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Tsvagiridzo yekuongorora yemasampuli emvura ine huwandu hwakasiyana hwemashandisirwo musainzi yehupenyu uye kuongorora kwezvakatipoteredza. Mubasa iri, isu takagadzira compact uye isingadhure photometer yakavakirwa pasimbi waveguide capillaries (MCCs) yekusimbisa kutsunga kwekunyura. Iyo nzira yemaziso inogona kuwedzerwa zvakanyanya, uye yakareba kupfuura kureba kwemuviri kweMWC, nekuti mwenje wakaparadzirwa nemarara akatsetseka simbi pamativi anogona kuve mukati mecapillary zvisinei nekona yechiitiko. Concentrations yakaderera se5.12 nM inogona kuwanikwa uchishandisa zvakajairika chromogenic reagents nekuda kweiyo nyowani isiri-linear optical amplification uye nekukurumidza sampuli switch uye kuona glucose.
Photometry inoshandiswa zvakanyanya kutsvaga kuongororwa kwemasampuli emvura nekuda kwekuwanda kweanowanikwa chromogenic reagents uye semiconductor optoelectronic zvishandiso1,2,3,4,5. Kuenzaniswa neyechinyakare cuvette-based absorbance determination, liquid waveguide (LWC) capillaries reflect (TIR) ​​​​nekuchengeta probe chiedza mukati me capillary1,2,3,4,5. Zvisinei, pasina humwe huri nani hunovandudzwa, nzira yemaziso inongoswedera pedyo nehurefu hwemuviri weLWC3.6, uye kuwedzera kweLWC kureba kupfuura 1.0 m ichatambura nekudzivirira kwechiedza chakasimba uye ngozi yakawanda yebubbles, etc.3, 7. Nezvesero yakarongedzerwa yakawanda-reflection sero yekuvandudza nzira ye optical, muganhu wekuona unongovandudzwa chete nechikamu che8.95.
Parizvino kune mhando mbiri huru dzeLWC, dzinoti Teflon AF capillaries (ine refractive index ye ~ 1.3 chete, iyo yakaderera pane iyo yemvura) uye silica capillaries yakaputirwa neTeflon AF kana simbi mafirimu1,3,4. Kuti uwane TIR pakusangana pakati pezvinhu zvedielectric, zvinhu zvine yakaderera refractive index uye high light incidence angles zvinodiwa3,6,10. Nezve Teflon AF capillaries, Teflon AF inofema nekuda kweiyo porous chimiro3,11 uye inogona kutora zvidiki zvezvinhu mumasampuli emvura. Kune quartz capillaries yakavharwa kunze neTeflon AF kana simbi, iyo refractive index yequartz (1.45) yakakwirira kudarika yakawanda yemvura samples (eg 1.33 yemvura)3,6,12,13. Kune ma capillaries akaputirwa nesimbi firimu mukati, zvifambiso zvekutakura zvakadzidzwa14,15,16,17,18, asi nzira yekuputira yakaoma, pamusoro pefirimu resimbi ine rough uye porous structure4,19.
Pamusoro pezvo, maLWC ezvokutengeserana (AF Teflon Coated Capillaries uye AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) ane zvimwe zvipingamupinyi, se: pakukanganisa. . Iyo yakakura yakafa volume yeTIR3,10, (2) T-connector (kubatanidza capillaries, fibers, uye inlet/outlet tubes) inogona kuteya mabubble emhepo10.
Panguva imwecheteyo, kutsunga kwemazinga eglucose kwakakosha zvikuru kuongororwa kwechirwere cheshuga, cirrhosis yechiropa uye chirwere chepfungwa20. uye nzira dzakawanda dzekuona dzakaita sephotometry (kusanganisira spectrophotometry 21, 22, 23, 24, 25 uye colorimetry pabepa 26, 27, 28), galvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, optical plasmon 37, Fabry-Perot cavity 38, electrochemistry 39 uye capillary electrophoresis 40,41 zvichingodaro. Zvisinei, dzakawanda dzeidzi nzira dzinoda midziyo inodhura, uye kuonekwa kweglucose pane dzakawanda nanomolar concentrations inoramba iri dambudziko (somuenzaniso, kune photometric zviyero21, 22, 23, 24, 25, 26, 27, 28, iyo yakaderera yeglucose). iyo inogumira yaingova 30 nM apo Prussian yebhuruu nanoparticles yakashandiswa se peroxidase mimics). Nanomolar glucose ongororo inowanzodikanwa kune ma molecular-level cellular zvidzidzo senge inhibition yekukura kwegomarara reprostate42 uye CO2 kugadzirisa maitiro eProchlorococcus mugungwa.
Muchinyorwa chino, compact, isingadhure photometer yakavakirwa pasimbi waveguide capillary (MWC), SUS316L Stainless simbi capillary ine electropolished yemukati pamusoro, yakagadziridzwa kuti ultrasensitive absorption kutsunga. Sezvo mwenje uchigona kuvharirwa mukati mesimbi capillaries zvisinei nekona yechiitiko, nzira yemaziso inogona kuwedzerwa zvakanyanya nekupararira kwechiedza pamarara uye akatsetseka esimbi, uye yakareba kupfuura kureba kwemuviri kweMWC. Uye zvakare, yakapfava T-connector yakagadzirirwa iyo yekuona yekubatanidza uye fluid inlet/outlet kudzikisa vhoriyamu yakafa uye kudzivirira bubble entrapment. Kune iyo 7 cm MWC photometer, muganho wekuona unovandudzwa nekanosvika 3000 nguva kana ichienzaniswa neyekutengesa spectrophotometer ine 1 cm cuvette nekuda kwekuvandudzwa kutsva kweiyo isiri-mutsara nzira yekuona uye nekukurumidza sampuli switch, uye kutariswa kweglucose kunogona kuwanikwa zvakare. chete 5.12 nM uchishandisa zvakajairika chromogenic reagents.
Sezvinoratidzwa muMufananidzo 1, iyo MWC-yakavakirwa photometer ine 7 cm kureba MWC ine EP giredhi electropolished mukati mepasi, 505 nm LED ine lens, inogadzirika kuwana photodetector, uye maviri ekubatanidza optical uye yekuisa mvura. Buda. Ivhavha yenzira nhatu yakabatana nePike inlet chubhu inoshandiswa kushandura sampu iri kuuya. Iyo Peek chubhu inokwana zvakanaka inopesana neiyo quartz ndiro uye MWC, saka iyo yakafa vhoriyamu muT-connector inochengetwa iri shoma, zvinobudirira kudzivirira mabhuru emhepo kubva pakubatwa. Uye zvakare, iyo collimated danda inogona kuunzwa zviri nyore uye nemazvo muMWC kuburikidza neT-chidimbu quartz ndiro.
Iyo danda uye sampu yemvura inounzwa muMCC kuburikidza neT-chidimbu, uye danda rinopfuura nepakati peMCC rinogamuchirwa nephotodetector. Mhinduro dzinouya dzematavi kana asina sampuli dzakaunzwa mune imwe ICC kuburikidza nevhavha yenzira nhatu. Zvinoenderana nemutemo weBeer, iyo optical density yemuenzaniso weruvara inogona kuverengerwa kubva kuequation. 1.10
uko Vcolor neVblank ndiwo masaini anobuda eiyo photodetector kana mavara uye masampuli asina chinhu akaunzwa muMCC, zvichiteerana, uye Vdark ndiyo chiratidzo chekumashure chephotodetector kana LED yakadzimwa. Shanduko muchiratidzo chekubuda ΔV = Vcolor-Vblank inogona kuyerwa nekuchinja sampuli. Maererano ne equation. Sezvinoratidzwa muMufananidzo 1, kana ΔV iri duku kudarika Vblank-Vdark, paunenge uchishandisa sampling switching scheme, kuchinja kuduku muVblank (eg drift) kunogona kuita zvishoma pane AMWC kukosha.
Kuenzanisa mashandiro eMWC-based photometer necuvette-based spectrophotometer, mhinduro yeinki tsvuku yakashandiswa semuenzaniso weruvara nekuda kwekugadzikana kwayo kweruvara uye yakanaka concentration-absorbance linearity, DI H2O semuenzaniso usina chinhu. . Sezvinoratidzwa muTable 1, nhevedzano yeingi tsvuku mhinduro dzakagadzirwa neiyo serial dilution nzira uchishandisa DI H2O se solvent. Iyo yehukama yekumisikidzwa kwemuenzaniso 1 (S1), isina kunyorwa pendi tsvuku yepakutanga, yakatemwa se 1.0. Pamusoro pemuonde. Mufananidzo 2 unoratidza optical mafoto e11 tsvuku inki samples (S4 kusvika S14) ine hukama hwehukama (yakanyorwa muTable 1) kubva ku8.0 × 10-3 (kuruboshwe) kusvika 8.2 × 10-10 (kurudyi).
Migumisiro yekuyera yemuenzaniso 6 inoratidzwa muFig. 3(a). Iwo mapoinzi ekuchinja pakati peakasvibiswa uye asina sampuli masampuli akaiswa mumufananidzo neaviri miseve "↔". Zvinogona kuoneka kuti simba rekubuda rinowedzera nekukurumidza kana uchichinja kubva kumavara emhando kuenda kune isina chinhu masampuli uye zvinopesana. Vcolor, Vblank uye inowirirana ΔV inogona kuwanikwa sezvinoratidzwa mumufananidzo.
(a) Kuyerwa kwemhedzisiro yemuenzaniso 6, (b) sampuli 9, (c) sampuli 13, uye (d) sampuli 14 uchishandisa MWC-based photometer.
Mhedzisiro yezviyero zvemasampuli 9, 13, uye 14 inoratidzwa muFig. 3(b)-(d), zvichiteerana. Sezvinoratidzwa muMufananidzo 3 (d), yakayerwa ΔV inongova 5 nV, iyo inenge 3 nguva yeruzha runokosha (2 nV). Idiki ΔV yakaoma kusiyanisa kubva kune ruzha. Nokudaro, muganhu wekutsvaga wakasvika kune imwe nzvimbo ye8.2 × 10-10 (sampuli 14). Nerubatsiro rwe equations. 1. AMWC absorbance inogona kuverengwa kubva kuyerwa Vcolor, Vblank uye Vdark values. Kune photodetector ine pfuma ye104 Vdark ndeye -0.68 μV. Mhedzisiro yezviyero zveese masampuli inopfupikiswa muTafura 1 uye inogona kuwanikwa mune yekuwedzera zvinhu. Sezvinoratidzwa muTebhurari 1, kunyura kunowanikwa munzvimbo dzakanyanya kuzara, saka kunyura pamusoro pe3.7 hakugone kuyerwa neMWC-based spectrometers.
Kuenzanisa, sampu yeinki tsvuku yakayerwawo ne spectrophotometer uye kuyerwa kweAcuvette absorbance inoratidzwa muMufananidzo 4. Maitiro eAcuvette pa 505 nm (sezvinoratidzwa muTebhurari 1) akawanikwa kuburikidza nekutaura kune curves yemasampuli 10, 11, kana 12 (sezvinoratidzwa muchikamu). kusvika Fig. 4) sechinhu chekutanga. Sezvinoratidzwa, muganho wekuona wakasvika kune imwe nhanho ye2.56 x 10-6 (sample 9) nekuti absorption curves yemasampuli 10, 11 uye 12 aive akasarudzika kubva kune mumwe nemumwe. Saka, pakushandisa iyo MWC-based photometer, muganho wekuona wakagadziridzwa nechikamu che3125 zvichienzaniswa necuvette-based spectrophotometer.
Dependence absorption-concentration inoratidzwa muFig.5. Kune zviyero zve cuvette, kunyura kunoenzana neinki yekumisikidza panzira yakareba 1 cm. Ipo, kune MWC-yakavakirwa zviyero, kuwedzera kusiri-mutsara kwekutora kwakaonekwa kune yakaderera. Zvinoenderana nemutemo weBeer, kunyura kunoenderana nekureba kwenzira, saka kunyura kunowana AEF (inotsanangurwa seAEF = AMWC/Acuvette pane imwecheteyo inki concentration) ireshiyo yeMWC kune iyo optical nzira kureba kwecuvette. Sezvinoratidzwa muFigure 5, pakukwirira kwakanyanya, iyo AEF inogara yakatenderedza 7.0, izvo zvine musoro sezvo kureba kweMWC kuri kwakapetwa ka7 kureba kwe1 cm cuvette. Zvisinei, pazvikamu zvakaderera (zvine hukama <1.28 × 10-5), AEF inowedzera nekuderera kwekugadzirisa uye yaizosvika kukosha kwe803 pahutano hwakabatana hwe8.2 × 10-10 nekuwedzera curve ye cuvette-based measurement. Zvisinei, pazvikamu zvakaderera (zvine hukama <1.28 × 10-5), AEF inowedzera nekuderera kwekugadzirisa uye yaizosvika kukosha kwe803 pahutano hwakabatana hwe8.2 × 10-10 nekuwedzera curve ye cuvette-based measurement. Однако при низких концентрациях (относительная концентрация <1,28 × 10–5) AEF увеличивается с уменьшением концентразентрация 803 при относительной концентрации 8,2 × 10–10 при экстраполяции кривой измерения на основе кюветы. Zvisinei, pazvikamu zvakaderera (zvichienderana nehuwandu <1.28 × 10-5), AEF inowedzera nekuderera kwekugadzirisa uye inogona kusvika kuhuwandu hwe803 pane imwe nzvimbo ye8.2 × 10-10 kana yakabudiswa kubva kune cuvette-based measurement curve.然而，在低浓度（相关浓度<1.28 × 10-5 ）下，AEF随着浓度的降低而增加，并且通过外推基于比色皿的测量曲线，在相关浓度為8.2 × 3 × 8.2 × 3 × 8.2 ×的值.然而 ， 在 低 浓度 （相关 浓度 <1.28 × 10-5） ， , AEF 随着 的 降低 而 , 并低 并万 通迎比色皿 测量 曲线 ， 在 浓度 為 8.2 × 10-10 时 达到 达到 达到 达到803 值. Однако при низких концентрациях (релевантные концентрации < 1,28 × 10-5) АЭП увеличивается с уменьшением концентрации 8,2 × 10–10 803 . Zvisinei, pazvikamu zvakaderera (zvakanyanya kukosha <1.28 × 10-5) AED inowedzera nekuderera kwekugadzirisa, uye kana ichibudiswa kubva kune cuvette-based based measurement curve, inosvika pakukosha kwehuwandu hwe8.2 × 10-10 803.Izvi zvinoguma nenzira inoenderana yekuona ye803 cm (AEF × 1 cm), iyo yakareba kupfuura kureba kwemuviri kweMWC, uye yakatoreba kupfuura iyo yakareba inotengeswa yeLWC (500 cm kubva kuWorld Precision Instruments, Inc.). Doko Engineering LLC ine kureba kwe200 cm). Uku kusiri-mutsara kuwedzera kwekunyura muLWC hakuna kumbotaurwa.
Pamusoro pemuonde. 6(a)-(c) inoratidza mufananidzo wemaziso, mufananidzo wemicroscope, uye mufananidzo weoptical profiler wemukati mechikamu cheMWC, zvichiteerana. Sezvinoratidzwa mufig. 6(a), iyo yemukati yakatsetseka uye inopenya, inogona kuratidza chiedza chinooneka, uye inonyatso ratidza. Sezvinoratidzwa mufig. 6(b), nekuda kwekuremara uye chimiro chekristaro chesimbi, mesas madiki uye zvisizvo zvinoonekwa panzvimbo yakatsetseka. Mukuona kwenzvimbo duku (<5 μm×5 μm), kushata kwepamusoro pepamusoro pasi pe1.2 nm (Fig. 6 (c)). Mukuona kwenzvimbo duku (<5 μm × 5 μm), kushata kwepamusoro pepamusoro pasi pe1.2 nm (Fig. 6 (c)). Ввиду малой площади (<5 мкм×5 мкм) шероховатость большей части поверхности составляет менее 1,2 нм (рис. 6(в)). Pamusana penzvimbo duku (<5 µm×5 µm), kushata kwehuwandu hwepamusoro huri pasi pe1.2 nm (Fig. 6 (c)).考虑到小面积（<5 μm×5 μm），大多数表面的粗糙度小于1.2 nm（图6（c）).考虑到小面积（<5 μm×5 μm），大多数表面的粗糙度小于1.2 nm（图6（c）). Учитывая небольшую площадь (<5 мкм × 5 мкм), шероховатость большинства поверхностей составляет менее 1,2 нм (рис. Tichifunga nezvenzvimbo diki (<5 µm × 5 µm), kushata kwenzvimbo dzakawanda pasi pe1.2 nm (Fig. 6 (c)).
(a) Optical image, (b) microscope image, uye (c) optical image yemukati meiyo MWC cut.
Sezvinoratidzwa mufig. 7. Kune Teflon AF capillaries yakazadzwa neDI H2O, kona yezviitiko inofanira kuva yakakura kudarika yakakosha angle ye 77.8 °, saka LOP iri pasi pe1.02 × LC pasina imwe kuvandudzwa3.6. Nepo, neMWC, kuvharirwa kwechiedza mukati me capillary kwakasununguka kune refractive index kana angle yezviitiko, kuitira kuti kona yechiitiko ichiderera, nzira yechiedza inogona kureba kupfuura kureba kwecapillary (LOP »LC). Sezvinoratidzwa mufig. 7 (b), iyo corrugated simbi pamusoro inogona kukonzeresa kupararira kwechiedza, izvo zvinogona kuwedzera zvakanyanya nzira yekuona.
Naizvozvo, kune nzira mbiri dzemwenje dzeMWC: mwenje wakananga usingaratidzi (LOP = LC) uye mwenje we sawtooth une maratidziro mazhinji pakati pemadziro eparutivi (LOP »LC). Zvinoenderana nemutemo weBeer, kusimba kweiyo yakafambiswa yakananga uye zigzag mwenje inogona kuratidzwa sePS × exp (-α × LC) uye PZ × exp (-α × LOP) zvichiteerana, apo iyo inogara α ndiyo inonyudza coefficient, iyo inoenderana zvachose neingi.
Nekukwirisa inki yekumisikidza (eg, yakabatana concentration> 1.28 × 10-5), iyo zigzag-mwenje inodzikisirwa zvakanyanya uye kusimba kwayo kwakadzikira pane iyo yakatwasuka-chiedza, nekuda kwehombe yekunyudza-coefficient uye yayo yakareba optical-nzira. Pamusoro peinki yepamusoro (semuenzaniso, yakabatana > 1.28 × 10-5), iyo zigzag-chiedza inodzikiswa zvakanyanya uye kusimba kwayo kwakadzikira pane iyo yemwenje-yakatwasuka, nekuda kwehombe yekunyudza-coefficient uye yayo yakareba optical- nzira. Высокой концентрацией (например, относительная концентрация >1,28 × 10-5) зигзагообразный свет силььено затунся намного ниже, чем у прямого света, из-за большого коэфффициента поглощения и гораздо более длинного оптического излучения. Nekukwirisa inki yekumisikidza (eg hama concentration> 1.28 × 10-5), mwenje we zigzag wakadzikiswa zvakanyanya uye kusimba kwaro kwakadzikira pane kuya kwechiedza chakananga nekuda kweiyo hombe yekunyudza coefficient uye yakareba kwenguva refu optical emission.track.对于高浓度墨水（例如，相关浓度>1.28×10-5），Z字形光衰减很大，其强度远低于直光，這是由于吸收系数大，光学时间更长.对于 高浓度 墨水 （例如 , 浓度 浓度> 1.28 × 10-5） , z 字形 衰减 很 大 ， , 這 是 吸收 系数 大 光学 时间 更。。 长 长 长 长 长 长 长 长 长Для чернил с высокой концентрацией (например, релевантные концентрации >1,28×10-5) намного ниже, чем у прямого света из-за большого коэфффициента поглощения и более длительного оптического времени. Kune inki dzepamusoro (semuenzaniso, kukoshesa kwakakodzera> 1.28 × 10-5), chiedza chezigzag chakanyanya kuderedzwa uye kusimba kwaro kwakaderera zvikuru kudarika kwechiedza chakananga nekuda kwekofi yakakura yekutora uye nguva yakareba yemaziso.nzira shoma.Nokudaro, chiedza chakananga chaitonga kugadzirisa kugadzirisa (LOP = LC) uye AEF yakachengetwa nguva dzose pa ~ 7.0. Kusiyana neizvi, apo kunyura-coefficient kwakaderera nekuderera kweinki kuisa (semuenzaniso, kushamwaridzana kwakabatana <1.28 × 10-5), kusimba kwe-zigzag-chiedza kunowedzera nokukurumidza kupfuura iyo ye-light-light uye ipapo zigzag-light inotanga kuita basa rinokosha. Kusiyana neizvi, apo kunyura-coefficient kwakaderera nekuderera kweinki kuisa (semuenzaniso, kushamwaridzana kwakabatana <1.28 × 10-5), kusimba kwe-zigzag-chiedza kunowedzera nokukurumidza kupfuura iyo ye-light-light uye ipapo zigzag-light inotanga kuita basa rinokosha. Напротив, когда коэффициент поглощения уменьшением концентрации чернил (например, относительная концент,2085), × интенсивность зигзагообразного света увеличивается быстрее, чем у прямого света, и затем начинает играть зигзагообразный свет. Kusiyana neizvi, kana iyo coefficient yekunyura inoderera nekuderera kweinki yekumisikidza (somuenzaniso, hukama hwehukama <1.28 × 10-5), kusimba kwechiedza chezigzag kunowedzera nekukurumidza kupfuura kwechiedza chakananga, uye ipapo zigzag chiedza chinotanga kutamba.basa rinokosha.相反，当吸收系数随着墨水浓度的降低而降低时（例如，相关浓度<1.28×10-5 ），字形光的强度比直光增加得更快，然后Z字形光开始发挥作用一个更重要的角色.相反 , 当 吸收 系数 随着 墨水 的 降低 而 降低 时 例如 例如 , 相关 浓度 1 × 1 × 1. , 字形光 的 强度 比 增加 得 更 , 然后 z 字形光 发挥 作用 一个 重要 重要 重要活更 .更 HI的角色. И наборот, когда коэффициент поглощения уменьшается с уменьшением концентрации чернил (например, соответствуюю концен1-1×1),

интенсивность зигзагообразного света увеличивается быстрее, чем прямого, и тогда зигзагообразный свет начинает играть более важную. Sezvineiwo, kana kunyura kuderera kunoderera nekuderera kweinki yekumisikidza (somuenzaniso, iyo inoenderana neiyo < 1.28 × 10-5), kusimba kwechiedza che zigzag kunowedzera nekukurumidza kupfuura mwenje wakananga, uye ipapo mwenje we zigzag unotanga kuita basa rakakosha.hunhu.Naizvozvo, nekuda kweiyo sawtooth optical nzira (LOP »LC), iyo AEF inogona kuwedzerwa zvakanyanya kupfuura 7.0. Chaiyo chiedza chekufambisa hunhu cheMWC chinogona kuwanikwa uchishandisa waveguide mode theory.
Pamusoro pekuvandudza nzira yekuona, kukurumidza kushandura sampuli kunopawo kune yekupedzisira-yakaderera yekuona miganhu. Nekuda kwevhoriyamu diki yeMCC (0.16 ml), nguva inodiwa kuchinja nekuchinja mhinduro muMCC inogona kuva isingasviki 20 seconds. Sezvinoratidzwa mumufananidzo 5, iyo shoma inoonekwa kukosha kwe AMWC (2.5 × 10-4) yakaderera ka4 pane iyo yeAcuvette (1.0 × 10-3). Iko kukurumidza kushandura kwemhinduro inoyerera mu capillary kunoderedza mhedzisiro yeruzha rwehurongwa (semuenzaniso kudonha) pakurongeka kwemusiyano wekunyudza uchienzaniswa nemhinduro yekuchengetedza mucuvette. Somuenzaniso, sezvinoratidzwa mufig. 3 (b) - (d), ΔV inogona kusiyaniswa nyore nyore kubva kune chiratidzo chekukukurwa nekuda kwekutsanya sampuli kuchinjika mudiki vhoriyamu capillary.
Sezvinoratidzwa muTable 2, huwandu hwemhinduro dzeglucose pane dzakasiyana siyana dzakagadzirirwa uchishandisa DI H2O se solvent. Sampuli dzakasvibiswa kana dzisina chinhu dzakagadzirwa nekusanganisa glucose mhinduro kana mvura yakasvibiswa ine chromogenic solutions yeglucose oxidase (GOD) uye peroxidase (POD) 37 muhuwandu hwehuwandu hwehuwandu hwe3: 1, maererano. Pamusoro pemuonde. 8 inoratidza mapikicha ekuona emasampuri ane pfumbamwe (S2-S10) ane glucose concentration kubva pa2.0 mM (kuruboshwe) kusvika 5.12 nM (kurudyi). Kutsvuka kunoderera nekuderera kweglucose concentration.
Mhedzisiro yezviyero zvemasampuli 4, 9, uye gumi ine MWC-yakavakirwa photometer inoratidzwa muFig. 9(a)-(c), zvichiteerana. Sezvinoratidzwa mufig. 9 (c), iyo yakayerwa ΔV inova isina kugadzikana uye zvishoma nezvishoma inowedzera panguva yekuyera sezvo ruvara rweMWARI-POD reagent pachayo (kunyange pasina kuwedzera glucose) zvishoma nezvishoma kuchinja muchiedza. Nokudaro, kutevera ΔV zviyero hazvigoni kudzokororwa kune sampuli neglucose concentration yepasi pe5.12 nM (sample 10), nokuti kana ΔV iri duku zvakakwana, kusagadzikana kweMWARI-POD reagent haigoni kuregererwa. Nokudaro, muganhu wekuonekwa kweglucose solution ndeye 5.12 nM, kunyange iyo inowirirana ΔV kukosha (0.52 µV) yakakura kudarika kukosha kweruzha (0.03 µV), zvichiratidza kuti ΔV duku inogona kuonekwa. Uyu muganho wekuona unogona kuvandudzwa nekushandisa zvakanyanya kugadzikana chromogenic reagents.
(a) Kuyerwa kwemhedzisiro yemuenzaniso 4, (b) sampuli 9, uye (c) sampuli gumi uchishandisa MWC-based photometer.
Iyo AMWC absorbance inogona kuverengerwa uchishandisa yakayerwa Vcolor, Vblank uye Vdark kukosha. Kune photodetector ine pfuma ye105 Vdark ndeye -0.068 μV. Zviyero zveese masampuli zvinogona kuiswa mune yekuwedzera zvinhu. Kuenzanisa, masampuli eglucose akayerwawo ne spectrophotometer uye kuyerwa kunyura kweAcuvette kwakasvika padanho rekuonekwa re0.64 µM (sample 7) sezvakaratidzwa mumufananidzo 10.
Ukama huri pakati pekutora uye kuisa pfungwa hunoratidzwa muMufananidzo 11. NeMWC-based photometer, 125-fold kuvandudzwa kwemuganhu wekutsvaga wakawanikwa uchienzaniswa necuvette-based spectrophotometer. Kuvandudzwa uku kwakaderera pane iyo tsvuku inki assay nekuda kwekusagadzikana kwakashata kweMWARI-POD reagent. Kuwedzera kusiri mutsara mukunyura pazvishoma zvakaonekwa zvakare.
Iyo MWC-yakavakirwa photometer yakagadziridzwa kuti ionekwe kwekupedzisira-kunzwa kwemasampuli emvura. Iyo nzira yemaziso inogona kuwedzerwa zvakanyanya, uye yakareba kupfuura kureba kwemuviri kweMWC, nekuti mwenje wakaparadzirwa nemarara akatsetseka simbi pamativi anogona kuve mukati mecapillary zvisinei nekona yechiitiko. Concentrations yakaderera se 5.12 nM inogona kuwanikwa uchishandisa zvakajairwa MWARI-POD reagents nekuda kweiyo nyowani isiri-mutsara yekutarisa amplification uye nekukurumidza sampuli switch uye kuona glucose. Iyi compact uye isingadhure photometer ichashandiswa zvakanyanya musainzi yehupenyu uye yekutarisa kwezvakatipoteredza kuti iongorore kuongorora.
Sezvinoratidzwa mumufananidzo 1, iyo MWC-based photometer ine 7 cm kureba kweMWC (yemukati dhayamita 1.7 mm, kunze dhayamita 3.18 mm, EP kirasi electropolished mukati mepamusoro, SUS316L Stainless simbi capillary), 505 nm wavelength LED (Thorlabs M505M505F6be6be), uye madhigirii ekupararira kweiyo 6be. (Thorlabs PDB450C) uye maviri T-zvikonikoni zvekuona kutaurirana uye mvura mukati / kunze. I-T-connector inogadzirwa nekubatanidza quartz plate inoonekera kune PMMA chubhu umo MWC uye Peek machubhu (0.72 mm ID, 1.6 mm OD, Vici Valco Corp.) akaiswa zvakasimba uye akanamwa. Ivhavha yenzira nhatu yakabatana nePike inlet chubhu inoshandiswa kushandura sampu iri kuuya. Iyo photodetector inogona kushandura iyo yakagamuchirwa optical simba P kuita yakawedzera voltage chiratidzo N× V (apo V / P = 1.0 V / W pa1550 nm, kuwana N inogona kugadziriswa nemaoko muhuwandu hwe103-107). Nekupfupika, V inoshandiswa pachinzvimbo cheN× V sechiratidzo chekubuda.
Mukuenzanisa, yekutengeserana spectrophotometer (Agilent Technologies Cary 300 series ine R928 High Efficiency Photomultiplier) ine 1.0 cm cuvette cell yakashandiswawo kuyera kunwa kwemasampuli emvura.
Nzvimbo yemukati yeMWC yakachekwa yakaongororwa uchishandisa optical surface profiler (ZYGO New View 5022) ine vertical and lateral resolution ye0.1 nm uye 0.11 µm, zvichiteerana.
Ese makemikari (analytical giredhi, hapana kumwe kucheneswa) akatengwa kubva kuSichuan Chuangke Biotechnology Co., Ltd. Glucose test kits inosanganisira glucose oxidase (MWARI), peroxidase (POD), 4-aminoantipyrine uye phenol, nezvimwewo. Mushonga wechromogenic wakagadzirirwa nenzira yakajairika MWARI-POD 37 nzira.
Sezvinoratidzwa muTable 2, huwandu hwemhinduro dzeglucose pane dzakasiyana siyana dzakagadzirirwa uchishandisa DI H2O sediluent uchishandisa serial dilution nzira (ona Supplementary Materials kuti uwane rumwe ruzivo). Gadzirira sampuli dzakasvibiswa kana dzisina kuvharwa nekusanganisa glucose solution kana mvura yakasvibiswa nechromogenic solution muhuwandu hwehuwandu hwehuwandu hwe3: 1, zvichiteerana. Mienzaniso yese yakachengetwa pa37 ° C yakadzivirirwa kubva pachiedza kwemaminitsi gumi isati yayera. Munzira yaMWARI-POD, masampuli ane mavara anoshanduka akatsvuka ane absorption yakakura pa505 nm, uye kunyura kunenge kwakaenzana neiyo glucose concentration.
Sezvinoratidzwa muTebhurari 1, nhevedzano yeingi tsvuku mhinduro (Ostrich Ink Co., Ltd., Tianjin, China) dzakagadzirirwa ne serial dilution nzira uchishandisa DI H2O se solvent.
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