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Gukurikirana isesengura ryamazi afite ibintu byinshi byifashishwa mubumenyi bwubuzima no gukurikirana ibidukikije. Muri iki gikorwa, twateje imbere fotometeri yoroheje kandi ihendutse ishingiye ku cyuma cyitwa waveguide capillaries (MCCs) kugirango tumenye ultrasensitive yo kwinjirira. Inzira ya optique irashobora kwiyongera cyane, kandi ndende cyane kurenza uburebure bwumubiri bwa MWC, kubera ko urumuri rwatatanye kuruhande rwicyuma cyoroshye rushobora kuba ruri muri capillary utitaye kumpande zibyabaye. Kwishyira hamwe nkibice 5.12 nM birashobora kugerwaho ukoresheje reagent zisanzwe za chromogeneque bitewe nuburyo bushya butari umurongo wa optique amplification hamwe no guhinduranya byihuse hamwe na glucose.
Photometrie ikoreshwa cyane mugusesengura ibyitegererezo byamazi bitewe nubwinshi bwibintu bya chromogenic biboneka hamwe na semiconductor optoelectronic ibikoresho1,2,3,4,5. Ugereranije na cuvette gakondo ishingiye ku kwinjirira, capillaries yamazi (LWC) yerekana (TIR) ​​mugukomeza urumuri rwimbere muri capillary1,2,3,4,5. Ariko, nta yandi mananiza, inzira ya optique yegereye gusa uburebure bwumubiri bwa LWC3.6, kandi kongera uburebure bwa LWC burenze m 1.0 bizagira ikibazo cyumucyo mwinshi hamwe ningaruka nyinshi ziterwa n’ibibyimba, nibindi.3, 7. Kubireba selile yatanzwe kugirango iteze imbere inzira nziza, imipaka yo gutahura iratera imbere gusa kubintu 2.5-8.9.
Kuri ubu hari ubwoko bubiri bwingenzi bwa LWC, aribwo capillaries ya Teflon AF (ifite indangagaciro yo kugabanuka ya ~ 1.3 gusa, iri munsi y’amazi) hamwe na silika capillaries yashizwemo na Teflon AF cyangwa firime yicyuma1,3,4. Kugirango ugere kuri TIR kuri interineti hagati yibikoresho bya dielectric, ibikoresho bifite indangantege nkeya kandi bingana n’umucyo mwinshi birasabwa3,6,10. Kubijyanye na capillaries ya Teflon AF, Teflon AF irahumeka kubera imiterere yayo3,11 kandi irashobora gukuramo ibintu bike mubitegererezo by'amazi. Kuri capillaries ya quartz yometse hanze hamwe na Teflon AF cyangwa ibyuma, indangagaciro yo kwangirika ya quartz (1.45) irarenze ibyitegererezo byinshi byamazi (urugero 1.33 kumazi) 3,6,12,13. Kuri capillaries zometseho firime yicyuma imbere, imitungo yo gutwara abantu yarigishijwe 14,15,16,17,18, ariko inzira yo gutwikira iragoye, hejuru ya firime yicyuma ifite imiterere itoroshye kandi yuzuye4,19.
Byongeye kandi, ubucuruzi bwa LWCs (AF Teflon Coated Capillaries na AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) zifite izindi ngaruka mbi, nka: kubera amakosa. . Umubare munini wapfuye wa TIR3,10, (2) T-umuhuza (guhuza capillaries, fibre, na inlet / outlet tubes) irashobora gutega imyuka myinshi10.
Muri icyo gihe, kumenya urugero rwa glucose ni ingenzi cyane mu gusuzuma diyabete, cirrhose y'umwijima n'indwara zo mu mutwe20. nuburyo bwinshi bwo gutahura nka Photometrie (harimo na spekitifotometrike 21, 22, 23, 24, 25 na colimetry kumpapuro 26, 27, 28), galvanometrie 29, 30, 31, fluorometrie 32, 33, 34, 35, optique polarimetry 36, resonance yubutaka. 37, Fabry-Perot cavity 38, amashanyarazi 39 na capillary electrophoresis 40,41 nibindi. Nyamara, bumwe murubwo buryo busaba ibikoresho bihenze, kandi kumenya glucose yibitekerezo bya nanomolar bikomeje kuba ingorabahizi (urugero, kubipimo bifotora21, 22, 23, 24, 25, 26, 27, 28, glucose yibanze cyane). imbogamizi yari 30 nM gusa mugihe Prussian yubururu nanoparticles yakoreshejwe nka mimics ya peroxidase). Isesengura rya Nanomolar glucose risabwa kenshi kubushakashatsi bwa selile yo mu rwego rwa selile nko kubuza gukura kwa kanseri ya prostate y'abantu42 hamwe n'imyitwarire ya CO2 yo gutunganya Prochlorococcus mu nyanja.
Muri iyi ngingo, fotometeri yoroheje, ihendutse ishingiye ku cyuma cyitwa capillary cyuma (MWC), capillary ya SUS316L idafite umuyonga hamwe n’imbere y’amashanyarazi, yakozwe kugirango hamenyekane ultrasensitive absorption. Kubera ko urumuri rushobora gufatirwa mu cyuma cya capillaries hatitawe ku mpande zanduye, inzira ya optique irashobora kwiyongera cyane bitewe no gukwirakwiza urumuri hejuru yicyuma cyoroshye kandi cyoroshye, kandi ni kirekire cyane kuruta uburebure bwumubiri bwa MWC. Mubyongeyeho, T-umuhuza woroheje washyizweho kugirango uhuze optique hamwe na fluid inlet / outlet kugirango ugabanye urugero rwapfuye kandi wirinde kwinjirira. Kuri santimetero 7 MWC ya MWC, igipimo cyo gutahura cyongerewe inshuro zigera ku 3000 ugereranije n’ubucuruzi bwa spropropotometometero yubucuruzi hamwe na cm 1 cuvette bitewe no kongera imbaraga mu nzira idafite umurongo wa optique no guhinduranya byihuse, kandi kwibanda kuri glucose bishobora no kugerwaho. 5.12 nM gusa ukoresheje chromogenic isanzwe.
Nkuko bigaragara ku gishushanyo cya 1, Photometer ishingiye kuri MWC igizwe na cm 7 z'uburebure bwa MWC hamwe na EP yo mu rwego rwa EP ifite amashanyarazi imbere, 505 nm LED ifite lens, fotodetekeri yunguka ishobora guhinduka, na bibiri byo guhuza optique no kwinjiza amazi. Sohoka. Inzira yinzira eshatu ihujwe na Pike inlet tube ikoreshwa muguhindura icyitegererezo cyinjira. Umuyoboro wa Peek uhuye neza na plaque ya quartz na MWC, bityo ingano yapfuye muri T-umuhuza ikabikwa byibuze, bikarinda neza ibyuka bihumeka. Mubyongeyeho, urumuri rwegeranijwe rushobora kwinjizwa byoroshye kandi neza muri MWC binyuze muri T-piece quartz.
Urugero rw'ibiti n'amazi byinjijwe muri MCC binyuze muri T-agace, kandi urumuri runyura muri MCC rwakirwa na fotodetector. Ibisubizo byinjira byintangarugero cyangwa ubusa byinjijwe muburyo bwa ICC binyuze mumurongo winzira eshatu. Ukurikije amategeko ya Byeri, ubwinshi bwa optique bwurugero rwamabara burashobora kubarwa uhereye kuburinganire. 1.10
aho Vcolor na Vblank nibimenyetso bisohoka bya fotodetekeri mugihe ibara hamwe nicyitegererezo cyambaye ubusa byinjijwe muri MCC, naho Vdark nikimenyetso cyerekana inyuma ya fotodetector mugihe LED yazimye. Guhindura mubisohoka ibimenyetso ΔV = Ibara - Vblank birashobora gupimwa muguhindura ingero. Ukurikije ikigereranyo. Nkuko bigaragara ku gishushanyo 1, niba ΔV ari nto cyane kurenza Vblank - Vdark, mugihe ukoresheje gahunda yo guhinduranya icyitegererezo, impinduka nto muri Vblank (urugero drift) zirashobora kugira ingaruka nke kubiciro bya AMWC.
Kugereranya imikorere ya MWC ishingiye kuri MWC hamwe na cuvette ishingiye kuri spekitifotometero, igisubizo cya wino itukura cyakoreshejwe nkicyitegererezo cyamabara kubera amabara meza meza kandi gihamye, DI H2O nkicyitegererezo cyuzuye. . Nkuko bigaragara mu mbonerahamwe ya 1, urukurikirane rwibisubizo bitukura byateguwe nuburyo bukurikirana bwo gukoresha DI H2O nkibisubizo. Ubwinshi bwikigereranyo cya sample 1 (S1), irangi ritukura ritavanze, ryagenwe nka 1.0. Ku mutini. Igishushanyo cya 2 cyerekana amafoto ya optique yerekana ingero 11 za wino zitukura (S4 kugeza S14) hamwe nubushakashatsi bugereranijwe (bwerekanwe kumeza 1) kuva kuri 8.0 × 10–3 (ibumoso) kugeza 8.2 × 10-10 (iburyo).
Ibisubizo byo gupima kuri sample 6 byerekanwe mubishushanyo. 3 (a). Ingingo zo guhinduranya hagati yintangarugero nubusa byerekanwe mubishushanyo n'imyambi ibiri “↔”. Birashobora kugaragara ko ibisohoka voltage yiyongera byihuse mugihe uhinduye ibara ryamabara ujya kubusa kandi nibindi. Vcolor, Vblank hamwe na ΔV bihuye birashobora kuboneka nkuko bigaragara ku gishushanyo.
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Ibisubizo byo gupima kuburugero 9, 13, na 14 byerekanwe mubishushanyo. 3 (b) - (d), kimwe. Nkuko bigaragara ku gishushanyo cya 3 (d), ΔV yapimwe ni 5 nV gusa, ikubye hafi inshuro 3 agaciro k urusaku (2 nV). Gitoya ΔV biragoye gutandukanya urusaku. Rero, imipaka yo gutahura yageze ku gipimo cya 8.2 × 10-10 (icyitegererezo 14). Hamwe nuburinganire. 1. Kwinjira kwa AMWC birashobora kubarwa uhereye kuri Vcolor yapimwe, Vblank na Vdark. Kuri Photodetector yungutse 104 Vdark ni -0,68 μV. Ibisubizo byo gupima kuburugero byose byavuzwe mu mbonerahamwe ya 1 kandi urashobora kubisanga mubikoresho byiyongera. Nkuko bigaragara mu mbonerahamwe ya 1, iyinjizwa ryabonetse ryinshi ryinshi, bityo rero kwinjiza hejuru ya 3.7 ntibishobora gupimwa na MWC ishingiye kuri spekrometero.
Kugereranya, icyitegererezo cya wino itukura nacyo cyapimwe hamwe na spekitifotometero kandi igipimo cya Acuvette cyapimwe cyerekanwe ku gishushanyo cya 4. Indangagaciro za Acuvette kuri 505 nm (nkuko bigaragara mu mbonerahamwe ya 1) zabonetse hifashishijwe imirongo y'icyitegererezo 10, 11, cyangwa 12 (nkuko bigaragara mu nsobanuro). Kuri Ishusho 4) nk'ibanze. Nkuko byerekanwe, igipimo cyo gutahura cyageze ku gipimo cya 2.56 x 10-6 (icyitegererezo 9) kubera ko umurongo wo kwinjiza ingero 10, 11 na 12 utari utandukanye. Rero, mugihe ukoresheje MWC ishingiye kuri MWC, igipimo cyo gutahura cyatejwe imbere nikintu 3125 ugereranije na cuvette ishingiye kuri spekitifoto.
Kwishingira kwishira-kwibanda ku gishushanyo.5. Kubipimo bya cuvette, iyinjizwa iringaniza na wino yibanda kumuhanda uburebure bwa cm 1. Mugihe, kubipimo bishingiye kuri MWC, kwiyongera kutari kumurongo kwiyongera kwakorewe byagaragaye cyane. Dukurikije amategeko ya Byeri, kwinjiza bigereranywa nuburebure bwinzira ya optique, bityo kwinjiza inyungu AEF (bisobanurwa ngo AEF = AMWC / Acuvette kumurongo umwe wino) ni igipimo cya MWC nuburebure bwinzira ya cuvette. Nkuko bigaragara ku gishushanyo cya 5, hejuru cyane, AEF ihoraho ni hafi 7.0, ibyo birumvikana kuko uburebure bwa MWC bwikubye inshuro 7 uburebure bwa cm 1 cuvette. Nyamara, kuri concentration nkeya (kwibandaho bifitanye isano <1.28 × 10-5), AEF yiyongera hamwe no kugabanuka kwinshi kandi yagera ku gaciro ka 803 kuri concentration ya 8.2 × 10-10 mugukuramo umurongo wo gupima cuvette. Nyamara, kuri concentration nkeya (kwibandaho bifitanye isano <1.28 × 10-5), AEF yiyongera hamwe no kugabanuka kwinshi kandi yagera ku gaciro ka 803 kuri concentration ya 8.2 × 10-10 mugukuramo umurongo wo gupima cuvette. Однако при низких кирхх (относительная) баш <1,28 × 10–5) AEF увеличивается с уменьшением профессионрации и может достиит значения 803 при относительной чакрации 8,2 × 10-10 Nyamara, kuri concentration nke (ugereranije <1.28 × 10–5), AEF yiyongera hamwe no kugabanuka kwinshi kandi irashobora kugera ku gaciro ka 803 ugereranije na 8.2 × 10-10 iyo ikuwe mubitereko bishingiye kuri cuvette.然而，在低浓度（相关浓度 <1.28 × 10-5 ）下， AEF 随着浓度的降低而增加，并且通过外推基于比色皿的测量曲线，在相关浓度为 8.2 × 10-10 时将达到 803 的值。然而 ， 在 低 浓度 （相关 浓度 <1.28 × 10-5） ， ， AEF 随着 的 降低 而 ， 并且 通过 外推 为 为 8.2 × 10-10 时 达到 达到 达到 Однако при низких барных (релевантные срации <1,28 × 10-5) АЭП увеличивается с уменьшением пррации, и при экстраполяции кривой измерения на основе кюветы она Nyamara, iyo yibanze cyane (kwibanda kuri <1.28 × 10-5) AED yiyongera hamwe no kugabanuka kwinshi, kandi iyo ikuwe mubisumizi bishingiye ku cuvette ishingiye ku gupima, igera ku gaciro kangana na 8.2 × 10-10 803.Ibi bivamo inzira ihuye ya cm 803 (AEF × 1 cm), ikaba ndende cyane kuruta uburebure bwumubiri bwa MWC, ndetse ikaba ndende kuruta LWC ndende cyane mubucuruzi (cm 500 uvuye World Precision Instruments, Inc.). Doko Engineering LLC ifite uburebure bwa cm 200). Uku kwiyongera kutari kumurongo mukwinjira muri LWC ntabwo byigeze bivugwa.
Ku mutini. 6 (a) - (c) herekana ishusho ya optique, ishusho ya microscope, hamwe na optique yerekana ishusho yubuso bwimbere bwigice cya MWC. Nkuko bigaragara ku gishushanyo. 6 (a), ubuso bwimbere buroroshye kandi burabagirana, burashobora kwerekana urumuri rugaragara, kandi rugaragaza cyane. Nkuko bigaragara ku gishushanyo. 6 (b), kubera guhindagurika hamwe na kristaline imiterere yicyuma, mesa ntoya nibitagenda neza bigaragara neza. Urebye agace gato (<5 μm × 5 μ m), ubukana bwubuso bwinshi buri munsi ya 1,2 nm (Ishusho 6 (c)). Urebye agace gato (<5 μ m × 5 μ m), ubukana bwubuso bwinshi buri munsi ya 1,2 nm (Ishusho 6 (c)). Вивуу малой площ ради (<5 мкм × 5 мкм) шероховатость большей части поверхности соверляет мнее 1,2 нм (рис. 6 (в)). Bitewe n'akarere gato (<5 µm × 5 µm), ubukana bwa hafi yubuso buri munsi ya 1,2 nm (Ishusho 6 (c)).考虑到小面积（ <5 μ m × 5 μm ），大多数表面的粗糙度小于 1.2 nm （图 6 （c ））。考虑到小面积（ <5 μ m × 5 μm ），大多数表面的粗糙度小于 1.2 nm （图 6 （c ））。 Учитывая небольшую площадь (<5 мкм × 5 мкм), шероховатость большинства поверхностей соверляет мнее 1,2 нм (рис. 6 (в)). Urebye agace gato (<5 µm × 5 µm), ubukana bwibibanza byinshi ntiburi munsi ya 1,2 nm (Ishusho 6 (c)).
(a) Ishusho nziza, (b) ishusho ya microscope, na (c) ishusho nziza yubuso bwimbere bwa MWC yaciwe.
Nkuko bigaragara ku gishushanyo. 7 (a), inzira optique LOP muri capillary igenwa nu mpande zanduye θ (LOP = LC / sinθ, aho LC nuburebure bwumubiri bwa capillary). Kuri capillaries ya Teflon AF yuzuyemo DI H2O, inguni yibyabaye igomba kuba irenze inguni ya 77.8 °, bityo LOP ikaba iri munsi ya 1.02 × LC nta yandi mananiza3.6. Mugihe, hamwe na MWC, gufunga urumuri imbere muri capillary ntaho bitaniye nigipimo cyangirika cyangwa inguni yibyabaye, kuburyo rero impande zifatika zigabanuka, inzira yumucyo irashobora kuba ndende cyane kurenza uburebure bwa capillary (LOP »LC). Nkuko bigaragara ku gishushanyo. 7 (b), hejuru yicyuma gishobora gukurura urumuri, rushobora kongera inzira nziza.
Kubwibyo, hari inzira ebyiri zumucyo kuri MWC: urumuri rutaziguye rutagaragajwe (LOP = LC) nurumuri rwibiti hamwe nibitekerezo byinshi hagati yinkuta zuruhande (LOP »LC). Dukurikije amategeko ya Byeri, ubukana bwurumuri rwanduye rwerekanwa na zigzag rushobora kugaragazwa nka PS × exp (-α × LC) na PZ × exp (-α × LOP), aho buri gihe α ari coeffisente yo kwinjiza, biterwa ahanini nubushakashatsi bwa wino.
Kuri wino yibanda cyane (urugero, kwibandaho bifitanye isano> 1.28 × 10-5), zigzag-urumuri rwiyegereye cyane kandi ubukana bwarwo buri munsi cyane yumucyo ugororotse, bitewe na coefficient nini yo kwinjirira hamwe ninzira ndende ya optique-inzira. Kuri wino yibanda cyane (urugero, kwibandaho bifitanye isano> 1.28 × 10-5), zigzag-itara ryiyegereye cyane kandi ubukana bwaryo buri munsi cyane yumucyo ugororotse, bitewe na coefficient nini yo kwinjirira hamwe ninzira ndende ndende. Для чернил с высокой крациций (например, относительная зира> 1,28 × 10-5) зигзагооб башный свет силно захает, а его ин интснность намного ниже, чем у прямого света, из-за баччог. длинного оптического излучения. Kuri wino yibanda cyane (urugero: kwibanda kuri 1.28 × 10-5), urumuri rwa zigzag ruba rwinshi kandi ubukana bwarwo buri munsi cyane yumucyo utaziguye bitewe na coeffisente nini yo kwinjirira kandi ikarekura cyane.inzira.对于高浓度墨水（例如，相关浓度> 1.28 × 10-5 ）， Z 字形光衰减很大，其强度远低于直光，这是由于吸收系数大，光学时间更长。对于 高浓度 墨水 （例如 ， 浓度 浓度> 1.28 × 10-5） ， z 字形 衰减 很 大 ， 强度 是 直光 大 这 时间 更。。。 长 长 长 长 长 长 长 长 长Для чернил с высокой крациций (например, релевантные шрации> 1,28 × 10-5) зигзагооб башный свет значительно ослабляется, и его интвенсность намного няже, чем у прямого согч? boлее длительного оптического времени. Kuri wino yibanda cyane (urugero, kwibandaho> 1.28 × 10-5), urumuri rwa zigzag rwarushijeho kwiyongera kandi ubukana bwarwo buri munsi cyane yumucyo utaziguye bitewe na coefficient nini yo kwinjirira kandi igihe kinini cyo guhitamo.umuhanda muto.Rero, urumuri rutaziguye rwiganjemo kugena (LOP = LC) kandi AEF yagumishijwe kuri ~ 7.0. Ibinyuranye, iyo kwinjiza-coefficient igabanutse hamwe no kugabanuka kwa wino (urugero, kwibandaho bifitanye isano <1.28 × 10-5), ubukana bwumucyo wa zigzag bwiyongera byihuse kuruta ubw'umucyo ugororotse hanyuma zigzag-urumuri rutangira kugira uruhare runini. Ibinyuranye, iyo kwinjiza-coefficient igabanutse hamwe no kugabanuka kwa wino (urugero, kwibandaho bifitanye isano <1.28 × 10-5), ubukana bwumucyo wa zigzag bwiyongera byihuse kuruta ubw'umucyo ugororotse hanyuma zigzag-urumuri rutangira kugira uruhare runini. Напротив, когда коэфффентент поглощения уменьшается с уменьшением мракрации чернил (наприма, относительная матри <1,28 × 10-5), кининает играть зигзагооб Канный свет. Ibinyuranye na byo, iyo coeffisiyoneri yo kugabanuka igabanutse hamwe no kugabanuka kwa wino (urugero, igereranya ryagereranijwe <1.28 × 10-5), ubukana bwurumuri rwa zigzag bwiyongera vuba kurenza urumuri rutaziguye, hanyuma urumuri rwa zigzag rutangira gukina.uruhare runini.相反，当吸收系数随着墨水浓度的降低而降低时（例如，相关浓度 <1.28 × 10-5 ）， Z 字形光的强度比直光增加得更快，然后 Z 字形光开始发挥作用一个更重要的角色。相反 ， 当 吸收 系数 墨水 1. 1. 1. 1. 1. 1. <1.28 × 10-5） ， 字形光 的 然后 然后 字形光 然后 更 更 I 更 更 更 更 I. зигзагооб Канный свет Кинаина играть более важную бар. Ibinyuranye, iyo coeffisente yo kwinjiza igabanutse hamwe no kugabanuka kwa wino (urugero, kwibanda kuri <1.28 × 10-5), ubukana bwurumuri rwa zigzag bwiyongera vuba kurenza urumuri rutaziguye, hanyuma urumuri rwa zigzag rutangira kugira uruhare runini.imiterere y'uruhare.Kubwibyo, kubera inzira ya optique yinzira (LOP »LC), AEF irashobora kwiyongera kurenza 7.0. Ibiranga urumuri rwiza rwa MWC urashobora kuboneka ukoresheje uburyo bwa waveguide.
Usibye kunoza inzira ya optique, kwihuta kwicyitegererezo nabyo bigira uruhare mumipaka ya ultra-low detection. Bitewe nubunini buke bwa MCC (0,16 ml), igihe gikenewe cyo guhindura no guhindura ibisubizo muri MCC gishobora kuba munsi yamasegonda 20. Nkuko bigaragara ku gishushanyo cya 5, agaciro ntarengwa kamenyekana ka AMWC (2.5 × 10–4) kari munsi yinshuro 4 ugereranije na Acuvette (1.0 × 10–3). Guhindura byihuse igisubizo gitemba muri capillary bigabanya ingaruka zurusaku rwa sisitemu (urugero drift) kumurongo wukuri gutandukanya itandukaniro ugereranije nigisubizo cyo kugumana muri cuvette. Kurugero, nkuko bigaragara mumitini. 3.
Nkuko bigaragara mu mbonerahamwe ya 2, urutonde rwibisubizo bya glucose mubitekerezo bitandukanye byateguwe hifashishijwe DI H2O nkibisubizo. Ingero zisize cyangwa zateguwe zivanze no kuvanga glucose cyangwa amazi ya deionised hamwe na chromogenic ibisubizo bya glucose oxydease (IMANA) na peroxidase (POD) 37 mubipimo byagenwe bya 3: 1. Ku mutini. 8 yerekana amafoto ya optique yicyitegererezo cyenda (S2-S10) hamwe na glucose yibanze kuva kuri mM 2.0 (ibumoso) kugeza 5.12 nM (iburyo). Umutuku ugabanuka hamwe no kugabanuka kwa glucose.
Ibisubizo byo gupima ingero 4, 9, na 10 hamwe na MWC ishingiye kuri Photometer irerekanwa mumashusho. 9 (a) - (c), kimwe. Nkuko bigaragara ku gishushanyo. 9. Rero, ibipimo bikurikirana ΔV ntibishobora gusubirwamo kuburugero hamwe na glucose yibanze kuri munsi ya 5.12 nM (sample 10), kuko iyo ΔV ari nto bihagije, ihungabana ryimana-POD reagent ntirishobora kwirengagizwa. Kubwibyo, imipaka yo kumenya igisubizo cya glucose ni 5.12 nM, nubwo agaciro ka ΔV (0.52 µV) nini cyane kuruta agaciro k urusaku (0.03 µV), byerekana ko ΔV ntoya ishobora kuboneka. Iyi mipaka yo gutahura irashobora kurushaho kunozwa ukoresheje reagent zihamye za chromogenic.
(a) Ibisubizo byo gupima icyitegererezo cya 4, (b) icyitegererezo cya 9, na (c) icyitegererezo cya 10 ukoresheje Photometer ishingiye kuri MWC.
Kwinjira kwa AMWC birashobora kubarwa ukoresheje indangagaciro zapimwe Vcolor, Vblank na Vdark. Kuri Photodetector hamwe ninyungu 105 Vdark ni -0.068 μV. Ibipimo by'icyitegererezo byose birashobora gushyirwaho mubikoresho byiyongera. Kugereranya, ingero za glucose nazo zapimwe hamwe na spekitifotometero kandi ibipimo byapimwe bya Acuvette byageze ku gipimo cya 0,64 µM (icyitegererezo 7) nkuko bigaragara ku gishushanyo cya 10.
Isano iri hagati yo kwishira hamwe no kwibanda ku gishushanyo cya 11. Hamwe na Photometer ishingiye kuri MWC, iterambere ryikubye inshuro 125 kugipimo cyo gutahura ryagezweho ugereranije na cuvette ishingiye kuri spekitifoto. Iri terambere riri munsi ya wino itukura bitewe nubushake buke bwimana-POD reagent. Ubwiyongere butari umurongo bwo kwinjirira mukwibanda buke nabwo bwagaragaye.
Ifoto ya MWC yakozwe kugirango ikoreshwe cyane kugirango ibone urugero rwamazi. Inzira ya optique irashobora kwiyongera cyane, kandi ndende cyane kurenza uburebure bwumubiri bwa MWC, kubera ko urumuri rwatatanye kuruhande rwicyuma cyoroshye rushobora kuba ruri muri capillary utitaye kumpande zibyabaye. Kwishyira hamwe nkibice 5.12 nM birashobora kugerwaho ukoresheje reagent zisanzwe IMANA-POD tubikesha uburyo bushya butari umurongo wa optique amplification hamwe na sample yihuta yo guhinduranya no gutahura glucose. Iyi fotometer yoroheje kandi ihendutse izakoreshwa cyane mubumenyi bwubuzima no gukurikirana ibidukikije kugirango isesengurwe.
Nkuko bigaragara ku gishushanyo cya 1, Photometer ishingiye kuri MWC igizwe na cm 7 z'uburebure bwa MWC (diameter y'imbere 1,7 mm, diameter yo hanze ya mm 3.18, EP yo mu rwego rwa elegitoronike y’imbere, SUS316L ibyuma bitagira umuyonga), uburebure bwa 505 nm LED (Thorlabs M505F1), hamwe na lens (urumuri rukwirakwiza hafi ya dogere 6,6) in / hanze. T-umuhuza bikorwa muguhuza isahani ya quartz ibonerana kumuyoboro wa PMMA urimo MWC na Peek (ID 0,72 mm ID, 1,6 mm OD, Vici Valco Corp.) winjizwemo cyane kandi uhambiriwe. Inzira yinzira eshatu ihujwe na Pike inlet tube ikoreshwa muguhindura icyitegererezo cyinjira. Photodetector irashobora guhindura ingufu za optique yakiriwe P mukongera imbaraga za voltage N × V (aho V / P = 1.0 V / W kuri 1550 nm, inyungu N irashobora guhindurwa nintoki hagati ya 103-107). Kubugufi, V ikoreshwa aho kuba N × V nkibimenyetso bisohoka.
Mugereranije, ecran yubucuruzi (Agilent Technologies Cary 300 serie hamwe na R928 High Efficiency Photomultiplier) hamwe na selile cuvette ya cm 1.0 nayo yakoreshejwe mugupima iyakirwa ryikigereranyo cyamazi.
Ubuso bw'imbere bwa MWC bwaciwe bwasuzumwe hifashishijwe imiterere ya optique (ZYGO New View 5022) ifite vertical na verisiyo ya 0.1 nm na 0.11 µm.
Imiti yose (urwego rwo gusesengura, nta yandi masuku) yaguzwe muri Sichuan Chuangke Biotechnology Co., Ltd. Ibikoresho bipimisha glucose birimo glucose oxydease (IMANA), peroxidase (POD), 4-aminoantipyrine na fenol, nibindi. Igisubizo cya chromogeneque cyateguwe nuburyo busanzwe IMANA-POD 37.
Nkuko bigaragara mu mbonerahamwe ya 2, urutonde rwibisubizo bya glucose mubitekerezo bitandukanye byateguwe hifashishijwe DI H2O nkumuyoboro ukoresheje uburyo bwo gukwirakwiza ibintu (reba Ibikoresho byiyongera kubisobanuro birambuye). Tegura ingero zanduye cyangwa zidafite ishingiro uvanga glucose cyangwa amazi ya deionion hamwe nigisubizo cya chromogenic mugipimo cyagenwe cya 3: 1. Ibyitegererezo byose byabitswe kuri 37 ° C bikingiwe urumuri muminota 10 mbere yo gupimwa. Muburyo bw'IMANA-POD, ingero zijimye zihinduka umutuku hamwe no kwinjiza ntarengwa kuri 505 nm, kandi iyinjizwa ryayo rihwanye nubunini bwa glucose.
Nkuko bigaragara mu mbonerahamwe ya 1, urukurikirane rwibisubizo bitukura (Ostrich Ink Co., Ltd., Tianjin, Ubushinwa) byateguwe nuburyo bwo gukwirakwiza serivise hakoreshejwe DI H2O nkibisubizo.
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