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Te hanganga me te whakaahuatanga o te nanoemulsion chlorhexidine hydrochloride hei whakamakuku awa pakiaka huakita whai hua: ngā rangahau in vitro me ex vivo
作者 Abdelmonem R., Younis MK, Hassan DH, El-Sayed Ahmed MAEG, Hassanien E., El-Batuti K., Elfaham A.
Rehab Abdelmonem, 1 Mona K. Younis, 1 Doaa H. Hassan, 1 Mohamed Abd El-Gawad El-Sayed Ahmed, 2 Ehab Hassanein, 3 Kariem El-Batuti, 3 Alaa Elfaham 31 Pūtaiao me te Hangarau, Te Wāhanga Rongoā me te Rongoā Ahumahi, Te Whare Wānanga o Misr, 6 Oketopa City, Ihipa; 2 Tari Microbiology me te Immunology, Te Wāhanga Rongoā, Te Whare Wānanga o Misr o te Pūtaiao me te Hangarau, 6 Oketopa, Ihipa; 3 Tari Endodontics, Te Whare Wānanga o Ain Shams, Cairo, Ihipa Whakataki me te Kaupapa: He whānui te mahi patu huakita o te chlorine hexidine hydrochloride [Chx.HCl], he roa te mahi, ā, he iti te paitini, nō reira e taunakihia ana hei whakamakuku pakiaka pea. Ko te whāinga o tēnei rangahau he whakamahi i tētahi hanganga hou o te Chx.HCl nanoemulsion hei whakanui ake i te kaha o te wero, te horoi me te mahi patu huakita o Chx.HCl, ā, hei whakamahi hei whakamakuku pakiaka. Ngā Tikanga: I whakaritea ngā nanoemulsion Chx.HCl mā te whakamahi i ngā hinu rerekē e rua: te waikawa oleic me te Labrafil M1944CS, ngā surfactants e rua, ko Tween 20 me Tween 80, me te co-surfactant, ko propylene glycol. Tuhia he hoahoa wāhanga pseudo-ternary hei tohu i te pūnaha tino pai. I aromatawaihia ngā hanganga nanoemulsion kua whakaritea mō te ihirangi rongoā, te wā emulsification, te marara, te rahi o te pata, te tukunga rongoā in vitro, te pumau thermodynamic, te mahi antibacterial in vitro, me ngā rangahau in vitro o ngā hanganga kua tohua. I whakaritea te mahi wero, te horoi me te antibacterial o te Chx.HCl 0.75% me te 1.6% nanoemulsion ki te rahi matūriki noa hei whakamakuku awa pakiaka. Ngā Hua. Ko te hanganga i whiriwhiria ko F6 me te 2% Labrafil, 12% Tween 80 me te 6% propylene glycol. He iti te rahi matūriki (12.18 nm), he poto te wā emulsification (1.67 hēkona) me te tere o te rewa i muri i te 2 meneti. Kua kitea he pūnaha pumau ā-ira/ā-tinana. Ki te whakatauritea ki te rahi matūriki Chx.HCl tikanga, ko te kukū teitei ake o te nanoemulsion Chx.HCl 1.6% i whakaatu i te urunga pai ake nā te iti o te rahi matūriki. Ki te whakatauritea ki te rauemi rahi matūriki noa (2609.56 µm2), ko te nanoemulsion Chx.HCl 1.6% te iti rawa o te horahanga mata toharite o ngā para toenga (2001.47 µm2). Whakatau: He pai ake te kaha horoi me te mahi patu huakita o te hanganga Nanoemulsion Chx.HCl. He tino whai hua te mahi patu huakita ki a Enterococcus faecalis, ā, he teitei, he tino whakangaromia rānei te tere whakaheke pūtau huakita. Ngā kupu matua: chlorhexidine hydrochloride, nanoemulsion, whakamakuku awa pakiaka, urunga, pānga horoi, whakamakuku patu huakita.
Ko ngā Nanoemulsion, he momo emulsion me te rahi o ngā pata i roto i te awhe 50–500 nm, kua nui te aro ki ngā tau tata nei nā ō rātou āhuatanga ahurei. He pai ngā āhuatanga horoi, kāore e pāngia e te pakeke o te wai, i te nuinga o te wā he iti te paitini me te kore o ngā taunekeneke hiko. 2 He iti rawa te rahi o te matūriki o te Nanotechnology, he nui te ōwehenga o te mata ki te papatipu me ngā āhuatanga ā-tinana me te matū ahurei ki te whakataurite ki ngā hua papatipu rite, ā, ka whakatuwhera hoki i ngā tirohanga hou ki te maimoatanga me te aukati i ngā mate niho. 3 He paku wairewa te Chlorhexidine hydrochloride (Chx.HCl) i roto i te wai, he paku wairewa noa i roto i te waipiro, ā, ka āta poke i te mārama. 4.5 SH. He whānui te mahi patu huakita a te HCl, he roa te mahi me te iti o te paitini. Nā ēnei āhuatanga, e taunakihia ana hoki hei whakamakuku awa pakiaka pea. Ko ngā painga matua o Chx.HCl he iti te paitini pūtau, kāore he kakara, kāore hoki he reka kino. 6-9 He maha ngā momo taiaho kua whakamahia hei whakapai ake i te patu huakita awa pakiaka. Ko te pānga patu huakita o ngā taiaho e whakawhirinaki ana ki te roanga ngaru me te kaha, tae atu ki te pānga wera, e puta ai ngā huringa i roto i te pakitara pūtau huakita, e arahi ana ki te huringa o te rerenga wai tae noa ki te matenga o te pūtau. Ko te taunekeneke i waenga i ngā taiaho me ngā whakamakuku awa pakiaka ka whakatuwhera i ngā pae hou ki te patu huakita. 10 Ka puta i te kaha o te ultrasonic ngā auau teitei engari he iti ngā kaha. Kua hangaia ngā kōnae kia wiriwiri i ngā auau ultrasonic o te 25–30 kHz, e kore nei e taea e te tirohanga a te tangata (>20 kHz). Kua hangaia ngā kōnae kia wiriwiri i ngā auau ultrasonic o te 25–30 kHz, e kore nei e taea e te tirohanga a te tangata (>20 kHz). Файлы предназначены для колебаний на ультразвуковых частотах 25–30 кГц, которые находятся за пределами слух слух человека (> 20 кГц). Kua hangaia ngā kōnae kia wiriwiri i ngā auau ultrasonic o te 25-30 kHz, e kore nei e taea e te taringa tangata te whakarongo (> 20 kHz).这些文件被设计成在25–30 kHz 的超声波频率下振荡，这超出了人类听觉感知)的(>0陈感知)的(>0陈知)的(>2陈矐。这些文件被设计成在 25–30 kHz Файлы рассчитаны на колебания на ультразвуковых частотах 25–30 кГц, что выходит за пределы слухового восприятися кГц). Kua hangaia ngā kōnae mō ngā wiri i ngā auau ultrasonic o te 25-30 kHz, arā, kei tua atu i ngā rohe o te whakarongo tangata (>20 kHz).Ka mahi rātou i roto i te oscillation whakawhiti, ka whakatakoto i ngā momo āhuatanga o ngā pona me ngā antinodes i tō rātou roa. Ko te kupu "passive ultrasonic irrigation" (PUI) he tikanga whakamakuku e kore ai he taputapu, he pakitara rānei e pa ki ngā kōnae endodontic, ki ngā taputapu rānei. I te wā o te PUI, ka whakawhitia te kaha ultrasound mai i te kōnae wiri ki te otinga whakamakuku i roto i te awa pakiaka. Ka taea e tēnei te whakaputa i te rere oro me te cavitation o te kaihoko horoi. 11 I runga i ngā raraunga o runga ake nei, e kiia ana he tika te whakamahi i te nanotechnology hei aromatawai i te mahi whakauru me te horoi pai ake o Chx.HCl.
I tukuna mai te Chlorhexidine hydrochloride Chx.HCl e te Arab Drug Company for Pharmaceuticals (Kairo, Ihipa). I tukuna mai te Labrafil M 1944 CS (oleoylpolyoxy-6-glyceride) e Gattefosse (Saint Priest, France). Tween 20 (polyoxyethylene (20) sorbitan monolaurate), Tween 80 (polyoxyethylene (80) sorbitan monooleate), waikawa oleic, propylene glycol mai i te Gomhorya Company (Kairo, Ihipa)). Te tango i ngā niho pakiaka kotahi kāore i te pirau mō te maimoatanga periodontal, orthodontic rānei, Tari Pūtaiao Maxillofacial, Faculty of Dentistry, Ain Shams University, Kairo, Ihipa. Te ahurea parakore o te Enterococcus faecalis (momo ATCC 29212) i whakatipuria i roto i te hupa tangohanga roro ngākau (BHI) (RC CLEANER, IIchung Dental Ltd., Seoul, Korea).
I tirotirohia te wairewatanga o Chx.HCl i roto i ngā momo pāpāho (waikawa oleic, Labrafil M 1944CS, Tween 20, Tween 80, propylene glycol, me te wai). Ka whakanohoia he nui rawa o Chx.HCl (50 mg) ki roto i te ngongo centrifuge, ā, ka tāpirihia he 5.0 g o te wāhanga reo. I rūrūtia te ranunga i roto i te mīhini whakaranu vortex mō te 15 meneti, kātahi ka rongoatia ki te pāmahana rūma. I muri i te 24 hāora, ka centrifuged te pire rongoā kore-wairewa i roto i te ngongo i te 3000 rpm mō te 5 meneti kia puta he wai mata mārama. Kohia he otinga tauira e ranea ana, ka whakaranua ki te n-butanol. I tātarihia ngā tauira kua whakaranua mā te pepa tātari Whatman 102, kātahi ka whakaranua kia tika ki te n-butanol hei whakatau i te kukū o te rongoā i roto i te otinga kua mākū. I tātarihia ngā tauira ki te spectrophotometer UV i te 260 nm me te n-butanol hei mana whakahaere. 12.13
I hangaia he tūtohi āhua-toru-pēnei hei whakatau i te ōwehenga tika o ia wāhanga e hiahiatia ana i roto i te whakatakotoranga hei whiwhi i ngā tawhā tino pai o te nanoemulsion tino pai. 14 I hangaia te whakatakotoranga mā te whakamahi i ngā hinu (arā, te waikawa oleic me te Labrafil M1944CS), ngā matū horoi (arā, Tween 20 me Tween 80) me tētahi atu matū horoi, arā, te propylene glycol. Tuatahi, i whakaritea ngā ranunga motuhake o ngā matū horoi (kāore he matū horoi takirua) me ngā hinu i roto i ngā ōwehenga rōrahi rerekē (mai i te 1:9 ki te 9:1). Ina titrated te ranunga ki te wai (tāpirihia he wai maturuturu), aroturukihia te ranunga mai i te mārama ki te kapua hei pito whakamutunga. Kātahi ka tohua ēnei pito whakamutunga ki runga i te tūtohi āhua-toru-pēnei. I whakahokia te tukanga katoa mō ngā ranunga o te matū horoi me te matū horoi tuarua (Smix) i whakaritea i roto i ngā ōwehenga 2:1 me te 3:1, ā, i konatunatua ki ngā hinu kua tohua15,16 kotahi.
I whakaritea ngā pūnaha nanoemulsion kei roto ko Chx.HCl mā te whakamahi i te Labrafil M 1944 CS hei wāhanga hinu, me te Tween 80, 20 rānei hei kaiwhakaora me te propylene glycol hei kaiwhakaora tāpiri, ā, i te mutunga ko te wai, Ripanga 1. I whakarewaina te rongoā ki roto i te Labrafil M 1944 CS, ā, i tāpirihia te wai whakakotahi o te kaiwhakaora me te kaiwhakaora tuarua i te tere puhoi me te whakaranu āta haere. Ko te nui o te kaiwhakaora me te kaiwhakaora-tahi i tāpirihia, me te ōrau o te wāhanga hinu ka taea te tāpiri, ka whakatauhia mā te whakamahi i te hoahoa wāhanga pseudo-ternary. I whakamahia he kaihanga ultrasonic (Ultrasonic LC 60 H, Elma, Tiamana) hei whakatutuki i te awhe rahi e hiahiatia ana mō te horapa i ngā granules. Kātahi ka taurite. 17
I whakahaerehia te whakamātautau marara mā te whakamahi i tētahi taputapu whakarewa (Dr. Schleuniger Pharmaton, Model Diss 6000, Thun, Switzerland) i tāpirihia ai te 1 ml o ia whakaritenga ki te 500 ml o te wai i te 37±0.5°C. Ka āta whakaohokia mārire mā ngā hoe whakarewa maitai kore waikura paerewa e hurihuri ana i te 50 rpm. I whakatauhia te emulsion hua mā te tirohanga, ā, i whakarōpūtia he mārama, he mārama me te tae kikorangi, he miraka, he kohu rānei. Kōwhiria he tātai mārama mō ētahi atu rangahau. 18.19
Ko te tangohanga o Chx.HCl mai i ngā hanganga nanoemulsion kua arotauhia i runga i te hoahoa wāhanga pseudo-toru ka arahi ki te hanga o te n-butanol mā te whakamahi i te hangarau ultrasonic. I muri i te waimehatanga tika, i tātarihia ngā tangohanga mā te spectrophotometrical i te roanga ngaru o te 260 nm mō te ihirangi o Chx.HCl. e rua tekau.
Hei whakamātautau i te wā whakapūmau-ā-roto, i tāpirihia te 1 ml o ia hanganga ki roto i tētahi beaker kua kī tonu i te 250 ml o te wai whakapūmau, ā, i puritia ki te 37 ± 1°C me te whakaoho tonu i te 50 rpm. Ko te wā whakapūmau-ā-roto ka tangohia hei te wā e hanga ai te preconcentrate i tētahi ranunga ōrite i muri i te waimeha. rua tekau mā tahi
Mō te tātaritanga rahi o te pata, whakaranua te 50 mg o te whakatakotoranga kua arotauhia ki te 1000 ml ki te wai i roto i te pounamu, ā, whakaranua mārire mā te ringaringa. I whakatauhia te tohatoha rahi o te pata mā te whakamahi i te taputapu Malvern Zetasizer 2000 (Malvern Instruments Ltd., Malvern, UK) i raro i ngā tikanga kitenga marara whakamuri o te 173º, te pāmahana o te 25ºC, me te taupū whakaata o te 1.330. rua tekau mā rua.
I whakahaerehia ngā rangahau whakarewatanga in vitro mā te whakamahi i tētahi taputapu USP Type II (hoe) (Dr. Schleuniger Pharmaton, Diss Model 6000) i te 50 rpm. I whakamahia te wai whakamātao (500 ml) i puritia i te pāmahana o te 37±0.5°C hei reo whakarewa, ā, i tāpirihia he 5 ml o te hanganga kua whakaritea ki te reo whakarewa. Kātahi, i ngā wā rerekē, ka tangohia he 5 ml o te reo whakarewa, ā, ka whakatauhia te nui o te rongoā i tukuna mā te spectrophotometric i te 254 nm. I whakahaerehia ngā whakamātautau i roto i ngā tāruarua e toru. rua tekau mā toru
Kātahi ka inehia ngā tawhā nekeneke o te tukunga o Chx.HCl i roto i te vitro mai i ngā nanoemulsion i whakaritea i runga i tōna pūtake. I whakamatautauria ngā nekeneke kore, te tuatahi me te tuarua o ngā tauira, me ngā tauira horapa Higuchi hei whiriwhiri i te raupapa nekeneke e tino pai ana mō te tukunga o Chx.HCl.
I rongoatia te 2 ml o ia whakatakotoranga i te pāmahana taiao mō ngā hāora 48 i mua i te kitenga i te wehenga ā-wāhanga. Kātahi ka whakaranua ngā tauira 1 ml o ia whakatakotoranga nanoemulsion Chx.HCl ki te 10 ml me te 100 ml ki te wai whakapūkara i te 25° C. ā, ka rongoatia mō ngā hāora 24. Kātahi ka tirohia te wehenga ā-wāhanga. rua tekau mā tahi
Kātahi ka whakawhitia motuhaketia ngā tauira o te 2 ml o ia hanganga ki roto i ngā pounamu mārama me te taupoki tīwiri, ā, ka rongoatia ki roto i te pouaka whakamātao i te 2°C mō ngā hāora 24. Kātahi ka tangohia, ka rongoatia ki te 25°C me te 40°C. I whakahaerehia he huringa whakamatao-whakarewa kotahi. Kātahi ka tirohia ngā tauira mō te wehenga wāhanga me te whakatōtōtanga o te rongoā. rua tekau mā tahi
I whakawhitia he tauira 5 ml o ia hanganga nanoemulsion Chx.HCl ki roto i tētahi ngongo karāhe, ā, i whakanohoia ki roto i tētahi centrifuge taiwhanga (Shanghai Surgical Instrument Factory Microcentrifuge Model 800, Shanghai, People's Republic of China) ā, i centrifugehia i te 4000 rpm mō te 5 meneti. Kātahi ka tirohia ngā tauira mō te wehenga ā-wāhanga me te whakapūmautanga o te rongoā. rua tekau mā tahi
I whakaaetia ngā whakamātautau katoa e te Komiti Matatika Whare Wānanga o Ain Shams, Ihipa. E 50 ngā niho tangata kotahi-pūtake kāore i te pirau, me te pito kua hangaia, i tīpakohia. I whakamahia ngā niho i tangohia i muri i te whiwhinga whakaaetanga tuhituhi me te hainatanga a te tūroro. Kei roto i ngā niho ko ngā niho tapahi maxillary me te mandibular me ngā premolar mandibular. I rongoatia ngā mata o waho o ngā pakiaka ki te curette, ā, i tukuna ngā niho katoa ki te sterilization mata i roto i te 0.5% NaOCl mō ngā haora 24, kātahi ka rongoatia ki roto i te tote sterile tae noa ki te whakamahinga. I tangohia te karauna ki te kōpae taimana taha haumaru, ā, i whakatauritea te roa o te niho ki te 16 mm mai i te pito ki te tawhē coronal. 24,25 E ai ki te otinga horoi, ka wehea ngā niho ki ngā rōpū e whai ake nei:
(A) I horoia ngā tauira rōpū (n=24) ki te nanoemulsion Chx.HCl. I horoia ngā tauira o te rōpū iti (I) (n = 12) ki te 5 ml nanoemulsion Chx.HCl kukū 0.75%. I horoia ngā tauira e te rōpū iti (II) (n=12) ki te 5 ml o te nanoemulsion Chx.HCl 1.6%. (B) Ka horoia he rōpū (n=24) o ngā tauira ki te 5 ml 2% Chx.HCl rahi matūriki noa. Rōpū whakahaere: (n=2) i horoia ki te 5 ml tote me te kore whakahohe.
I tīpakohia e 44 ngā niho tangata, kotahi te pūtake, kāore i te pirau, me te pito kua hangaia. Kei roto i ngā niho ko ngā niho tapahi o runga me raro, me ngā niho mua o raro. I rongoatia ngā mata o waho o ngā pūtake ki te curette, ā, i whakahoromatatia ngā niho katoa ki te 0.5% NaOCl mō ngā hāora 24, kātahi ka rongoatia ki te tote parakore tae noa ki te whakamahinga. I tangohia ngā karauna ki te kōpae taimana haumaru, ā, i whakatauritea te roa o te niho ki te 16 mm mai i te tihi ki te taha korona. 24,25,29
Te whakarite ā-ringa o te kōnae apical matua rahi 50 mā te whakamahi i ngā tikanga paerewa. Whakamahia te tote parakore hei whakamakuku i te wā pokanga. Hei whakamutunga, i horoia te awa pakiaka ki te 2 ml o te 17% EDTA mō te 1 meneti hei tango i te paparanga paru. Ko te mata pakiaka katoa, tae atu ki te pūrua apical o ia tauira, i hipokina ki ngā paparanga e rua o te peita whao (cyanoacrylate glue) hei ārai i te turuturu. Kātahi ka whakatakotoria ngā niho ki roto i tētahi poraka tartar hei ngāwari ake te whakahaere me te tautuhi. 29-33 Kātahi ka whakamahanahia ngā tauira ki te 121ºC me te 15 psi mō te 20 meneti. I muri i te whakamahana, ka haria ngā tauira katoa, ā, ka tukatukahia i raro i ngā āhuatanga parakore mā te whakamahi i ngā taputapu parakore. I pokehia ngā awa pakiaka ki te ahurea parakore o Enterococcus faecalis (momo ATCC 29212) i whakatipuria i roto i te hupa tangohanga ngākau roro (BHI) mō te 24 hāora i te 37°C. Mā te whakamahi i tētahi micropipette parakore, werohia he wairewa mārama o te E. faecalis inoculum ki roto i ngā awa pakiaka kua whakaritea o ngā niho katoa. Kātahi ka whakanohoia ngā poraka ki roto i ngā ipu parakore, ā, ka whaowhia ki te 37°C mō ngā hāora 24. 31, 34, 35
(A) I horoia ngā tauira rōpū (n=24) ki te nanoemulsion Chx.HCl. I horoia ngā tauira o te rōpū iti (I) (n=12) ki te 5 ml o te nanoemulsion Chx.HCl kukū 0.75%. I horoia ngā tauira e te rōpū iti (II) (n = 12) ki te 5 ml o te nanoemulsion Chx.HCl kukū 1.6%.
Rōpū whakahaere: mana whakahaere pai, (n=4) i horoia te awa pakiaka kua pangia ki te 5 ml tote, ā, i puritia hei mana whakahaere pai. Mana Whakahaere Kino: (n=4) Kāore i werohia ngā tauira ki te wai whakatārewatanga, arā, kāore te awa pakiaka i pangia e te E. faecalis, ā, i puritia kia ma hei mana whakahaere kino hei whakaū i te whakahoromata me te pono o te tikanga. Whakamahia he 5 ml wai horoi whakamātautau ki ia tauira. Kātahi ka horoia ia tauira ki te 1 ml tote ma.
Ka whakamahia he pito pepa parakore rahi 35 hei kohi tauira mai i ngā awa pakiaka. I whakaurua te pito pepa ki roto i te ngongo kia rite te roa, ka waiho mō te 10 hēkona, kātahi ka whakawhitia ki ngā pereti agar hei whakatau i te maha o ngā waeine hanga koroni (CFU) mō ia pereti. I whakatōngia ngā pereti ki te 37ºC mō te 24 hāora, kātahi ka arotakehia te tipu o te huakita. Ka whakaatuhia e te pereti mārama te parakoretanga katoa. E kiia ana ko ngā pereti pōuriuri he tipu pai. I whakatauhia te maha toharite o ngā CFU i roto i te rohe tipu huakita mō ia rihi, ā, i tatauhia te maha o ngā CFU. Ko ngā toenga ka inehia mā te tatau ora i runga i ngā pereti maturuturu. Hei tāpiri, i whakamahia he kapu ringihanga hei tatau i ngā CFU iti, ā, i whakamahia he waimeha ki te 106 hei tatau i ngā CFU teitei. 36.37
Whakaritea ngā ngongo kei roto te 15 ml o te agar-agar kua rewa kua whakahoromatatia i roto i te autoclave i te rā kotahi i whakahaerehia ai te whakamātautau. Ko te Enterococcus faecalis he coccus anaerobic Gram-positive facultative ka taea te ora i te pH tino teitei, te waikawa me te pāmahana teitei. 39 I whakaritea ngā tauira huakita (Enterococcus faecalis ATCC 29212) mā te whakaranu i ngā pūtau mai i ngā koroni me te tote whakahoromata. Kātahi ka whakawaiwaitia ngā tauira huakita ki te tote kia rite ki te McFarland 0.5, he rite ki te 108 CFU/mL. Ko te rōrahi tauira i tāpirihia he 10 µl. 39 I whakaritea he paerewa turbidity (McFarland 0.5)40 mā te ringihia te 0.6 ml o te 1% (10 g/l) otinga barium chloride dihydrate ki roto i te rango 100 ml me te whakakī ki te 100 ml ki te 1% (10 g/l) waikawa whanariki. I whakanohoia ngā paerewa paru ki roto i ngā ngongo kotahi i whakamahia ai ngā tauira hupa, ā, i rongoatia ki te pāmahana rūma mō te 6 marama i roto i te pōuri, ā, i hiritia kia kore ai e mimiti. Whakatuwheratia te taupoki o te rihi Petri kau, ka ringihia te tauira ki waenganui o te rihi. Mena kua totoka katoa te agar, hurihia te pereti ka whakatōkia ki te 37°C mō te 24 hāora.
I kohia ngā raraunga katoa, i tātaitia, ā, i tātarihia ngā tatauranga. I whakamahia te IBM® SPSS® Statistical Version 17 mō Windows (SPSS Inc., IBM Corporation, Armonk, NY, USA) hei tātari tatauranga.
I rangahauhia te wairewatanga o Chx.HCl i roto i ngā momo wāhanga hinu, ngā otinga surfactant, ngā otinga co-surfactant, me te wai. Ko Chx.Hcl te wairewatanga teitei rawa atu i roto i te Labrafil M, ā, ko te wairewatanga iti rawa atu i roto i te waikawa oleic. He mea nui te wairewatanga teitei ake o te rongoā i roto i te wāhanga hinu mō ngā nanoemulsions nā te mea ka taea e ngā nanoemulsions te pupuri i te rongoā i roto i te āhua wairewa, ko te tikanga ko te wairewatanga teitei ake o te rongoā i roto i te hinu ka hua ake he iti ake te hinu i roto i te hanganga, nō reira he iti ake te rongoā. te utaina Me whai nui te surfactant me te co-surfactant hei whakaemulsi i ngā pata hinu.
I hangaia he tūtohi wāhanga rūpahu-toru hei tautuhi i ngā rohe nanoemulsion me te arotau i ngā kukū o ngā hinu kua tīpakohia, ngā surfactants, me ngā surfactants tāpiri (Labrafil M, Tween 80, Tween 20, me te propylene glycol). He tino iti te wairewa o Chx.Hcl i roto i te waikawa oleic, ka puta he kapua ina titrated te waikawa oleic ki te pata tuatahi o te wai. Nō reira, i tangohia te pūnaha waikawa oleic mai i tēnei rangahau. Kua whakaritea ētahi atu whakatakotoranga mā te whakamahi i te ranunga hinu me te surfactant 1:9. He whānuitanga pH me te kaha ionic, nō reira i whiriwhiria ai ēnei surfactants.
He mārama ngā whakatakotoranga katoa i whakaritea engari ko te Pūnaha F2, i te āhua kapua, nō reira i tangohia atu i ngā rangahau aromatawai anō.
Ko te hanganga nanoemulsion tino pai me kaha ki te marara katoa me te tere ina waimeha mā te whakaoho ngawari. I whakaatuhia e ngā hanganga nanoemulsion Chx.HCl he poto ngā wā emulsification, mai i te 1.67 ki te 12.33 hēkona. Ko Tween 80 te wā emulsification poto rawa atu. Ka taea te whakamārama i tēnei mā te kaha whakarewa teitei ake o Tween 80. Ka piki ake te wā emulsification-whaiaro me te piki haere o te kukū o te surfactant, tera pea nā te pikinga o te matotoru o te pūnaha i raro i te mahi a te surfactant.
Ko te rahi o te pata o te emulsion te mea e whakatau ana i te tere me te whānuitanga o te tukunga rongoā. Ko te iti o te rahi o te pata emulsion ka hua ake he wā emulsification poto ake, ā, he nui ake te horahanga o te mata mō te mimiti rongoā. Ko ngā rahi toharite o te pata o ngā hanganga kua tīpakohia o te nanoemulsion Chx.HCl ko 711±0.44, 587±15.3, 10.97±0.11, 16.43±4.55, me te 12.18±2.48, ā, ko te PDI ko 0.76, 0.19, 0.61, 0.47 me te 0.76 mō F1, F2. , F3 me 0.16 ia F4, F5 me F6. Ko ngā hanganga kei roto ko Tween 80 hei surfactant i whakaatu i ngā spherulites iti ake. Tērā pea nā tōna kaha emulsifying teitei ake tēnei. Ko te uara PDI iti ake e tohu ana i te tohatoha rahi pūnaha whaiti ake. He āhua ma te āhua o ēnei whakatakotoranga nā te mea he iti ake ō rātou hihi maturuturu i te roangaru whatu o te mārama e kitea ana (390-750 nm) e iti rawa ai te marara o te mārama. 41
Kei te pikitia 2 te ōrau o te Chx.HCl i tukuna mai i te whakatakotoranga kua whakaritea. Ko te tukunga katoa o te rongoā mai i ngā whakatakotoranga kua whakaritea o te nanoemulsion Chx.HCl i waenga i te 2 ki te 7 meneti. I kitea ko te tere tukunga rongoā teitei rawa atu i whiwhihia i roto i te take o te whakatakotoranga nanoemulsion Chx.HCl F6 (2 meneti), tera pea nā te aroaro o Tween 80, i whakaatu i te taumata teitei ake o te emulsification, ā, ko te nanoemulsion hua. ka whakarato i te horahanga mata nui mō te tukunga rongoā, ka taea ai te whakanui ake i ngā tere tukunga rongoā. I taua wā anō, ko ngā āhuatanga wairewa o te propylene glycol ka taea te wairewa i te nui o ngā surfactants hydrophilic ki roto i te hinu. 40
Kua kitea te tukunga o te Chx.HCl i roto i te vitro he rerekē te raupapa nekeneke, ā, kāore he raupapa nekeneke mārama e whakaatu ana i te tukunga o te rongoā mai i ngā whakatakotoranga nanoemulsion kua whakaritea rerekē. Ko te tukunga nekeneke o ngā rongoā F4 he nekeneke tuatahi, ko te tikanga ka tukuna i runga i te ōwehenga o te nui o te rongoā e toe ana i roto i a rātou. 42 Ko te tukunga nekeneke o ētahi atu rongoā he rite ki te tauira horapa Higuasha, i tohu ko te nui o te rongoā i tukuna he ōwehenga ki te pūtake tapawhā o te rongoā katoa me te wairewatanga o te rongoā i roto i te nanoemulsion. 42
I tukuna ngā whakatakotoranga kua tīpakohia ki ngā momo pumau thermodynamic mā te whakamātautau ahotea mā te whakamahi i ngā huringa whakamatao-wera, te centrifugation me ngā huringa tio-rewa. I kitea ko ngā whakatakotoranga F3 me F4 i whakaatu i te maturuturunga o te rongoā i muri i ngā huringa rewa, ko F1 ia i whakaatu i te matotoru (whakapūmau). I paahitia e F5 me F6 te huringa centrifugation tonu, te whakamātautau whakamahana-whakamatao me te whakamātautau tio-rewa. Ko ngā nanoemulsion he pūnaha pumau thermodynamic i hangaia i ētahi kukū o te hinu, te surfactant me te wai me te kore wehenga wāhanga, te emulsification, te pakaru rānei. Ko te pumau wera te mea e wehewehe ai ngā nanoemulsion mai i ngā emulsion, he pumau kinetic, ā, ka mutu ka wehe ki ngā wāhanga. 19 I whakaatu a F3 i te rahi matūriki nui ake (587 nm) i ētahi atu whakatakotoranga, e whakamārama ana pea i te wehenga wāhanga me te maturuturunga rongoā i roto i ngā whakamātautau pumau thermodynamic. Ko te F4 kei roto ko Tween 80 me te kore he co-surfactant i whakaatu i te maturuturunga rongoā, he tohu pea tēnei mō te hiahia ki te whakamahi i te propylene glycol me te Tween 80 hei whakapai ake i te pumau o ngā whakatakotoranga nanoemulsion. Ko te F1 kei roto ko te Tween 20, kāore he surfactant tāpiri, i whakaatu i te matotorutanga (whakapūpū), arā, he pikinga ake o te matotoru, te kaha rānei o te pūpū nā te kohikohinga o ngā pata.
E whakaatu ana ngā hua pumau i te hiranga o te noho mai o tētahi atu matūriki propylene glycol hei whakanui ake i te marara o ngā matūriki me te aukati i te maturuturunga o te rongoā. Ko te 43 F6 te hanganga pai rawa atu nā te iti o te rahi o te matūriki (12.18 nm), te poto o te wā whakapūtanga (1.67 hēkona) me te tere whakarewanga tere i muri i te 2 meneti. I kitea he pūnaha pumau i te taha thermodynamics/physical, nō reira i whiriwhiria ai mō ētahi atu rangahau.
Kei te piki haere te maha o ngā korenga i muri i te maimoatanga o te awa pakiaka, ko te tikanga he nui ake te tūponotanga o ngā tūroro ki te whakawhanake i ngā mate uaua ake. 44,45 Me tango te kiriata koiora i te wā e patu huakita ana me te whakakī i ngā awa pakiaka. 46,47 Nā te uaua o te pūnaha awa pakiaka, ka uaua ki te tango katoa i ngā awa pakiaka kitakita mā te whakamahi i ngā taputapu me te whakamakuku anake. 48 Ko te whai huatanga o ngā otinga horoi awa pakiaka e whakawhirinaki ana ki te urunga o te wai whakamakuku ki roto i te DT me te roa o te pānga ki ngā kitakita. 49 Nō reira, kua whakamatauria, kua whakamātauria hoki ngā tikanga hou mō te whakamate huakita tino pai. Kāore ngā horoinga tikanga e whakakore katoa i te E. faecalis nā te iti o te urunga o te DT.50.
Ko te kaha horoi toharite o te horoi nanoemulsion he 2001.47 µm2, ā, ko te rahi matūriki toharite o te āwhina horoi he 2609.56 µm. Ko te rerekētanga toharite i waenga i te horoi nanoemulsion me te horoi rahi matūriki noa he 608.09 µm2. He rerekētanga tino nui i kitea i roto i te tatauranga (P<0.001) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa me te (uara-P 0.00052). He rerekētanga tino nui i kitea i roto i te tatauranga (P<0.001) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa me te (uara-P 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частицти наблюсть высокозначимая (P<0,001) разница (значение P 0,00052). He rerekētanga tino hira i kitea i roto i te tatauranga (P<0.001) (uara P 0.00052) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara matūriki noa.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P）000 P<0.00 0.00052）.纳米乳液冲洗剂和正常粒径冲洗剂之间存在统计学上高度显着的差异（P）000 P<0.00 0.00052）. Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически зоченьаца (P<0,0001) (значение P 0,00052). He rerekētanga tino nui i kitea i roto i te tatauranga (P<0.0001) i waenganui i te horoi nanoemulsion me te horoi rahi matūriki noa (uara P 0.00052).I whakaatuhia e te nanoemulsion he rerekētanga tino nui i runga i te tatauranga ki te rahi matūriki noa, e whakaatu ana i te horahanga mata paru toenga iti ake, arā, ko te rauemi nanoemulsion te mea i pai rawa te kaha horoi, e whakaaturia ana i te ahua 3.
Ahua 3. Whakataurite i te mahi horoi o ngā āwhina horoi: (A) me te whakahohenga o te taiaho Nano CHX, (B) me te whakahohenga o te taiaho CHX, (C) me te PUI Nano CHX, (D) me te kore whakahohenga o te Nano CHX, (E) me te kore whakahohenga o te CHX, me te (F)) whakahohenga o te CHX PUI.
Ko te horahanga mata toharite o ngā maramara Chx.HCl 1.6% e toe ana he 2320.36 µm2, ā, ko te horahanga mata toharite o Chx.HCl 2% he 2949.85 µm2. He rerekētanga tino hira i roto i te tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara-P 0.00000). He rerekētanga tino hira i roto i te tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara-P 0.00000). Наблюдалась статистически высокозначимая (P<0,001) растворов и ирригационными растворами с нормальным размером частиц (значение P 0,00000). He rerekētanga tino hira i roto i te tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara P 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P ‼0.00000）.较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学显着的差审学显着的差冲（P0.0（P1 0. Наблюдалась статистически очень значимая разница (P<0,001) между более высокими концентрациями ополасмливательность ополаскивателя с нормальным размером частиц (значение P 0,00000). He rerekētanga tino hira i kitea i roto i te tatauranga (P<0.001) i waenga i ngā kukū teitei ake o te horoi nanoemulsion me te horoi rahi matūriki noa (uara P 0.00000).Ahakoa he iti iho te kukū o te wai whakapūkara nanoemulsion i tērā o te wai whakapūkara rahi matūriki noa, he nui ake te whai huatanga o tēnei kukū iti ki te tango i ngā paru, ā, he nui ake hoki te whai huatanga ki te horoi i ngā awa pakiaka.
He tino rerekētanga nui ā-pūtaiao te PUI (p<0.001) i te whakatauritea ki ētahi atu tikanga whakahohenga. He tino rerekētanga nui ā-pūtaiao te PUI (p<0.001) i te whakatauritea ki ētahi atu tikanga whakahohenga. PUI имел статистически высокозначимую разницу (p<0,001) по сравнению с другими методами активации. He tino rerekētanga nui tā te tatauranga mō te PUI (p<0.001) ki te whakataurite ki ētahi atu tikanga whakahohenga.与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001).与其他激活方法相比，PUI 具有统计学上非常显着的差异(p<0.001). По сравнению с другими методами активации PUI имел статистически очень значимую разницу (p<0,001). Ki te whakatauritea ki ētahi atu tikanga whakahohe, he tino hira te rerekētanga o te PUI i roto i ngā tatauranga (p<0.001).I te whakahohenga o te ISP, ko te horahanga toharite o te mata toenga o ngā para he 1695.31 µm2. Ko te rerekētanga toharite i waenga i te PUI me te Laser he 987.89929 e whakaatu ana i te rerekētanga tino nui o te tatauranga (P<0.001) me te (uara-p 0.00000). Ko te rerekētanga toharite i waenga i te PUI me te Laser he 987.89929 e whakaatu ana i te rerekētanga tino nui o te tatauranga (P<0.001) me te (uara-p 0.00000). Средняя разница между PUI me Laser составила 987,89929, демонстрируя высокостатистически значимую (P<0,001) разпни-0,001 (P<0,001) разпни-0,0ч). Ko te rerekētanga toharite i waenga i te PUI me te Laser he 987.89929, e whakaatu ana i te rerekētanga tino nui o te tatauranga (P<0.001) mai i te (uara-p 0.00000). PUI 和Laser 之间的平均差异为987.89929，显示出高度统计学显着性(P<0.001) 差异(p 000).PUI me te Laser Средняя разница между PUI me Laser составила 987,89929, что свидетельствует о высокой статистической значимости (Pеничимости1)<0,0низыцы (P-0низцы1) 0,00000). Ko te rerekētanga toharite i waenga i te PUI me te Laser he 987.89929, e tohu ana i te rerekētanga hiranga tatauranga teitei (P<0.001) (uara-p 0.00000). Ko te rerekētanga toharite i waenga i te PUI me te kore whakahohenga he 712.40643 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te uara-p o 0.00098). Kāore i tino rerekē te whakamahinga o te whakahohenga Taiaho, te kore whakahohenga rānei (P>0.05) me te uara-P o 0.451211. Ko te rerekētanga toharite i waenga i te PUI me te kore whakahohenga he 712.40643 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te uara-p o 0.00098). he uara-P o 0.451211. Средняя разница между PUI и отсутствием активации составила 712,40643, демонстрируя высокостатистически з (1) чим,0сю p-значением 0,00098). Ko te rerekētanga toharite i waenga i te PUI me te kore whakahohenga he 712.40643, e whakaatu ana i te rerekētanga tino nui (P<0.001) me te uara-p o te 0.00098).Uara-P 0.451211. PUI 和未激活之间的平均差异为712.40643，显示高度统计学显着性差异(P<0.001)，p 0.001)，p 0.001).PUI Средняя разница между PUI и инактивацией составила 712,40643, что свидетельствует о высокой статистической значеской значиской (1,00 p-значение 0,00098). Ko te rerekētanga toharite i waenga i te PUI me te whakakorenga he 712.40643, e tohu ana i te hiranga tatauranga teitei o te rerekētanga (P<0.001, uara-p 0.00098).使用激光激活或不激活在统计学上没有显着差异(P>0.05) P 为0.451211.使用激光激活或不激活在统计学上没有显着差异(P>0.05) P 为0.451211. Статистически значимой разницы (P>0,05) с лазерной активацией или без нее не было со значением P 0,451211. Kāore he rerekētanga nui i kitea i roto i te tatauranga (P>0.05) me te whakahohenga taiaho, me te kore rānei, ko te uara P o te 0.451211.Ko te horahanga mata toharite o ngā kongakonga e toe ana i te wā i whakahohehia ai te laser ko 2683.21 µm2. Ko te horahanga mata toharite o ngā kongakonga e toe ana kāore i whakahohehia ko 2407.72 µm2. Ki te whakatauritea ki te whakahohenga laser, ki te kore rānei i whakahohehia, he iti ake te horahanga mata maramara toharite a te PUI, arā, ko te mana horoi pai rawa atu.
Ko te kaha horoi toharite o te horoi nanoemulsion he 2001.47 µm2, ā, ko te rahi matūriki toharite o te āwhina horoi he 2609.56 µm. Ko te rerekētanga toharite i waenga i te horoi nanoemulsion me te horoi rahi matūriki noa he 608.09 µm2. He rerekētanga tino nui i kitea i roto i ngā tatauranga (P<0.001) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa me te (uara-P 0.00052). He rerekētanga tino nui i kitea i roto i ngā tatauranga (P<0.001) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa me te (uara-P 0.00052). Между ирригационными растворами наноэмульсии и ирригационными растворами с нормальным размером частицтистистила стила высокозначимая (P<0,001) разница (значение P 0,00052). He rerekētanga tino hira i kitea i roto i te tatauranga (P<0.001) (uara P 0.00052) i waenga i ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara matūriki noa.纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P值0.00052）。 P<0.001）（P 0.00052）. Между ополаскивателем с наноэмульсией и ополаскивателем с нормальным размером частиц была статистически зоченьаца (P<0,0001) (значение P 0,00052). He rerekētanga tino nui i kitea i roto i te tatauranga (P<0.0001) i waenganui i te horoi nanoemulsion me te horoi rahi matūriki noa (uara P 0.00052).Ki te whakatauritea ki tētahi rauemi rahi matūriki noa, he tino nui te rerekētanga o te nanoemulsion i roto i ngā tatauranga, e whakaatu ana i te iti ake o te horahanga mata o ngā paru toenga, arā, he pai ake te kaha horoi o te rauemi Nanoemulsion e whakaaturia ana i te Pikitia 3.
Ko te horahanga mata toharite o ngā maramara Chx.HCl 1.6% e toe ana he 2320.36 µm2, ā, ko te horahanga mata toharite o Chx.HCl 2% he 2949.85 µm2. He rerekētanga tino nui i roto i ngā tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara-P 0.00000). He rerekētanga tino hira i roto i te tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara-P 0.00000). Имелась статистически высокодостоверная (P<0,001) средств и ирригационными растворами с нормальным размером частиц (значение P 0,00000). He rerekētanga nui ā-tatauranga (P<0.001) i waenga i te kukū teitei ake o ngā wai whakapūkara nanoemulsion me ngā wai whakapūkara rahi matūriki noa (uara P 0.00000).较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P值0.00000）。较高浓度的纳米乳液冲洗剂与正常粒径冲洗剂之间存在统计学上高度显着的差异（P<0.001）（P000 Наблюдалась статистически высокозначимая разница (P <0,001) между более высокими концентрациями ополасмливательность ополаскивателем с нормальным размером частиц (значение P 0,00000). He rerekētanga tino hira i kitea i roto i ngā tatauranga (P < 0.001) i waenga i ngā kukū teitei ake o te horoi nanoemulsion me te horoi rahi matūriki noa (uara P 0.00000).Ahakoa he iti iho te kukū o te wai whakapūkara nanoemulsion i tērā o te wai whakapūkara rahi matūriki noa, he nui ake te whai huatanga o tēnei kukū iti ki te tango i ngā paru, ā, he nui ake hoki te whai huatanga ki te horoi i ngā awa pakiaka.
He nui te rerekētanga nui o te PUI i runga i ngā tatauranga (p<0.001) i te whakatauritea ki ētahi atu tikanga whakahohenga. He nui te rerekētanga nui o te PUI i runga i ngā tatauranga (p<0.001) i te whakatauritea ki ētahi atu tikanga whakahohenga. PUI имел статистически высокую значимую разницу (p<0,001) по сравнению с другими методами активации. He rerekētanga nui ā-tatauranga tō te PUI (p<0.001) ki ērā atu tikanga whakahohenga.与其他激活方法相比，PUI 具有统计学上的显着差异(p<0.001). Ki te whakatauritea ki ētahi atu tikanga whakahohe, he rerekētanga nui tā te tatauranga mō te PUI (p<0.001). PUI статистически значимо отличался (p<0,001) по сравнению с другими методами активации. He rerekētanga nui ā-tatauranga te PUI (p<0.001) ki ērā atu tikanga whakahohenga.I te wā i whakahohehia ai te PUI, ko te horahanga toharite o ngā para mata e toe ana he 1695.31 μm2. Ko te rerekētanga toharite i waenga i te PUI me te Laser he 987.89929 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te (uara-p 0.00000). Ko te rerekētanga toharite i waenga i te PUI me te kore whakahohenga he 712.40643 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te (uara-p 0.00098). Kāore he rerekētanga tatauranga nui (P>0.05) o te whakamahinga o te whakahohenga Laser, te kore whakahohenga rānei ki te (uara-P 0.451211). Ko te rerekētanga toharite i waenga i te PUI me te Taiaho he 987.89929 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te (uara-p 0.00000). Ko te rerekētanga toharite i waenga i te PUI me te kore whakahohenga he 712.40643 e whakaatu ana i te rerekētanga tino nui (P<0.001) me te (uara-p 0.00098). Kāore he rerekētanga nui (P>0.05) i waenga i te whakamahinga o te whakahohenga Taiaho, te kore whakahohenga rānei me te (uara-P 0.451211). Средняя разница между PUI и лазером составила 987,89929, демонстрируя высокостатистически значимуцю (P<0,001) снич (P<0,001) снич 0,00000). Ko te rerekētanga toharite i waenga i te PUI me te taiaho he 987.89929, e whakaatu ana i te rerekētanga tino hiranga ā-tatauranga (P<0.001) me te (uara-p 0.00000). - значение 0,00098) . (P-значение 0,451211). - uara 0.00098). He rerekētanga nui te whakamahinga o te whakahohenga taiaho, te kore whakahohenga rānei (P>0.05) ki te (uara-P 0.451211). PUI 和激光之间的平均差异为987.89929，与(p ‼0.00000) 差异具有高度统计学意(P)<0.00(P). Ko te rerekētanga toharite i waenga i te PUI me te taiaho he 987.89929, ā, he nui te hiranga tatauranga o te rerekētanga (p 值0.00000) (P<0.001). Средняя разница между PUI и лазером составила 987,89929, что было высоко статистически значимым (P<0,001) с (з0,ч) с (з0,ч). Ko te rerekētanga toharite i waenga i te PUI me te taiaho he 987.89929, he mea tino hirahira i te tatauranga (P<0.001) me te (uara p 0.00000). PUI 与未激活之间的平均差异为712.40643，与(p) 差异具有高度统计学意义(P<0.001) -0.001). Ko te rerekētanga toharite i waenga i te PUI me te kore mahi he 712.40643, ā, he nui te hiranga tatauranga o te rerekētanga (p) (P<0.001) – te uara 0.00098. Средняя разница между PUI и инактивацией составила 712,40643, что было высоко статистически значимым с разила (1<0,0ч) 0,00098). Ko te rerekētanga toharite i waenga i te PUI me te whakakorenga he 712.40643, he mea tino nui te hiranga o te tatauranga me te rerekētanga (p) (P<0.001 – uara 0.00098).使用激光激活或不激活没有显着统计学差异(P>0.05) 与(P 0.451211). Kāore he rerekētanga tatauranga nui i waenga i te whakahohenga taiaho me te kore whakahohenga (P>0.05) me (P 值0.451211). Не было статистически значимой разницы (P>0,05) по сравнению с (значение P 0,451211) с лазерной активацией или б. Kāore he rerekētanga nui i kitea i roto i te tatauranga (P>0.05) ki te whakataurite ki (uara P 0.451211) me te whakahohenga taiaho, me te kore rānei.Ko te horahanga mata toharite o ngā kongakonga e toe ana i te wā whakahohenga laser ko 2683.21 μm2. Ko te horahanga mata toharite o ngā kongakonga e toe ana kāore i whakahohehia ko 2407.72 μm2. Ki te whakatauritea ki te whakahohenga laser, ki te kore rānei e whakahohehia, he iti ake te horahanga mata toharite o te maramara a te PUI, arā, he pai ake te kaha horoi.
Ko te pānga toharite o te horoi nanoemulsion ki te tango paru he nui ake i te tikanga tatauranga i tērā o te horoi rahi matūriki noa. Chx.HCl 1.6%, PUI 1938.77 µm2, 2510.96 µm2 me te taiaho. Ki te kore he whakahohenga, ko te uara toharite he 2511.34 µm2. I te whakamahinga o te 2% Chx.HCl me te whakahohenga ki te taiaho, ko ngā hua te kino rawa atu, ā, ko te nui rawa o ngā paru te nui rawa atu. I puta ngā hua ōrite i te korenga o te 0.75% Chx.HCl i whakahohea. He mārama, ko ngā hua pai rawa atu i puta mā te whakamahi i ngā kukū teitei ake o te āwhina horoi i roto i te nanoemulsion. Ko te PUI te mea tino whai hua i roto i te whakahohenga whakamakuku me te horoi paru, e whakaaturia ana i te Pikitia 3A-F)).
E ai ki te Ripanga 2, he pai ake te mahi a te nanoemulsion Chx.HCl i ngā matūriki rahi noa e pā ana ki te maha o ngā moroiti ora, ā, he hononga pai ki te urunga o te hanganga me te pānga horoi e ai ki ngā tawhā e whai ake nei: te rahi, te kukū o te kaihoko horoi me te tikanga whakahohe.
Ka taea te whakangaro katoa i ngā huakita mā te whakamahi i te kukū teitei ake o te āwhina horoi. Ahakoa te whakahohenga PUI, ko te 0.75% Chx.HCl te mea kino rawa atu te pānga patu huakita. He pānga kino te whakahohenga taiaho ki ngā horoi nano-emulsion. E kitea ana i ngā hua o mua, ka whakaitihia e te whakamahinga o te taiaho te whai huatanga o te nanoemulsion Chx.HCl 0.75%, ko te CFU o te nanoChx.HCl 0.75% he 195, he uara tino teitei, e tohu ana he rite ngā tauhohenga i tēnei kukū ki te whakahohenga taiaho. He photothermal ngā taiaho diode, nō reira ka taea e te mārama, e te wera rānei te mea ka ngaro te pānga patu huakita o te nanoemulsion. Ko te hua o ngā kukū teitei ko te whakangaromanga katoa o ngā huakita. I whakaatu te Nano Chx.HCl 1.6% i te tipu huakita kino i te aroaro o te whakahohenga taiaho, ko te tikanga kāore te taiaho i pā ki te kaha patu huakita o te nano Chx.HCl 1.6%. Ka taea te whakatau ko te rauemi nanoemulsion me te kukū teitei ake he pānga patu huakita pai ake.
I roto i tēnei mahi, i whakaritea ngā nanoemulsion Chx.HCl mā te whakamahi i ngā hinu rerekē e rua, arā, ngā surfactants e rua me te co-surfactant, ā, i whiriwhiria te whakatakotoranga tino pai (F6) me te rahi matūriki iti, te wā emulsification poto me te tere whakarewatanga teitei). Hei tāpiri, i whakamatautauria te (F6) mō te pumau thermodynamic/physical. I roto i te nanoemulsion Chx.HCl i te kukū o te 1.6%, i whakaatu te nanoemulsion Chx.HCl i te urunga pai rawa atu i roto i ngā ngongo niho i whakaritea ki te Chx.HCl tuku iho hei wai horoi, ā, he kaha horoi te PUI hei tikanga whakahohe. Hei tāpiri, i whakaatu ngā rangahau antibacterial o te nanoemulsion Chx.HCl i te whakakorenga katoa o ngā huakita. I whakaūhia tēnei e ngā hua. Ka taea te whakaaro ko te nanoemulsion Chx.HCl he wai horoi whai hua.
He mihi nui ki ngā kaimahi o te taiwhanga rangahau o te Whare Wānanga Pūtaiao me te Hangarau o Misr mō tā rātou tautoko nui.