabrafil M1944CS, ma surfactants maviri, Tween 20 neTween 80, uye co-surfactant, propylene glycol. Ronga dhayagiramu ye pseudo-ternary phase kuratidza system yakanaka. Ma nanoemulsion akagadzirwa akaongororwa kuti aone huwandu hwemushonga, nguva ye emulsification, kupararira, saizi yemadonhwe, kuburitswa kwemushonga mu vitro, kugadzikana kwethermodynamic, basa re antibacterial mu vitro, uye zvidzidzo zve in vitro zvemafomura akasarudzwa. Kupinda, kuchenesa uye kurwisa mabhakitiriya kweChx.HCl 0.75% ne1.6% nanoemulsion kwakaenzaniswa nehukuru hwe particles hwakajairwa se root canal irrigant. Mhedzisiro. Fomura yakasarudzwa yaive F6 ne 2% Labrafil, 12% Tween 80 ne 6% propylene glycol. Hukuru hwe particles hudiki (12.18 nm), nguva pfupi ye emulsification (1.67 seconds) uye kunyungudika nekukurumidza mushure memaminitsi maviri. Yakawanikwa kuti ine hurongwa hwakagadzikana hwe thermodynamic/physical. Kana ichienzaniswa nehukuru hwe Chx.HCl, huwandu hwakanyanya hwe Chx.HCl 1.6% nanoemulsion hwakaratidza kupinda zviri nani nekuda kwehukuru hudiki hwehunhu. Kana ichienzaniswa nehukuru hwehunhu hwehunhu (2609.56 µm2), 1.6% Chx.HCl nanoemulsion ine avhareji diki yenzvimbo yenzvimbo yakasara (2001.47 µm2). Mhedziso: Nanoemulsion composition Chx.HCl ine kugona kuchenesa zviri nani uye antibacterial action. Ine simba guru rekuuraya mabhakitiriya kurwisa Enterococcus faecalis, uye mwero wekudzivirira kwemasero ebhakitiriya wakakwira kana kuti waparadzwa zvachose. Mashoko makuru: chlorhexidine hydrochloride, nanoemulsion, root canal irrigant, kupinda, kuchenesa, antibacterial irrigant.
Nanoemulsions, rudzi rwema emulsions ane madonhwe ehukuru hwe50–500 nm, akanyanya kutariswa mumakore achangopfuura nekuda kwehunhu hwawo hwakasiyana. Hunhu hwakanaka hwekuchenesa, hahukanganisike nekuomarara kwemvura, kazhinji hune chepfu shoma uye kusavapo kwekubatana kwemagetsi. 2 Nanotechnology ine saizi diki-diki yezvikamu, chiyero chikuru chenzvimbo kusvika kuhukuru uye hunhu hwakasiyana hwemuviri nemakemikari zvichienzaniswa nezvigadzirwa zvakafanana, uye zvakare inovhura maonero matsva mukurapa nekudzivirira hutachiona hwemazino. 3 Chlorhexidine hydrochloride (Chx.HCl) inonyungudika zvishoma mumvura, inonyungudika zvishoma mualcohol uye zvishoma nezvishoma inotsvedza muchiedza. 4.5 SH. HCl ine basa rekurwisa mabhakitiriya rakafara, inoshanda kwenguva refu uye ine chepfu shoma. Nekuda kwehunhu uhwu, inokurudzirwawo senzira yekudiridza root canal. Mabhenefiti makuru eChx.HCl ichepfu shoma, hapana hwema uye hapana kuravira kusingafadzi. 6-9 Mhando dzakasiyana dze lasers dzakashandiswa kuvandudza root canal disinfection. Kuuraya mabhakitiriya kwemalaser kunoenderana nehurefu hwemafungu uye simba, pamwe nekupiswa kwemvura, izvo zvinokonzera shanduko mumadziro emasero ebhakitiriya, izvo zvinotungamira kuchinjo mu osmotic gradient kusvika pakufa kwemasero. Kudyidzana pakati pemalaser nemadiridza emidzi kunovhura horizons itsva mu pulp disinfecting. 10 Simba re ultrasonic rinogadzira ma frequency akakwira asi amplitude yakaderera. Mafaira aya akagadzirwa kuti atenderere pa ultrasound frequency ye25–30 kHz, iyo iri pamusoro pemuganhu wekunzwisisa kwekunzwa kwevanhu (>20 kHz). Mafaira aya akagadzirwa kuti atenderere pa ultrasound frequency ye25–30 kHz, iyo iri pamusoro pemuganhu wekunzwisisa kwekunzwa kwevanhu (>20 kHz). 25–30 кГц, которые находятся за пределами слухового восприкажение пределами слухового воспория восприя осправи. Mafaira aya akagadzirwa kuti adedere pa ultrasound frequency ye25-30 kHz, iyo iri kure nekunzwa kwevanhu (> 20 kHz).這些文件被设计成在25–30 kHz 的超声波频率下振荡，這超出了人类听觉感知的极限(>20 kHz).這些文件被设计成在 25–30 kHz Файлы рассчитаны на колебания на ультразвуковых частотах 25–30 кГц, что выходит за пределы слухового восприятия человека (>). Mafaira aya akagadzirirwa kudedera pa ultrasound frequency ye25-30 kHz, iyo iri kunze kwemiganhu yekunzwa kwevanhu (>20 kHz).Dzinoshanda mu transverse oscillation, dzichiisa maitiro emhando yemapfundo uye antinodes pakureba kwawo. Izwi rekuti "passive ultrasonic irrigation" (PUI) inzira yekudiridza umo hapana zvishandiso kana madziro anosangana nemafaira e endodontic kana zvishandiso. Munguva yePUI, simba re ultrasound rinotamiswa kubva kufaira rinodedera kuenda kumvura yekudiridza iri mu root canal. Izvi zvinogona kukonzera kuyerera kwenzwi uye cavitation ye flushing agent. 11 Zvichibva padata riri pamusoro apa, zvinoonekwa zvakakodzera kushandisa nanotechnology kuongorora kushanda kwakavandudzwa kweChx.HCl kupinda nekuchenesa.
Chlorhexidine hydrochloride Chx.HCl yakapihwa nemutsa neArab Drug Company for Pharmaceuticals (Cairo, Egypt). Labrafil M 1944 CS (oleoylpolyoxy-6-glyceride) yakapihwa nerupo neGattefosse (Saint Priest, France). Pakati pe20 (polyoxyethylene (20) sorbitan monolaurate), Tween 80 (polyoxyethylene (80) sorbitan monooleate), oleic acid, propylene glycol kubva kuGomhorya Company (Cairo, Egypt)). Kubviswa kwemazino asina kuodza mwoyo ekurapa periodontal kana orthodontic, Department of Maxillofacial Sciences, Faculty of Dentistry, Ain Shams University, Cairo, Egypt. Pure culture of Enterococcus faecalis (strain ATCC 29212) inorimwa mumuto webrain heart extract (BHI) (RC CLEANER, IIchung Dental Ltd., Seoul, Korea).
Kunyungudika kweChx.HCl muzvinhu zvakasiyana-siyana (oleic acid, Labrafil M 1944CS, Tween 20, Tween 80, propylene glycol, nemvura) kwakadzidzwa. Chx.HCl yakawanda (50 mg) inoiswa muchubhu yecentrifuge uye 5.0 g yechikamu chepakati inowedzerwa. Musanganiswa wacho wakazununguswa mumuchina wevortex kwemaminitsi gumi nemashanu wobva wachengetwa patembiricha yemukamuri. Mushure memaawa makumi maviri nemana, pellet yemishonga isinganyungudike iri muchubhu yakatenderedzwa pa3000 rpm kwemaminitsi mashanu kuti iwane supernatant yakajeka. Unganidza mhinduro yakakwana yemuenzaniso woisanganisa ne n-butanol. Sampuli dzakasanganiswa dzakaseferwa kuburikidza nepepa rekusefa reWhatman 102 uye dzakazosanganiswa zvakakodzera ne n-butanol kuti dzione huwandu hwemushonga mumushonga wakazara. Sampuli dzakaongororwa ne UV spectrophotometer pa260 nm ne n-butanol senzira yekudzora. 12.13
Dhayagiramu yechikamu chepseudo-triple yakavakwa kuti ione chiyero chaicho chechikamu chimwe nechimwe chinodiwa muchimiro kuti uwane maparamita akakodzera e nanoemulsion yakakodzera. 14 Mushonga uyu wakagadzirwa uchishandisa mafuta (kureva oleic acid neLabrafil M1944CS), ma surfactants (kureva Tween 20 neTween 80) uye imwe surfactant, kureva propylene glycol. Kutanga, musanganiswa wakaparadzaniswa wema surfactants (asina cosurfactants) nemafuta wakagadzirwa muhuwandu hwakasiyana (kubva pa1:9 kusvika 9:1). Kana musanganiswa wacho waiswa mvura (uchiwedzera mvura ichidonha), nyatso tarisa musanganiswa wacho kubva pakuchena kusvika pakusviba senzvimbo yekupedzisira. Aya ma points ekupedzisira anobva anyorwa padhayagiramu yechikamu chepseudo-triple. Maitiro ese akadzokororwa kune musanganiswa we surfactant ne secondary surfactant (Smix) yakagadzirwa mu 2:1 ne 3:1 ratios uye yakasanganiswa nemafuta akasarudzwa15,16 imwe.
Masisitimu eNanoemulsion ane Chx.HCl akagadzirwa achishandisa Labrafil M 1944 CS sechikamu chemafuta uye Tween 80 kana 20 surfactant uye propylene glycol sechikamu chekuwedzera surfactant uye pakupedzisira mvura, Tafura 1. Mushonga uyu wakanyungudutswa muLabrafil M 1944 CS uye mvura yakabatana yesurfactant neyechipiri surfactant yakawedzerwa zvishoma nezvishoma nekusanganiswa zvishoma nezvishoma. Huwandu hwesurfactant neco-surfactant yakawedzerwa, pamwe nechikamu chechikamu chemafuta chinogona kuwedzerwa, chinoonekwa uchishandisa dhayagiramu ye pseudo-ternary phase. Jenareta ye ultrasonic (Ultrasonic LC 60 H, Elma, Germany) yakashandiswa kuwana huwandu hwehukuru hwaidiwa hwekuparadzira granules. Wobva waenzanisa. 17
Kuongororwa kwekupararira kwakaitwa pachishandiswa muchina wekunyungudutsa (Dr. Schleuniger Pharmaton, Model Diss 6000, Thun, Switzerland) umo 1 ml yemushonga wega wega yakawedzerwa ku500 ml yemvura pa37±0.5°C. Kunyungudutsa zvinyoro nyoro kunovimbiswa nemidziyo yekunyungudutsa yesimbi isingasvibi inotenderera pa50 rpm. Musanganiswa wakabuda wakaonekwa nemaziso uye wakaiswa muzvikamu zvakajeka, zvinopenya zvine ruvara rwebhuruu, zvakaita semukaka kana kuti zvine madzerere. Sarudza fomura yakajeka yekutsvagisa kwakawedzerwa. 18.19
Kubviswa kweChx.HCl kubva muzvinhu zvakagadzirwa nenanoemulsion zvichibva padhayagiramu yepseudo-triple phase kunotungamira mukugadzirwa kwen-butanol uchishandisa tekinoroji yeultrasonic. Mushure mekudirwa kwakakodzera, zvinyorwa zvakaongororwa nespectrophotometrically pawavelength ye260 nm kuti zvionekwe kuti zviri muChx.HCl. makumi maviri.
Kuti kuyedze nguva yekuzvisanganisa, 1 ml yemusanganiswa wega wega yakawedzerwa mubhodhoro rakazadzwa ne250 ml yemvura yakasanganiswa uye yakachengetwa pa37 ± 1°C nekukurunga nguva dzose pa50 rpm. Nguva yekuzvisanganisa inotorwa senguva iyo preconcentrate inoumba musanganiswa wakafanana mushure mekusanganiswa. Makumi maviri nerimwe
Kuti muongorore saizi yemadonhwe, sanganisai 50 mg yemushonga wakagadzirwa zvakanaka kusvika pa1000 ml nemvura mubhodhoro mosanganisa zvinyoro nyoro nemaoko. Kugoverwa kwehukuru hwemadonhwe kwakaongororwa uchishandisa mudziyo weMalvern Zetasizer 2000 (Malvern Instruments Ltd., Malvern, UK) pasi pemamiriro ekuona kumashure kwe173º, tembiricha ye25ºC, uye refractive index ye1.330.22.
Zvidzidzo zvekunyungudika kwemushonga we in vitro zvakaitwa pachishandiswa USP Type II apparatus (paddle) (Dr. Schleuniger Pharmaton, Diss Model 6000) pa50 rpm. Mvura yakanyungudika (500 ml) yakachengetwa patembiricha ye37±0.5°C yakashandiswa senzira yekunyungudika, uye 5 ml yemusanganiswa wakagadzirwa yakawedzerwa dropwise kune nzira yekunyungudika. Zvadaro, panguva dzakasiyana-siyana, 5 ml yenzira yekunyungudika yakatorwa uye huwandu hwemushonga wakaburitswa hwakaongororwa ne spectrophotometrically pa 254 nm. Kuedza kwakaitwa katatu. 23
Zvadaro, ma parameter e kinetic ekuburitswa kweChx.HCl mu vitro kubva ku nanoemulsions akagadzirwa zvichibva pahwaro hwayo akayerwa. Zero, first and second order kinetics uye Higuchi diffusion models zvakaedzwa kusarudza kinetic sequence yakakodzera kuburitswa kweChx.HCl.
2 ml yemushonga wega wega yakachengetwa patembiricha yekunze kwemaawa makumi mana nemasere isati yaonekwa kupatsanurwa kwechikamu. 1 ml yemushonga wega wega weChx.HCl nanoemulsion yakasanganiswa kusvika pa10 ml uye 100 ml nemvura yakasanganiswa pa25° C. uye yakachengetwa kwemaawa makumi maviri nemana. Zvadaro kupatsanurwa kwechikamu kwakaonekwa. 21
Zvadaro, 2 ml yemusanganiswa wega wega yakaendeswa yoga mumabhodhoro akajeka ane chivharo chescrew ndokuchengetwa mufiriji pa2°C kwemaawa makumi maviri nemana. Zvadaro yakabviswa ndokuchengetwa pa25°C ne40°C. Kutenderera kwekutonhodza kamwe chete kwakaitwa. Mienzaniso yacho yakazoongororwa kuti ionekwe kana paine kupatsanurwa kwechikamu uye mvura yaibva. Makumi maviri nerimwe
Sampuro ye5 ml yeChx.HCl nanoemulsion formula yega yega yakaendeswa muchubhu yegirazi ndokuiswa mulabhoritari centrifuge (Shanghai Surgical Instrument Factory Microcentrifuge Model 800, Shanghai, People's Republic of China) uye yakatenderedzwa pa4000 rpm kwemaminitsi mashanu. Sampuro dzakazoongororwa kuti dzionekwe kuti dzinoparadzanisa sei uye kuti dzinobvisa mvura. Makumi maviri nerimwe.
Kuedza kwese kwakatenderwa neInstitutional Ethics Committee yeAin Shams University, Egypt. Mazino makumi mashanu emunhu asina kutyisa ane mudzi mumwe chete ane apex yakagadzirwa akasarudzwa. Mazino akabviswa akashandiswa mushure mekunge murwere awana mvumo yakanyorwa yakasainwa. Mazino anosanganisira maxillary ne mandibular incisors uye mandibular premolars. Nzvimbo dzekunze dzemidzi dzakarapwa ne curette uye mazino ese akacheneswa pamusoro mu 0.5% NaOCl kwemaawa makumi maviri nemana ndokuzochengetwa mu sterilize saline kusvika yashandiswa. Korona yakabviswa ne safe side diamond disc uye kureba kwezino kwakagadziriswa kusvika 16 mm kubva pamusoro kusvika pa coronal margin. 24,25 Zvichienderana nemushonga wekusuka, mazino akakamurwa mumapoka anotevera:
(A) Sampuli dzeboka (n=24) dzakagezwa neChx.HCl nanoemulsion. Sampuli dzeboka (I) (n=12) dzakagezwa ne5 ml yeChx.HCl nanoemulsion 0.75%. Sampuli dzeboka (II) (n=12) dzakagezwa ne5 ml ye1.6% yeChx.HCl nanoemulsion. (B) Sampuli dzeboka (n=24) dzichagezwa ne5 ml ye2% yeChx.HCl normal particle size. Boka rekudzora: (n=2) dzakagezwa ne5 ml yesaline pasina kuisimbisa.
Mazino emunhu makumi mana nemana asina kutyisa ane midzi imwe chete akasarudzwa. Mazino aya anosanganisira mazino epamusoro neapamusoro uye mandibular premolars. Pamusoro pemidzi pakapihwa mushonga wekudzivirira mazino uye mazino ese akacheneswa pamusoro ne0.5% NaOCl kwemaawa makumi maviri nemana uye akazochengetwa mu sterilize saline kusvika ashandiswa. Makorona akabviswa nesafety diamond disc uye kureba kwezino kwakagadziriswa kusvika 16 mm kubva pamusoro kusvika pamucheto we coronal. 24,25,29
Kugadzirira faira guru reapical saizi 50 uchishandisa nzira dzakajairwa. Shandisa sterilize saline semushonga wekudiridza panguva yekuvhiyiwa. Pakupedzisira, mugero wemidzi wakasukwa ne 2 ml ye 17% EDTA kweminiti imwe chete kubvisa smear layer. Pamusoro pemudzi wese, kusanganisira apical foramen yemuenzaniso wega wega, wakafukidzwa ne coat mbiri dze nail polish (cyanoacrylate glue) kudzivirira kubuda kwemvura. Mazino acho anoiswa akatwasuka mu block ye tartar kuti zvive nyore kubata uye kuziva. 29-33 sampuro dzakazoiswa otomatiki pa 121ºC uye 15 psi kwemaminetsi makumi maviri. Mushure mekuvhiyiwa, sampuro dzese dzakatakurwa uye dzakagadziriswa pasi pemamiriro esterilize uchishandisa zvishandiso zvesterilize. Mugero wemidzi wakasvibiswa ne culture yakachena ye Enterococcus faecalis (strain ATCC 29212) yakadyarwa mumuto we brain heart extract (BHI) kwemaawa makumi maviri nemana pa 37°C. Uchishandisa micropipette isina utachiona, isa mushonga weE. faecalis inoculum wakajeka mumigero yemidzi yemazino ese yakagadzirwa. Mabhuroko acho akaiswa mumidziyo isina utachiona uye oiswa pa37°C kwemaawa makumi maviri nemana. 31, 34, 35
(A) Samples dzeboka (n=24) dzakagezwa neChx.HCl nanoemulsion. Samples dzeboka diki (I) (n=12) dzakagezwa ne5 ml yeChx.HCl nanoemulsion 0.75% concentration. Subgroup (II) (n = 12) dzakagezwa ne5 ml yeChx.HCl nanoemulsion 1.6% concentration.
Boka rekudzora: kudzora kwakarurama, (n=4) mugero wemidzi wakasvibiswa wakasukwa ne 5 ml yemunyu ndokuchengetwa senzira yekudzora kwakarurama. Kudzora Kwakaipa: (n=4) Mienzaniso haina kubaiwa ne suspension, kureva kuti mugero wemidzi hauna kusvibiswa ne E. faecalis, uye yakachengetwa isina kuchena senzira yekudzora kwakaipa kusimbisa kuvharwa kwemasero uye kuvimbika kwenzira yacho. Shandisa 5 ml yekuyedza wash solution mumuenzaniso wega wega. Muenzaniso wega wega wakazosukwa kekupedzisira ne 1 ml yemunyu wakasvibiswa.
Pepa remhando ye35 sterile rinoshandiswa kuunganidza sampuro kubva mumidzi yemidzi. Pepa repamusoro rakaiswa muchubhu kusvika pakureba kwayo, rikasiyiwa kwemasekonzi gumi, rozoendeswa kumaplate eagar kuti rione huwandu hwemayuniti ekugadzira koloni (CFU) paplate imwe neimwe. Maplate akachengetwa pa37ºC kwemaawa makumi maviri nemana uye ozoongororwa nemaziso kuti mabhakitiriya akura here. Pepa rakajeka rinoratidza kuvharwa kwebhakitiriya kwakakwana. Maplate akasviba anoonekwa seanoratidza kukura kwakanaka. Avhareji yehuwandu hweCFU munzvimbo yekukura kwebhakitiriya padhishi imwe neimwe yakaongororwa uye huwandu hweCFU hwakaverengerwa. Maplate akapona anoyerwa zvakanyanya nekuverenga kwakanaka pamaplate emvura. Pamusoro pezvo, kapu yekudurura yakashandiswa kuverenga CFU dzakaderera, uye kuderedzwa kusvika ku106 kwakashandiswa kuverenga CFU dzakakwira. 36.37
Gadzirira machubhu ane 15 ml ye agar medium yakanyungudutswa isati yaongororwa mu autoclave pazuva rimwe chete nerekuyedza. Enterococcus faecalis i facultative Gram-positive anaerobic coccus inogona kurarama kana pH yakakwira, acidity uye tembiricha yakakwira. 39 Mabhakitiriya samples (Enterococcus faecalis ATCC 29212) akagadzirwa nekusanganisa masero kubva kumakoloni ne sterile saline. Mabhakitiriya samples akazosanganiswa ne saline kuti ienderane ne McFarland 0.5, yakaenzana ne 108 CFU/mL. Sample volume yakawedzerwa yaive 10 µl. 39 Turbidity standard (McFarland 0.5)40 yakagadzirwa nekudurura 0.6 ml ye 1% (10 g/l) barium chloride dihydrate solution mu 100 ml graduated cylinder uye kuzadza kusvika 100 ml ne 1% (10 g/l) sulfuric acid. Matanho ekudziya akaiswa mumachubhu akafanana neaya emuto uye akachengetwa patembiricha yemukamuri kwemwedzi mitanhatu murima uye akavharirwa kudzivirira kupwa kwemvura. Vhura chivharo chedhishi rePetri risina chinhu wodururira muenzaniso pakati pedhishi. Kana agar yaoma zvachose, shandura ndiro woiisa pa37°C kwemaawa makumi maviri nemana.
Data rese rakaunganidzwa, rakarongwa uye rakaongororwa nenhamba. Kuongororwa kwenhamba kwakaitwa uchishandisa IBM® SPSS® Statistical Version 17 yeWindows (SPSS Inc., IBM Corporation, Armonk, NY, USA).
Kunyungudika kweChx.HCl muzvikamu zvakasiyana-siyana zvemafuta, mhinduro dze surfactant, mhinduro dze co-surfactant, uye mvura zvakadzidzwa. Chx.Hcl ine kunyungudika kwakanyanya muLabrafil M uye kunyungudika kwakaderera mu oleic acid. Kunyungudika kwakanyanya kwemishonga muchikamu chemafuta kwakakosha kune nanoemulsions nekuti nanoemulsions dzinokwanisa kuchengetedza mushonga muchimiro chakanyungudika, zvichireva kuti kunyungudika kwakanyanya kwemishonga mumafuta kunoguma nemafuta mashoma muchimiro uye nekudaro mushonga mushoma. kurodha Huwandu hwakati hwe surfactant ne co-surfactant hunodiwa kuti madonhwe emafuta anyungudike.
Dhayagiramu yechikamu chepseudo-triple yakagadzirwa kuti itsanangure nzvimbo dze nanoemulsion uye kugadzirisa huwandu hwemafuta akasarudzwa, ma surfactants, uye mamwe ma surfactants (Labrafil M, Tween 80, Tween 20, uye propylene glycol, zvichiteerana). Chx.Hcl inoratidza kunyungudika kwakaderera mu oleic acid, zvichikonzera kudonha kwemakore kana oleic acid yaiswa titration nedonhwe rekutanga remvura. Nokudaro, oleic acid system haina kubviswa muchidzidzo ichi. Mamwe maformula akagadzirwa achishandisa musanganiswa we1:9 wemafuta ne surfactant. huwandu hwe pH uye ionic strength, saka ma surfactants aya akasarudzwa.
Mafomula ese akagadzirwa aive akajeka kunze kweSystem F2, iyo yairatidzika kunge ine makore saka haina kutorwa muzvidzidzo zvekuongorora.
Nzira yakanaka yekubvisa nanoemulsion inofanira kukwanisa kupararira zvachose uye nekukurumidza kana yaderedzwa nekukasira. Nzira dzekubvisa nanoemulsion dzeChx.HCl dzakaratidza nguva pfupi yekubvisa emulsification, kubva pamasekondi 1.67 kusvika 12.33. Pakati pe80 pane nguva pfupi yekubvisa emulsification. Izvi zvinogona kutsanangurwa nekukwanisa kwakakwirira kwekunyungudutsa kweTween 80. Nguva yekuzvinyungudutsa inowedzera nekuwedzera kwehuwandu hwe surfactant, izvo zvinogona kunge zvichikonzerwa nekuwedzera kwe viscosity yesystem pasi pekushanda kwe surfactant.
Hukuru hwemadonhwe emulsion hunosarudza mwero uye huwandu hwekuburitswa kwemushonga. Hukuru hwemadonhwe emulsion hudiki hunoita kuti nguva ye emulsification ive pfupi uye nzvimbo yakawanda yekunyudzwa kwemushonga ive pamusoro. Avhareji yehukuru hwemadonhwe emhando dzakasarudzwa dzeChx.HCl nanoemulsion dzaive 711±0.44, 587±15.3, 10.97±0.11, 16.43±4.55, uye 12.18±2.48, uye PDI yaive 0 .76, 0.19, 0.61, 0.47 uye 0.76 yeF1, F2., F3 uye 0.16 zvichiteerana F4, F5 uye F6. Mafomula ane Tween 80 sesurfactant akaratidza maspherulites madiki. Izvi zvinogona kunge zvichikonzerwa nesimba rayo re emulsifying rakawanda. PDI value yakaderera inoratidza kugoverwa kwehukuru hwesystem. Mafomu aya ane chitarisiko chakachena nekuti madonhwe awo madiki pane marefu echiedza chinooneka (390-750 nm) apo chiedza chinopararira zvishoma.
Pamufananidzo wechipiri inoratidza huwandu hweChx.HCl inoburitswa kubva mufomura yakagadzirwa. Kuburitswa kwemushonga wese kubva mumafomura akagadzirwa eChx.HCl nanoemulsion kwaive kubva pamaminitsi maviri kusvika manomwe. Zvakaonekwa kuti mwero wepamusoro wekuburitswa kwemushonga wakawanikwa muchiitiko cheChx.HCl F6 nanoemulsion (2 min), izvo zvinogona kunge zvichikonzerwa nekuvapo kweTween 80, iyo yakaratidza mwero wepamusoro we emulsification, uye nanoemulsion inobuda. inopa nzvimbo yakakura yekuburitswa kwemushonga, zvichibvumira mwero wekuwedzera wekuburitswa kwemushonga. Panguva imwe chete, hunhu hwekunyungudika kwepropylene glycol hunobvumira huwandu hwakawanda hwehydrophilic surfactants kunyungudutswa mumafuta. 40
Kuburitswa kweChx.HCl mu vitro kwakawanikwa kuchitevera kurongeka kwakasiyana kwekinetic, uye hapana kurongeka kwakajeka kwekinetic kunogona kuratidza kuburitswa kwemishonga kubva kumafomura e nanoemulsion akagadzirwa zvakasiyana. Kuburitswa kwekinetic kwemishonga yeF4 ndiko kinetics yekutanga, zvinoreva kuti inoburitswa zvichienderana nehuwandu hwemushonga huri mukati mayo. 42 Kuburitswa kwekinetic kwemamwe mishonga kwaienderana neHiguasha diffusion model, iyo yakaratidza kuti huwandu hwemushonga unoburitswa hwaienderana ne square root yemushonga wese uye kunyungudika kwemushonga mu nanoemulsion. 42
Mafomula akasarudzwa akaiswa mukugadzikana kwakasiyana-siyana kwethermodynamic kuburikidza nekuyedzwa kwekushushikana uchishandisa ma "heat-cool cycles", "centrifugation" uye "freeze-thaw cycles". Zvakaonekwa kuti ma "formulations" F3 na F4 akaratidza kunaya kwemushonga mushure mekunyunguduka, nepo F1 yakaratidza kukora (gelling). F5 na F6 vakapasa "continuous centrifugation cycle", "heating-cooling test" uye "freeze-thaw test". Ma "nanoemulsions" masisitimu akagadzikana muthermodynamics akagadzirwa pane mamwe mafuta, "surfactant" nemvura pasina "phase separation", "emulsification" kana "cracking". Kugadzikana kwethermal ndiko kunosiyanisa ma "nanoemulsions" kubva kuma "emulsions", ayo akasimba mu "kinetically" uye anozopedzisira aparadzana kuita "phases". 19 F3 yakaratidza saizi yakakura ye "particle size" (587 nm) kupfuura mamwe ma "formulations", izvo zvinogona kutsanangura kupatsanurwa kwechikamu uye "drug precipitation" mu "thermodynamic stability tests". F4 ine Tween 80 uye isina "co-surfactant" yakaratidza kunaya kwemushonga, izvi zvinogona kuratidza kudiwa kwekushandisa propylene glycol naTween 80 kuti ivandudze kugadzikana kwe "nanoemulsion formulations". F1 ine Tween 20 isina surfactant yakawedzerwa yakaratidza kukora (gelling), izvo zvinoreva kuwedzera kwekusimba kwegel nekuda kwekuungana kwemadonhwe.
Mhedzisiro yekugadzikana inoratidza kukosha kwekuvapo kweimwe propylene glycol surfactant yekuwedzera kupararira kwezvikamu uye kudzivirira kunaya kwemvura mumishonga. 43 F6 ndiyo yaive fomura yakanakisisa nekuda kwehukuru hwezvikamu zvidiki (12.18 nm), nguva pfupi ye emulsification (1.67 seconds) uye kukurumidza kunyungudika mushure memaminitsi maviri. Yakawanikwa iri sisitimu yakagadzikana mu thermodynamically/physical uye saka yakasarudzwa kuti iongororwe zvakanyanya.
Kukundikana mushure mekurapwa kwemidzi yetsvina kuri kuramba kuchiwanda, zvichireva kuti varwere vari panjodzi yakakura yekuva nezvirwere zvakaoma. 44,45 Biofilm inofanira kubviswa panguva yekuuraya utachiona uye kuzadza midzi yetsvina. 46,47 Nekuda kwekuoma kwemidzi yetsvina, zvinova zvakaoma kubvisa midzi yetsvina yetsvina yetsvina yetsvina uchishandisa zvishandiso chete uye kudiridza. 48 Kubudirira kwemvura inosuka midzi yetsvina kunoenderana nekupinda kwemudiridziri muDT uye nguva yekusangana nemabhakitiriya. 49 Saka, nzira itsva dzekuchenesa midzi yetsvina dzakaedzwa uye dzakaedzwa. Kushambidza kwakajairika hakubvisi zvachose E. faecalis nekuda kwekupinda kushoma kweDT.50.
Avhareji yesimba rekuchenesa remushonga wekuchenesa nanoemulsion raive 2001.47 µm2, uye avhareji yehukuru hwezvimedu zvemushonga wekuchenesa raive 2609.56 µm. Musiyano wepakati pemushonga wekuchenesa nanoemulsion newashing yakajairika yezvimedu waive 608.09 µm2. Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa ane (P-value 0.00052). Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa ane (P-value 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частиц наблюдалась статистически зна0,0ма разница (значение P 0,00052). Paive nemusiyano wakakosha zvikuru (P<0.001) (P kukosha 0.00052) pakati pezvinodiridza zve nanoemulsion nezvinodiridza zve normal particle.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P<0.001） 0.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P<0.001） 0. Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически очень значимая разниц0000 (P10) 0,00052). Paiva nemusiyano wakakosha zvikuru (P<0.0001) pakati pe nanoemulsion rinse neyakajairika particle size rinse (P value 0.00052).Nanoemulsion yakaratidza musiyano wakakosha zvikuru muhuwandu kana tichienzanisa nehukuru hwezvinhu zvakajairika, ichiratidza nzvimbo yakaderera yepakati yemarara, kureva kuti zvinhu zvenanoemulsion zvaive nekugona kuchenesa kwakanyanya, sezvakaratidzwa pamufananidzo 3.
Mufananidzo 3. Kuenzanisa mashandiro ekuchenesa kwezvinobatsira pakushambidza: (A) neNano CHX laser yakabatidzwa, (B) neCHX laser yakabatidzwa, (C) nePUI Nano CHX, (D) isina Nano CHX activation, (E) isina CHX activation, uye (F) ) CHX PUI activation.
Avhareji yenzvimbo yepamusoro yezvimedu zveChx.HCl 1.6% yasara yaive 2320.36 µm2, uye avhareji yenzvimbo yepamusoro yeChx.HCl 2% yaive 2949.85 µm2. Paiva nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu (P-value 0.00000). Paiva nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu (P-value 0.00000). Наблюдалась статистически высокозначимая (P<0,001) разница между более высокой концентрацией наноэмульсионных ирригацион ирригационными растворами с нормальным размером частиц (значение P 0,00000). Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa (P kukosha 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的Ｎ显着的Ｎ1 ¿0.00000.较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学显着的差异（001P0. Наблюдалась статистически очень значимая разница (P<0,001) между более высокими концентрациями ополаскивателя с наноэмульпесь нормальным размером частиц (значение P 0,00000). Paiva nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwe nanoemulsion rinse uye saizi yakajairika ye particle rinse (P kukosha 0.00000).Kunyangwe huwandu hwe nanoemulsion irrigant hwaive hwakaderera pane hwe particle size irrigant yakajairika, huwandu hwakaderera uhwu hwaive hwakanyanya kushanda mukubvisa marara uye hunoshanda zvakanyanya mukuchenesa midzi yemugero.
PUI yaive nemusiyano wakakosha zvikuru muhuwandu (p<0.001) kana ichienzaniswa nedzimwe nzira dzekushandisa. PUI yaive nemusiyano wakakosha zvikuru muhuwandu (p<0.001) kana ichienzaniswa nedzimwe nzira dzekushandisa. PUI имел статистически высокозначимую разницу (p<0,001) по сравнению с другими методами активации. PUI yaive nemusiyano wakakosha zvikuru muhuwandu (p<0.001) zvichienzaniswa nedzimwe nzira dzekushandisa.与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001).与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001). По сравнению с другими методами активации PUI имел статистически значимую разницу (p<0,001). Zvichienzaniswa nedzimwe nzira dzekushandisa, PUI yaive nemusiyano wakakosha zvikuru muhuwandu (p<0.001).Nekushanda kweISP, avhareji yenzvimbo yakasara yenzvimbo yemarara yaive 1695.31 µm2. Musiyano wepakati pakati pePUI neLaser waive 987.89929 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne (p-value 0.00000). Musiyano wepakati pakati pePUI neLaser waive 987.89929 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne (p-value 0.00000). Средняя разница между PUI uye Laser составила 987,89929, демонстрируя высокостатистически значимую (P<0,001) разницу с (p-значение 000). Musiyano wepakati pakati pePUI neLaser waive 987.89929, zvichiratidza musiyano wakakosha zvikuru (P<0.001) kubva ku (p-value 0.00000). PUI 和Laser 之间的平均差异為987.89929，显示出高度统计学显着性(P<0.001) 差异(p 值0.0000).PUI uye Laser Средняя разница между PUI uye Laser составила 987,89929, что свидетельствует о высокой статистической значимости (P<0,001) разниц00 (P<0,001) разниц00 (P<0,001) разни000 Musiyano wepakati pakati pePUI neLaser waive 987.89929, zvichiratidza kukosha kwakanyanya kwehuwandu (P<0.001) mutsauko (p-value 0.00000). Musiyano wepakati pakati pePUI nekusava neactivation waive 712.40643 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne p-value ye 0.00098). Kushandiswa kweLaser activation kana kusaita activation kwakanga kusina kusiyana zvakanyanya ne statistically (P>0.05) ne P-value ye 0.451211. Musiyano wepakati pakati pePUI nekusava neactivation waive 712.40643 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne p-value ye0.00098). P-value ye0.451211. Средняя разница между PUI uye отсутствием активации составила 712,40643, демонстрируя высокостатистически значимую (P<0,001) p<0,001 0,00098). Musiyano wepakati pakati pePUI nekusava neactivation waive 712.40643, zvichiratidza musiyano wakakosha zvikuru (P<0.001) ne p-value ye0.00098).P-kukosha 0.451211. PUI 和未激活之间的平均差异為712.40643，显示高度统计学显着性差异(P<0.001)，p 0006430).PUI Средняя разница между PUI и нактивацией составила 712,40643, что свидетельствует о высокой статистической значимости разниз001,
Avhareji yesimba rekuchenesa remushonga wekuchenesa nanoemulsion raive 2001.47 µm2, uye avhareji yehukuru hwezvimedu zvemushonga wekuchenesa raive 2609.56 µm. Musiyano wepakati pemushonga wekuchenesa nanoemulsion newashing yakajairika yezvimedu waive 608.09 µm2. Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa ane (P-value 0.00052). Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa ane (P-value 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частиц была статистически высоя00зназна,мальным размером (значение P 0,00052). Paive nemusiyano wakakosha zvikuru (P<0.001) (P kukosha 0.00052) pakati pezvinodiridza zve nanoemulsion nezvinodiridza zve normal particle.纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0（001（P<0.000P<0.000). P <0.001 (P值0.00052). Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически очень значимая разниц0000 (P10) 0,00052). Paiva nemusiyano wakakosha zvikuru (P<0.0001) pakati pe nanoemulsion rinse neyakajairika particle size rinse (P value 0.00052).Zvichienzaniswa nechinhu chakajairika chehukuru hwezvikamu, nanoemulsion ine musiyano wakakosha zvikuru, ichiratidza nzvimbo yakaderera yepakati pemarara akasara, kureva kuti Nanoemulsion ine kugona kuri nani kuchenesa sezvakaratidzwa muMufananidzo 3.
Avhareji yenzvimbo yepamusoro yezvimedu zveChx.HCl 1.6% yasara yaive 2320.36 µm2, uye avhareji yenzvimbo yepamusoro yeChx.HCl 2% yaive 2949.85 µm2. Paive nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e nanoemulsion ne madiridziro ehukuru hwezvimedu zvakajairika (P-value 0.00000). Paiva nemusiyano wakakosha zvikuru (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e-nanoemulsion nemadiridziro ehukuru hwezvimedu (P-value 0.00000). P<0,001 растворами с нормальным размером частиц (значение P 0,00000). Paive nemusiyano wakakosha (P<0.001) pakati pehuwandu hwakanyanya hwemadiridziro e nanoemulsion nemadiridziro ehukuru hwezvimedu zvakajairwa (P kukosha 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）(P值0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的Ｎ显着的Ｎ显着的0显着的0显着. Наблюдалась статистически высокозначимая разница (P <0,001) между более высокими концентрациями ополаскивателя с наноэмульпиями нормальным размером частиц (значение P 0,00000). Paiva nemusiyano wakakosha zvikuru (P < 0.001) pakati pehuwandu hwakanyanya hwe nanoemulsion rinse uye saizi yakajairika ye particle rinse (P kukosha 0.00000).Kunyangwe huwandu hwe nanoemulsion irrigant hwaive hwakaderera pane hwe particle size irrigant yakajairika, huwandu hwakaderera uhwu hwaive hwakanyanya kushanda mukubvisa marara uye hunoshanda zvakanyanya mukuchenesa midzi yemugero.
PUI yaive nemusiyano wakakosha zvikuru (p<0.001) kana ichienzaniswa nedzimwe nzira dzekushandisa. PUI yaive nemusiyano wakakosha zvikuru (p<0.001) kana ichienzaniswa nedzimwe nzira dzekushandisa. PUI имел статистически высокую значимую разницу (p<0,001) по сравнению с другими методами активации. PUI yaive nemusiyano wakakosha pahuwandu (p<0.001) zvichienzaniswa nedzimwe nzira dzekushandisa.与其他激活方法相比，PUI 具有统计学上的显着差异(p<0.001). Kana tichienzanisa nedzimwe nzira dzekushandisa, PUI ine musiyano wakakosha muhuwandu (p<0.001). PUI статистически значимо отличался (p<0,001) по сравнению с другими методами активации. PUI yaive yakasiyana zvakanyanya nenhamba (p<0.001) zvichienzaniswa nedzimwe nzira dzekushandisa.Munguva yekushanda kwePUI, avhareji yenzvimbo yemarara epamusoro yaive 1695.31 μm2. Musiyano wepakati pakati pePUI neLaser waive 987.89929 uchiratidza musiyano wakakosha kwazvo (P<0.001) ne (p-value 0.00000). Musiyano wepakati pakati pePUI nekusava neactivation waive 712.40643 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne (p-value 0.00098). Kushandiswa kweLaser activation kana kusaita activation kwaive kusina musiyano wakakosha (P>0.05) ne (P-value 0.451211). Musiyano wepakati pakati pePUI neLaser waive 987.89929 uchiratidza musiyano wakakosha kwazvo (P<0.001) ne (p-value 0.00000). Musiyano wepakati pakati pePUI nekusava neactivation waive 712.40643 uchiratidza musiyano wakakosha zvikuru (P<0.001) ne (p-value 0.00098). Kushandiswa kweLaser activation kana kusaita activation kwaive kwakasiyana zvakanyanya ne (P>0.05) ne (P-value 0.451211). Средняя разница между PUI и лазером составила 987,89929, демонстрируя высокостатистически значимую (P<0,001) разницу с (p00,001) разницу с (p00,00). Musiyano wepakati pakati pePUI nelaser waive 987.89929, zvichiratidza musiyano wakakosha zvikuru (P<0.001) ne (p-value 0.00000). . - kukosha 0.00098). Kushandiswa kwekushandiswa kwelaser activation kana kusashandiswa kwe activation kwaiva nemusiyano wakakosha muhuwandu (P>0.05) ne (P-kukosha 0.451211). PUI 和激光之间的平均差异為987.89929，与(p 值0.00000) 差异具有高度统计学意义(P<0.001) Musiyano wepakati pePUI nelaser i987.89929, uye musiyano (p 值0.00000) une kukosha kukuru kwehuwandu (P<0.001). Средняя разница между PUI uye лазером составила 987,89929, что было высоко статистически значимым (P<0,001) с (значение p 0,000). Musiyano wepakati pakati pePUI nelaser waive 987.89929, izvo zvaive zvakakosha zvikuru muhuwandu (P<0.001) ne (p value 0.00000). PUI 与未激活之间的平均差异為712.40643，与(p) 差异具有高度统计学意义(P<0.001.001) -0 090. Musiyano wepakati pePUI neasina kushanda i712.40643, uye musiyano (p) une kukosha kukuru kwehuwandu (P<0.001) - kukosha 0.00098. Средняя разница между PUI и нактивацией составила 712,40643, что было высоко статистически значимым с разницей (p) (P<0,0001 —). Musiyano wepakati pakati pePUI neinactivation waive 712.40643, izvo zvaive zvakakosha zvikuru muhuwandu hwemasvomhu nemusiyano (p) (P<0.001 - kukosha 0.00098).使用激光激活或不激活没有显着统计学差异(P>0.05) 与(P 值0.451211). Pakanga pasina musiyano mukuru wehuwandu pakati pekushandiswa kwelaser nekusava kushanda (P>0.05) uye (P 值0.451211). Не было статистически значимой разницы (P>0,05) по сравнению с (значение P 0,451211) с лазерной активацией или без нее. Pakanga pasina musiyano wakakosha muhuwandu (P>0.05) zvichienzaniswa ne (P kukosha 0.451211) nekushandiswa kwelaser kana pasina.Avhareji yenzvimbo yepamusoro yezvimedu zvakasara panguva yekushandiswa kwelaser yaive 2683.21 μm2. Avhareji yenzvimbo yepamusoro yezvimedu zvakasara zvisina kuvhurwa yaive 2407.72 μm2. Kana tichienzanisa nekushandiswa kwelaser kana kusashandiswa, PUI ine avhareji yenzvimbo yepamusoro yechip iri diki, kureva kugona kuchenesa kuri nani.
Mhedzisiro yepakati ye nanoemulsion sushi pakubviswa kwemarara yaive yakakwira zvakanyanya muhuwandu kupfuura ye sushi yehukuru hwezvimedu. Chx.HCl 1.6%, PUI 1938.77 µm2, 2510.96 µm2 ne laser. Pasina activation, avhareji yemutengo i2511.34 µm2. Pakashandiswa 2% Chx.HCl uye yakabatidzwa ne laser, mhedzisiro yacho yaive yakaipisisa uye huwandu hwemarara hwaive hwakanyanya. Mhedzisiro imwechete yakawanikwa apo 0.75% Chx.HCl isina kubatidzwa. Zviri pachena kuti mhedzisiro yakanaka yakawanikwa uchishandisa huwandu hwakawanda hwe sushi aid mu nanoemulsion. PUI yainyanya kushanda mu irrigant activation uye kusukwa kwemarara, sezvakaratidzwa muMufananidzo 3A-F)).
Sezvakaratidzwa muTafura 2, nanoemulsion yeChx.HCl yakashanda zviri nani pane zvidimbu zvehukuru hwakajairika maererano nehuwandu hwezvinhu zvipenyu uye yaive nehukama hwakanaka nekupinda kwemushonga uye kuchenesa zvinoenderana nematanho anotevera: saizi, huwandu hwemushonga wekutsvaira uye nzira yekushanda.
Mabhakitiriya anogona kuparadzwa zvachose nekushandisa mushonga wekubatsira pakushambidza wakawanda. Kunyangwe nePUI activation, 0.75% Chx.HCl yaive nemhedzisiro yakaipa yekurwisa mabhakitiriya. Kushanda neLaser kune mhedzisiro yakaipa pakuchenesa kwenano-emulsion. Sezvatinoona kubva pane zvese zvakabuda kare, kushandiswa kwelaser kunoderedza kushanda kweChx.HCl 0.75% nanoemulsion, uko CFU ye nanoChx.HCl 0.75% iri 195, inova kukosha kwakanyanya, zvichiratidza kuti ma reagents ari pahuwandu uhwu akafanana nekushanda nelaser. Ma diode lasers ane photothermal, saka chiedza kana kupisa zvinogona kukonzera nanoemulsion kurasikirwa nemhedzisiro yayo yekurwisa mabhakitiriya. Mhedzisiro yehuwandu hwakawanda kuparadzwa kwakazara kwebhakitiriya. Nano Chx.HCl 1.6% yakaratidza kukura kwebhakitiriya kusina kunaka kana paine laser activation, zvinoreva kuti laser haina kukanganisa kugona kwe nano Chx.HCl 1.6%. Zvinogona kugumiswa kuti nanoemulsion material ine huwandu hwakawanda ine mhedzisiro iri nani yekurwisa mabhakitiriya.
Mubasa iri, maChx.HCl nanoemulsions akagadzirwa achishandisa mafuta maviri akasiyana, masurfactants maviri uye co-surfactant, fomura yakanakisisa (F6) ine saizi diki yezvikamu, nguva pfupi ye emulsification uye mwero wepamusoro wekunyungudika) yakasarudzwa. Pamusoro pezvo, (F6) yakaedzwa kugadzikana kwe thermodynamic/physical. MuChx.HCl nanoemulsion pahuwandu hwe1.6%, Chx.HCl nanoemulsion yakaratidza kupenya kwakanakisa mumatubhu emazino zvichienzaniswa neChx.HCl yechinyakare semvura yekuchenesa, uye PUI senzira yekuchenesa yaive nekugona kuchenesa. Pamusoro pezvo, zvidzidzo zveChx.HCl nanoemulsion zvakaratidza kubviswa kwebhakitiriya zvachose. Mhedzisiro yacho yakasimbisa izvi. Chx.HCl nanoemulsion inogona kutariswa semvura yekugeza inovimbisa.
Tinotenda zvikuru vashandi verabhoritari yekutsvagisa yeMisr University of Science and Technology nerutsigiro rwavo rukuru.
Nguva yekutumira: Nyamavhuvhu-08-2022
